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The map label for this gene is asnB [H]

Identifier: 21240825

GI number: 21240825

Start: 61865

End: 63706

Strand: Reverse

Name: asnB [H]

Synonym: XAC0051

Alternate gene names: 21240825

Gene position: 63706-61865 (Counterclockwise)

Preceding gene: 21240826

Following gene: 21240824

Centisome position: 1.23

GC content: 66.61

Gene sequence:

>1842_bases
ATGTGCGGCATAGTCGCGCTGCGCAGCTTCGTTGCGGAGGCGCCGTTGCAGGCGCTGGCCGAGCGCGCGCTAGACGCCTT
GTCACGTCGCGGCCCGGACGCGCAGGGTCTGCTGTGCCTGGAGCAGCCAGTGCCGACGGCGCTAGGCCACCGTCGCTTGG
CGATCCTGGACTTGAGCGCTGCTGCGACCCAGCCAATGCGCTGCGCCGAGACCGGCAACGTGCTGGTGTTCAATGGCGAG
ATCTACAACTTTCTTGAGCTGCGCCGCGAACTGGAAGGGTTGGGTCATCAATTCCGCACCGACAGCGATACCGAGGTCAT
CCTGCATGGCTGGCGCGCATGGGGCGAGGGGCTGTTCCCGCGTTGCAATGGCATGTGGGCGCTGGTGCTGCTGGAGCAGG
CAAGCGGCGATCTGATCTATTGCCGCGACCGGCTCGGCGTCAAACCGTTGTACCTGCACCACGACGGGCGGCAGTTGGTG
CTGGCCAGCGAGATCCGCGCCATCGCTGCCAGCCTGGGGGGCTACCCGCCACCCAATCCCGCCGCGGTGTTCGATTTCCT
GGTTACCGGTCTCTCCGACCACACCAGCGACACCTTTTACGCCGGCATCCGCGCGGTTCCTCCCGGCTGGCTGTACCGGG
TGTCGCCAGCCGGCCATAGCCGGCGCACGCCGTACCACCAGTGGCCGCTGCCCGGTGCAGTTGCTCCGCTGGACGCGCAG
GCCACTATGGAGCTGCTCGAAGACTCCACCCGCCTGCGTTTGCGCTCGGATGCGCCCACGGTGTCACTGCTATCGGGCGG
GTTGGACAGTTCAATCCTGACCACCCTGAGCGTGCAGGCCGGTGCGCTGCCACGCACCTGTTTCGCCGGCGCCTTCACCT
ATGGCTACGACGACACCGAGCAGGCCGGCTTCGACGAAACCGAGGCCGCAGCGAGCTTGATGCAAACGCTGGGGCAGAGC
GAGCGTCACTTCGTGCATCGCGCCCGCGCGATTCCGGACGAGGAGGAACTGCTGGCACTGGTGGCGGCGCAGGAAGAGCC
GTTCTGTACGCCGTCGATACTCGCCAGTTTCCGTATGTATCGCGCCATCCGCGCCGCCGGTTACAAGGTGGTGCTCAATG
GTGAAGGCGCCGATGAGCTGTTCGGTGGCTACGTGCGCCTGTACCTGGCCTTGAGCGCGCGCAGTGCGCTGCACCAAGCA
CGACTGCCGACCGCCATCAGGCTGCTGTCCACCGGTGCGGTGGACCCGCGTCTGCTGCTCAATCGTCTTGCATGGGATCT
GCCAGCCGGCGCGCTGGGCGCACTGCTGCGCCGGCAGCGGCCGAGCGTGGCCTGCATGTCCTCGGCACTGTGGCACGACC
AAGCCGCCCGGCTGCAGTCGTTGCAGGTTGATCGCCGCGTCGATATCGAGGCGCGTTTGCGTCGCGACGTGCTGTCGACC
AATCTGCCGATGGTGTTGCGCATGACCGACCGCAACAGCATGTCCGCCGGCATCGAGGTGCGCGCGCCGTTCCTGGACTA
TCGCGTTGTCGAACGCGCGTTGTCGACGCCTGCGCTGCAGCGGATGGGCGACTATCACGGCAAGGCCATGTTGCGGCACG
CGTTCGCCGAGCGCCTGCCGGCACGCGTGACCACGCAGCGCAAGAGCACCGGCTTCGGACATGCCGAACAGTTTCTGGTC
GATCGCATGCCGTTGCAGCAGGCGCTGGCGACGTTGCCGGAAGGCGTGAACGAACTGCTGAATGTCGCGAGGCTGCGCCG
GGAGTTTGCCAGCGGACGATCGCACAGCACGCTGTGGTTCGCGGTCTCGGTCGCGCTGTGGTATCGGAGCGTCTATGCGT
AA

Upstream 100 bases:

>100_bases
TGGCAGCGCCGCAGCCTGCTGCACACCACGTTGTCAGCGCGCGCGTGATGCATGATTGCGCCTTGATTGCGATGTTCTGC
TTTCGGCATCGGGGGCCATC

Downstream 100 bases:

>100_bases
CGCGCCGCCGACGGTACAGGAGCTGTGGATGCCCGACCACGGGCATTGCCGCTGTTGCGGCGGCACGCAAGCGCAACGCA
CGGTCTGGGCGGAAGTCTAT

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 613; Mature: 613

Protein sequence:

>613_residues
MCGIVALRSFVAEAPLQALAERALDALSRRGPDAQGLLCLEQPVPTALGHRRLAILDLSAAATQPMRCAETGNVLVFNGE
IYNFLELRRELEGLGHQFRTDSDTEVILHGWRAWGEGLFPRCNGMWALVLLEQASGDLIYCRDRLGVKPLYLHHDGRQLV
LASEIRAIAASLGGYPPPNPAAVFDFLVTGLSDHTSDTFYAGIRAVPPGWLYRVSPAGHSRRTPYHQWPLPGAVAPLDAQ
ATMELLEDSTRLRLRSDAPTVSLLSGGLDSSILTTLSVQAGALPRTCFAGAFTYGYDDTEQAGFDETEAAASLMQTLGQS
ERHFVHRARAIPDEEELLALVAAQEEPFCTPSILASFRMYRAIRAAGYKVVLNGEGADELFGGYVRLYLALSARSALHQA
RLPTAIRLLSTGAVDPRLLLNRLAWDLPAGALGALLRRQRPSVACMSSALWHDQAARLQSLQVDRRVDIEARLRRDVLST
NLPMVLRMTDRNSMSAGIEVRAPFLDYRVVERALSTPALQRMGDYHGKAMLRHAFAERLPARVTTQRKSTGFGHAEQFLV
DRMPLQQALATLPEGVNELLNVARLRREFASGRSHSTLWFAVSVALWYRSVYA

Sequences:

>Translated_613_residues
MCGIVALRSFVAEAPLQALAERALDALSRRGPDAQGLLCLEQPVPTALGHRRLAILDLSAAATQPMRCAETGNVLVFNGE
IYNFLELRRELEGLGHQFRTDSDTEVILHGWRAWGEGLFPRCNGMWALVLLEQASGDLIYCRDRLGVKPLYLHHDGRQLV
LASEIRAIAASLGGYPPPNPAAVFDFLVTGLSDHTSDTFYAGIRAVPPGWLYRVSPAGHSRRTPYHQWPLPGAVAPLDAQ
ATMELLEDSTRLRLRSDAPTVSLLSGGLDSSILTTLSVQAGALPRTCFAGAFTYGYDDTEQAGFDETEAAASLMQTLGQS
ERHFVHRARAIPDEEELLALVAAQEEPFCTPSILASFRMYRAIRAAGYKVVLNGEGADELFGGYVRLYLALSARSALHQA
RLPTAIRLLSTGAVDPRLLLNRLAWDLPAGALGALLRRQRPSVACMSSALWHDQAARLQSLQVDRRVDIEARLRRDVLST
NLPMVLRMTDRNSMSAGIEVRAPFLDYRVVERALSTPALQRMGDYHGKAMLRHAFAERLPARVTTQRKSTGFGHAEQFLV
DRMPLQQALATLPEGVNELLNVARLRREFASGRSHSTLWFAVSVALWYRSVYA
>Mature_613_residues
MCGIVALRSFVAEAPLQALAERALDALSRRGPDAQGLLCLEQPVPTALGHRRLAILDLSAAATQPMRCAETGNVLVFNGE
IYNFLELRRELEGLGHQFRTDSDTEVILHGWRAWGEGLFPRCNGMWALVLLEQASGDLIYCRDRLGVKPLYLHHDGRQLV
LASEIRAIAASLGGYPPPNPAAVFDFLVTGLSDHTSDTFYAGIRAVPPGWLYRVSPAGHSRRTPYHQWPLPGAVAPLDAQ
ATMELLEDSTRLRLRSDAPTVSLLSGGLDSSILTTLSVQAGALPRTCFAGAFTYGYDDTEQAGFDETEAAASLMQTLGQS
ERHFVHRARAIPDEEELLALVAAQEEPFCTPSILASFRMYRAIRAAGYKVVLNGEGADELFGGYVRLYLALSARSALHQA
RLPTAIRLLSTGAVDPRLLLNRLAWDLPAGALGALLRRQRPSVACMSSALWHDQAARLQSLQVDRRVDIEARLRRDVLST
NLPMVLRMTDRNSMSAGIEVRAPFLDYRVVERALSTPALQRMGDYHGKAMLRHAFAERLPARVTTQRKSTGFGHAEQFLV
DRMPLQQALATLPEGVNELLNVARLRREFASGRSHSTLWFAVSVALWYRSVYA

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=415, Percent_Identity=27.9518072289157, Blast_Score=93, Evalue=1e-18,
Organism=Homo sapiens, GI168229252, Length=415, Percent_Identity=27.9518072289157, Blast_Score=93, Evalue=1e-18,
Organism=Homo sapiens, GI168229250, Length=415, Percent_Identity=27.9518072289157, Blast_Score=93, Evalue=1e-18,
Organism=Homo sapiens, GI296010848, Length=389, Percent_Identity=27.5064267352185, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI1786889, Length=452, Percent_Identity=30.9734513274336, Blast_Score=139, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI25147557, Length=406, Percent_Identity=31.0344827586207, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71993933, Length=406, Percent_Identity=31.0344827586207, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI25147560, Length=288, Percent_Identity=30.9027777777778, Blast_Score=99, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6321563, Length=428, Percent_Identity=27.3364485981308, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6325403, Length=428, Percent_Identity=27.803738317757, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI45553209, Length=409, Percent_Identity=25.4278728606357, Blast_Score=108, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 67522; Mature: 67522

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVALRSFVAEAPLQALAERALDALSRRGPDAQGLLCLEQPVPTALGHRRLAILDLSA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHCCCEEEEEEEHH
AATQPMRCAETGNVLVFNGEIYNFLELRRELEGLGHQFRTDSDTEVILHGWRAWGEGLFP
HCCCCCCHHCCCCEEEECCCHHHHHHHHHHHHHCCHHCCCCCCCCEEEEHHHHHCCCCCC
RCNGMWALVLLEQASGDLIYCRDRLGVKPLYLHHDGRQLVLASEIRAIAASLGGYPPPNP
CCCCEEEEEEEECCCCCEEEEECCCCCCEEEEECCCCEEEHHHHHHHHHHHHCCCCCCCH
AAVFDFLVTGLSDHTSDTFYAGIRAVPPGWLYRVSPAGHSRRTPYHQWPLPGAVAPLDAQ
HHHHHHHHHCCCCCCCCHHHHCCEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHH
ATMELLEDSTRLRLRSDAPTVSLLSGGLDSSILTTLSVQAGALPRTCFAGAFTYGYDDTE
HHHHHHCCCHHEEEECCCCCHHHHHCCCCHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCC
QAGFDETEAAASLMQTLGQSERHFVHRARAIPDEEELLALVAAQEEPFCTPSILASFRMY
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHH
RAIRAAGYKVVLNGEGADELFGGYVRLYLALSARSALHQARLPTAIRLLSTGAVDPRLLL
HHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHH
NRLAWDLPAGALGALLRRQRPSVACMSSALWHDQAARLQSLQVDRRVDIEARLRRDVLST
HHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHC
NLPMVLRMTDRNSMSAGIEVRAPFLDYRVVERALSTPALQRMGDYHGKAMLRHAFAERLP
CCCEEEEECCCCCCCCCCEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC
ARVTTQRKSTGFGHAEQFLVDRMPLQQALATLPEGVNELLNVARLRREFASGRSHSTLWF
CHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
AVSVALWYRSVYA
HHHHHHHHHHHCC
>Mature Secondary Structure
MCGIVALRSFVAEAPLQALAERALDALSRRGPDAQGLLCLEQPVPTALGHRRLAILDLSA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHCCCEEEEEEEHH
AATQPMRCAETGNVLVFNGEIYNFLELRRELEGLGHQFRTDSDTEVILHGWRAWGEGLFP
HCCCCCCHHCCCCEEEECCCHHHHHHHHHHHHHCCHHCCCCCCCCEEEEHHHHHCCCCCC
RCNGMWALVLLEQASGDLIYCRDRLGVKPLYLHHDGRQLVLASEIRAIAASLGGYPPPNP
CCCCEEEEEEEECCCCCEEEEECCCCCCEEEEECCCCEEEHHHHHHHHHHHHCCCCCCCH
AAVFDFLVTGLSDHTSDTFYAGIRAVPPGWLYRVSPAGHSRRTPYHQWPLPGAVAPLDAQ
HHHHHHHHHCCCCCCCCHHHHCCEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHH
ATMELLEDSTRLRLRSDAPTVSLLSGGLDSSILTTLSVQAGALPRTCFAGAFTYGYDDTE
HHHHHHCCCHHEEEECCCCCHHHHHCCCCHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCC
QAGFDETEAAASLMQTLGQSERHFVHRARAIPDEEELLALVAAQEEPFCTPSILASFRMY
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHH
RAIRAAGYKVVLNGEGADELFGGYVRLYLALSARSALHQARLPTAIRLLSTGAVDPRLLL
HHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHH
NRLAWDLPAGALGALLRRQRPSVACMSSALWHDQAARLQSLQVDRRVDIEARLRRDVLST
HHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHC
NLPMVLRMTDRNSMSAGIEVRAPFLDYRVVERALSTPALQRMGDYHGKAMLRHAFAERLP
CCCEEEEECCCCCCCCCCEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC
ARVTTQRKSTGFGHAEQFLVDRMPLQQALATLPEGVNELLNVARLRREFASGRSHSTLWF
CHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
AVSVALWYRSVYA
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]