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The map label for this gene is eno

Identifier: 21242468

GI number: 21242468

Start: 1980778

End: 1982070

Strand: Direct

Name: eno

Synonym: XAC1719

Alternate gene names: 21242468

Gene position: 1980778-1982070 (Clockwise)

Preceding gene: 21242467

Following gene: 21242469

Centisome position: 38.27

GC content: 64.97

Gene sequence:

>1293_bases
ATGACCACTATCGCCAAGATCCTCGCCCGCGAAATCCTCGACTCCCGCGGCAATCCCACGCTGGAGGCCGAAGTCACGCT
GGACGACGGTTCGTTCGGCCGCGCGGCTGTGCCGTCGGGTGCCTCGACCGGTACCAAGGAGGCGGTGGAGCTGCGCGATG
GCGACAAGACCCGCTACCTGGGCAAGGGCGTGCGCCATGCGGTGGACAACGTCAACGGCACCATCGCCGAGACGCTGAAG
AACTTCGATGCGGCCGACCAGCAGGGCCTGGACCGTCGCCTGATCGATCTGGACGGCACCGAGAACAAGGGCCGCCTGGG
CGCCAATGCGCTGCTGGGGGTTTCGCTGGCTGCCGCGCATGCCGTGGCAGCCTCGCGCAAGCAGCCGTTGTGGCAGTACC
TGTCCACCATCACCGAGGCCGATGTGGCGCTGCCGGTGCCGATGATGAACATCATCAACGGCGGCGCGCATGCCGACAAC
AACGTCGACTTCCAGGAGTTCATGGTGCTGCCGGTCGGCTGCAGCTCGTTCTCCGAGGCGCTGCGCGCCGGTACCGAAAT
CTTCCATTCGCTCAAGTCGGTGCTCAAGGGCCACGGCCTGAGCACGGCGGTGGGCGACGAAGGCGGCTTTGCGCCGGACT
TCCGCAGCAACGTCGAAGCGCTGGACACCATTCTCGAAGCGATCGGCAAGGCCGGTTACACCGCCGGCGAAGACATCCTG
CTGGGTCTGGACGTGGCGTCCAGCGAGTTCTACGACAACGGCAAGTACAATTTGGTCGGCGAGAACAAGCGCCTGACCAG
TGAGCAGTTCGTCGATTTCCTGGCCGACTGGGTCGCGCAGTACCCGATCATCAGCATCGAAGATGGCCTGGCCGAAGACG
ACTGGGCCGGCTGGAAGCTGCTGACCGATCGCGTCGGCAAGAAGGTGCAGCTGGTGGGCGACGACCTGTTCGTCACCAAC
CCGAAGATCTTCAAGCAGGGCATCGATTCGGGCACCGCCAACGCGATCCTGATCAAGGTCAACCAGATCGGCACGCTGAC
CGAAACGCTGGAAGCCATCGCGATGGCGCACGCGGCCAACTACGCTTCGATCGTGTCGCACCGTTCCGGCGAAACCGAGG
ACACCACCATCGCCGACATCGCCGTGGCCACCACCGCCACGCAGATCAAGACCGGCTCGCTGTGCCGCAGCGACCGCGTG
GCCAAGTACAACCAGCTGCTGCGCATCGAGCAGGCGCTGGGCTCCGGCGCGCGTTACGCCGGCCGCGACGCGTTCGTTTC
GATCAAGCGATAA

Upstream 100 bases:

>100_bases
GTCGCGGCTGCATGCGATTTGGCAATGGACCGGTGTGCGGCGATAATCCGGCAGCTTGTTTCCGGTGTACTTCCCCCCTT
ACTGGTAACCGAACGGACCT

Downstream 100 bases:

>100_bases
GCCGTGCGCAACTGGCGCTGGCTACTGCTGGTGCTGGCGGTACTGCTGGCTTGGCTGCAGTACCGCTTCTGGTTCGGGCC
TGGGAATTCCGGCGAAGTCA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MTTIAKILAREILDSRGNPTLEAEVTLDDGSFGRAAVPSGASTGTKEAVELRDGDKTRYLGKGVRHAVDNVNGTIAETLK
NFDAADQQGLDRRLIDLDGTENKGRLGANALLGVSLAAAHAVAASRKQPLWQYLSTITEADVALPVPMMNIINGGAHADN
NVDFQEFMVLPVGCSSFSEALRAGTEIFHSLKSVLKGHGLSTAVGDEGGFAPDFRSNVEALDTILEAIGKAGYTAGEDIL
LGLDVASSEFYDNGKYNLVGENKRLTSEQFVDFLADWVAQYPIISIEDGLAEDDWAGWKLLTDRVGKKVQLVGDDLFVTN
PKIFKQGIDSGTANAILIKVNQIGTLTETLEAIAMAHAANYASIVSHRSGETEDTTIADIAVATTATQIKTGSLCRSDRV
AKYNQLLRIEQALGSGARYAGRDAFVSIKR

Sequences:

>Translated_430_residues
MTTIAKILAREILDSRGNPTLEAEVTLDDGSFGRAAVPSGASTGTKEAVELRDGDKTRYLGKGVRHAVDNVNGTIAETLK
NFDAADQQGLDRRLIDLDGTENKGRLGANALLGVSLAAAHAVAASRKQPLWQYLSTITEADVALPVPMMNIINGGAHADN
NVDFQEFMVLPVGCSSFSEALRAGTEIFHSLKSVLKGHGLSTAVGDEGGFAPDFRSNVEALDTILEAIGKAGYTAGEDIL
LGLDVASSEFYDNGKYNLVGENKRLTSEQFVDFLADWVAQYPIISIEDGLAEDDWAGWKLLTDRVGKKVQLVGDDLFVTN
PKIFKQGIDSGTANAILIKVNQIGTLTETLEAIAMAHAANYASIVSHRSGETEDTTIADIAVATTATQIKTGSLCRSDRV
AKYNQLLRIEQALGSGARYAGRDAFVSIKR
>Mature_429_residues
TTIAKILAREILDSRGNPTLEAEVTLDDGSFGRAAVPSGASTGTKEAVELRDGDKTRYLGKGVRHAVDNVNGTIAETLKN
FDAADQQGLDRRLIDLDGTENKGRLGANALLGVSLAAAHAVAASRKQPLWQYLSTITEADVALPVPMMNIINGGAHADNN
VDFQEFMVLPVGCSSFSEALRAGTEIFHSLKSVLKGHGLSTAVGDEGGFAPDFRSNVEALDTILEAIGKAGYTAGEDILL
GLDVASSEFYDNGKYNLVGENKRLTSEQFVDFLADWVAQYPIISIEDGLAEDDWAGWKLLTDRVGKKVQLVGDDLFVTNP
KIFKQGIDSGTANAILIKVNQIGTLTETLEAIAMAHAANYASIVSHRSGETEDTTIADIAVATTATQIKTGSLCRSDRVA
KYNQLLRIEQALGSGARYAGRDAFVSIKR

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=431, Percent_Identity=52.9002320185615, Blast_Score=441, Evalue=1e-124,
Organism=Homo sapiens, GI4503571, Length=431, Percent_Identity=50.8120649651972, Blast_Score=437, Evalue=1e-122,
Organism=Homo sapiens, GI301897477, Length=429, Percent_Identity=51.7482517482517, Blast_Score=433, Evalue=1e-121,
Organism=Homo sapiens, GI301897469, Length=429, Percent_Identity=51.7482517482517, Blast_Score=433, Evalue=1e-121,
Organism=Homo sapiens, GI301897479, Length=427, Percent_Identity=47.3067915690867, Blast_Score=376, Evalue=1e-104,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=22.6190476190476, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=22.6190476190476, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=22.6190476190476, Blast_Score=89, Evalue=6e-18,
Organism=Escherichia coli, GI1789141, Length=425, Percent_Identity=62.3529411764706, Blast_Score=527, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI17536383, Length=431, Percent_Identity=52.9002320185615, Blast_Score=432, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI71995829, Length=431, Percent_Identity=52.9002320185615, Blast_Score=431, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI32563855, Length=191, Percent_Identity=49.2146596858639, Blast_Score=190, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6321693, Length=433, Percent_Identity=50.1154734411085, Blast_Score=400, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=47.5750577367206, Blast_Score=381, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=47.5750577367206, Blast_Score=381, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=47.5750577367206, Blast_Score=380, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6321968, Length=433, Percent_Identity=49.1916859122402, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580918, Length=431, Percent_Identity=49.8839907192575, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580916, Length=431, Percent_Identity=49.8839907192575, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580920, Length=431, Percent_Identity=49.8839907192575, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580914, Length=431, Percent_Identity=49.8839907192575, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI281360527, Length=431, Percent_Identity=49.8839907192575, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI17137654, Length=431, Percent_Identity=49.8839907192575, Blast_Score=408, Evalue=1e-114,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_XANAC (Q8PLS0)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_642050.1
- ProteinModelPortal:   Q8PLS0
- SMR:   Q8PLS0
- GeneID:   1155790
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC1719
- NMPDR:   fig|190486.1.peg.1694
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- BioCyc:   XAXO190486:XAC1719-MONOMER
- BRENDA:   4.2.1.11
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45906; Mature: 45775

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 207-207 ACT_SITE 339-339 BINDING 157-157 BINDING 166-166 BINDING 287-287 BINDING 314-314 BINDING 339-339 BINDING 390-390

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIAKILAREILDSRGNPTLEAEVTLDDGSFGRAAVPSGASTGTKEAVELRDGDKTRYL
CCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHEECCCCCHHHH
GKGVRHAVDNVNGTIAETLKNFDAADQQGLDRRLIDLDGTENKGRLGANALLGVSLAAAH
HHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHH
AVAASRKQPLWQYLSTITEADVALPVPMMNIINGGAHADNNVDFQEFMVLPVGCSSFSEA
HHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCHHHHEEEECCHHHHHHH
LRAGTEIFHSLKSVLKGHGLSTAVGDEGGFAPDFRSNVEALDTILEAIGKAGYTAGEDIL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEE
LGLDVASSEFYDNGKYNLVGENKRLTSEQFVDFLADWVAQYPIISIEDGLAEDDWAGWKL
EEEECCCCHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCHHH
LTDRVGKKVQLVGDDLFVTNPKIFKQGIDSGTANAILIKVNQIGTLTETLEAIAMAHAAN
HHHHCCCEEEEECCCEEECCHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
YASIVSHRSGETEDTTIADIAVATTATQIKTGSLCRSDRVAKYNQLLRIEQALGSGARYA
HHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
GRDAFVSIKR
CCCCEEEECC
>Mature Secondary Structure 
TTIAKILAREILDSRGNPTLEAEVTLDDGSFGRAAVPSGASTGTKEAVELRDGDKTRYL
CHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHEECCCCCHHHH
GKGVRHAVDNVNGTIAETLKNFDAADQQGLDRRLIDLDGTENKGRLGANALLGVSLAAAH
HHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHH
AVAASRKQPLWQYLSTITEADVALPVPMMNIINGGAHADNNVDFQEFMVLPVGCSSFSEA
HHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCHHHHEEEECCHHHHHHH
LRAGTEIFHSLKSVLKGHGLSTAVGDEGGFAPDFRSNVEALDTILEAIGKAGYTAGEDIL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEE
LGLDVASSEFYDNGKYNLVGENKRLTSEQFVDFLADWVAQYPIISIEDGLAEDDWAGWKL
EEEECCCCHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCHHH
LTDRVGKKVQLVGDDLFVTNPKIFKQGIDSGTANAILIKVNQIGTLTETLEAIAMAHAAN
HHHHCCCEEEEECCCEEECCHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
YASIVSHRSGETEDTTIADIAVATTATQIKTGSLCRSDRVAKYNQLLRIEQALGSGARYA
HHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
GRDAFVSIKR
CCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217