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The map label for this gene is ynfL [H]

Identifier: 21241090

GI number: 21241090

Start: 377525

End: 378412

Strand: Reverse

Name: ynfL [H]

Synonym: XAC0316

Alternate gene names: 21241090

Gene position: 378412-377525 (Counterclockwise)

Preceding gene: 21241092

Following gene: 21241088

Centisome position: 7.31

GC content: 67.68

Gene sequence:

>888_bases
ATGGAGTTGCGCCACCTGCGTTATTTCCTGGCGGTTGCCGAAGAAGGCAACTTCACCCGCGCCGCCGCGCGCGTGGGCAT
TGGCCAACCGCCGCTCAGCCAGCAGATCCAGACCCTGGAACGCGAGCTGGGCACGCCATTGTTTCGGCGCACGCACAGCG
GCGCCGAACTCACTGCGGCTGGCGAGGCGTTTTTGGGCGAGGTGCGCCGCGTACTTGCCGATGTGGAACGCGCCGCCGAA
ACCGCACGCCGGGTGGCGCGCGGCGAATCCGGACGGTTGCGCCTGGGATTCACCGCCTCTGCCGCCTTCAGCCCGGTGGT
GCCGCGCCTGATTCGCGACTTCCGCCGCAAATGGCCGCAGGTCGAATTGCTGCTGGAAGAGACCAATACCGCCAGCCTGC
TGGTCGGGCTGGCCGATGGCCGTCTGGATGCAGCGTTCGTGCGCTATGGGCTGAGCACGCCAGCGGAATTGCAGTTGCTG
CGCTTTGCCGACGAGCCGATGAAGATCGCCGTGCCGGCCGCGCACCCCCTGGCCACGCGCAACAGCGCGCCGTTGGCGGC
GCTGGCCGGCGAGCCCTTTATCCTGTTTCCGCGCAGTTTCGGCTCGAGCCTGTACGACGAAATCCTCGCCGCCTGCCGCG
ACTCCGGCGTGACCTTGCGCATCACCCAGGAGGCGCCGCAGATGTCGTCCATCATCAATCTGGTAGCCGCCGAGCTGGGG
GTTTCGGTGGTGCCCGCCTCCACCGCGCAGCTGCAGCGGCCCGGCGTGCGCTATCTGGATATCGAAGGCCATATGCCGCT
GGCGCGACTGGCCCTGGCCGCCGCGCCGGGTGGATTGGAAACCACACCGTTGGTGCGGCATTTGTGGGCGCTGGCAAAGT
TGCTCTAG

Upstream 100 bases:

>100_bases
ACACAGCAGTGCACGTGCGACGTGGGAAGGCGAGTATTCGCCTGCCGTCACCCCCTGTCCAATATATGATGGTGGCAGCT
GCCATATCTGGAACAGATGG

Downstream 100 bases:

>100_bases
CGCTGGCCACACGACCTACGATACAGCGACATTCTTGCTTGATGCCGTTGGACGATGTTGATCCTGGGCCCCGGTCATTG
CCGACCGCCTGCACTGGCCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MELRHLRYFLAVAEEGNFTRAAARVGIGQPPLSQQIQTLERELGTPLFRRTHSGAELTAAGEAFLGEVRRVLADVERAAE
TARRVARGESGRLRLGFTASAAFSPVVPRLIRDFRRKWPQVELLLEETNTASLLVGLADGRLDAAFVRYGLSTPAELQLL
RFADEPMKIAVPAAHPLATRNSAPLAALAGEPFILFPRSFGSSLYDEILAACRDSGVTLRITQEAPQMSSIINLVAAELG
VSVVPASTAQLQRPGVRYLDIEGHMPLARLALAAAPGGLETTPLVRHLWALAKLL

Sequences:

>Translated_295_residues
MELRHLRYFLAVAEEGNFTRAAARVGIGQPPLSQQIQTLERELGTPLFRRTHSGAELTAAGEAFLGEVRRVLADVERAAE
TARRVARGESGRLRLGFTASAAFSPVVPRLIRDFRRKWPQVELLLEETNTASLLVGLADGRLDAAFVRYGLSTPAELQLL
RFADEPMKIAVPAAHPLATRNSAPLAALAGEPFILFPRSFGSSLYDEILAACRDSGVTLRITQEAPQMSSIINLVAAELG
VSVVPASTAQLQRPGVRYLDIEGHMPLARLALAAAPGGLETTPLVRHLWALAKLL
>Mature_295_residues
MELRHLRYFLAVAEEGNFTRAAARVGIGQPPLSQQIQTLERELGTPLFRRTHSGAELTAAGEAFLGEVRRVLADVERAAE
TARRVARGESGRLRLGFTASAAFSPVVPRLIRDFRRKWPQVELLLEETNTASLLVGLADGRLDAAFVRYGLSTPAELQLL
RFADEPMKIAVPAAHPLATRNSAPLAALAGEPFILFPRSFGSSLYDEILAACRDSGVTLRITQEAPQMSSIINLVAAELG
VSVVPASTAQLQRPGVRYLDIEGHMPLARLALAAAPGGLETTPLVRHLWALAKLL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=288, Percent_Identity=43.0555555555556, Blast_Score=211, Evalue=6e-56,
Organism=Escherichia coli, GI2367136, Length=265, Percent_Identity=33.2075471698113, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1788887, Length=293, Percent_Identity=33.4470989761092, Blast_Score=140, Evalue=9e-35,
Organism=Escherichia coli, GI1790399, Length=280, Percent_Identity=32.1428571428571, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI157672245, Length=258, Percent_Identity=31.0077519379845, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1788297, Length=147, Percent_Identity=34.0136054421769, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI87081904, Length=184, Percent_Identity=32.0652173913043, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788748, Length=244, Percent_Identity=25.8196721311475, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1787530, Length=248, Percent_Identity=28.2258064516129, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI145693105, Length=244, Percent_Identity=26.6393442622951, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1789506, Length=272, Percent_Identity=23.1617647058824, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787601, Length=247, Percent_Identity=26.7206477732794, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787806, Length=184, Percent_Identity=32.6086956521739, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI87082132, Length=254, Percent_Identity=28.740157480315, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1790262, Length=251, Percent_Identity=25.8964143426295, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789204, Length=254, Percent_Identity=27.5590551181102, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790208, Length=151, Percent_Identity=34.4370860927152, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787589, Length=172, Percent_Identity=32.5581395348837, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787949, Length=140, Percent_Identity=34.2857142857143, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786401, Length=154, Percent_Identity=29.2207792207792, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788481, Length=202, Percent_Identity=27.2277227722772, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31939; Mature: 31939

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRHLRYFLAVAEEGNFTRAAARVGIGQPPLSQQIQTLERELGTPLFRRTHSGAELTAA
CCHHHHHHHHEECCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHCCCCCCEEHH
GEAFLGEVRRVLADVERAAETARRVARGESGRLRLGFTASAAFSPVVPRLIRDFRRKWPQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCC
VELLLEETNTASLLVGLADGRLDAAFVRYGLSTPAELQLLRFADEPMKIAVPAAHPLATR
EEEEEECCCCEEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHCCCCCEEEECCCCCCCCC
NSAPLAALAGEPFILFPRSFGSSLYDEILAACRDSGVTLRITQEAPQMSSIINLVAAELG
CCCCEEEECCCCEEEECHHHCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHC
VSVVPASTAQLQRPGVRYLDIEGHMPLARLALAAAPGGLETTPLVRHLWALAKLL
CEEECCCHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHC
>Mature Secondary Structure
MELRHLRYFLAVAEEGNFTRAAARVGIGQPPLSQQIQTLERELGTPLFRRTHSGAELTAA
CCHHHHHHHHEECCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHCCCCCCEEHH
GEAFLGEVRRVLADVERAAETARRVARGESGRLRLGFTASAAFSPVVPRLIRDFRRKWPQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCC
VELLLEETNTASLLVGLADGRLDAAFVRYGLSTPAELQLLRFADEPMKIAVPAAHPLATR
EEEEEECCCCEEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHCCCCCEEEECCCCCCCCC
NSAPLAALAGEPFILFPRSFGSSLYDEILAACRDSGVTLRITQEAPQMSSIINLVAAELG
CCCCEEEECCCCEEEECHHHCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHC
VSVVPASTAQLQRPGVRYLDIEGHMPLARLALAAAPGGLETTPLVRHLWALAKLL
CEEECCCHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]