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The map label for this gene is grpE

Identifier: 21242272

GI number: 21242272

Start: 1754577

End: 1755095

Strand: Direct

Name: grpE

Synonym: XAC1521

Alternate gene names: 21242272

Gene position: 1754577-1755095 (Clockwise)

Preceding gene: 21242271

Following gene: 21242273

Centisome position: 33.9

GC content: 66.67

Gene sequence:

>519_bases
ATGAACCAAGACCACCCCGAATTCGACTCCGAAGACCTGTCCCAGAATCCACCAGAGACCGATCCGCTCAAGGCCGAGAT
CGAATCGCTGCGCAGCGAGATCGCCCTGGTCAAGGCCGACGCCCTGCGGGAGCGCGCCGACCTGGAAAACCAGCGCAAGC
GCATCGCCCGCGACGTGGAAAACGCGCGCAAGTTCGCCAACGAGAAGCTGCTCGGCGAGCTGCTGCCGGTGTTCGATAGC
CTGGACGCGGGACTCACCGCCGCCGGTACCGAGCCCAGCCCGCTGCGCGATGGCCTGGACATGACCTACAAGCAATTGCT
CAAGGTGGCCGCGGACAACGGCCTGACCCTGCTCGATCCGGTCGGCCAGCCGTTCAACCCGGACCAGCACCAGGCGATCA
GCCAGGGCGAGGCCGAGGGCGTCGCGCCGGGCCATGTGGTGCAGGTGTTCCAGAAGGGCTACCTGCTCAACGACCGCCTG
CTGCGCCCGGCCCTGGTGGTCGTGGCCAAGCATGACTGA

Upstream 100 bases:

>100_bases
CCGGCATCGATCGGCGCGATCGTGTTTGCTTGAAACGCGCGCAGGCGCCCATATACGGGGTGACGTTGGGGCGGGCGTTC
CGCTTGCCCGGGAACTGCAC

Downstream 100 bases:

>100_bases
ACCGCAGCGCCGGCTGAACTGCCAGGCGCAGGCATGAAACACAGATGTGCGCCGGTGGCTTGAACACCTGCGCACCACCC
CCACATCTCAGCCAGACCCG

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MNQDHPEFDSEDLSQNPPETDPLKAEIESLRSEIALVKADALRERADLENQRKRIARDVENARKFANEKLLGELLPVFDS
LDAGLTAAGTEPSPLRDGLDMTYKQLLKVAADNGLTLLDPVGQPFNPDQHQAISQGEAEGVAPGHVVQVFQKGYLLNDRL
LRPALVVVAKHD

Sequences:

>Translated_172_residues
MNQDHPEFDSEDLSQNPPETDPLKAEIESLRSEIALVKADALRERADLENQRKRIARDVENARKFANEKLLGELLPVFDS
LDAGLTAAGTEPSPLRDGLDMTYKQLLKVAADNGLTLLDPVGQPFNPDQHQAISQGEAEGVAPGHVVQVFQKGYLLNDRL
LRPALVVVAKHD
>Mature_172_residues
MNQDHPEFDSEDLSQNPPETDPLKAEIESLRSEIALVKADALRERADLENQRKRIARDVENARKFANEKLLGELLPVFDS
LDAGLTAAGTEPSPLRDGLDMTYKQLLKVAADNGLTLLDPVGQPFNPDQHQAISQGEAEGVAPGHVVQVFQKGYLLNDRL
LRPALVVVAKHD

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=176, Percent_Identity=34.6590909090909, Blast_Score=80, Evalue=7e-16,
Organism=Homo sapiens, GI40255109, Length=173, Percent_Identity=32.3699421965318, Blast_Score=67, Evalue=5e-12,
Organism=Escherichia coli, GI1788967, Length=173, Percent_Identity=36.9942196531792, Blast_Score=114, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17552458, Length=172, Percent_Identity=33.1395348837209, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324806, Length=169, Percent_Identity=31.3609467455621, Blast_Score=85, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24653432, Length=164, Percent_Identity=34.7560975609756, Blast_Score=95, Evalue=2e-20,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_XANAC (Q8PMB1)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_641854.1
- ProteinModelPortal:   Q8PMB1
- SMR:   Q8PMB1
- GeneID:   1155592
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC1521
- HOGENOM:   HBG732630
- OMA:   EVEPNSV
- ProtClustDB:   PRK14147
- BioCyc:   XAXO190486:XAC1521-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 18938; Mature: 18938

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQDHPEFDSEDLSQNPPETDPLKAEIESLRSEIALVKADALRERADLENQRKRIARDVE
CCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NARKFANEKLLGELLPVFDSLDAGLTAAGTEPSPLRDGLDMTYKQLLKVAADNGLTLLDP
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECC
VGQPFNPDQHQAISQGEAEGVAPGHVVQVFQKGYLLNDRLLRPALVVVAKHD
CCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHCCHHHEEECCC
>Mature Secondary Structure
MNQDHPEFDSEDLSQNPPETDPLKAEIESLRSEIALVKADALRERADLENQRKRIARDVE
CCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NARKFANEKLLGELLPVFDSLDAGLTAAGTEPSPLRDGLDMTYKQLLKVAADNGLTLLDP
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECC
VGQPFNPDQHQAISQGEAEGVAPGHVVQVFQKGYLLNDRLLRPALVVVAKHD
CCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHCCHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217