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The map label for this gene is insF1 [C]

Identifier: 21241814

GI number: 21241814

Start: 1210250

End: 1211065

Strand: Reverse

Name: insF1 [C]

Synonym: XAC1052

Alternate gene names: 21241814

Gene position: 1211065-1210250 (Counterclockwise)

Preceding gene: 21241815

Following gene: 21241812

Centisome position: 23.4

GC content: 62.75

Gene sequence:

>816_bases
GTGTGCGCGATGTGCCGGGTTTTGCGGGTGAACCGGGCTGGATACTACGCGTGGCTAAAGTCGCCCGACAGTGAACGCGC
CAAGGAAGACGAACGCCTGCTGGGGCTGATCAAGCACCACTGGTTGGCCAGCGGCAGTGTCTATGGGCATCGCAAGATTG
CCAAGGATCTACGCGATCTGGGTGAGCGTTGCAGTCGCCATCGGGTGCATCGATTGATGCGCACCGAGGGACTGCGTGCC
CAGGTGGGCTATGGCCGCAAGCCGCGCTTCCATGGCGGAACGCCGTGCAAGGCGGCAGCCAACCTGCTGGATCGACAGTT
CGACGTGACCGAGCCGGACACGGCCTGGGCGAGCGACTTCACCTTCATCCGTACGCATGAAGGCTGGATGTACCTGGCTG
TGGTGATCGATCTGTTTTCCAGGCAGGTCGTCGGCTGGGCGATGCGCGATCGAGCCGATACCGAGTTGGTCGTGCAGGCC
TTGCTGTCGGCGGTGTGGCGGCGCAAACCCAGCGCTGGTTGCCTGGTTCATTCGGACCAGGGGTCTGTCTACACCAGCGA
TGACTGGCGCAGTTTCCTGGCGTCCCATGGCTTGGTGTGCAGCATGAGTCGGCGTGGCAACTGCCACGAGCGCGAAGCCT
GCCCTTGGGGCACAACGCACCGGTGGAGAGCTTCTTTGGTCTGCTCAAACGCGAGCGGATCAGGCGGCGGATCTACCCCA
CCAAGGACGCCGCACGCGCCGAGGTGTTCGACTACATCGAGATGTTCTACAACCCACAACGCCGTCATGGTTCAACTGGC
GACCTGTCCCCTGTAG

Upstream 100 bases:

>100_bases
TGACGGAGGAGCGCGACATCCTAAAAAAGGCCGCCGCGTACTTTGCCAAGGGGTAAAGGCAAAGTACCTCTTCATGCAAG
TCCACCGCGAAGAATTCAGG

Downstream 100 bases:

>100_bases
AGTTTGAACGGCGCTACGCGCAACGAGGGTCTTGAGTGTCTACGGAACCCTGGGCGTATCAATGGAACGGCACTCACTCT
CCCCTCTGAGCAGCCCATCC

Product: ISxac3 transposase

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MCAMCRVLRVNRAGYYAWLKSPDSERAKEDERLLGLIKHHWLASGSVYGHRKIAKDLRDLGERCSRHRVHRLMRTEGLRA
QVGYGRKPRFHGGTPCKAAANLLDRQFDVTEPDTAWASDFTFIRTHEGWMYLAVVIDLFSRQVVGWAMRDRADTELVVQA
LLSAVWRRKPSAGCLVHSDQGSVYTSDDWRSFLASHGLVCSMSRRGNCHEREACPWGTTHRWRASLVCSNASGSGGGSTP
PRTPHAPRCSTTSRCSTTHNAVMVQLATCPL

Sequences:

>Translated_271_residues
MCAMCRVLRVNRAGYYAWLKSPDSERAKEDERLLGLIKHHWLASGSVYGHRKIAKDLRDLGERCSRHRVHRLMRTEGLRA
QVGYGRKPRFHGGTPCKAAANLLDRQFDVTEPDTAWASDFTFIRTHEGWMYLAVVIDLFSRQVVGWAMRDRADTELVVQA
LLSAVWRRKPSAGCLVHSDQGSVYTSDDWRSFLASHGLVCSMSRRGNCHEREACPWGTTHRWRASLVCSNASGSGGGSTP
PRTPHAPRCSTTSRCSTTHNAVMVQLATCPL
>Mature_271_residues
MCAMCRVLRVNRAGYYAWLKSPDSERAKEDERLLGLIKHHWLASGSVYGHRKIAKDLRDLGERCSRHRVHRLMRTEGLRA
QVGYGRKPRFHGGTPCKAAANLLDRQFDVTEPDTAWASDFTFIRTHEGWMYLAVVIDLFSRQVVGWAMRDRADTELVVQA
LLSAVWRRKPSAGCLVHSDQGSVYTSDDWRSFLASHGLVCSMSRRGNCHEREACPWGTTHRWRASLVCSNASGSGGGSTP
PRTPHAPRCSTTSRCSTTHNAVMVQLATCPL

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=213, Percent_Identity=33.3333333333333, Blast_Score=114, Evalue=7e-26,
Organism=Escherichia coli, GI1788405, Length=210, Percent_Identity=37.6190476190476, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787263, Length=210, Percent_Identity=37.6190476190476, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1786753, Length=210, Percent_Identity=37.6190476190476, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1786570, Length=210, Percent_Identity=37.6190476190476, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1786490, Length=210, Percent_Identity=37.6190476190476, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1789981, Length=220, Percent_Identity=30, Blast_Score=101, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 30395; Mature: 30395

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
4.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCAMCRVLRVNRAGYYAWLKSPDSERAKEDERLLGLIKHHWLASGSVYGHRKIAKDLRDL
CCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
GERCSRHRVHRLMRTEGLRAQVGYGRKPRFHGGTPCKAAANLLDRQFDVTEPDTAWASDF
HHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHCCC
TFIRTHEGWMYLAVVIDLFSRQVVGWAMRDRADTELVVQALLSAVWRRKPSAGCLVHSDQ
EEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEECCC
GSVYTSDDWRSFLASHGLVCSMSRRGNCHEREACPWGTTHRWRASLVCSNASGSGGGSTP
CCEEECHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCC
PRTPHAPRCSTTSRCSTTHNAVMVQLATCPL
CCCCCCCCCCCCCCCCCCCCEEEEEEECCCC
>Mature Secondary Structure
MCAMCRVLRVNRAGYYAWLKSPDSERAKEDERLLGLIKHHWLASGSVYGHRKIAKDLRDL
CCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
GERCSRHRVHRLMRTEGLRAQVGYGRKPRFHGGTPCKAAANLLDRQFDVTEPDTAWASDF
HHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHCCC
TFIRTHEGWMYLAVVIDLFSRQVVGWAMRDRADTELVVQALLSAVWRRKPSAGCLVHSDQ
EEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEECCC
GSVYTSDDWRSFLASHGLVCSMSRRGNCHEREACPWGTTHRWRASLVCSNASGSGGGSTP
CCEEECHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCC
PRTPHAPRCSTTSRCSTTHNAVMVQLATCPL
CCCCCCCCCCCCCCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]