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The map label for this gene is trmD [H]

Identifier: 21242047

GI number: 21242047

Start: 1482696

End: 1483454

Strand: Direct

Name: trmD [H]

Synonym: XAC1294

Alternate gene names: 21242047

Gene position: 1482696-1483454 (Clockwise)

Preceding gene: 21242046

Following gene: 21242048

Centisome position: 28.65

GC content: 67.19

Gene sequence:

>759_bases
GTGCGCATCGACGTCATCAGCCTGTTCCCCGAGTTCATCGCGCAATGCGCGGCGTTCGGCGTGGTCGGGCGGGCGCAGGA
GCGCGGCTTGCTGGAATTGCAGGGCTGGAACCCGCGCGAGCATGCGCAGGGCAACTATCGCCGCGTGGACGACCGTCCGT
TCGGTGGCGGGCCCGGCATGGTGATGCTGATCGAGCCGCTGCGGGCCTGCCTGGATGCGGTGCAGGCCGCAGACGCGCGG
CCAGCGCCGGTGATCTATCTCAGCCCGCAGGGGCGGCCGCTCACGCAGGTGCTCGCACGCGAGTTGGCGCAGTTGCCGCG
CATGGTGTTGGTGTGTGGCCGCTATGAGGGCGTGGACGAGCGCTTTCTGGCGCAGGCGGTCGATATGGAAATCTCCATCG
GCGACTACGTATTGTCCGGCGGTGAATTGGGTGCAGCGGTTGTCGTGGACGTTGTGGCGCGGTTGCAGGACGGCGTATTG
AACGACGCCGAATCTGCCGCCCAGGACAGTTTTGAGGGCCCGCAAGGCCTGCTCGATTGCCCGCATTACAGCCATCCGGC
GACCCATGCATGGGGCGATGTGCCGGATGTGCTGCGATCAGGCAACCATGCGGCAATTGCGCGCTGGCGCCGGAAGCAGT
CGCTGGGCCGGACCTGGTTGCGCCGCCCGGACCTGCTCGACGAGGCGGGGCTGGAGAAGAACGATCGCCGTCTGCTGGAC
GAGTTTCGCCGCGAACTCGCCCCGGGCGACGAGAAATAA

Upstream 100 bases:

>100_bases
CAACCTGATCGTGGTGGACTGGGATCCGGATTTCTGATCAAAGCGGTGCCTCGGTGCTGCTCTTGCTCTCCCATTCCCTA
ACTCGACTCCCGATTCTCCA

Downstream 100 bases:

>100_bases
GGGCTCACCCCTAGCTCTTGCAAGATGGATTGGGGTACAATATGCGGCTTGCTGGCCAGCCTTGCGTCTGGCCCTCCTAC
GAACCTCGAGCAATCGCCGT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MRIDVISLFPEFIAQCAAFGVVGRAQERGLLELQGWNPREHAQGNYRRVDDRPFGGGPGMVMLIEPLRACLDAVQAADAR
PAPVIYLSPQGRPLTQVLARELAQLPRMVLVCGRYEGVDERFLAQAVDMEISIGDYVLSGGELGAAVVVDVVARLQDGVL
NDAESAAQDSFEGPQGLLDCPHYSHPATHAWGDVPDVLRSGNHAAIARWRRKQSLGRTWLRRPDLLDEAGLEKNDRRLLD
EFRRELAPGDEK

Sequences:

>Translated_252_residues
MRIDVISLFPEFIAQCAAFGVVGRAQERGLLELQGWNPREHAQGNYRRVDDRPFGGGPGMVMLIEPLRACLDAVQAADAR
PAPVIYLSPQGRPLTQVLARELAQLPRMVLVCGRYEGVDERFLAQAVDMEISIGDYVLSGGELGAAVVVDVVARLQDGVL
NDAESAAQDSFEGPQGLLDCPHYSHPATHAWGDVPDVLRSGNHAAIARWRRKQSLGRTWLRRPDLLDEAGLEKNDRRLLD
EFRRELAPGDEK
>Mature_252_residues
MRIDVISLFPEFIAQCAAFGVVGRAQERGLLELQGWNPREHAQGNYRRVDDRPFGGGPGMVMLIEPLRACLDAVQAADAR
PAPVIYLSPQGRPLTQVLARELAQLPRMVLVCGRYEGVDERFLAQAVDMEISIGDYVLSGGELGAAVVVDVVARLQDGVL
NDAESAAQDSFEGPQGLLDCPHYSHPATHAWGDVPDVLRSGNHAAIARWRRKQSLGRTWLRRPDLLDEAGLEKNDRRLLD
EFRRELAPGDEK

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=252, Percent_Identity=53.968253968254, Blast_Score=257, Evalue=4e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 27811; Mature: 27811

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDVISLFPEFIAQCAAFGVVGRAQERGLLELQGWNPREHAQGNYRRVDDRPFGGGPGM
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHCCCCCEECCCCCCCCCCCH
VMLIEPLRACLDAVQAADARPAPVIYLSPQGRPLTQVLARELAQLPRMVLVCGRYEGVDE
HHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHH
RFLAQAVDMEISIGDYVLSGGELGAAVVVDVVARLQDGVLNDAESAAQDSFEGPQGLLDC
HHHHHHHCCEEECCCEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCC
PHYSHPATHAWGDVPDVLRSGNHAAIARWRRKQSLGRTWLRRPDLLDEAGLEKNDRRLLD
CCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHH
EFRRELAPGDEK
HHHHHCCCCCCC
>Mature Secondary Structure
MRIDVISLFPEFIAQCAAFGVVGRAQERGLLELQGWNPREHAQGNYRRVDDRPFGGGPGM
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHCCCCCEECCCCCCCCCCCH
VMLIEPLRACLDAVQAADARPAPVIYLSPQGRPLTQVLARELAQLPRMVLVCGRYEGVDE
HHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHH
RFLAQAVDMEISIGDYVLSGGELGAAVVVDVVARLQDGVLNDAESAAQDSFEGPQGLLDC
HHHHHHHCCEEECCCEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCC
PHYSHPATHAWGDVPDVLRSGNHAAIARWRRKQSLGRTWLRRPDLLDEAGLEKNDRRLLD
CCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHH
EFRRELAPGDEK
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA