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The map label for this gene is mii [H]

Identifier: 21241896

GI number: 21241896

Start: 1292470

End: 1293669

Strand: Direct

Name: mii [H]

Synonym: XAC1142

Alternate gene names: 21241896

Gene position: 1292470-1293669 (Clockwise)

Preceding gene: 21241895

Following gene: 77748560

Centisome position: 24.97

GC content: 65.92

Gene sequence:

>1200_bases
ATGCCGCATCTTCCCCAACTCCGCATCCCCGCCACCTACATGCGCGGGGGCACCAGCAAAGGCGTGTTTTTCCGTCTGCA
GGATCTGCCGACCGCCGCGCAGCAGCCGGGGGCTGCACGCGATGCGTTGTTGTTGCGGGTGATCGGCAGCCCGGATCCGT
ATGCCAAGCAGATCGATGGCATGGGCGGGGCCACATCGAGCACCAGCAAGAGCGTGATCGTGTCGGCCAGCACGCGCGAT
GGGCATGATGTGGACTACCTGTTCGGGCAGGTGTCCATCGATAGCGCGTTCGTGGATTGGAGCGGTAACTGCGGCAATCT
GTCGGCGGCGGTGGGGCCATTCGCCATCGCCAGCGGCTTGATCGATCCCGCACGCGTGCCACGCGATGGTGTGGCCACGG
TGCGGATCTGGCAGGCCAATATCGGCAAGACCATCGTGGCGCATGTGCCGATGACCGGTGGGCTGGTGCAGGAGACCGGC
GACTTTGAACTGGATGGGGTGACCTTTCCGGCGGCGGAAGTGCAGCTGGAATTTCTCGACCCGGCCGACGATGCCGAAGG
CGACGGCGGGGCGATGTTTCCCACCGGCAACGTCATCGATCAACTGGAGATTCCCGGGCTGGGCACGCTCGATGCCACGC
TGATCAATGCGGGCATTCCCACCATCTTCGTCAACGCGCGCGACCTGGGTTACACCGGGACCGAGCTGCAGGACGCCATC
AATGGCGACCCGCGCGCCTTGACCATGTTCGAGACATTGCGCGCGCACGGTGCGGTGCGGATGGGTTTGATTGCGAAGGT
GGAAGACGCCGCGACGCGGCAACACACGCCCAAGGTCGCCTTCGTGGCACCGCCGGCCGACTACACCGCGTCCAGCCGCA
AGCCGGTGCACGCGGCAGAAATCGATCTGCTGGTGCGTGCGATGTCGATGGGCAAATTGCATCACGCGATGATGGGCACT
GCAGCAGTTGCGATCGGCACCGCTGCGGCCGTGCCGGGCACGCTGGTCAATCTGGCTGCCGGCGGTGCGGCGCGCTCGGC
GGTGCGATTCGGGCATCCGTCCGGCACCTTGCGGGTCGGCGCGCAGGCGCAGCTGGTCGAGGGCCAGTGGCAGGTGACCA
AGGCGCTGATGAGTCGCAGTGCGCGGGTGTTGATGGAGGGTTGGGTCAGGGTGCCGGCGCCTGTTGCCGAGGTTGGATAA

Upstream 100 bases:

>100_bases
ACAATGAACTGCATAACGCACGCGTGGTGCTGAGCGCTTGCACTGCTGCAATCGATATCCCCTTTGCTGCGACTCCATCC
AGTGAGCTAACCGAGGTTCC

Downstream 100 bases:

>100_bases
GCCTGTAAAGGGGGATATGGCCAGCGCCGCTAACGGAGCTTCCCACTTGCCGTCGTATAAATCGCAAGACTGCGTTGCAT
TAATTAGAACACGTTTTCGA

Product: hypothetical protein

Products: NA

Alternate protein names: (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MPHLPQLRIPATYMRGGTSKGVFFRLQDLPTAAQQPGAARDALLLRVIGSPDPYAKQIDGMGGATSSTSKSVIVSASTRD
GHDVDYLFGQVSIDSAFVDWSGNCGNLSAAVGPFAIASGLIDPARVPRDGVATVRIWQANIGKTIVAHVPMTGGLVQETG
DFELDGVTFPAAEVQLEFLDPADDAEGDGGAMFPTGNVIDQLEIPGLGTLDATLINAGIPTIFVNARDLGYTGTELQDAI
NGDPRALTMFETLRAHGAVRMGLIAKVEDAATRQHTPKVAFVAPPADYTASSRKPVHAAEIDLLVRAMSMGKLHHAMMGT
AAVAIGTAAAVPGTLVNLAAGGAARSAVRFGHPSGTLRVGAQAQLVEGQWQVTKALMSRSARVLMEGWVRVPAPVAEVG

Sequences:

>Translated_399_residues
MPHLPQLRIPATYMRGGTSKGVFFRLQDLPTAAQQPGAARDALLLRVIGSPDPYAKQIDGMGGATSSTSKSVIVSASTRD
GHDVDYLFGQVSIDSAFVDWSGNCGNLSAAVGPFAIASGLIDPARVPRDGVATVRIWQANIGKTIVAHVPMTGGLVQETG
DFELDGVTFPAAEVQLEFLDPADDAEGDGGAMFPTGNVIDQLEIPGLGTLDATLINAGIPTIFVNARDLGYTGTELQDAI
NGDPRALTMFETLRAHGAVRMGLIAKVEDAATRQHTPKVAFVAPPADYTASSRKPVHAAEIDLLVRAMSMGKLHHAMMGT
AAVAIGTAAAVPGTLVNLAAGGAARSAVRFGHPSGTLRVGAQAQLVEGQWQVTKALMSRSARVLMEGWVRVPAPVAEVG
>Mature_398_residues
PHLPQLRIPATYMRGGTSKGVFFRLQDLPTAAQQPGAARDALLLRVIGSPDPYAKQIDGMGGATSSTSKSVIVSASTRDG
HDVDYLFGQVSIDSAFVDWSGNCGNLSAAVGPFAIASGLIDPARVPRDGVATVRIWQANIGKTIVAHVPMTGGLVQETGD
FELDGVTFPAAEVQLEFLDPADDAEGDGGAMFPTGNVIDQLEIPGLGTLDATLINAGIPTIFVNARDLGYTGTELQDAIN
GDPRALTMFETLRAHGAVRMGLIAKVEDAATRQHTPKVAFVAPPADYTASSRKPVHAAEIDLLVRAMSMGKLHHAMMGTA
AVAIGTAAAVPGTLVNLAAGGAARSAVRFGHPSGTLRVGAQAQLVEGQWQVTKALMSRSARVLMEGWVRVPAPVAEVG

Specific function: Catalyzes the reversible isomerization of (R)-3- methylitaconate to 2,3-dimethylmaleate. Has very low isomerase activity with itaconate [H]

COG id: COG2828

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786985, Length=386, Percent_Identity=33.6787564766839, Blast_Score=163, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007400 [H]

Pfam domain/function: PF04303 PrpF [H]

EC number: =5.3.3.6 [H]

Molecular weight: Translated: 41516; Mature: 41385

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHLPQLRIPATYMRGGTSKGVFFRLQDLPTAAQQPGAARDALLLRVIGSPDPYAKQIDG
CCCCCCCCCCHHHHCCCCCCCEEEEEECCCCHHCCCCCCCCEEEEEEECCCCHHHHHHCC
MGGATSSTSKSVIVSASTRDGHDVDYLFGQVSIDSAFVDWSGNCGNLSAAVGPFAIASGL
CCCCCCCCCCEEEEEECCCCCCCEEEEEEEEEECEEEEECCCCCCCCCHHHHHHHHHHCC
IDPARVPRDGVATVRIWQANIGKTIVAHVPMTGGLVQETGDFELDGVTFPAAEVQLEFLD
CCHHHCCCCCCEEEEEEECCCCCEEEEECCCCCCEEECCCCEEECCEECCHHEEEEEEEC
PADDAEGDGGAMFPTGNVIDQLEIPGLGTLDATLINAGIPTIFVNARDLGYTGTELQDAI
CCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCHHHHHH
NGDPRALTMFETLRAHGAVRMGLIAKVEDAATRQHTPKVAFVAPPADYTASSRKPVHAAE
CCCCCEEHHHHHHHHCCCEEEHHEEEHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHH
IDLLVRAMSMGKLHHAMMGTAAVAIGTAAAVPGTLVNLAAGGAARSAVRFGHPSGTLRVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCHHHHEECCCCCCCEEEC
AQAQLVEGQWQVTKALMSRSARVLMEGWVRVPAPVAEVG
CEEEEECCHHHHHHHHHHHHHHHHHHCCEECCCCHHHCC
>Mature Secondary Structure 
PHLPQLRIPATYMRGGTSKGVFFRLQDLPTAAQQPGAARDALLLRVIGSPDPYAKQIDG
CCCCCCCCCHHHHCCCCCCCEEEEEECCCCHHCCCCCCCCEEEEEEECCCCHHHHHHCC
MGGATSSTSKSVIVSASTRDGHDVDYLFGQVSIDSAFVDWSGNCGNLSAAVGPFAIASGL
CCCCCCCCCCEEEEEECCCCCCCEEEEEEEEEECEEEEECCCCCCCCCHHHHHHHHHHCC
IDPARVPRDGVATVRIWQANIGKTIVAHVPMTGGLVQETGDFELDGVTFPAAEVQLEFLD
CCHHHCCCCCCEEEEEEECCCCCEEEEECCCCCCEEECCCCEEECCEECCHHEEEEEEEC
PADDAEGDGGAMFPTGNVIDQLEIPGLGTLDATLINAGIPTIFVNARDLGYTGTELQDAI
CCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCHHHHHH
NGDPRALTMFETLRAHGAVRMGLIAKVEDAATRQHTPKVAFVAPPADYTASSRKPVHAAE
CCCCCEEHHHHHHHHCCCEEEHHEEEHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHH
IDLLVRAMSMGKLHHAMMGTAAVAIGTAAAVPGTLVNLAAGGAARSAVRFGHPSGTLRVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCHHHHEECCCCCCCEEEC
AQAQLVEGQWQVTKALMSRSARVLMEGWVRVPAPVAEVG
CEEEEECCHHHHHHHHHHHHHHHHHHCCEECCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA