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The map label for this gene is asnB [H]

Identifier: 21242227

GI number: 21242227

Start: 1701670

End: 1703610

Strand: Direct

Name: asnB [H]

Synonym: XAC1475

Alternate gene names: 21242227

Gene position: 1701670-1703610 (Clockwise)

Preceding gene: 77748589

Following gene: 21242228

Centisome position: 32.88

GC content: 68.26

Gene sequence:

>1941_bases
ATGTGCGGATTGGCAGGAATGTTGCTGGCCTCGCCGCGCCTGCATGGCGAACAGCTCGATGCGCTGGTGCGGCCGATGGG
GGCCGCCTTGCGCCACCGCGGCCCCGACGATGCCGGCACCTGGTGCGATGCGCAGGCCGGCGTAGCGCTGGCGCATCAGC
GCTTGAGCATTCTGGATCTGTCGCCGCTCGGCCATCAGCCGATGCGCTCGGCTGACGGCCGCTACGTGCTGGCCTATAAC
GGCGAGGTCTATAACTTCGCGCAATTGCGCGCAGCGCTTTCCGCGCTTGGGCATCGCTTTCGCGGCCATTCCGATACTGA
GGTGCTGCTGGCGGCCGTGGTGGAGTGGGGGCTGGACGAGACGTTGACCCGCTGCAACGGCATGTTCGCCATCGCGTTGT
GGGACGAGCGCGACAACTGCCTGTTCCTGGCCCGCGACCGCGTCGGCAAGAAGCCGCTGTACTACGGGTGGGCCGGCGAC
ACCTTGGTGTTCGGTTCCGAACTCAAGGCCTTGTGGCAGCACCCGGATTTCGACAACGGCGTGGATCGCGATGCGCTCAC
CCTGCTGCTGCGGCTTGGCTATATCCCGGCGCCGGCCTGCATCCACGAGCACACCTTCAAGCTGATGCCCGGCCGCGTGC
TGCGCATCGATGCACAAGCCGTCGCAGCCGGCGCGGCCGCGCATCGTCCCGATCAGGCGCAGCAACCGTTCTGGAACGCG
CGCGAAGCGATGCAACGCGCGCTGGCGACGCCGTTCACCGGTACCGACGCGCAGGCCGAAGAGCAATTGGATCGCGTGCT
GCGCGATGCGGTGGCCTTGCGCATGGTGGCCGATGTGCCGGTTGGCGTGTTTCTCTCCGGCGGCACCGACTCGTCCATCG
TCACTGCGATGATGCAGGCGCAGAGCGCGCAGCCGGTGCACACCTTCAGCATCGGCTTCGAAGGTTCGCATCACGACGAA
GCGCCGCTGGCGCGCGAAGTGGCCACGCATCTGTGCACCGACCACACCGAACTGTATGTCAGCGGCGCCGATGCGCTGGC
GGTGGTGCCGAACCTGCCGGACATGTTCGACGAACCCTTCGCCGATGCGTCGCAAGTGCCGACGGCGTTGGTCGCACGGC
TGGCGCGCGGCGGAGTCACGGTGGCGCTGTCCGGCGATGGTGGCGACGAATTGTTCTTCGGCTATGGTCGGTATCAGCGC
GCACTGCGCAATTGGCGCATGCACGGCCTGGTGCCGGGCCCGCTGCGGCGGTTGATGGCGGTGGCGGCGCGCCGCAACGG
CGAGTCCTCGCGCACCGGCGGCCTGGCCGCGCTGGTGGCCGAGGCCGGCGCACGCGGCATCGGCGATATCTACCGCAACC
GCATTTCGCGCTGGCGCGATCCGGCCGCGGTGGTGCTGGGCGCCACCGAGCCCGACAGTTTCTACAGCCTGGCCGACCCG
CTGCATGGGTCCGGTACGCCGGCCGACGCGATGATGCTGGCCGATTTTGCCGCCTACTTGCCGGACGATCTGCTGTGCAA
GGTGGACCGCACCACCATGGCGGTGGGGCTGGAAGCGCGTGCGCCGCTGCTGGATTGGCGCGTGGCCGAATTCGCCTGGT
CGCTGCCGCTGTCGCTGAAGTATCGCGATGGAGTGAGCAAGTATCTGCTCAAGCGCGTGCTGTGCCGCTACCTGCCGGAC
GCGATGGTCTACCGCGGCAAGCGCGGCTTCGGCGCACCGGTCAGCGCATGGCTGCGCGGCGATCTGCACGGCTGGGCAGA
CGATCTGCTGGCGCACGGCGCCCTGCAGCGCGAGGGCGTATTCGCCGCAGACAGCGTGGCCCGCCTGTGGCGCGACTTCA
ACGGTGGCGAGCGCAAATGGCATACGCATCTATGGACGGTGCTGATGTTCCAGGCCTGGCAGGCACACTGGCGCGAACAG
CGGGCGGCCGTGGTGCGCTGA

Upstream 100 bases:

>100_bases
CGGCATCGGCATTCGCCGTGCGCCTGTTCGGGCGCGTACGACGCGTCGTGCGTGCCGGCGCCGCCCGGTTAGTCGCGGCC
GGCCGGCAGGAGCGCTGACG

Downstream 100 bases:

>100_bases
CGCCTGGCGCTGCGGTGTGGACGCTGGTCGGCAGTGGGCGAAGCCTCCTGCGAACGTTGCCGCGCTCGCGTGTACGCACC
GCCGCTTGCGCCACAGATGC

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 646; Mature: 646

Protein sequence:

>646_residues
MCGLAGMLLASPRLHGEQLDALVRPMGAALRHRGPDDAGTWCDAQAGVALAHQRLSILDLSPLGHQPMRSADGRYVLAYN
GEVYNFAQLRAALSALGHRFRGHSDTEVLLAAVVEWGLDETLTRCNGMFAIALWDERDNCLFLARDRVGKKPLYYGWAGD
TLVFGSELKALWQHPDFDNGVDRDALTLLLRLGYIPAPACIHEHTFKLMPGRVLRIDAQAVAAGAAAHRPDQAQQPFWNA
REAMQRALATPFTGTDAQAEEQLDRVLRDAVALRMVADVPVGVFLSGGTDSSIVTAMMQAQSAQPVHTFSIGFEGSHHDE
APLAREVATHLCTDHTELYVSGADALAVVPNLPDMFDEPFADASQVPTALVARLARGGVTVALSGDGGDELFFGYGRYQR
ALRNWRMHGLVPGPLRRLMAVAARRNGESSRTGGLAALVAEAGARGIGDIYRNRISRWRDPAAVVLGATEPDSFYSLADP
LHGSGTPADAMMLADFAAYLPDDLLCKVDRTTMAVGLEARAPLLDWRVAEFAWSLPLSLKYRDGVSKYLLKRVLCRYLPD
AMVYRGKRGFGAPVSAWLRGDLHGWADDLLAHGALQREGVFAADSVARLWRDFNGGERKWHTHLWTVLMFQAWQAHWREQ
RAAVVR

Sequences:

>Translated_646_residues
MCGLAGMLLASPRLHGEQLDALVRPMGAALRHRGPDDAGTWCDAQAGVALAHQRLSILDLSPLGHQPMRSADGRYVLAYN
GEVYNFAQLRAALSALGHRFRGHSDTEVLLAAVVEWGLDETLTRCNGMFAIALWDERDNCLFLARDRVGKKPLYYGWAGD
TLVFGSELKALWQHPDFDNGVDRDALTLLLRLGYIPAPACIHEHTFKLMPGRVLRIDAQAVAAGAAAHRPDQAQQPFWNA
REAMQRALATPFTGTDAQAEEQLDRVLRDAVALRMVADVPVGVFLSGGTDSSIVTAMMQAQSAQPVHTFSIGFEGSHHDE
APLAREVATHLCTDHTELYVSGADALAVVPNLPDMFDEPFADASQVPTALVARLARGGVTVALSGDGGDELFFGYGRYQR
ALRNWRMHGLVPGPLRRLMAVAARRNGESSRTGGLAALVAEAGARGIGDIYRNRISRWRDPAAVVLGATEPDSFYSLADP
LHGSGTPADAMMLADFAAYLPDDLLCKVDRTTMAVGLEARAPLLDWRVAEFAWSLPLSLKYRDGVSKYLLKRVLCRYLPD
AMVYRGKRGFGAPVSAWLRGDLHGWADDLLAHGALQREGVFAADSVARLWRDFNGGERKWHTHLWTVLMFQAWQAHWREQ
RAAVVR
>Mature_646_residues
MCGLAGMLLASPRLHGEQLDALVRPMGAALRHRGPDDAGTWCDAQAGVALAHQRLSILDLSPLGHQPMRSADGRYVLAYN
GEVYNFAQLRAALSALGHRFRGHSDTEVLLAAVVEWGLDETLTRCNGMFAIALWDERDNCLFLARDRVGKKPLYYGWAGD
TLVFGSELKALWQHPDFDNGVDRDALTLLLRLGYIPAPACIHEHTFKLMPGRVLRIDAQAVAAGAAAHRPDQAQQPFWNA
REAMQRALATPFTGTDAQAEEQLDRVLRDAVALRMVADVPVGVFLSGGTDSSIVTAMMQAQSAQPVHTFSIGFEGSHHDE
APLAREVATHLCTDHTELYVSGADALAVVPNLPDMFDEPFADASQVPTALVARLARGGVTVALSGDGGDELFFGYGRYQR
ALRNWRMHGLVPGPLRRLMAVAARRNGESSRTGGLAALVAEAGARGIGDIYRNRISRWRDPAAVVLGATEPDSFYSLADP
LHGSGTPADAMMLADFAAYLPDDLLCKVDRTTMAVGLEARAPLLDWRVAEFAWSLPLSLKYRDGVSKYLLKRVLCRYLPD
AMVYRGKRGFGAPVSAWLRGDLHGWADDLLAHGALQREGVFAADSVARLWRDFNGGERKWHTHLWTVLMFQAWQAHWREQ
RAAVVR

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786889, Length=439, Percent_Identity=30.751708428246, Blast_Score=151, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI71993933, Length=408, Percent_Identity=26.2254901960784, Blast_Score=120, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI25147557, Length=408, Percent_Identity=26.2254901960784, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17560178, Length=461, Percent_Identity=25.5965292841649, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25147560, Length=376, Percent_Identity=25.2659574468085, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321563, Length=476, Percent_Identity=26.890756302521, Blast_Score=126, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6325403, Length=452, Percent_Identity=26.9911504424779, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI45553209, Length=437, Percent_Identity=29.0617848970252, Blast_Score=133, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 70806; Mature: 70806

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGLAGMLLASPRLHGEQLDALVRPMGAALRHRGPDDAGTWCDAQAGVALAHQRLSILDL
CCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHEEEC
SPLGHQPMRSADGRYVLAYNGEVYNFAQLRAALSALGHRFRGHSDTEVLLAAVVEWGLDE
CCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHH
TLTRCNGMFAIALWDERDNCLFLARDRVGKKPLYYGWAGDTLVFGSELKALWQHPDFDNG
HHHHHCCEEEEEEECCCCCEEEEECCCCCCCCEEEECCCCEEEECHHHHHHHCCCCCCCC
VDRDALTLLLRLGYIPAPACIHEHTFKLMPGRVLRIDAQAVAAGAAAHRPDQAQQPFWNA
CCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEEHHHHHHCHHHCCCCCCCCCHHHH
REAMQRALATPFTGTDAQAEEQLDRVLRDAVALRMVADVPVGVFLSGGTDSSIVTAMMQA
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHH
QSAQPVHTFSIGFEGSHHDEAPLAREVATHLCTDHTELYVSGADALAVVPNLPDMFDEPF
CCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCEEEECCCCCHHHCCCC
ADASQVPTALVARLARGGVTVALSGDGGDELFFGYGRYQRALRNWRMHGLVPGPLRRLMA
CCHHHHHHHHHHHHHCCCEEEEEECCCCCCEEECHHHHHHHHHCCHHCCCCCHHHHHHHH
VAARRNGESSRTGGLAALVAEAGARGIGDIYRNRISRWRDPAAVVLGATEPDSFYSLADP
HHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHCC
LHGSGTPADAMMLADFAAYLPDDLLCKVDRTTMAVGLEARAPLLDWRVAEFAWSLPLSLK
CCCCCCCHHHHHHHHHHHHCCCHHEEEECCHHEEECCCCCCCHHHHHHHHHHHCCCCEEE
YRDGVSKYLLKRVLCRYLPDAMVYRGKRGFGAPVSAWLRGDLHGWADDLLAHGALQREGV
ECCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHCCCCHHHHHHHHHCCHHHCCC
FAADSVARLWRDFNGGERKWHTHLWTVLMFQAWQAHWREQRAAVVR
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MCGLAGMLLASPRLHGEQLDALVRPMGAALRHRGPDDAGTWCDAQAGVALAHQRLSILDL
CCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHEEEC
SPLGHQPMRSADGRYVLAYNGEVYNFAQLRAALSALGHRFRGHSDTEVLLAAVVEWGLDE
CCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHH
TLTRCNGMFAIALWDERDNCLFLARDRVGKKPLYYGWAGDTLVFGSELKALWQHPDFDNG
HHHHHCCEEEEEEECCCCCEEEEECCCCCCCCEEEECCCCEEEECHHHHHHHCCCCCCCC
VDRDALTLLLRLGYIPAPACIHEHTFKLMPGRVLRIDAQAVAAGAAAHRPDQAQQPFWNA
CCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEEHHHHHHCHHHCCCCCCCCCHHHH
REAMQRALATPFTGTDAQAEEQLDRVLRDAVALRMVADVPVGVFLSGGTDSSIVTAMMQA
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHH
QSAQPVHTFSIGFEGSHHDEAPLAREVATHLCTDHTELYVSGADALAVVPNLPDMFDEPF
CCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCEEEECCCCCHHHCCCC
ADASQVPTALVARLARGGVTVALSGDGGDELFFGYGRYQRALRNWRMHGLVPGPLRRLMA
CCHHHHHHHHHHHHHCCCEEEEEECCCCCCEEECHHHHHHHHHCCHHCCCCCHHHHHHHH
VAARRNGESSRTGGLAALVAEAGARGIGDIYRNRISRWRDPAAVVLGATEPDSFYSLADP
HHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHCC
LHGSGTPADAMMLADFAAYLPDDLLCKVDRTTMAVGLEARAPLLDWRVAEFAWSLPLSLK
CCCCCCCHHHHHHHHHHHHCCCHHEEEECCHHEEECCCCCCCHHHHHHHHHHHCCCCEEE
YRDGVSKYLLKRVLCRYLPDAMVYRGKRGFGAPVSAWLRGDLHGWADDLLAHGALQREGV
ECCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHCCCCHHHHHHHHHCCHHHCCC
FAADSVARLWRDFNGGERKWHTHLWTVLMFQAWQAHWREQRAAVVR
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]