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The map label for this gene is rpoA [H]

Identifier: 21241761

GI number: 21241761

Start: 1147785

End: 1148783

Strand: Direct

Name: rpoA [H]

Synonym: XAC0996

Alternate gene names: 21241761

Gene position: 1147785-1148783 (Clockwise)

Preceding gene: 21241760

Following gene: 21241762

Centisome position: 22.18

GC content: 62.06

Gene sequence:

>999_bases
ATGACGGTTACCGCCAACCAGGTTCTGCGCCCCCGTGGTCCGCAGATCGAACGTCTTACCGACAACCGTGCCAAGGTCGT
GATCGAGCCCTTGGAGCGCGGTTACGGGCACACGTTGGGCAATGCCCTGCGTCGTGTGTTGTTGTCGTCGATCCCCGGTT
TTGCGATCACCGAGGTCGAGATCGACGGTGTGCTCCACGAGTACACCACGGTCGAAGGTCTGCAGGAAGACGTGCTCGAC
GTCCTGCTGAACCTGAAAGACGTGGCGATTCGTATGCACAGTGGCGATAGCGCCACGCTGTCGTTGTCCAAGCAGGGTCC
GGGTACGGTCACTGCGGCAGACATCAGGACCGATCACAACGTCGAGATCATCAACGGCGACCACGTGATCTGCCATCTGA
CCAAGGACACGGCGCTGAACATGCGCCTCAAGATCGAGCGCGGTTTCGGCTATCAGCCGGCTGCTGCGCGTCGTCGTCCG
GACGAAGAGACCCGCACCATCGGTCGTCTGATGCTGGATGCGTCGTTCTCGCCCGTGCGTCGCGTTGCCTACGCCGTGGA
AGCTGCGCGTGTTGAACAGCGCACCGACCTCGACAAGCTGGTGATCGATATCGAAACCAACGGCACGATCGATGCAGAGG
AAGCCGTGCGCACCGCCGCCGACATCCTTAGCGATCAGCTGTCGGTGTTCGGCGATTTCACCCACCGCGATCGCGGTGCG
GCCAAGCCGGCTGCCAGCGGCGTGGATCCGGTGCTGCTGCGTCCGATCGACGACCTCGAGCTGACCGTGCGTTCGGCCAA
CTGCCTGAAGGCCGAGAGCATCTACTACATCGGCGATTTGATCCAGAAGACCGAAGTCGAGCTGCTCAAGACCCCGAATC
TGGGCAAGAAGTCGCTGACCGAAATCAAGGAAGTGCTGGCGCAGCGCGGCCTCGCTCTGGGCATGAAGCTGGAGAACTGG
CCGCCGGCCGGTGTCGCCCAGCACGGCATGCTTGGTTAA

Upstream 100 bases:

>100_bases
CTTCGGATATCAACGAAGCGCTGATCGTCGAGTTGTATTCGAAGTAATTCACATTGGAGAACCTCCGGTACTGCCGGAGG
TTCGCAGGAGACCCCGCAAC

Downstream 100 bases:

>100_bases
TGCGTCACCGCATGGGCGTCTTCGGGCGCCCATGCTGTTTTTCTACATCGACGGGCCGAAGCCCGCGATGACATCCGGTC
AAAGGACGGCCGGCTCGGAC

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MTVTANQVLRPRGPQIERLTDNRAKVVIEPLERGYGHTLGNALRRVLLSSIPGFAITEVEIDGVLHEYTTVEGLQEDVLD
VLLNLKDVAIRMHSGDSATLSLSKQGPGTVTAADIRTDHNVEIINGDHVICHLTKDTALNMRLKIERGFGYQPAAARRRP
DEETRTIGRLMLDASFSPVRRVAYAVEAARVEQRTDLDKLVIDIETNGTIDAEEAVRTAADILSDQLSVFGDFTHRDRGA
AKPAASGVDPVLLRPIDDLELTVRSANCLKAESIYYIGDLIQKTEVELLKTPNLGKKSLTEIKEVLAQRGLALGMKLENW
PPAGVAQHGMLG

Sequences:

>Translated_332_residues
MTVTANQVLRPRGPQIERLTDNRAKVVIEPLERGYGHTLGNALRRVLLSSIPGFAITEVEIDGVLHEYTTVEGLQEDVLD
VLLNLKDVAIRMHSGDSATLSLSKQGPGTVTAADIRTDHNVEIINGDHVICHLTKDTALNMRLKIERGFGYQPAAARRRP
DEETRTIGRLMLDASFSPVRRVAYAVEAARVEQRTDLDKLVIDIETNGTIDAEEAVRTAADILSDQLSVFGDFTHRDRGA
AKPAASGVDPVLLRPIDDLELTVRSANCLKAESIYYIGDLIQKTEVELLKTPNLGKKSLTEIKEVLAQRGLALGMKLENW
PPAGVAQHGMLG
>Mature_331_residues
TVTANQVLRPRGPQIERLTDNRAKVVIEPLERGYGHTLGNALRRVLLSSIPGFAITEVEIDGVLHEYTTVEGLQEDVLDV
LLNLKDVAIRMHSGDSATLSLSKQGPGTVTAADIRTDHNVEIINGDHVICHLTKDTALNMRLKIERGFGYQPAAARRRPD
EETRTIGRLMLDASFSPVRRVAYAVEAARVEQRTDLDKLVIDIETNGTIDAEEAVRTAADILSDQLSVFGDFTHRDRGAA
KPAASGVDPVLLRPIDDLELTVRSANCLKAESIYYIGDLIQKTEVELLKTPNLGKKSLTEIKEVLAQRGLALGMKLENWP
PAGVAQHGMLG

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family [H]

Homologues:

Organism=Escherichia coli, GI1789690, Length=327, Percent_Identity=63.914373088685, Blast_Score=427, Evalue=1e-121,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- ProDom:   PD001179 [H]

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 36364; Mature: 36233

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTANQVLRPRGPQIERLTDNRAKVVIEPLERGYGHTLGNALRRVLLSSIPGFAITEVE
CCCCCCCHHCCCCCCHHHHCCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEE
IDGVLHEYTTVEGLQEDVLDVLLNLKDVAIRMHSGDSATLSLSKQGPGTVTAADIRTDHN
ECHHHHHHHHHHHHHHHHHHHHHCHHHHEEEEECCCCEEEEEECCCCCCEEEEEECCCCC
VEIINGDHVICHLTKDTALNMRLKIERGFGYQPAAARRRPDEETRTIGRLMLDASFSPVR
EEEEECCEEEEEEECCCEEEEEEEEECCCCCCCHHHHCCCCHHHHHHHHHHHCCCCCHHH
RVAYAVEAARVEQRTDLDKLVIDIETNGTIDAEEAVRTAADILSDQLSVFGDFTHRDRGA
HHHHHHHHHHHHHHCCHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCHHHCCCCC
AKPAASGVDPVLLRPIDDLELTVRSANCLKAESIYYIGDLIQKTEVELLKTPNLGKKSLT
CCCCCCCCCCEEECCCCCCEEEEECCCCEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHH
EIKEVLAQRGLALGMKLENWPPAGVAQHGMLG
HHHHHHHHCCCEECEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
TVTANQVLRPRGPQIERLTDNRAKVVIEPLERGYGHTLGNALRRVLLSSIPGFAITEVE
CCCCCCHHCCCCCCHHHHCCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEE
IDGVLHEYTTVEGLQEDVLDVLLNLKDVAIRMHSGDSATLSLSKQGPGTVTAADIRTDHN
ECHHHHHHHHHHHHHHHHHHHHHCHHHHEEEEECCCCEEEEEECCCCCCEEEEEECCCCC
VEIINGDHVICHLTKDTALNMRLKIERGFGYQPAAARRRPDEETRTIGRLMLDASFSPVR
EEEEECCEEEEEEECCCEEEEEEEEECCCCCCCHHHHCCCCHHHHHHHHHHHCCCCCHHH
RVAYAVEAARVEQRTDLDKLVIDIETNGTIDAEEAVRTAADILSDQLSVFGDFTHRDRGA
HHHHHHHHHHHHHHCCHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCHHHCCCCC
AKPAASGVDPVLLRPIDDLELTVRSANCLKAESIYYIGDLIQKTEVELLKTPNLGKKSLT
CCCCCCCCCCEEECCCCCCEEEEECCCCEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHH
EIKEVLAQRGLALGMKLENWPPAGVAQHGMLG
HHHHHHHHCCCEECEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA