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The map label for this gene is ispG

Identifier: 21242544

GI number: 21242544

Start: 2072386

End: 2073651

Strand: Direct

Name: ispG

Synonym: XAC1799

Alternate gene names: 21242544

Gene position: 2072386-2073651 (Clockwise)

Preceding gene: 21242541

Following gene: 21242545

Centisome position: 40.04

GC content: 66.27

Gene sequence:

>1266_bases
ATGCATGACGCCGTGACCCGCCCAACCCCTCCCGCCGACGCCACCGCCTGGCCGCGCCGCATCACCCGGGCCGTCAAGAT
CGGCAGCGTGACCGTGGGCGGGGGCCATCCGGTCGTCGTCCAGTCGATGACCAACACCGACACCGCCGACATCGCCGGCA
GCGTCAAGCAGGTCGCCGATCTGTGGCGCGCCGGCTCGGAAATGGTGCGCCTGACCGTCAACAATGCCGAGTCCGCTGCT
GCCATTCCGCGCATCGTCGACAAGCTGCGCATGATGGGCATCGAAGTGCCGTTGATCGGCGACTTCCATTACAACGGCCA
TCAATTGCTCGCTGCCGAGCCGGCCTGTGCCGAGGCGCTGGCCAAGTACCGCATCAACCCGGGCAATGTCGGATTCGGCA
AGAAGAAGGATCTGCAGTTCGGTCAGTTGATCGAGTTCGCCATCAAGTACGGCAAGCCGGTCCGGATTGGTGCCAACTGG
GGCTCGCTGGACCAGTCGCTGGCAGCGCAGCTGATGGACGAAAACTCGCAACGCGACACCCCGTGGGACGCCGGCCGGGT
GCTGCGCGAGGCGCTGATCCGCTCGGCGGTGGATTCGGCCGAGCGTGCGGTGGAGCTGGGCCTGCCGCGCGAGCGCATCA
TCCTGTCGGCCAAGGTGTCCGGCGTGCAGGAGCTGATCGCGGTATATCGGGACATGGCCAGCCGCTGCGATTTCGCGCTG
CATCTGGGGCTCACCGAAGCCGGCATCGGCAGCAAGGGCATCGTGGCTTCGGCGGCGGCACTGAGCGTGCTGTTGCAGGA
AGGCATCGGCGACACCATCCGTATTTCGCTGACCCCCGAGCCGGGGCAGTCGCGCACCCAGGAAGTGGTGGTGGCGCAGG
AACTGTTGCAGACCACCGGCCAGCGCGCGTTTACCCCGATGGTCACCGCCTGCCCGGGCTGCGGCCGCACCACCTCCGAG
TTCTTCCAGGAGCTGGCCGGCGTGGTGCAGAACCACGTGCGCGCCAAGATGCCGGAGTGGAAGATCACCAACCCCGGCGC
GGAAAACATGACCCTGGCGGTGATGGGCTGCGTGGTCAACGGGCCGGGCGAATCGCGCCACGCCAATATCGGTATTTCGC
TGCCGGGCACGGGCGAGGCACCGTCGGCGCCAGTGTTCATCGACGGCGAAAAGAGCGTCACCTTGCGCGGCGAAAACATC
GCGTACGAATTCATCGAGTTGATCGATCAATACGTCGAACGCACCTATGTCCGCCGCGCCGGCTGA

Upstream 100 bases:

>100_bases
GGGCGCTTCGCACTCACCTCAACGTACATGACACGCTCTCCAGATCGGTGCGCATCCCGCTTCAGCTGTGAGCGCACGGC
GGACAGGCTAAAATAGCCCG

Downstream 100 bases:

>100_bases
ATCGCCGGCCGGGTTCAAGGGATGGTTGCGTAGCAATGCGTGGCGCATGGCGTTGCTGTTTGCCGGGGTGCTGCTCCCAT
TGGGCCTGTTCGTGGACCTG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MHDAVTRPTPPADATAWPRRITRAVKIGSVTVGGGHPVVVQSMTNTDTADIAGSVKQVADLWRAGSEMVRLTVNNAESAA
AIPRIVDKLRMMGIEVPLIGDFHYNGHQLLAAEPACAEALAKYRINPGNVGFGKKKDLQFGQLIEFAIKYGKPVRIGANW
GSLDQSLAAQLMDENSQRDTPWDAGRVLREALIRSAVDSAERAVELGLPRERIILSAKVSGVQELIAVYRDMASRCDFAL
HLGLTEAGIGSKGIVASAAALSVLLQEGIGDTIRISLTPEPGQSRTQEVVVAQELLQTTGQRAFTPMVTACPGCGRTTSE
FFQELAGVVQNHVRAKMPEWKITNPGAENMTLAVMGCVVNGPGESRHANIGISLPGTGEAPSAPVFIDGEKSVTLRGENI
AYEFIELIDQYVERTYVRRAG

Sequences:

