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The map label for this gene is gidA

Identifier: 21241151

GI number: 21241151

Start: 447779

End: 449683

Strand: Reverse

Name: gidA

Synonym: XAC0377

Alternate gene names: 21241151

Gene position: 449683-447779 (Counterclockwise)

Preceding gene: 21241152

Following gene: 21241150

Centisome position: 8.69

GC content: 67.56

Gene sequence:

>1905_bases
ATGTCCGACTCCTTTTATCGCTACGACGTCATCGTGATCGGAGGTGGTCATGCCGGCACCGAGGCCGCCCTTGCGTCTGC
ACGCGCAGGCGCGCGCACCTTGCTGCTCACCCACAACATCGAGACGGTGGGGGCGATGAGCTGTAACCCGGCCATCGGCG
GGATCGGCAAGGGCCACCTGGTCAAGGAGATCGACGCACTCGGCGGGGCGATGGCACGCGCAGCGGATCTGGCCGGCATC
CAGTGGCGCACGCTCAACGCCTCCAAGGGGCCGGCAGTGCGTGCCACGCGCTGCCAGGCCGACCGCAACCTGTACCGCAC
TGCGATCCGCTGCATCGTCGAGGCCCAGCCCAACCTGACCGTTTTCCAGGCGGCCGTGGACGATCTGATCATTCATCACG
GCGCCAGCGAAGCCGACAGCGTGCGTGGGGTCATCACCCAGACCGGACTGCGTTTCCAAGCGCCTTCGGTGGTGCTGACG
GCCGGCACGTTCCTGGCCGGCAAGATCCATGTCGGCCAAACGCAGTACGCGGCCGGGCGCATGGGCGATCCGCCGGCCAC
CACGCTGGCCGCGCGCCTGCGCGAGCGCCCGTTCGCGATCGACCGGCTCAAGACCGGCACCCCGCCGCGGATCGACGGGC
GCACCCTCGATTACGGCGTGATGGCCGAGCAGCCCGGCGACGACCCATTGCCGGTGATGTCGTTCATGGGCCAGGTCAGC
GACCATCCGCGCCAGGTCAGCTGCTGGATCACCCAGACCACCGAGCAAACCCACGAGATCATTCGCAACGCGCTGCATCG
CTCGCCGCTGTATTCGGGGCAGATCGAAGGCATCGGCCCGCGCTACTGCCCGTCGATCGAAGACAAGGTGGTGCGTTTTG
CCGAGAAGACCAGCCACCAGATCTTCGTCGAACCCGAAGGCCTGGAAGTGGCCGAGATCTATCCCAACGGTATTTCCACC
TCGCTGCCGTTCGATGTGCAGCTGGCGCTGGTGCGCTCGATCCACGGCTTTGCGAACGCGCACATCACCCGCCCCGGCTA
CGCCATCGAGTACGACTTCTTCGACCCGCGCGGGCTCAAGGCCTCGCTGGAGACCAAGGCGGTGGGCGGGCTGTTCTTCG
CCGGGCAGATCAACGGGACCACCGGTTACGAAGAGGCGGCCGCCCAGGGTCTGCTCGCCGGGCTCAACGCAGCGCGCCAC
GTGCAGGGGCTGCCGGCGTGGTCGCCGCGCCGCGATGAGGCCTACCTGGGCGTGCTGGTGGACGATCTCATTACCCACGG
CACCACCGAGCCGTACCGCATGTTCACCAGCCGCGCCGAATACCGATTGCAGCTGCGCGAGGACAATGCCGATGCGCGCC
TGACCGGCGTCGGGCGTGCGATGGGCCTGGTGGACGACGCGCGCTGGGCGCGCTTTGCGGCCAAGCAGGAGGCCGTGCAA
CGCGAAACGGCGCGGCTGTCTGCACTGTGGGCCACACCGGGCAATGCGCTGGGCCGCGAGGTGGCCGACGCGCTGGGCGT
GACGGTCAGCCGCGAAACCAACGTGCTGGATCTGATCAAGCGCCCGGAACTCACCTACGCCACCTTGATGCGGGTGCCGA
CCCTGGGCCCGGGCGTGGACGATGCGCAGGTGGCCGAGCAGGTCGAGATCAGCGTCAAGTACGCCGGTTATCTGGATCGC
CAGCGCGACGATATCGCGCGTCAGCAACGTCACGAAACCACGCCGATCCCGGACGGTTTCGATTACGCCAGCGTGCGCGG
CCTGTCGATCGAAGTGCAGCAGAAACTTGAACGCGTGCGCCCCCAGCACATCGGTCAGGCACAACGGATTCCCGGCATGA
CCCCGGCGGCGATCTCGCTGCTGCTGGTGCATCTGGAGCGCGCGCGACGCAGCCAAGTGGCGTGA

