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The map label for this gene is vanB [H]

Identifier: 21241084

GI number: 21241084

Start: 371027

End: 371986

Strand: Reverse

Name: vanB [H]

Synonym: XAC0310

Alternate gene names: 21241084

Gene position: 371986-371027 (Counterclockwise)

Preceding gene: 21241085

Following gene: 21241083

Centisome position: 7.19

GC content: 68.02

Gene sequence:

>960_bases
ATGAGCCTGCATGAAGTGCGCGTTGCCGACGTCGTCGATCAGGGTCACCGCCAGCGCGCGATTCGGCTGGAACCGGTGGG
CGCGCAATTGCCGGCATTCGAGGCCGGCGCGCATGTCGACCTGCATCTGCCGGATGGCTTGATTCGCCAGTACTCGATCG
CCAGCGCGCCGCATGTGCGCGACCACTATCTGCTGTGCGTCAAGCTGGCCGACGCCTCGCGCGGTGGGTCGCGGCATCTG
TGCGAGCAGCTTGCGCCCGGCGACCGCCTGCGCATCTCCACACCGCGCAATCTGTTCCCGCTGCACCCGGGCGAGCGCCA
CGTGCTGCTGGCCGCTGGCATCGGGATCACCCCGCTGCTGTCGATGGCCGAAGCACTGGAAGCGCGCGGCGAGCCCTTCG
TGCTGCATTACTACGCACGCCGGCATGCCGATGTGGCGTTTTCGCAGCGGCTGCAACAAGGCTTTACGCATGGGCAGGTG
TCCCTGCATCTGAGCGATGGCGGGCAGAGCCCACGCGTGCATGTGCCCGAGGAACTGGGCCAGGCGCGTGCATGCGATCA
GCTGTATCTGTGCGGGCCGGCGGCGTTCATGGACCACTTCACCGTGCTGGCGAAGGCGCATGGCTGGACGGCGGCGCAAC
TGCATCGCGAACACTTCGCCGCCCCCGCGCCCAGCCTTGCCCACGATGCCGACAGCGCGTTCGAGGTGGAACTGGCCGCC
AGCGGCCGCGTGGTGCAGGTGCCGGCCGACTGCAGCATCGCCAGTGCCCTGCTCGATGCCGGTATCGAGGTGCCGCTGTC
GTGCGAACAAGGCATGTGTGGCGCCTGTCTGACCGGCGTGCTGGCTGGCACGCCAGATCACCGCGACAGCGTGCTCAGCG
ACGCCGAACGCGCCACCAATCGGCAGATCACCGTGTGCTGTTCGCGCAGCCGCTCGCCGCGGTTGGTGCTGGATCTTTGA

Upstream 100 bases:

>100_bases
TGCATATTCCCGCCGATCGCAGTTCCATCGCGTATCGCCGCGGGCTGCGCAGCCAGGGCTATAGCGACTTCTTCCTGCGC
TGACGGCCGAGGACACCCGC

Downstream 100 bases:

>100_bases
ACGCTGTTTCCGCGGCGCCACTGCAACTGTTCAGTCACCTTGCGCAGCCTGACGCCTCGCTTGCCTTTCACGATGGCGGG
CAACAGAGCGCGCCATCGCG

Product: vanillate O-demethylase oxidoreductase

Products: 3,4-dihydroxybenzoate; NAD+; H2O; formaldehyde

Alternate protein names: Vanillate degradation ferredoxin-like protein [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MSLHEVRVADVVDQGHRQRAIRLEPVGAQLPAFEAGAHVDLHLPDGLIRQYSIASAPHVRDHYLLCVKLADASRGGSRHL
CEQLAPGDRLRISTPRNLFPLHPGERHVLLAAGIGITPLLSMAEALEARGEPFVLHYYARRHADVAFSQRLQQGFTHGQV
SLHLSDGGQSPRVHVPEELGQARACDQLYLCGPAAFMDHFTVLAKAHGWTAAQLHREHFAAPAPSLAHDADSAFEVELAA
SGRVVQVPADCSIASALLDAGIEVPLSCEQGMCGACLTGVLAGTPDHRDSVLSDAERATNRQITVCCSRSRSPRLVLDL