>Translated_421_residues
MHDAVTRPTPPADATAWPRRITRAVKIGSVTVGGGHPVVVQSMTNTDTADIAGSVKQVADLWRAGSEMVRLTVNNAESAA
AIPRIVDKLRMMGIEVPLIGDFHYNGHQLLAAEPACAEALAKYRINPGNVGFGKKKDLQFGQLIEFAIKYGKPVRIGANW
GSLDQSLAAQLMDENSQRDTPWDAGRVLREALIRSAVDSAERAVELGLPRERIILSAKVSGVQELIAVYRDMASRCDFAL
HLGLTEAGIGSKGIVASAAALSVLLQEGIGDTIRISLTPEPGQSRTQEVVVAQELLQTTGQRAFTPMVTACPGCGRTTSE
FFQELAGVVQNHVRAKMPEWKITNPGAENMTLAVMGCVVNGPGESRHANIGISLPGTGEAPSAPVFIDGEKSVTLRGENI
AYEFIELIDQYVERTYVRRAG
>Mature_421_residues
MHDAVTRPTPPADATAWPRRITRAVKIGSVTVGGGHPVVVQSMTNTDTADIAGSVKQVADLWRAGSEMVRLTVNNAESAA
AIPRIVDKLRMMGIEVPLIGDFHYNGHQLLAAEPACAEALAKYRINPGNVGFGKKKDLQFGQLIEFAIKYGKPVRIGANW
GSLDQSLAAQLMDENSQRDTPWDAGRVLREALIRSAVDSAERAVELGLPRERIILSAKVSGVQELIAVYRDMASRCDFAL
HLGLTEAGIGSKGIVASAAALSVLLQEGIGDTIRISLTPEPGQSRTQEVVVAQELLQTTGQRAFTPMVTACPGCGRTTSE
FFQELAGVVQNHVRAKMPEWKITNPGAENMTLAVMGCVVNGPGESRHANIGISLPGTGEAPSAPVFIDGEKSVTLRGENI
AYEFIELIDQYVERTYVRRAG

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=371, Percent_Identity=33.4231805929919, Blast_Score=169, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_XANAC (Q8PLJ8)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_642126.1
- ProteinModelPortal:   Q8PLJ8
- GeneID:   1155870
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC1799
- NMPDR:   fig|190486.1.peg.1770
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- ProtClustDB:   PRK00366
- BioCyc:   XAXO190486:XAC1799-MONOMER
- BRENDA:   1.17.4.3
- HAMAP:   MF_00159
- InterPro:   IPR016425
- InterPro:   IPR004588
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE

EC number: =1.17.7.1

Molecular weight: Translated: 45173; Mature: 45173

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDAVTRPTPPADATAWPRRITRAVKIGSVTVGGGHPVVVQSMTNTDTADIAGSVKQVAD
CCCCCCCCCCCCCCCHHHHHHHHHHEECEEEECCCCCEEEEECCCCCHHHHHHHHHHHHH
LWRAGSEMVRLTVNNAESAAAIPRIVDKLRMMGIEVPLIGDFHYNGHQLLAAEPACAEAL
HHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEECCCHHHHHH
AKYRINPGNVGFGKKKDLQFGQLIEFAIKYGKPVRIGANWGSLDQSLAAQLMDENSQRDT
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCCCCC
PWDAGRVLREALIRSAVDSAERAVELGLPRERIILSAKVSGVQELIAVYRDMASRCDFAL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEHHHHHHHHHHHHHHHHHHHCCEEE
HLGLTEAGIGSKGIVASAAALSVLLQEGIGDTIRISLTPEPGQSRTQEVVVAQELLQTTG
EECCHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCC
QRAFTPMVTACPGCGRTTSEFFQELAGVVQNHVRAKMPEWKITNPGAENMTLAVMGCVVN
CHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCEEEEEEEEEEC
GPGESRHANIGISLPGTGEAPSAPVFIDGEKSVTLRGENIAYEFIELIDQYVERTYVRRA
CCCCCCCCEEEEECCCCCCCCCCCEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCC
G
C
>Mature Secondary Structure
MHDAVTRPTPPADATAWPRRITRAVKIGSVTVGGGHPVVVQSMTNTDTADIAGSVKQVAD
CCCCCCCCCCCCCCCHHHHHHHHHHEECEEEECCCCCEEEEECCCCCHHHHHHHHHHHHH
LWRAGSEMVRLTVNNAESAAAIPRIVDKLRMMGIEVPLIGDFHYNGHQLLAAEPACAEAL
HHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEECCCHHHHHH
AKYRINPGNVGFGKKKDLQFGQLIEFAIKYGKPVRIGANWGSLDQSLAAQLMDENSQRDT
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCCCCC
PWDAGRVLREALIRSAVDSAERAVELGLPRERIILSAKVSGVQELIAVYRDMASRCDFAL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEHHHHHHHHHHHHHHHHHHHCCEEE
HLGLTEAGIGSKGIVASAAALSVLLQEGIGDTIRISLTPEPGQSRTQEVVVAQELLQTTG
EECCHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCC
QRAFTPMVTACPGCGRTTSEFFQELAGVVQNHVRAKMPEWKITNPGAENMTLAVMGCVVN
CHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCEEEEEEEEEEC
GPGESRHANIGISLPGTGEAPSAPVFIDGEKSVTLRGENIAYEFIELIDQYVERTYVRRA
CCCCCCCCEEEEECCCCCCCCCCCEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217