Upstream 100 bases:

>100_bases
TGTGATTTGAATCACATTTTCTACCGAACAAGTGACCTGGCCCGCCCTTCAGCCGATACTTCCGCGGCCGTTTTCCCTTT
TCCGCTCCTGGTCCACGCTC

Downstream 100 bases:

>100_bases
TCGACGCGACGGCCCTGCCCGTCGCCAGTGATCGTTGGCATCCATCCCTCTGACGACAGCGGGCGACCGCGCCGGTCATC
GCGACACGATGGCGTGTCCC

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 634; Mature: 633

Protein sequence:

>634_residues
MSDSFYRYDVIVIGGGHAGTEAALASARAGARTLLLTHNIETVGAMSCNPAIGGIGKGHLVKEIDALGGAMARAADLAGI
QWRTLNASKGPAVRATRCQADRNLYRTAIRCIVEAQPNLTVFQAAVDDLIIHHGASEADSVRGVITQTGLRFQAPSVVLT
AGTFLAGKIHVGQTQYAAGRMGDPPATTLAARLRERPFAIDRLKTGTPPRIDGRTLDYGVMAEQPGDDPLPVMSFMGQVS
DHPRQVSCWITQTTEQTHEIIRNALHRSPLYSGQIEGIGPRYCPSIEDKVVRFAEKTSHQIFVEPEGLEVAEIYPNGIST
SLPFDVQLALVRSIHGFANAHITRPGYAIEYDFFDPRGLKASLETKAVGGLFFAGQINGTTGYEEAAAQGLLAGLNAARH
VQGLPAWSPRRDEAYLGVLVDDLITHGTTEPYRMFTSRAEYRLQLREDNADARLTGVGRAMGLVDDARWARFAAKQEAVQ
RETARLSALWATPGNALGREVADALGVTVSRETNVLDLIKRPELTYATLMRVPTLGPGVDDAQVAEQVEISVKYAGYLDR
QRDDIARQQRHETTPIPDGFDYASVRGLSIEVQQKLERVRPQHIGQAQRIPGMTPAAISLLLVHLERARRSQVA

Sequences:

>Translated_634_residues
MSDSFYRYDVIVIGGGHAGTEAALASARAGARTLLLTHNIETVGAMSCNPAIGGIGKGHLVKEIDALGGAMARAADLAGI
QWRTLNASKGPAVRATRCQADRNLYRTAIRCIVEAQPNLTVFQAAVDDLIIHHGASEADSVRGVITQTGLRFQAPSVVLT
AGTFLAGKIHVGQTQYAAGRMGDPPATTLAARLRERPFAIDRLKTGTPPRIDGRTLDYGVMAEQPGDDPLPVMSFMGQVS
DHPRQVSCWITQTTEQTHEIIRNALHRSPLYSGQIEGIGPRYCPSIEDKVVRFAEKTSHQIFVEPEGLEVAEIYPNGIST
SLPFDVQLALVRSIHGFANAHITRPGYAIEYDFFDPRGLKASLETKAVGGLFFAGQINGTTGYEEAAAQGLLAGLNAARH
VQGLPAWSPRRDEAYLGVLVDDLITHGTTEPYRMFTSRAEYRLQLREDNADARLTGVGRAMGLVDDARWARFAAKQEAVQ
RETARLSALWATPGNALGREVADALGVTVSRETNVLDLIKRPELTYATLMRVPTLGPGVDDAQVAEQVEISVKYAGYLDR
QRDDIARQQRHETTPIPDGFDYASVRGLSIEVQQKLERVRPQHIGQAQRIPGMTPAAISLLLVHLERARRSQVA
>Mature_633_residues
SDSFYRYDVIVIGGGHAGTEAALASARAGARTLLLTHNIETVGAMSCNPAIGGIGKGHLVKEIDALGGAMARAADLAGIQ
WRTLNASKGPAVRATRCQADRNLYRTAIRCIVEAQPNLTVFQAAVDDLIIHHGASEADSVRGVITQTGLRFQAPSVVLTA
GTFLAGKIHVGQTQYAAGRMGDPPATTLAARLRERPFAIDRLKTGTPPRIDGRTLDYGVMAEQPGDDPLPVMSFMGQVSD
HPRQVSCWITQTTEQTHEIIRNALHRSPLYSGQIEGIGPRYCPSIEDKVVRFAEKTSHQIFVEPEGLEVAEIYPNGISTS
LPFDVQLALVRSIHGFANAHITRPGYAIEYDFFDPRGLKASLETKAVGGLFFAGQINGTTGYEEAAAQGLLAGLNAARHV
QGLPAWSPRRDEAYLGVLVDDLITHGTTEPYRMFTSRAEYRLQLREDNADARLTGVGRAMGLVDDARWARFAAKQEAVQR
ETARLSALWATPGNALGREVADALGVTVSRETNVLDLIKRPELTYATLMRVPTLGPGVDDAQVAEQVEISVKYAGYLDRQ
RDDIARQQRHETTPIPDGFDYASVRGLSIEVQQKLERVRPQHIGQAQRIPGMTPAAISLLLVHLERARRSQVA

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=640, Percent_Identity=44.0625, Blast_Score=482, Evalue=1e-136,
Organism=Homo sapiens, GI19882217, Length=665, Percent_Identity=42.406015037594, Blast_Score=467, Evalue=1e-131,
Organism=Homo sapiens, GI183227703, Length=680, Percent_Identity=41.4705882352941, Blast_Score=462, Evalue=1e-130,
Organism=Escherichia coli, GI2367273, Length=620, Percent_Identity=64.6774193548387, Blast_Score=817, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=624, Percent_Identity=39.7435897435897, Blast_Score=452, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6321202, Length=644, Percent_Identity=44.0993788819876, Blast_Score=494, Evalue=1e-140,
Organism=Drosophila melanogaster, GI24658174, Length=632, Percent_Identity=45.0949367088608, Blast_Score=486, Evalue=1e-137,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_XANAC (Q8PQE8)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_640733.1
- ProteinModelPortal:   Q8PQE8
- SMR:   Q8PQE8
- GeneID:   1154448
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC0377
- NMPDR:   fig|190486.1.peg.377
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- ProtClustDB:   PRK05192
- BioCyc:   XAXO190486:XAC0377-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 68882; Mature: 68751

