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The map label for this gene is dnaB [H]

Identifier: 21242229

GI number: 21242229

Start: 1704558

End: 1705988

Strand: Direct

Name: dnaB [H]

Synonym: XAC1477

Alternate gene names: 21242229

Gene position: 1704558-1705988 (Clockwise)

Preceding gene: 21242228

Following gene: 21242230

Centisome position: 32.93

GC content: 62.96

Gene sequence:

>1431_bases
ATGTCCGCTCGTCCTGGTTTCCGTTCCAAGCGCAGTCGCGATCGCGACGACGACGATTACGATCGTCCCGAGCCGCGTCT
GGACCAGCTGCGGGTACCGCCGCATTCGGTCGAGGCCGAGCAGGCGGTGCTGGGTGGCTTGATGCTGGCGCCGGACGCGT
TCGACCGGGTCAACGACCAGCTCACCGAAAACGACTTTTACCGGCGCGACCATCGGCTGATCTACCGCGCGATTCGCGAA
CTCAACGAGAAAGACCGCCCGTTCGATGCAGTGACCCTGGGCGAATGGTTCGAGTCGCAGGGCAAGCTGGAGCAGGTGGG
CGATGGCGCGTACCTGATCGAGCTGGCCAGCACCACGCCGTCGGCGGCCAACATCGCCGCGTATGCGGAAATCGTGCGCG
ACAAGGCGGTGCTGCGGCAGTTGATCGAAGTGGGCACCACCATCGTCAACGACGGCTTTCAGCCGGAAGGGCGCGACAGC
GTCGAGTTGCTGTCCTCGGCCGAAAAGGCCGTGTTCAAGATCGCCGAAGCCGGCGCGCGTGGGCGCACCGATTTCGTGGC
CATGCCCGGGGCATTGAAGGATGCCTTCGAAGAGCTGCGCAACCGCTTCGAAAACGGCGGCAACATCACGGGTCTGCCGA
CCGGCTACACCGATTTCGATGCGATGACCGCCGGCCTGCAGCCGACCGACCTGATCATCCTGGCGGCGCGTCCGGCGATG
GGCAAAACCACCTTTGCGCTGAACATCGCCGAATATGCGGCGATCAAGTCCAAGAAGGGCGTGGCGGTGTTTTCGATGGA
AATGTCGGCCTCGCAGTTGGCCATGCGCCTGATCTCCTCCAATGGCCGCATCAATGCGCAGCGCCTGCGTACCGGTGCGC
TGGAAGACGAGGATTGGGCGCGCGTGACCGGCGCGATCAAGATGCTCAAGGAAACCAAGATCTTCATCGACGACACACCT
GGCGTGTCGCCGGAAGTACTGCGTTCCAAGTGCCGCCGGCTCAAGCGCGAGCACGACCTGGGCCTGATCGTCATCGACTA
CCTGCAGCTGATGTCGGTGCCGGGCAACAGCGAAAACCGCGCCACCGAAATTTCGGAAATCTCGCGCTCGCTGAAAGGCT
TGGCCAAGGAGCTCAACGTGCCGGTGATTGCGTTGTCCCAGCTCAACCGCTCGCTGGAAACGCGCACCGACAAGCGCCCG
GTGATGGCGGACTTGCGCGAGTCGGGCGCGATCGAGCAGGACGCGGACATGATCGTCTTCATCTACCGCGACGACTACTA
CAACAAGGAAAATTCGCCGGACAAGGGCCTGGCCGAAATCATCATCGGCAAGCACCGCGGCGGACCGACCGGCTCGTGCA
AGCTGAAGTTCTTCGGCGAATACACCCGGTTCGACAACCTGGCGCACGACTCGGTGGGCAGTTTCGAGTAA

Upstream 100 bases:

>100_bases
CTGGCTATACACCAGTTATCCACAGAGTTGTGCATGGTCGCCGGCTGCGCCGATGACTATGCTTCCTGCCCTCGTCTCCT
CGCGTCAGGTTTGTTTGTCC

Downstream 100 bases:

>100_bases
GCGCTGTCGTGGAACGGCCGGGTGGTCATCCGCCGCCCGGCATCGCGCCATCGTGGCGCCCGCTGGCTTATGCTGCGCAG
CCTCATTGTTTGCTGCCGAG

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MSARPGFRSKRSRDRDDDDYDRPEPRLDQLRVPPHSVEAEQAVLGGLMLAPDAFDRVNDQLTENDFYRRDHRLIYRAIRE
LNEKDRPFDAVTLGEWFESQGKLEQVGDGAYLIELASTTPSAANIAAYAEIVRDKAVLRQLIEVGTTIVNDGFQPEGRDS
VELLSSAEKAVFKIAEAGARGRTDFVAMPGALKDAFEELRNRFENGGNITGLPTGYTDFDAMTAGLQPTDLIILAARPAM
GKTTFALNIAEYAAIKSKKGVAVFSMEMSASQLAMRLISSNGRINAQRLRTGALEDEDWARVTGAIKMLKETKIFIDDTP
GVSPEVLRSKCRRLKREHDLGLIVIDYLQLMSVPGNSENRATEISEISRSLKGLAKELNVPVIALSQLNRSLETRTDKRP
VMADLRESGAIEQDADMIVFIYRDDYYNKENSPDKGLAEIIIGKHRGGPTGSCKLKFFGEYTRFDNLAHDSVGSFE

Sequences:

>Translated_476_residues
MSARPGFRSKRSRDRDDDDYDRPEPRLDQLRVPPHSVEAEQAVLGGLMLAPDAFDRVNDQLTENDFYRRDHRLIYRAIRE
LNEKDRPFDAVTLGEWFESQGKLEQVGDGAYLIELASTTPSAANIAAYAEIVRDKAVLRQLIEVGTTIVNDGFQPEGRDS
VELLSSAEKAVFKIAEAGARGRTDFVAMPGALKDAFEELRNRFENGGNITGLPTGYTDFDAMTAGLQPTDLIILAARPAM
GKTTFALNIAEYAAIKSKKGVAVFSMEMSASQLAMRLISSNGRINAQRLRTGALEDEDWARVTGAIKMLKETKIFIDDTP
GVSPEVLRSKCRRLKREHDLGLIVIDYLQLMSVPGNSENRATEISEISRSLKGLAKELNVPVIALSQLNRSLETRTDKRP
VMADLRESGAIEQDADMIVFIYRDDYYNKENSPDKGLAEIIIGKHRGGPTGSCKLKFFGEYTRFDNLAHDSVGSFE
>Mature_475_residues
SARPGFRSKRSRDRDDDDYDRPEPRLDQLRVPPHSVEAEQAVLGGLMLAPDAFDRVNDQLTENDFYRRDHRLIYRAIREL
NEKDRPFDAVTLGEWFESQGKLEQVGDGAYLIELASTTPSAANIAAYAEIVRDKAVLRQLIEVGTTIVNDGFQPEGRDSV
ELLSSAEKAVFKIAEAGARGRTDFVAMPGALKDAFEELRNRFENGGNITGLPTGYTDFDAMTAGLQPTDLIILAARPAMG
KTTFALNIAEYAAIKSKKGVAVFSMEMSASQLAMRLISSNGRINAQRLRTGALEDEDWARVTGAIKMLKETKIFIDDTPG
VSPEVLRSKCRRLKREHDLGLIVIDYLQLMSVPGNSENRATEISEISRSLKGLAKELNVPVIALSQLNRSLETRTDKRPV
MADLRESGAIEQDADMIVFIYRDDYYNKENSPDKGLAEIIIGKHRGGPTGSCKLKFFGEYTRFDNLAHDSVGSFE

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=473, Percent_Identity=52.8541226215645, Blast_Score=478, Evalue=1e-136,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 52918; Mature: 52787

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARPGFRSKRSRDRDDDDYDRPEPRLDQLRVPPHSVEAEQAVLGGLMLAPDAFDRVNDQ
CCCCCCCCCHHCCCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHCCHHCCCHHHHHHHHH
LTENDFYRRDHRLIYRAIRELNEKDRPFDAVTLGEWFESQGKLEQVGDGAYLIELASTTP
HHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHCCCCHHHCCCCEEEEEEECCCC
SAANIAAYAEIVRDKAVLRQLIEVGTTIVNDGFQPEGRDSVELLSSAEKAVFKIAEAGAR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
GRTDFVAMPGALKDAFEELRNRFENGGNITGLPTGYTDFDAMTAGLQPTDLIILAARPAM
CCCCEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCEEEEEECCCC
GKTTFALNIAEYAAIKSKKGVAVFSMEMSASQLAMRLISSNGRINAQRLRTGALEDEDWA
CCCEEEEEHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCEEHHHHHCCCCCCHHHH
RVTGAIKMLKETKIFIDDTPGVSPEVLRSKCRRLKREHDLGLIVIDYLQLMSVPGNSENR
HHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCH
ATEISEISRSLKGLAKELNVPVIALSQLNRSLETRTDKRPVMADLRESGAIEQDADMIVF
HHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEE
IYRDDYYNKENSPDKGLAEIIIGKHRGGPTGSCKLKFFGEYTRFDNLAHDSVGSFE
EEECCCCCCCCCCCCCHHHHHEECCCCCCCCCEEEEEEECHHHHCCHHHCCCCCCC
>Mature Secondary Structure 
SARPGFRSKRSRDRDDDDYDRPEPRLDQLRVPPHSVEAEQAVLGGLMLAPDAFDRVNDQ
CCCCCCCCHHCCCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHCCHHCCCHHHHHHHHH
LTENDFYRRDHRLIYRAIRELNEKDRPFDAVTLGEWFESQGKLEQVGDGAYLIELASTTP
HHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHCCCCHHHCCCCEEEEEEECCCC
SAANIAAYAEIVRDKAVLRQLIEVGTTIVNDGFQPEGRDSVELLSSAEKAVFKIAEAGAR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
GRTDFVAMPGALKDAFEELRNRFENGGNITGLPTGYTDFDAMTAGLQPTDLIILAARPAM
CCCCEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCEEEEEECCCC
GKTTFALNIAEYAAIKSKKGVAVFSMEMSASQLAMRLISSNGRINAQRLRTGALEDEDWA
CCCEEEEEHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCEEHHHHHCCCCCCHHHH
RVTGAIKMLKETKIFIDDTPGVSPEVLRSKCRRLKREHDLGLIVIDYLQLMSVPGNSENR
HHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCH
ATEISEISRSLKGLAKELNVPVIALSQLNRSLETRTDKRPVMADLRESGAIEQDADMIVF
HHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEE
IYRDDYYNKENSPDKGLAEIIIGKHRGGPTGSCKLKFFGEYTRFDNLAHDSVGSFE
EEECCCCCCCCCCCCCHHHHHEECCCCCCCCCEEEEEEECHHHHCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]