Click here to switch to the map view.

The map label for this gene is pucL [H]

Identifier: 21241071

GI number: 21241071

Start: 354749

End: 355240

Strand: Reverse

Name: pucL [H]

Synonym: XAC0297

Alternate gene names: 21241071

Gene position: 355240-354749 (Counterclockwise)

Preceding gene: 21241072

Following gene: 21241070

Centisome position: 6.86

GC content: 65.24

Gene sequence:

>492_bases
ATGAGCGTGGTCGACGACCCCGGGCTGGACGATGCCGCCTTCATGGCGCGCTACCGCGACTTGTTCGAGCACTCGCCGTG
GGTGGTGGAACGTGCGCTGCGTCGGCGGCCGTTCGTCGATGTGCATCGCGGCCTGATGCAGGTTGTCTACGACGCATCGC
CGCAAGATCAGGTCGCACTGATCCGCGCCCACCCCGAGCTGGCCGGCAAGGCCGCGATCGACCGCACGCTCACTGCCGCA
TCGGCCGCAGAGCAGGCTTCGGCAGGGCTGGACCGGCTCGACGAAGACGAATTCAAGCGCTTCCACGCGCTCAATCAGGC
CTATCGCCACCGTTTCGGATTTCCGTTCGTGATCTGCGTGCGGCTGCACGACAAGGCCGGCATTCTGGCCGCGCTGGAGC
AGCGTCTGGATGCATCGCGCAATGCAGAGATCGCCACCGCCATCGCTGAAATCGGCAAGATCGTTCACCTGCGCCTGGAG
GCACTGTCATGA

Upstream 100 bases:

>100_bases
AGGGCAGCAGTGCGCGCGGTCGGCAGAGCCAGAGCTGGGTGCGCTTTGCCGACGGCTGGAAGGTGGTGGCCGCGCATGTC
TCGCTGCAAGGCACGCACGC

Downstream 100 bases:

>100_bases
GCCTGGCCCACCTCGAACGTACCCTCGCCCACGACCTGCAATGCCTGGCCCACGGCGGCGATGCCTGGGTGCAGCCGCGC
GTGCATCCGAGCGGGCACGT

Product: hypothetical protein

Products: NA

Alternate protein names: 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase; OHCU decarboxylase; Uricase; Urate oxidase [H]

Number of amino acids: Translated: 163; Mature: 162

Protein sequence:

>163_residues
MSVVDDPGLDDAAFMARYRDLFEHSPWVVERALRRRPFVDVHRGLMQVVYDASPQDQVALIRAHPELAGKAAIDRTLTAA
SAAEQASAGLDRLDEDEFKRFHALNQAYRHRFGFPFVICVRLHDKAGILAALEQRLDASRNAEIATAIAEIGKIVHLRLE
ALS

Sequences:

>Translated_163_residues
MSVVDDPGLDDAAFMARYRDLFEHSPWVVERALRRRPFVDVHRGLMQVVYDASPQDQVALIRAHPELAGKAAIDRTLTAA
SAAEQASAGLDRLDEDEFKRFHALNQAYRHRFGFPFVICVRLHDKAGILAALEQRLDASRNAEIATAIAEIGKIVHLRLE
ALS
>Mature_162_residues
SVVDDPGLDDAAFMARYRDLFEHSPWVVERALRRRPFVDVHRGLMQVVYDASPQDQVALIRAHPELAGKAAIDRTLTAAS
AAEQASAGLDRLDEDEFKRFHALNQAYRHRFGFPFVICVRLHDKAGILAALEQRLDASRNAEIATAIAEIGKIVHLRLEA
LS

Specific function: Catalyzes two steps in the degradation of uric acid, i.e. the oxidation of uric acid to 5-hydroxyisourate (HIU) and the stereoselective decarboxylation of 2-oxo-4-hydroxy-4-carboxy-5- ureidoimidazoline (OHCU) to (S)-allantoin [H]

COG id: COG3195

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the uricase family [H]

Homologues:

Organism=Homo sapiens, GI157743280, Length=162, Percent_Identity=33.9506172839506, Blast_Score=89, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018020
- InterPro:   IPR017580
- InterPro:   IPR002042
- InterPro:   IPR019842 [H]

Pfam domain/function: PF09349 OHCU_decarbox; PF01014 Uricase [H]

EC number: =1.7.3.3 [H]

Molecular weight: Translated: 18228; Mature: 18097

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVVDDPGLDDAAFMARYRDLFEHSPWVVERALRRRPFVDVHRGLMQVVYDASPQDQVAL
CCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEE
IRAHPELAGKAAIDRTLTAASAAEQASAGLDRLDEDEFKRFHALNQAYRHRFGFPFVICV
EECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEE
RLHDKAGILAALEQRLDASRNAEIATAIAEIGKIVHLRLEALS
EECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVVDDPGLDDAAFMARYRDLFEHSPWVVERALRRRPFVDVHRGLMQVVYDASPQDQVAL
CCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEE
IRAHPELAGKAAIDRTLTAASAAEQASAGLDRLDEDEFKRFHALNQAYRHRFGFPFVICV
EECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEE
RLHDKAGILAALEQRLDASRNAEIATAIAEIGKIVHLRLEALS
EECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]