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The map label for this gene is colS [H]

Identifier: 21241605

GI number: 21241605

Start: 996163

End: 997434

Strand: Direct

Name: colS [H]

Synonym: XAC0835

Alternate gene names: 21241605

Gene position: 996163-997434 (Clockwise)

Preceding gene: 21241604

Following gene: 21241607

Centisome position: 19.25

GC content: 66.04

Gene sequence:

>1272_bases
ATGCCACACGGGCTCCCGAGAAAAATACGTCTGGCCTTCCTGCTGCAGGTCGCGCTGGCGAGCTTGGCGCTCGTGCTGGC
CGGGTATCTGGTGTCGTTCGTCATCAAATACAGCCTGGTGCGCACCGTGCTCGCCGACGAGGCCGTGCATTTCTGGCAGA
TGCAAAAGAGCGTGCCGGACAACCGGCCGCCGAATACGCGCAACATCCAGGGCTATTTTTCGCCCGCCGGTACCGCCGCC
GAGGCGGCTCCGGCCGCGCTGCGCCACCTGTCTCCGGGTTTCAGCGAAGTGGCTGCCGCCGATGCGCTGGTGTATGTCGA
TCAACGTCCGGAGGGGCGGCTGTATCTGGTGTTTCCGCGCTCGCGCGCGGCGCATCTGACCTGGTGGTTCGGTGTGGTGC
CGGCGATTGTGGTGTTGGTGGCAATCTACGGCGTGTCATGGTTCACCTACCGGGTTTCCAAGCGGCTGGTCTCGCCGATC
AGCTGGCTGGCCCGGCGCGTGTCGCATTGGGACCCGCGCAACCCGGATGTGGACGAACTGGCTCCGGAGCGGCTGCCTGC
CGAACTGCAGGGCGAAACGCGCCAGCTGGCCGCCGCGCTGCATGCGCTGGGGTTGCGCGTCAGCGAGCATGTCGCGCGCG
AACGCAACTTCACCCGCGATGCCAGCCATGAATTGCGCACTCCGCTCACGGTGATCCGCGTGGCCAGCGACATGGCCTTG
GCCGACGACGAATTGTCCGCGCGCACGCAGCGCAGCCTGCGCCGCATCCAGCGCGCCGGCCACGATATGGAGGCGGTGAT
CGATGCGTTCCTGATCCTGGCACGCGAAGCCGAGATCGACCCGCAGAGCGAAAACTTCGATGTGGCCGAGCTTGCCAGCG
AAGAACTGCAAAGCGCGCGTGAGCTGCTTGGCGACAAACCGGTGTCGTTGCGCATGGTCGGCGACCGCAGCCTGCAGATG
TTCGCCCCGCCCAGGGTGATGCGGGTGGTGCTCAGCAATCTGTTGCGTAACGCCTGCGCTTACACCGATACCGGCAGCAT
CGAGGTCGAGGTGACCCACGACCGTATCGTGGTCCGCGATACCGGCATTGGCATGAGCGAAGAAGCGCGGGCACGCGCGT
TCGAACCGTTCTTCCGCGCCGACCCTACCCGCCCGCAAGGCACTGGGCTTGGCCTGTCGATCGTCCGTCGGCTGTGCGAT
CGCTTCGGCTGGCGTATCGAACTGCAGAGCGAAGCCGGCGTCGGTACGTCCGTGGCGGTGGTCGTCGCCTGA

Upstream 100 bases:

>100_bases
GGCGCTCACGCGCCGATCGATCTCCTGTGCTGCCGGCTGGTTGCACGAAGGACTCACGGAAGTGCTGCCTATACTCGGGC
GCTCCTTCCTGGATGCGGCA

Downstream 100 bases:

>100_bases
GGCGGCATTCAGTGGTGCTGGCGCGTGCTGATGATGTCTTGCTGCCGGCCAAGTCGTCAGGCAGCGATGGCAGGCAACGA
CGAAGGCCTGCTGCACGCGG

Product: two-component system sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MPHGLPRKIRLAFLLQVALASLALVLAGYLVSFVIKYSLVRTVLADEAVHFWQMQKSVPDNRPPNTRNIQGYFSPAGTAA
EAAPAALRHLSPGFSEVAAADALVYVDQRPEGRLYLVFPRSRAAHLTWWFGVVPAIVVLVAIYGVSWFTYRVSKRLVSPI
SWLARRVSHWDPRNPDVDELAPERLPAELQGETRQLAAALHALGLRVSEHVARERNFTRDASHELRTPLTVIRVASDMAL
ADDELSARTQRSLRRIQRAGHDMEAVIDAFLILAREAEIDPQSENFDVAELASEELQSARELLGDKPVSLRMVGDRSLQM
FAPPRVMRVVLSNLLRNACAYTDTGSIEVEVTHDRIVVRDTGIGMSEEARARAFEPFFRADPTRPQGTGLGLSIVRRLCD
RFGWRIELQSEAGVGTSVAVVVA

Sequences:

>Translated_423_residues
MPHGLPRKIRLAFLLQVALASLALVLAGYLVSFVIKYSLVRTVLADEAVHFWQMQKSVPDNRPPNTRNIQGYFSPAGTAA
EAAPAALRHLSPGFSEVAAADALVYVDQRPEGRLYLVFPRSRAAHLTWWFGVVPAIVVLVAIYGVSWFTYRVSKRLVSPI
SWLARRVSHWDPRNPDVDELAPERLPAELQGETRQLAAALHALGLRVSEHVARERNFTRDASHELRTPLTVIRVASDMAL
ADDELSARTQRSLRRIQRAGHDMEAVIDAFLILAREAEIDPQSENFDVAELASEELQSARELLGDKPVSLRMVGDRSLQM
FAPPRVMRVVLSNLLRNACAYTDTGSIEVEVTHDRIVVRDTGIGMSEEARARAFEPFFRADPTRPQGTGLGLSIVRRLCD
RFGWRIELQSEAGVGTSVAVVVA
>Mature_422_residues
PHGLPRKIRLAFLLQVALASLALVLAGYLVSFVIKYSLVRTVLADEAVHFWQMQKSVPDNRPPNTRNIQGYFSPAGTAAE
AAPAALRHLSPGFSEVAAADALVYVDQRPEGRLYLVFPRSRAAHLTWWFGVVPAIVVLVAIYGVSWFTYRVSKRLVSPIS
WLARRVSHWDPRNPDVDELAPERLPAELQGETRQLAAALHALGLRVSEHVARERNFTRDASHELRTPLTVIRVASDMALA
DDELSARTQRSLRRIQRAGHDMEAVIDAFLILAREAEIDPQSENFDVAELASEELQSARELLGDKPVSLRMVGDRSLQMF
APPRVMRVVLSNLLRNACAYTDTGSIEVEVTHDRIVVRDTGIGMSEEARARAFEPFFRADPTRPQGTGLGLSIVRRLCDR
FGWRIELQSEAGVGTSVAVVVA

Specific function: Member of a two-component regulatory system qseB/qseC. Activates the flagella regulon by activating transcription of flhDC. May activate qseB by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=323, Percent_Identity=26.3157894736842, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1789403, Length=298, Percent_Identity=30.2013422818792, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI48994928, Length=232, Percent_Identity=29.7413793103448, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1786600, Length=219, Percent_Identity=28.310502283105, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI145693157, Length=217, Percent_Identity=31.3364055299539, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789149, Length=234, Percent_Identity=27.3504273504274, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787894, Length=246, Percent_Identity=30.4878048780488, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790346, Length=222, Percent_Identity=29.7297297297297, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI87081816, Length=214, Percent_Identity=29.4392523364486, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1789808, Length=287, Percent_Identity=26.4808362369338, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87082128, Length=297, Percent_Identity=26.9360269360269, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788713, Length=253, Percent_Identity=24.1106719367589, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1790861, Length=271, Percent_Identity=27.6752767527675, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1788393, Length=241, Percent_Identity=25.3112033195021, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 46992; Mature: 46861

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHGLPRKIRLAFLLQVALASLALVLAGYLVSFVIKYSLVRTVLADEAVHFWQMQKSVPD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NRPPNTRNIQGYFSPAGTAAEAAPAALRHLSPGFSEVAAADALVYVDQRPEGRLYLVFPR
CCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCHHHHHHHCEEEEEECCCCCCEEEEECC
SRAAHLTWWFGVVPAIVVLVAIYGVSWFTYRVSKRLVSPISWLARRVSHWDPRNPDVDEL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
APERLPAELQGETRQLAAALHALGLRVSEHVARERNFTRDASHELRTPLTVIRVASDMAL
CHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHH
ADDELSARTQRSLRRIQRAGHDMEAVIDAFLILAREAEIDPQSENFDVAELASEELQSAR
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
ELLGDKPVSLRMVGDRSLQMFAPPRVMRVVLSNLLRNACAYTDTGSIEVEVTHDRIVVRD
HHHCCCCEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEEE
TGIGMSEEARARAFEPFFRADPTRPQGTGLGLSIVRRLCDRFGWRIELQSEAGVGTSVAV
CCCCCCHHHHHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCEEE
VVA
EEC
>Mature Secondary Structure 
PHGLPRKIRLAFLLQVALASLALVLAGYLVSFVIKYSLVRTVLADEAVHFWQMQKSVPD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NRPPNTRNIQGYFSPAGTAAEAAPAALRHLSPGFSEVAAADALVYVDQRPEGRLYLVFPR
CCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCHHHHHHHCEEEEEECCCCCCEEEEECC
SRAAHLTWWFGVVPAIVVLVAIYGVSWFTYRVSKRLVSPISWLARRVSHWDPRNPDVDEL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
APERLPAELQGETRQLAAALHALGLRVSEHVARERNFTRDASHELRTPLTVIRVASDMAL
CHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHH
ADDELSARTQRSLRRIQRAGHDMEAVIDAFLILAREAEIDPQSENFDVAELASEELQSAR
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
ELLGDKPVSLRMVGDRSLQMFAPPRVMRVVLSNLLRNACAYTDTGSIEVEVTHDRIVVRD
HHHCCCCEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEEE
TGIGMSEEARARAFEPFFRADPTRPQGTGLGLSIVRRLCDRFGWRIELQSEAGVGTSVAV
CCCCCCHHHHHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCEEE
VVA
EEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796; 11929534 [H]