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The map label for this gene is phaD [H]

Identifier: 21241233

GI number: 21241233

Start: 547869

End: 549413

Strand: Reverse

Name: phaD [H]

Synonym: XAC0460

Alternate gene names: 21241233

Gene position: 549413-547869 (Counterclockwise)

Preceding gene: 21241234

Following gene: 21241232

Centisome position: 10.62

GC content: 66.54

Gene sequence:

>1545_bases
ATGAACCATCTGCTGATCCTGCCCATCCTGATCCCGATGCTGGGCGCGTCGGCGTCGTTATTTGTCGAACACCGCCGCTA
TGGGCCACGCGTGCAGCGCAGCGTGGCCTGGGTCAGCATGGCCTTGTTGGCCGCAGCGGTCATCGCCTTGTTCGCACGCG
CTGCCGACGGCGAGATCCTGGTCTATCTGCTCGGCGACTGGCCGGCGCGCATCGGCATCGCGCTGCTGGGCGACCGCTTG
TCGGCGTGGATGCTGTTGACGACCTTGATCCTGGGTTCGGCGTGCCTGCTGCATGCATGCGCAGGCTGGGACCGGCGCGC
ACCGCATTTTCATGCGTTGTTCCAGTTCCAGCTGATGGGCTTGAACGGTGCGTTCCTGACCGGCGACATCTTCAATCTGT
TCGTGTTCTTCGAAGTGATGCTCATCGCCTCCTACGGCCTGCTGCTCAGCGGTGGGCGCGGGTTGCAGATGCGGGTGGGC
CTGCATTACGTGGTGTTCAACGTCTGCGCGTCGACGTTGTTCCTGATTGCGCTGGGCCTGCTGTTCGGTGTCTTCGGCAC
GCTCAACATGGCCGAGCTGTCGCAGCGCATTGCCGATCTGCCGGCGCAGGATGTGCCGTTGGCAAAGGCCACGCTGGGCT
TGTTGCTGCTGGTGTTCTGCAGCAAGGCCGCGTTGCTGCCGCTGTATCTGTGGCTGCCGGAAACCTATTCGCGCGCACCG
GCGGCGGTGGCCGCATTGTTCGCCATCATGACCAAGGTAGGGTTGTATGCAACGCTGCGGGTGTCCTCGTTGTGGTTTGG
CGCCGGTGCCGGCGCGCTGCACGGCTTCGGTCGCCATGCGCTGTTGTGGCTGGGCATCGCGACGCTGCTGATGGCCGCGT
TCGGGGTGATGGCCGCATCGCGGCTGCGGGTGATGGTGTCGTACCTGGTGGTGTTTTCGGCAGCGACCCTGTTCATCGCG
TTTTCGCTGGACGATGCCGGCGCACTGGGCGCAGGGCTGTATTACCTGCCGCACAGCAGCTTTGTGGCCGCTGCGTTGTT
CATGGTGTCGGACCTGATCCGCCGCCGCCGCGGCAGTGCCAGCGACCGCAAGGAAGTGGTGGCGCCACTGCCGGGGCGGG
CCATTCCAGGCACGATGTTCATGATTGCCGCGGTGGCGGTGGCCGGGTTGCCGCCGCTGCCCGGCTTTCTGGCCAAGGCG
GCGTTGCTGCAGCACGTGCCCCAGGGCCTGGTCGGGCCGGTGTGGGGCGCGGTGCTCGGCAGTAGCCTGCTGGTCGTGAT
CGGCCTCACCCGCGCCGGCGTGCGGTTGTTCTGGCGGGTGCCGGCCGCGGCGGAAAATGCGCCGGAACTGCAACCGCCGC
GACGTGGCCGCATGCGTCCGGTGGAGACCGCCGCCACGGTGCTGTTGCTAGGCGGGTTGGTTGCGATGACCGGCGCCGCA
GGTCCCTTGATGCATTACACCGATGCGGCCGCTGCGCAGTTGCGCGACCCCGGCGCGTATGTGGACCAGATACGCGCCAC
CACGCCGCAACGGAGGCAGCCATGA

Upstream 100 bases:

>100_bases
GCCTGGTGCTGGCGATCCGCAGCCGTAGCGACAACGGCAGCGACCACGTGGATGCGCACGAAGATGCCGCGACCGGCACG
GATCCGCGGCAGGCCCGGCG

Downstream 100 bases:

>100_bases
ACACGCGTCTGCCACTGCGTCGCCGCGTATTCCCATCGCCGCCGCTGAGCGTAACGGTCTTCGTCTTCTGGCTGCTGCTC
AGCGATAGCTTCGGCCCGCA

Product: monovalent cation/H+ antiporter subunit D

Products: NAD+; ubiquinol [C]

Alternate protein names: pH adaptation potassium efflux system protein D; Pha system subunit D [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MNHLLILPILIPMLGASASLFVEHRRYGPRVQRSVAWVSMALLAAAVIALFARAADGEILVYLLGDWPARIGIALLGDRL
SAWMLLTTLILGSACLLHACAGWDRRAPHFHALFQFQLMGLNGAFLTGDIFNLFVFFEVMLIASYGLLLSGGRGLQMRVG
LHYVVFNVCASTLFLIALGLLFGVFGTLNMAELSQRIADLPAQDVPLAKATLGLLLLVFCSKAALLPLYLWLPETYSRAP
AAVAALFAIMTKVGLYATLRVSSLWFGAGAGALHGFGRHALLWLGIATLLMAAFGVMAASRLRVMVSYLVVFSAATLFIA
FSLDDAGALGAGLYYLPHSSFVAAALFMVSDLIRRRRGSASDRKEVVAPLPGRAIPGTMFMIAAVAVAGLPPLPGFLAKA
ALLQHVPQGLVGPVWGAVLGSSLLVVIGLTRAGVRLFWRVPAAAENAPELQPPRRGRMRPVETAATVLLLGGLVAMTGAA
GPLMHYTDAAAAQLRDPGAYVDQIRATTPQRRQP

Sequences:

>Translated_514_residues
MNHLLILPILIPMLGASASLFVEHRRYGPRVQRSVAWVSMALLAAAVIALFARAADGEILVYLLGDWPARIGIALLGDRL
SAWMLLTTLILGSACLLHACAGWDRRAPHFHALFQFQLMGLNGAFLTGDIFNLFVFFEVMLIASYGLLLSGGRGLQMRVG
LHYVVFNVCASTLFLIALGLLFGVFGTLNMAELSQRIADLPAQDVPLAKATLGLLLLVFCSKAALLPLYLWLPETYSRAP
AAVAALFAIMTKVGLYATLRVSSLWFGAGAGALHGFGRHALLWLGIATLLMAAFGVMAASRLRVMVSYLVVFSAATLFIA
FSLDDAGALGAGLYYLPHSSFVAAALFMVSDLIRRRRGSASDRKEVVAPLPGRAIPGTMFMIAAVAVAGLPPLPGFLAKA
ALLQHVPQGLVGPVWGAVLGSSLLVVIGLTRAGVRLFWRVPAAAENAPELQPPRRGRMRPVETAATVLLLGGLVAMTGAA
GPLMHYTDAAAAQLRDPGAYVDQIRATTPQRRQP
>Mature_514_residues
MNHLLILPILIPMLGASASLFVEHRRYGPRVQRSVAWVSMALLAAAVIALFARAADGEILVYLLGDWPARIGIALLGDRL
SAWMLLTTLILGSACLLHACAGWDRRAPHFHALFQFQLMGLNGAFLTGDIFNLFVFFEVMLIASYGLLLSGGRGLQMRVG
LHYVVFNVCASTLFLIALGLLFGVFGTLNMAELSQRIADLPAQDVPLAKATLGLLLLVFCSKAALLPLYLWLPETYSRAP
AAVAALFAIMTKVGLYATLRVSSLWFGAGAGALHGFGRHALLWLGIATLLMAAFGVMAASRLRVMVSYLVVFSAATLFIA
FSLDDAGALGAGLYYLPHSSFVAAALFMVSDLIRRRRGSASDRKEVVAPLPGRAIPGTMFMIAAVAVAGLPPLPGFLAKA
ALLQHVPQGLVGPVWGAVLGSSLLVVIGLTRAGVRLFWRVPAAAENAPELQPPRRGRMRPVETAATVLLLGGLVAMTGAA
GPLMHYTDAAAAQLRDPGAYVDQIRATTPQRRQP

Specific function: Part of a K(+) efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Escherichia coli, GI1788613, Length=450, Percent_Identity=24.8888888888889, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004775
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 54767; Mature: 54767

Theoretical pI: Translated: 10.55; Mature: 10.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHLLILPILIPMLGASASLFVEHRRYGPRVQRSVAWVSMALLAAAVIALFARAADGEIL
CCCEEEHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
VYLLGDWPARIGIALLGDRLSAWMLLTTLILGSACLLHACAGWDRRAPHFHALFQFQLMG
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHEEC
LNGAFLTGDIFNLFVFFEVMLIASYGLLLSGGRGLQMRVGLHYVVFNVCASTLFLIALGL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
LFGVFGTLNMAELSQRIADLPAQDVPLAKATLGLLLLVFCSKAALLPLYLWLPETYSRAP
HHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
AAVAALFAIMTKVGLYATLRVSSLWFGAGAGALHGFGRHALLWLGIATLLMAAFGVMAAS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
RLRVMVSYLVVFSAATLFIAFSLDDAGALGAGLYYLPHSSFVAAALFMVSDLIRRRRGSA
HHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCC
SDRKEVVAPLPGRAIPGTMFMIAAVAVAGLPPLPGFLAKAALLQHVPQGLVGPVWGAVLG
CCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
SSLLVVIGLTRAGVRLFWRVPAAAENAPELQPPRRGRMRPVETAATVLLLGGLVAMTGAA
HHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
GPLMHYTDAAAAQLRDPGAYVDQIRATTPQRRQP
CCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNHLLILPILIPMLGASASLFVEHRRYGPRVQRSVAWVSMALLAAAVIALFARAADGEIL
CCCEEEHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
VYLLGDWPARIGIALLGDRLSAWMLLTTLILGSACLLHACAGWDRRAPHFHALFQFQLMG
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHEEC
LNGAFLTGDIFNLFVFFEVMLIASYGLLLSGGRGLQMRVGLHYVVFNVCASTLFLIALGL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
LFGVFGTLNMAELSQRIADLPAQDVPLAKATLGLLLLVFCSKAALLPLYLWLPETYSRAP
HHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
AAVAALFAIMTKVGLYATLRVSSLWFGAGAGALHGFGRHALLWLGIATLLMAAFGVMAAS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
RLRVMVSYLVVFSAATLFIAFSLDDAGALGAGLYYLPHSSFVAAALFMVSDLIRRRRGSA
HHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCC
SDRKEVVAPLPGRAIPGTMFMIAAVAVAGLPPLPGFLAKAALLQHVPQGLVGPVWGAVLG
CCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
SSLLVVIGLTRAGVRLFWRVPAAAENAPELQPPRRGRMRPVETAATVLLLGGLVAMTGAA
HHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
GPLMHYTDAAAAQLRDPGAYVDQIRATTPQRRQP
CCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9680201; 11481430 [H]