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The map label for this gene is oprN [H]

Identifier: 21242237

GI number: 21242237

Start: 1716831

End: 1718255

Strand: Direct

Name: oprN [H]

Synonym: XAC1485

Alternate gene names: 21242237

Gene position: 1716831-1718255 (Clockwise)

Preceding gene: 21242236

Following gene: 21242239

Centisome position: 33.17

GC content: 67.58

Gene sequence:

>1425_bases
ATGGTAAGAAACGTCCTGAGTGTGGCCCTGGCCGCGCTTGTGCTCAGCGGTTGCGCGGTCGGGCTGGATTACCGTCCCGA
TCCGCCGGCGGCGGTGACGCTGCAGGGCGCGCAAGACCCGGCATACAGCGCACAGTCGCCGGTGGGCAACTGGTGGTCGC
AGTTCGACGACCCGGTGCTGGAGCAGTTGGTGCGCGACGCCTTGTTCGCCAACCACGACCTGCGCATCGCCGTGGCGCGC
GTCAAGCAGGCGCGCGCGGTGTTCGTCGAGCGTCGTCTGGATCAGGCGCCGCATATCACTGCGCAGGGCAGCTTCGATCG
CCGCGAACAGCAGCAGGTGATCGCCGGCAATCAGCGCTTTCTGACCGAGCAGACCACGCTCGGTCTGGATGCGGCCTGGG
AGCTGGACCTGTTCGGCCGTCAGCGGCGTGCATCGGAGGCCGCGCGTGCCGACCTGGATGCCGAGCAGGCCAACCTACAG
GACGTGCAGGTCACCGTTGCTGCCGAAGTGGCGCGCAACTATTTCGAACTGCGCGGTACCCAGAAGCGGCTGGATGTCTC
CAAGCGCACGCTCACCAACCTGCGCGACACCCAGAAGCTGACGCAGACGCGTTGGGATCTGGGGGCCGGCAGCGAGCTGG
ATGTGCAGAGCAGCCTTGCGCGGTTGAAGGCGATCGAAGCGGATATTCCGCTGCTGGAAACCGCCGAGGCGCAATATCGC
CATCGTCTGGCTGTGTTGCTGGGTCAGCAACCCGATGCCTTGGATGCCACCTTGGCCGCGCGCGAGATGCCGGCGTTCGC
GCGCCCGCTGCCGCTGGGCGATACCGCCGGCTTGCTGCGTCAGCGTCCGGATGTGCGCGGCGCCGAGCGCCGGCTGGCGG
CAGCCACCGCGCAAGTGGGCGTGGCCACTGCCGACCTGTTTCCGCGCATCAGCGTGAGCGGGTTCGTCGGCTTCCTGACC
GGCGACGCGGCCAGGTTGACCGAAGGCAACGCCAAGGCCTGGTCGGTCACCCCGTCGATCAGCTGGGCCGCCTTCGATTT
CGGCACCGTGCGTGCGCGCTTGCGTGCCAGCAAGGCGCAGGCCGAAGGCGCGTTGGCGCAGTATCAGCAAACCGTACTGC
TGGCGCTGGAAGACACCGAAAACGCGTTGATCGGCTATGGCCGCCAGCAGGCGCGTCTGGCCATCGTGGTGGAGCAGGCC
AATGCCGCGCGGCGTGCCGAGCAGCTGGCGCAGATCCGCTACCGCGAGGGCTCGGAAGACTTCCTCACCCTGCTGGATGC
GCAGCGCACCCAACTGGCTGCCGACGATGCGTTGGCGCAGGCCGAAACGGCAGTGAATGTCGGCGTGGTCGGCGTGTACA
AGGCGCTCGGTGGTTGGAAGCAGGACCAAGCACCCGCTGCACCGGTGGCCGTGGTCAATCGTTGA

Upstream 100 bases:

>100_bases
AAATCGAAGTGGAGCAGGGTGCGCATAGCAGCGTGCAGATGGCGTTGTTGGATGCACACGGCGCCACCGGCAGCTTTACC
CATCTGGGAGAGGTGTTGCC

Downstream 100 bases:

>100_bases
GCGATGCGTGCGGGGCGGCCAGTGGGTCGTCCCGCAGTTCTGGCTGCAGCGTCAGAGCCAAGCGGGGCGGTCGAACGTGT
GCGGCTGATGGCTGGCGATG

Product: outer membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MVRNVLSVALAALVLSGCAVGLDYRPDPPAAVTLQGAQDPAYSAQSPVGNWWSQFDDPVLEQLVRDALFANHDLRIAVAR
VKQARAVFVERRLDQAPHITAQGSFDRREQQQVIAGNQRFLTEQTTLGLDAAWELDLFGRQRRASEAARADLDAEQANLQ
DVQVTVAAEVARNYFELRGTQKRLDVSKRTLTNLRDTQKLTQTRWDLGAGSELDVQSSLARLKAIEADIPLLETAEAQYR
HRLAVLLGQQPDALDATLAAREMPAFARPLPLGDTAGLLRQRPDVRGAERRLAAATAQVGVATADLFPRISVSGFVGFLT
GDAARLTEGNAKAWSVTPSISWAAFDFGTVRARLRASKAQAEGALAQYQQTVLLALEDTENALIGYGRQQARLAIVVEQA
NAARRAEQLAQIRYREGSEDFLTLLDAQRTQLAADDALAQAETAVNVGVVGVYKALGGWKQDQAPAAPVAVVNR

Sequences:

>Translated_474_residues
MVRNVLSVALAALVLSGCAVGLDYRPDPPAAVTLQGAQDPAYSAQSPVGNWWSQFDDPVLEQLVRDALFANHDLRIAVAR
VKQARAVFVERRLDQAPHITAQGSFDRREQQQVIAGNQRFLTEQTTLGLDAAWELDLFGRQRRASEAARADLDAEQANLQ
DVQVTVAAEVARNYFELRGTQKRLDVSKRTLTNLRDTQKLTQTRWDLGAGSELDVQSSLARLKAIEADIPLLETAEAQYR
HRLAVLLGQQPDALDATLAAREMPAFARPLPLGDTAGLLRQRPDVRGAERRLAAATAQVGVATADLFPRISVSGFVGFLT
GDAARLTEGNAKAWSVTPSISWAAFDFGTVRARLRASKAQAEGALAQYQQTVLLALEDTENALIGYGRQQARLAIVVEQA
NAARRAEQLAQIRYREGSEDFLTLLDAQRTQLAADDALAQAETAVNVGVVGVYKALGGWKQDQAPAAPVAVVNR
>Mature_474_residues
MVRNVLSVALAALVLSGCAVGLDYRPDPPAAVTLQGAQDPAYSAQSPVGNWWSQFDDPVLEQLVRDALFANHDLRIAVAR
VKQARAVFVERRLDQAPHITAQGSFDRREQQQVIAGNQRFLTEQTTLGLDAAWELDLFGRQRRASEAARADLDAEQANLQ
DVQVTVAAEVARNYFELRGTQKRLDVSKRTLTNLRDTQKLTQTRWDLGAGSELDVQSSLARLKAIEADIPLLETAEAQYR
HRLAVLLGQQPDALDATLAAREMPAFARPLPLGDTAGLLRQRPDVRGAERRLAAATAQVGVATADLFPRISVSGFVGFLT
GDAARLTEGNAKAWSVTPSISWAAFDFGTVRARLRASKAQAEGALAQYQQTVLLALEDTENALIGYGRQQARLAIVVEQA
NAARRAEQLAQIRYREGSEDFLTLLDAQRTQLAADDALAQAETAVNVGVVGVYKALGGWKQDQAPAAPVAVVNR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=453, Percent_Identity=28.2560706401766, Blast_Score=158, Evalue=7e-40,
Organism=Escherichia coli, GI2367348, Length=500, Percent_Identity=24.6, Blast_Score=92, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 51503; Mature: 51503

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRNVLSVALAALVLSGCAVGLDYRPDPPAAVTLQGAQDPAYSAQSPVGNWWSQFDDPVL
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHCCCHHH
EQLVRDALFANHDLRIAVARVKQARAVFVERRLDQAPHITAQGSFDRREQQQVIAGNQRF
HHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHCCCCCE
LTEQTTLGLDAAWELDLFGRQRRASEAARADLDAEQANLQDVQVTVAAEVARNYFELRGT
EEHHHHCCCCCEEEEHHHCCHHHHHHHHHHCCCHHHCCCCCEEEHHHHHHHHHHHHHCCC
QKRLDVSKRTLTNLRDTQKLTQTRWDLGAGSELDVQSSLARLKAIEADIPLLETAEAQYR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
HRLAVLLGQQPDALDATLAAREMPAFARPLPLGDTAGLLRQRPDVRGAERRLAAATAQVG
HHHHHHCCCCCCHHHHHHHHHHCCHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHC
VATADLFPRISVSGFVGFLTGDAARLTEGNAKAWSVTPSISWAAFDFGTVRARLRASKAQ
HHHHHHCCCCCHHHEEEEEECCCHHCCCCCCCEEEECCCCCEEEECHHHHHHHHHHHHHH
AEGALAQYQQTVLLALEDTENALIGYGRQQARLAIVVEQANAARRAEQLAQIRYREGSED
HHHHHHHHHCEEEEEEECCCCCEECCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCHH
FLTLLDAQRTQLAADDALAQAETAVNVGVVGVYKALGGWKQDQAPAAPVAVVNR
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCEEECCC
>Mature Secondary Structure
MVRNVLSVALAALVLSGCAVGLDYRPDPPAAVTLQGAQDPAYSAQSPVGNWWSQFDDPVL
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHCCCHHH
EQLVRDALFANHDLRIAVARVKQARAVFVERRLDQAPHITAQGSFDRREQQQVIAGNQRF
HHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHCCCCCE
LTEQTTLGLDAAWELDLFGRQRRASEAARADLDAEQANLQDVQVTVAAEVARNYFELRGT
EEHHHHCCCCCEEEEHHHCCHHHHHHHHHHCCCHHHCCCCCEEEHHHHHHHHHHHHHCCC
QKRLDVSKRTLTNLRDTQKLTQTRWDLGAGSELDVQSSLARLKAIEADIPLLETAEAQYR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
HRLAVLLGQQPDALDATLAAREMPAFARPLPLGDTAGLLRQRPDVRGAERRLAAATAQVG
HHHHHHCCCCCCHHHHHHHHHHCCHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHC
VATADLFPRISVSGFVGFLTGDAARLTEGNAKAWSVTPSISWAAFDFGTVRARLRASKAQ
HHHHHHCCCCCHHHEEEEEECCCHHCCCCCCCEEEECCCCCEEEECHHHHHHHHHHHHHH
AEGALAQYQQTVLLALEDTENALIGYGRQQARLAIVVEQANAARRAEQLAQIRYREGSED
HHHHHHHHHCEEEEEEECCCCCEECCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCHH
FLTLLDAQRTQLAADDALAQAETAVNVGVVGVYKALGGWKQDQAPAAPVAVVNR
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]