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The map label for this gene is dusB

Identifier: 21241586

GI number: 21241586

Start: 973973

End: 974971

Strand: Reverse

Name: dusB

Synonym: XAC0816

Alternate gene names: 21241586

Gene position: 974971-973973 (Counterclockwise)

Preceding gene: 21241587

Following gene: 21241585

Centisome position: 18.84

GC content: 67.47

Gene sequence:

>999_bases
ATGCAGATCGGCCCCTACTCCATCGCTCCCAAGGTGATCCTCGCGCCCATGGCGGGCGTCACCGACAAGCCGTTCCGGCT
GCTGTGCAAGCGGCTGGGCGCGGGCCTGGCGGTGTCGGAAATGACCATTTCCGACCCGCGCTTCTGGGGCACGCGCAAAT
CGCTGCACCGCATGGACCATGCCGGCGAGCCGGACCCGATCAGCGTGCAGATCGCCGGCACCGAGCCGCAGCAACTGGCC
GAGGCGGCGCGCTACAACGTCGACCACGGTGCGCAGTTGATCGACATCAACATGGGCTGCCCGGCCAAGAAAGTGTGCAA
TGCCTGGGCCGGCTCGGCACTGATGCGCGATGAAGAGCTGGTGGCACGCATCCTGACCGCGGTGGTGCAGGCAGTGAACG
TGCCGGTGACGTTGAAGATCCGCACCGGTTGGGATTGCGACCACCGCAACGGCCCGACGATCGCGCGGATCGCGCAGGAC
TGCGGTATCGCCGCGCTCGCCGTACACGGGCGCACACGCGACCAGCACTACACCGGCAGCGCCGAGTACGCGACGATTGC
GCAGATCAAGGCCGCGCTGCAGATCCCGGTGGTTGCCAATGGCGATATCGACTCGCCGCAGAAAGCCGCCCAGGTGCTGC
GCGAAACCGGCGCCGATGCCGTGATGATCGGCCGCGCCGCGCAAGGCCGCCCGTGGATCTTCGGCGAGGTGGCGCATTTT
TTGGCCACCGGCGAGGTATTGCCGCCGCCGTCGCTGGCGTTCGTGCGCGACACCTTGCTCGGCCACCTGGAGGCGCTGCA
CGCGTTTTATGGCCAACCGCAAGGCGTGCGCATCGCACGCAAGCATCTGGGTTGGTACGCCAAGGACCACCCGCAAAGCG
CCGACTTCCGGGCGGTGGTCAATCGCGCCGAAACCCCCGACGCGCAGTTGGCCCTGACGCGCGATTACTTCGATGCCTTG
ATCGCCGGCGTGCCGCCCGCACTATCGGACGCTGCCTGA

Upstream 100 bases:

>100_bases
GGCCGTTTCGCAGTAAGCGATACCACAGCGGCGGTGCCGGCCCGGCCGCCGCAGGGTTGGCAGCACCAAGTGCTGTCATC
GCTGAAGTTACACTAGCGGC

Downstream 100 bases:

>100_bases
GGTTTTCTGTCCTGCTGGAGCTGCATGCATGAGCGCACCGAGCGACACCCCGACCTATCTGCACGGCTTTTCCGCCACCG
AACAGGCGCGTCTGCTCAAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MQIGPYSIAPKVILAPMAGVTDKPFRLLCKRLGAGLAVSEMTISDPRFWGTRKSLHRMDHAGEPDPISVQIAGTEPQQLA
EAARYNVDHGAQLIDINMGCPAKKVCNAWAGSALMRDEELVARILTAVVQAVNVPVTLKIRTGWDCDHRNGPTIARIAQD
CGIAALAVHGRTRDQHYTGSAEYATIAQIKAALQIPVVANGDIDSPQKAAQVLRETGADAVMIGRAAQGRPWIFGEVAHF
LATGEVLPPPSLAFVRDTLLGHLEALHAFYGQPQGVRIARKHLGWYAKDHPQSADFRAVVNRAETPDAQLALTRDYFDAL
IAGVPPALSDAA

Sequences:

>Translated_332_residues
MQIGPYSIAPKVILAPMAGVTDKPFRLLCKRLGAGLAVSEMTISDPRFWGTRKSLHRMDHAGEPDPISVQIAGTEPQQLA
EAARYNVDHGAQLIDINMGCPAKKVCNAWAGSALMRDEELVARILTAVVQAVNVPVTLKIRTGWDCDHRNGPTIARIAQD
CGIAALAVHGRTRDQHYTGSAEYATIAQIKAALQIPVVANGDIDSPQKAAQVLRETGADAVMIGRAAQGRPWIFGEVAHF
LATGEVLPPPSLAFVRDTLLGHLEALHAFYGQPQGVRIARKHLGWYAKDHPQSADFRAVVNRAETPDAQLALTRDYFDAL
IAGVPPALSDAA
>Mature_332_residues
MQIGPYSIAPKVILAPMAGVTDKPFRLLCKRLGAGLAVSEMTISDPRFWGTRKSLHRMDHAGEPDPISVQIAGTEPQQLA
EAARYNVDHGAQLIDINMGCPAKKVCNAWAGSALMRDEELVARILTAVVQAVNVPVTLKIRTGWDCDHRNGPTIARIAQD
CGIAALAVHGRTRDQHYTGSAEYATIAQIKAALQIPVVANGDIDSPQKAAQVLRETGADAVMIGRAAQGRPWIFGEVAHF
LATGEVLPPPSLAFVRDTLLGHLEALHAFYGQPQGVRIARKHLGWYAKDHPQSADFRAVVNRAETPDAQLALTRDYFDAL
IAGVPPALSDAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily

Homologues:

Organism=Homo sapiens, GI239788483, Length=238, Percent_Identity=32.3529411764706, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI239788462, Length=238, Percent_Identity=32.3529411764706, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI40807366, Length=229, Percent_Identity=30.1310043668122, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI31742496, Length=227, Percent_Identity=31.2775330396476, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI8923374, Length=242, Percent_Identity=31.404958677686, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1789660, Length=318, Percent_Identity=55.6603773584906, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1788462, Length=233, Percent_Identity=36.9098712446352, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI145693211, Length=318, Percent_Identity=26.7295597484277, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17510279, Length=244, Percent_Identity=32.7868852459016, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17543114, Length=238, Percent_Identity=28.5714285714286, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17507177, Length=233, Percent_Identity=28.3261802575107, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25144369, Length=179, Percent_Identity=31.2849162011173, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323560, Length=236, Percent_Identity=31.3559322033898, Blast_Score=105, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6323433, Length=261, Percent_Identity=29.8850574712644, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323437, Length=233, Percent_Identity=26.1802575107296, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24585320, Length=236, Percent_Identity=34.3220338983051, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24580595, Length=273, Percent_Identity=32.6007326007326, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19920448, Length=273, Percent_Identity=32.6007326007326, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19921524, Length=229, Percent_Identity=27.5109170305677, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI45549423, Length=243, Percent_Identity=30.4526748971193, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUSB_XANAC (Q8PP72)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_641168.1
- ProteinModelPortal:   Q8PP72
- SMR:   Q8PP72
- GeneID:   1154887
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC0816
- NMPDR:   fig|190486.1.peg.812
- HOGENOM:   HBG557545
- OMA:   QLAGCEP
- ProtClustDB:   CLSK451089
- BioCyc:   XAXO190486:XAC0816-MONOMER
- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR11082
- PIRSF:   PIRSF006621
- TIGRFAMs:   TIGR00737

Pfam domain/function: PF01207 Dus

EC number: NA

Molecular weight: Translated: 35703; Mature: 35703

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIGPYSIAPKVILAPMAGVTDKPFRLLCKRLGAGLAVSEMTISDPRFWGTRKSLHRMDH
CCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHC
AGEPDPISVQIAGTEPQQLAEAARYNVDHGAQLIDINMGCPAKKVCNAWAGSALMRDEEL
CCCCCCEEEEEECCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHCHHHHCHHHH
VARILTAVVQAVNVPVTLKIRTGWDCDHRNGPTIARIAQDCGIAALAVHGRTRDQHYTGS
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHCCEEEEEEECCCCCCCCCCC
AEYATIAQIKAALQIPVVANGDIDSPQKAAQVLRETGADAVMIGRAAQGRPWIFGEVAHF
CCHHHHHHHHHHHEECEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHH
LATGEVLPPPSLAFVRDTLLGHLEALHAFYGQPQGVRIARKHLGWYAKDHPQSADFRAVV
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCHHHHHH
NRAETPDAQLALTRDYFDALIAGVPPALSDAA
HCCCCCCCCEEEHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MQIGPYSIAPKVILAPMAGVTDKPFRLLCKRLGAGLAVSEMTISDPRFWGTRKSLHRMDH
CCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHC
AGEPDPISVQIAGTEPQQLAEAARYNVDHGAQLIDINMGCPAKKVCNAWAGSALMRDEEL
CCCCCCEEEEEECCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHCHHHHCHHHH
VARILTAVVQAVNVPVTLKIRTGWDCDHRNGPTIARIAQDCGIAALAVHGRTRDQHYTGS
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHCCEEEEEEECCCCCCCCCCC
AEYATIAQIKAALQIPVVANGDIDSPQKAAQVLRETGADAVMIGRAAQGRPWIFGEVAHF
CCHHHHHHHHHHHEECEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHH
LATGEVLPPPSLAFVRDTLLGHLEALHAFYGQPQGVRIARKHLGWYAKDHPQSADFRAVV
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCHHHHHH
NRAETPDAQLALTRDYFDALIAGVPPALSDAA
HCCCCCCCCEEEHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217