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSFYRYDVIVIGGGHAGTEAALASARAGARTLLLTHNIETVGAMSCNPAIGGIGKGHL
CCCCEEEEEEEEEECCCCCHHHHHHHHCCCCEEEEEEECCCEECCCCCCCCCCCCCCCHH
VKEIDALGGAMARAADLAGIQWRTLNASKGPAVRATRCQADRNLYRTAIRCIVEAQPNLT
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCE
VFQAAVDDLIIHHGASEADSVRGVITQTGLRFQAPSVVLTAGTFLAGKIHVGQTQYAAGR
EHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEECCCEEEEECCEEEEEEEECCCHHCCCC
MGDPPATTLAARLRERPFAIDRLKTGTPPRIDGRTLDYGVMAEQPGDDPLPVMSFMGQVS
CCCCHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCEECCCEEECCCCCCCCHHHHHHHHHC
DHPRQVSCWITQTTEQTHEIIRNALHRSPLYSGQIEGIGPRYCPSIEDKVVRFAEKTSHQ
CCCCEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCE
IFVEPEGLEVAEIYPNGISTSLPFDVQLALVRSIHGFANAHITRPGYAIEYDFFDPRGLK
EEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEECCCEEEEECCCCCCCCC
ASLETKAVGGLFFAGQINGTTGYEEAAAQGLLAGLNAARHVQGLPAWSPRRDEAYLGVLV
CCCCHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
DDLITHGTTEPYRMFTSRAEYRLQLREDNADARLTGVGRAMGLVDDARWARFAAKQEAVQ
HHHHHCCCCCHHHHHHCCCEEEEEEECCCCCEEEEECHHHHCCHHHHHHHHHHHHHHHHH
RETARLSALWATPGNALGREVADALGVTVSRETNVLDLIKRPELTYATLMRVPTLGPGVD
HHHHHHEEEECCCCHHHHHHHHHHHCCEECCCCCHHHHHCCCCCHHHHHHHCCCCCCCCC
DAQVAEQVEISVKYAGYLDRQRDDIARQQRHETTPIPDGFDYASVRGLSIEVQQKLERVR
HHHHHHHEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCHHEECCEEHHHHHHHHHC
PQHIGQAQRIPGMTPAAISLLLVHLERARRSQVA
HHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDSFYRYDVIVIGGGHAGTEAALASARAGARTLLLTHNIETVGAMSCNPAIGGIGKGHL
CCCEEEEEEEEEECCCCCHHHHHHHHCCCCEEEEEEECCCEECCCCCCCCCCCCCCCHH
VKEIDALGGAMARAADLAGIQWRTLNASKGPAVRATRCQADRNLYRTAIRCIVEAQPNLT
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCE
VFQAAVDDLIIHHGASEADSVRGVITQTGLRFQAPSVVLTAGTFLAGKIHVGQTQYAAGR
EHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEECCCEEEEECCEEEEEEEECCCHHCCCC
MGDPPATTLAARLRERPFAIDRLKTGTPPRIDGRTLDYGVMAEQPGDDPLPVMSFMGQVS
CCCCHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCEECCCEEECCCCCCCCHHHHHHHHHC
DHPRQVSCWITQTTEQTHEIIRNALHRSPLYSGQIEGIGPRYCPSIEDKVVRFAEKTSHQ
CCCCEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCE
IFVEPEGLEVAEIYPNGISTSLPFDVQLALVRSIHGFANAHITRPGYAIEYDFFDPRGLK
EEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEECCCEEEEECCCCCCCCC
ASLETKAVGGLFFAGQINGTTGYEEAAAQGLLAGLNAARHVQGLPAWSPRRDEAYLGVLV
CCCCHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
DDLITHGTTEPYRMFTSRAEYRLQLREDNADARLTGVGRAMGLVDDARWARFAAKQEAVQ
HHHHHCCCCCHHHHHHCCCEEEEEEECCCCCEEEEECHHHHCCHHHHHHHHHHHHHHHHH
RETARLSALWATPGNALGREVADALGVTVSRETNVLDLIKRPELTYATLMRVPTLGPGVD
HHHHHHEEEECCCCHHHHHHHHHHHCCEECCCCCHHHHHCCCCCHHHHHHHCCCCCCCCC
DAQVAEQVEISVKYAGYLDRQRDDIARQQRHETTPIPDGFDYASVRGLSIEVQQKLERVR
HHHHHHHEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCHHEECCEEHHHHHHHHHC
PQHIGQAQRIPGMTPAAISLLLVHLERARRSQVA
HHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217