Sequences:

>Translated_319_residues
MSLHEVRVADVVDQGHRQRAIRLEPVGAQLPAFEAGAHVDLHLPDGLIRQYSIASAPHVRDHYLLCVKLADASRGGSRHL
CEQLAPGDRLRISTPRNLFPLHPGERHVLLAAGIGITPLLSMAEALEARGEPFVLHYYARRHADVAFSQRLQQGFTHGQV
SLHLSDGGQSPRVHVPEELGQARACDQLYLCGPAAFMDHFTVLAKAHGWTAAQLHREHFAAPAPSLAHDADSAFEVELAA
SGRVVQVPADCSIASALLDAGIEVPLSCEQGMCGACLTGVLAGTPDHRDSVLSDAERATNRQITVCCSRSRSPRLVLDL
>Mature_318_residues
SLHEVRVADVVDQGHRQRAIRLEPVGAQLPAFEAGAHVDLHLPDGLIRQYSIASAPHVRDHYLLCVKLADASRGGSRHLC
EQLAPGDRLRISTPRNLFPLHPGERHVLLAAGIGITPLLSMAEALEARGEPFVLHYYARRHADVAFSQRLQQGFTHGQVS
LHLSDGGQSPRVHVPEELGQARACDQLYLCGPAAFMDHFTVLAKAHGWTAAQLHREHFAAPAPSLAHDADSAFEVELAAS
GRVVQVPADCSIASALLDAGIEVPLSCEQGMCGACLTGVLAGTPDHRDSVLSDAERATNRQITVCCSRSRSPRLVLDL

Specific function: Unknown

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=298, Percent_Identity=38.5906040268456, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1787098, Length=279, Percent_Identity=27.5985663082437, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787658, Length=282, Percent_Identity=29.0780141843972, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.14.13.82

Molecular weight: Translated: 34399; Mature: 34268

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLHEVRVADVVDQGHRQRAIRLEPVGAQLPAFEAGAHVDLHLPDGLIRQYSIASAPHVR
CCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHCCCCCCCC
DHYLLCVKLADASRGGSRHLCEQLAPGDRLRISTPRNLFPLHPGERHVLLAAGIGITPLL
CCEEEEEEEECCCCCCHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEEEEECCCHHHHH
SMAEALEARGEPFVLHYYARRHADVAFSQRLQQGFTHGQVSLHLSDGGQSPRVHVPEELG
HHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEECCHHHH
QARACDQLYLCGPAAFMDHFTVLAKAHGWTAAQLHREHFAAPAPSLAHDADSAFEVELAA
HHHCCCEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHCCCCCCEEEEEEC
SGRVVQVPADCSIASALLDAGIEVPLSCEQGMCGACLTGVLAGTPDHRDSVLSDAERATN
CCCEEEECCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCC
RQITVCCSRSRSPRLVLDL
CEEEEEECCCCCCEEEEEC
>Mature Secondary Structure 
SLHEVRVADVVDQGHRQRAIRLEPVGAQLPAFEAGAHVDLHLPDGLIRQYSIASAPHVR
CCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHCCCCCCCC
DHYLLCVKLADASRGGSRHLCEQLAPGDRLRISTPRNLFPLHPGERHVLLAAGIGITPLL
CCEEEEEEEECCCCCCHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEEEEECCCHHHHH
SMAEALEARGEPFVLHYYARRHADVAFSQRLQQGFTHGQVSLHLSDGGQSPRVHVPEELG
HHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEECCHHHH
QARACDQLYLCGPAAFMDHFTVLAKAHGWTAAQLHREHFAAPAPSLAHDADSAFEVELAA
HHHCCCEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHCCCCCCEEEEEEC
SGRVVQVPADCSIASALLDAGIEVPLSCEQGMCGACLTGVLAGTPDHRDSVLSDAERATN
CCCEEEECCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCC
RQITVCCSRSRSPRLVLDL
CEEEEEECCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: vanillate; O2; NADH; H+

Specific reaction: vanillate + O2 + NADH + H+ = 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9098058 [H]