Click here to switch to the map view.

The map label for this gene is purH

Identifier: 21241284

GI number: 21241284

Start: 603120

End: 604703

Strand: Reverse

Name: purH

Synonym: XAC0513

Alternate gene names: 21241284

Gene position: 604703-603120 (Counterclockwise)

Preceding gene: 21241293

Following gene: 21241283

Centisome position: 11.68

GC content: 66.67

Gene sequence:

>1584_bases
ATGGCCTCTGATTTCCTGCCCGTGCGCCGGGCCCTGCTCTCCGTTTCCGACAAGACCGGCCTGATCGACCTGGCCCGCGC
GCTGGTGGCGCGCAACGTCGAGCTGCTGTCCACCGGCGGCACCGCCAAGGCGATCCGCGAAGCCGGCCTGCCGGTGAAGG
ACGTCGCCGAGCTGACCGGTTTTCCGGAAATGATGGATGGCCGCGTCAAGACGCTGCACCCGCTGGTGCACGGCGGCCTG
CTCGGACGCGCCGGTGTCGACGAAGCGGTGATGGCCGAGCACGGCATCGTGCCGATCGACCTGCTGGTGCTGAACCTGTA
TCCGTTCGAGTCGGTCACCGTGAAAGCCGATTGCACGCTGGCCGATGCGGTGGAAAACATCGACATCGGCGGCCCGGCGA
TGTTGCGCAGCGCGGCCAAGAATTTCGCGCGCGTGGCCGTGGCCGCCGACCCGGCGCAGTACGCCGACCTGCTGGTCGAA
CTGGAGGCCAACGACGGCCAGCTGTCGGCGGCCAAGCGCTTTGCGCTGTCGGTGGCTGCGTTCAATCGCGTGGCGCAGTA
TGACGCGGCGATCAGCAACTATCTCTCGGCAGTGGCCGACAGCGCCGAGGCAGTGCCCACGCGCAGCCCGTTCCCGGCGC
AGATCAATTCCAACTTCGTCAAGGTGATGGACCTGCGTTACGGCGAGAACCCGCACCAGTCCGGCGCGTTCTACCGCGAC
CTGTATCCGGTGCCGGGCACGCTGGCCACCTTCCAGCAGTTGCAGGGCAAGGAACTGAGCTACAACAACCTGGCCGATGC
CGATGCGGCGTGGGAATGCGTGCGCCAGTTCGACGCGCCGGCCTGCGTGATCGTCAAGCACGCCAACCCCTGCGGCGTGG
CGGTGGGCGCGGGCTGCGGCGATGCCTATGAGCTGGCCTACGCCACCGACCCCACCAGCGCGTTCGGCGGCATCCTGGCG
TTCAACAAGACCCTTGACGCAGCGACGGCAAAAGCCATCCTCGATCGCCAGTTCGTCGAAGTGCTGATCGCGCCCGACTA
CGAGGCCGGCGCGCTGGAATACGCCACCAAGAAGGCCAACGTGCGCGTGCTCAAGATTCCGCACGGCAATGGCCTCAACA
ACTACGACACCAAGCGGATCGGCTCGGGCCTGTTGATGCAATCGGCCGACAACCGCGGCATGTCGCAGGGCGAGTTGAGC
GTGGTCACCCAGCGTGCGCCCAACGAGGCGGAGCTGGGCGACCTGCTGTTTGCCTGGCGCGTGGCCAAGTACGTCAAATC
CAACGCGATCGTCTATGCCAAGGACAACCGCACCATTGGCGTGGGCGCCGGGCAGATGAGCCGCGTGGTCAGCGCCAAGA
TCGCTGCCCTCAAGGCCGAAGAAGCCAAGCTCACCGTGGCCGGTTCGGTGATGGCATCGGATGCGTTCTTCCCGTTCCGC
GACGGCATCGATGCTGCGGCTGCGGCCGGCATCAAGGCAGTGATCCAGCCCGGCGGTTCGATGCGCGACGGCGAGGTGAT
CGCCGCCGCCGACGAACACGGCATCGCGATGGTGTTCACCGGCGTGCGCCACTTCCGGCATTGA

Upstream 100 bases:

>100_bases
TCCCTTCAATGCATAGATCGAGGCCATACGGAGTGTTGAGTCCATTCGAGCGCTGGCGCAGCCTACAATATCGGCTGCTC
CGCAACGACATCCCCCACCG

Downstream 100 bases:

>100_bases
TGTCACCGGCACAGGGATAGGTCGATGCAGCGTTTGCGTTGTTTTGCCGCGTGTCTTGCGACCGTCGTGCTGTTGGGCGC
GTGCAAGCAAGCGCCTGCGC

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MASDFLPVRRALLSVSDKTGLIDLARALVARNVELLSTGGTAKAIREAGLPVKDVAELTGFPEMMDGRVKTLHPLVHGGL
LGRAGVDEAVMAEHGIVPIDLLVLNLYPFESVTVKADCTLADAVENIDIGGPAMLRSAAKNFARVAVAADPAQYADLLVE
LEANDGQLSAAKRFALSVAAFNRVAQYDAAISNYLSAVADSAEAVPTRSPFPAQINSNFVKVMDLRYGENPHQSGAFYRD
LYPVPGTLATFQQLQGKELSYNNLADADAAWECVRQFDAPACVIVKHANPCGVAVGAGCGDAYELAYATDPTSAFGGILA
FNKTLDAATAKAILDRQFVEVLIAPDYEAGALEYATKKANVRVLKIPHGNGLNNYDTKRIGSGLLMQSADNRGMSQGELS
VVTQRAPNEAELGDLLFAWRVAKYVKSNAIVYAKDNRTIGVGAGQMSRVVSAKIAALKAEEAKLTVAGSVMASDAFFPFR
DGIDAAAAAGIKAVIQPGGSMRDGEVIAAADEHGIAMVFTGVRHFRH

Sequences:

>Translated_527_residues
MASDFLPVRRALLSVSDKTGLIDLARALVARNVELLSTGGTAKAIREAGLPVKDVAELTGFPEMMDGRVKTLHPLVHGGL
LGRAGVDEAVMAEHGIVPIDLLVLNLYPFESVTVKADCTLADAVENIDIGGPAMLRSAAKNFARVAVAADPAQYADLLVE
LEANDGQLSAAKRFALSVAAFNRVAQYDAAISNYLSAVADSAEAVPTRSPFPAQINSNFVKVMDLRYGENPHQSGAFYRD
LYPVPGTLATFQQLQGKELSYNNLADADAAWECVRQFDAPACVIVKHANPCGVAVGAGCGDAYELAYATDPTSAFGGILA
FNKTLDAATAKAILDRQFVEVLIAPDYEAGALEYATKKANVRVLKIPHGNGLNNYDTKRIGSGLLMQSADNRGMSQGELS
VVTQRAPNEAELGDLLFAWRVAKYVKSNAIVYAKDNRTIGVGAGQMSRVVSAKIAALKAEEAKLTVAGSVMASDAFFPFR
DGIDAAAAAGIKAVIQPGGSMRDGEVIAAADEHGIAMVFTGVRHFRH
>Mature_526_residues
ASDFLPVRRALLSVSDKTGLIDLARALVARNVELLSTGGTAKAIREAGLPVKDVAELTGFPEMMDGRVKTLHPLVHGGLL
GRAGVDEAVMAEHGIVPIDLLVLNLYPFESVTVKADCTLADAVENIDIGGPAMLRSAAKNFARVAVAADPAQYADLLVEL
EANDGQLSAAKRFALSVAAFNRVAQYDAAISNYLSAVADSAEAVPTRSPFPAQINSNFVKVMDLRYGENPHQSGAFYRDL
YPVPGTLATFQQLQGKELSYNNLADADAAWECVRQFDAPACVIVKHANPCGVAVGAGCGDAYELAYATDPTSAFGGILAF
NKTLDAATAKAILDRQFVEVLIAPDYEAGALEYATKKANVRVLKIPHGNGLNNYDTKRIGSGLLMQSADNRGMSQGELSV
VTQRAPNEAELGDLLFAWRVAKYVKSNAIVYAKDNRTIGVGAGQMSRVVSAKIAALKAEEAKLTVAGSVMASDAFFPFRD
GIDAAAAAGIKAVIQPGGSMRDGEVIAAADEHGIAMVFTGVRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family

Homologues:

Organism=Homo sapiens, GI20127454, Length=512, Percent_Identity=37.5, Blast_Score=266, Evalue=3e-71,
Organism=Escherichia coli, GI1790439, Length=524, Percent_Identity=60.4961832061069, Blast_Score=629, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=505, Percent_Identity=35.8415841584158, Blast_Score=257, Evalue=9e-69,
Organism=Caenorhabditis elegans, GI71985574, Length=350, Percent_Identity=28.2857142857143, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71985556, Length=119, Percent_Identity=36.1344537815126, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323768, Length=481, Percent_Identity=37.006237006237, Blast_Score=272, Evalue=7e-74,
Organism=Saccharomyces cerevisiae, GI6323056, Length=485, Percent_Identity=36.701030927835, Blast_Score=266, Evalue=4e-72,
Organism=Drosophila melanogaster, GI24649832, Length=507, Percent_Identity=39.4477317554241, Blast_Score=277, Evalue=2e-74,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR9_XANAC (Q8PQ19)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_640866.1
- ProteinModelPortal:   Q8PQ19
- SMR:   Q8PQ19
- GeneID:   1154584
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC0513
- NMPDR:   fig|190486.1.peg.510
- HOGENOM:   HBG498048
- OMA:   ASDGFFP
- ProtClustDB:   PRK00881
- BioCyc:   XAXO190486:XAC0513-MONOMER
- BRENDA:   2.1.2.3
- BRENDA:   3.5.4.10
- HAMAP:   MF_00139
- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607
- Gene3D:   G3DSA:3.40.50.1380
- PANTHER:   PTHR11692
- PIRSF:   PIRSF000414
- SMART:   SM00798
- SMART:   SM00851
- TIGRFAMs:   TIGR00355

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS; SSF53927 Cytidine_deaminase-like; SSF52335 MGS-like_dom

EC number: =2.1.2.3; =3.5.4.10

Molecular weight: Translated: 55726; Mature: 55595

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASDFLPVRRALLSVSDKTGLIDLARALVARNVELLSTGGTAKAIREAGLPVKDVAELTG
CCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHHCCCCHHHHHHHCC
FPEMMDGRVKTLHPLVHGGLLGRAGVDEAVMAEHGIVPIDLLVLNLYPFESVTVKADCTL
CHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHCCCCEEEEEEEEECCCCCEEEEECCCH
ADAVENIDIGGPAMLRSAAKNFARVAVAADPAQYADLLVELEANDGQLSAAKRFALSVAA
HHHHHCCCCCCHHHHHHHHHHHEEEEEECCHHHHEEEEEEEECCCCCHHHHHHHHHHHHH
FNRVAQYDAAISNYLSAVADSAEAVPTRSPFPAQINSNFVKVMDLRYGENPHQSGAFYRD
HHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEHC
LYPVPGTLATFQQLQGKELSYNNLADADAAWECVRQFDAPACVIVKHANPCGVAVGAGCG
CCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEEECCCC
DAYELAYATDPTSAFGGILAFNKTLDAATAKAILDRQFVEVLIAPDYEAGALEYATKKAN
CCEEEEEECCCCHHHCCCEEECCHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHCCCCC
VRVLKIPHGNGLNNYDTKRIGSGLLMQSADNRGMSQGELSVVTQRAPNEAELGDLLFAWR
EEEEEECCCCCCCCCCHHHHCCCCEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHH
VAKYVKSNAIVYAKDNRTIGVGAGQMSRVVSAKIAALKAEEAKLTVAGSVMASDAFFPFR
HHHHHHCCEEEEEECCCEEEECCHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCCCC
DGIDAAAAAGIKAVIQPGGSMRDGEVIAAADEHGIAMVFTGVRHFRH
CCCCHHHHHCHHEEECCCCCCCCCCEEEEECCCCEEEEEEHHHHHCC
>Mature Secondary Structure 
ASDFLPVRRALLSVSDKTGLIDLARALVARNVELLSTGGTAKAIREAGLPVKDVAELTG
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHHCCCCHHHHHHHCC
FPEMMDGRVKTLHPLVHGGLLGRAGVDEAVMAEHGIVPIDLLVLNLYPFESVTVKADCTL
CHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHCCCCEEEEEEEEECCCCCEEEEECCCH
ADAVENIDIGGPAMLRSAAKNFARVAVAADPAQYADLLVELEANDGQLSAAKRFALSVAA
HHHHHCCCCCCHHHHHHHHHHHEEEEEECCHHHHEEEEEEEECCCCCHHHHHHHHHHHHH
FNRVAQYDAAISNYLSAVADSAEAVPTRSPFPAQINSNFVKVMDLRYGENPHQSGAFYRD
HHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEHC
LYPVPGTLATFQQLQGKELSYNNLADADAAWECVRQFDAPACVIVKHANPCGVAVGAGCG
CCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEEECCCC
DAYELAYATDPTSAFGGILAFNKTLDAATAKAILDRQFVEVLIAPDYEAGALEYATKKAN
CCEEEEEECCCCHHHCCCEEECCHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHCCCCC
VRVLKIPHGNGLNNYDTKRIGSGLLMQSADNRGMSQGELSVVTQRAPNEAELGDLLFAWR
EEEEEECCCCCCCCCCHHHHCCCCEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHH
VAKYVKSNAIVYAKDNRTIGVGAGQMSRVVSAKIAALKAEEAKLTVAGSVMASDAFFPFR
HHHHHHCCEEEEEECCCEEEECCHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCCCC
DGIDAAAAAGIKAVIQPGGSMRDGEVIAAADEHGIAMVFTGVRHFRH
CCCCHHHHHCHHEEECCCCCCCCCCEEEEECCCCEEEEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217