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The map label for this gene is azoB [H]

Identifier: 21240812

GI number: 21240812

Start: 47588

End: 48457

Strand: Reverse

Name: azoB [H]

Synonym: XAC0038

Alternate gene names: 21240812

Gene position: 48457-47588 (Counterclockwise)

Preceding gene: 21240814

Following gene: 21240810

Centisome position: 0.94

GC content: 64.37

Gene sequence:

>870_bases
ATGAGCATTCTCGTCACCGGTGCCACCGGCACCGTCGGTTCCCTCGTCACCCAAGGCCTCGCCGACGCCGGCGCCCAGGT
CAAGGCGCTGGTCCGCCAGCATGGCAAGCGCCCCTTCCCGGCAGGTGTCACCGAAGTGGTGGCAGACCTCACCGACGTTG
CGTCGATGCGTGCCGCGCTGGCCTCGGTGCGCACCCTGTTCCTGTTGAATGCCGTCACTCCCGACGAAGTGACGCAGGCG
CTGATCGCCTTGAATCTGGCCAAGGAAGCCGGCGTCGAGCGCATCGTGTACCTGTCGGTGATCCATGCCGACACCTACAC
CAACGTGCCGCACTTCACCGGCAAGCACACCGTCGAGCGCATGATCGAAAGCCTGGAAATTCCGGCCACGATTCTGCGTC
CTGCGTATTTCATGCAGAACGAAGGCATGGTGCAGCAGACGATCACCGACTACGACGTTTACCCGATGCCGATCGGCGCG
CAGGGCATCGCAATGATCGATGCACGCGACATTGCCGATATTGCCGTGGTCGAGCTGCTGCGCCGCGACCGTGCCGACGG
CCCGTTGCCGCGTGTCACGCTGGAACTGGTTGGCCCACGTGCGCTGACCGGCACAGACGTTGCCAAGGTCTGGAGCGCAG
CACTTGGCCGCGAGATTGCCTACGCCGGCGATGACGTGGCGGCCTTCGAAGCACAGCTGGCGAAGTACGGTCCGCCCTGG
CTGGCCTACGACATGCGCCTGATGATGGGCGGCATCCAGCGCTTCGGCATGCATGCAGCCGAGGGCACGGTAGCCAAATT
GAAGGCCATCTTGGGCCGTCCGCTACGCACCTATGAAGACTTCGTGCGCGAGGCGACCGCGAAAGCGTGA

Upstream 100 bases:

>100_bases
GCCCTACCGCAACGAACCGACCATGCCGGCATCCGCATCACCCCACACGCCGGCGTCGGGCTGATCCGCCCATCCCTCCA
TCCACAGCAGGAGATTGACC

Downstream 100 bases:

>100_bases
TTGCATGCGGTGCAGCAAGCGCGGAGACGTTTTGGTACTTGCTGCGCAATGTGCGTTGCCTCGGGTCACTCGACGTTGCG
CTACTGCGCCGGCACCAGGT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MSILVTGATGTVGSLVTQGLADAGAQVKALVRQHGKRPFPAGVTEVVADLTDVASMRAALASVRTLFLLNAVTPDEVTQA
LIALNLAKEAGVERIVYLSVIHADTYTNVPHFTGKHTVERMIESLEIPATILRPAYFMQNEGMVQQTITDYDVYPMPIGA
QGIAMIDARDIADIAVVELLRRDRADGPLPRVTLELVGPRALTGTDVAKVWSAALGREIAYAGDDVAAFEAQLAKYGPPW
LAYDMRLMMGGIQRFGMHAAEGTVAKLKAILGRPLRTYEDFVREATAKA

Sequences:

>Translated_289_residues
MSILVTGATGTVGSLVTQGLADAGAQVKALVRQHGKRPFPAGVTEVVADLTDVASMRAALASVRTLFLLNAVTPDEVTQA
LIALNLAKEAGVERIVYLSVIHADTYTNVPHFTGKHTVERMIESLEIPATILRPAYFMQNEGMVQQTITDYDVYPMPIGA
QGIAMIDARDIADIAVVELLRRDRADGPLPRVTLELVGPRALTGTDVAKVWSAALGREIAYAGDDVAAFEAQLAKYGPPW
LAYDMRLMMGGIQRFGMHAAEGTVAKLKAILGRPLRTYEDFVREATAKA
>Mature_288_residues
SILVTGATGTVGSLVTQGLADAGAQVKALVRQHGKRPFPAGVTEVVADLTDVASMRAALASVRTLFLLNAVTPDEVTQAL
IALNLAKEAGVERIVYLSVIHADTYTNVPHFTGKHTVERMIESLEIPATILRPAYFMQNEGMVQQTITDYDVYPMPIGAQ
GIAMIDARDIADIAVVELLRRDRADGPLPRVTLELVGPRALTGTDVAKVWSAALGREIAYAGDDVAAFEAQLAKYGPPWL
AYDMRLMMGGIQRFGMHAAEGTVAKLKAILGRPLRTYEDFVREATAKA

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Uses preferentially NADPH rather than NADH as an electron donor for its activity. The enzyme reductively cleaved Orange II and carboxy-Orange II, and can a

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NmrA-type oxidoreductase family. Azoreductase type 3 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790656, Length=289, Percent_Identity=29.0657439446367, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR008030 [H]

Pfam domain/function: PF05368 NmrA [H]

EC number: NA

Molecular weight: Translated: 31087; Mature: 30956

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILVTGATGTVGSLVTQGLADAGAQVKALVRQHGKRPFPAGVTEVVADLTDVASMRAAL
CEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
ASVRTLFLLNAVTPDEVTQALIALNLAKEAGVERIVYLSVIHADTYTNVPHFTGKHTVER
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHEEEEECCCCCCCCCCCCHHHHHH
MIESLEIPATILRPAYFMQNEGMVQQTITDYDVYPMPIGAQGIAMIDARDIADIAVVELL
HHHHHCCCHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHH
RRDRADGPLPRVTLELVGPRALTGTDVAKVWSAALGREIAYAGDDVAAFEAQLAKYGPPW
HHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHCCCCH
LAYDMRLMMGGIQRFGMHAAEGTVAKLKAILGRPLRTYEDFVREATAKA
HHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SILVTGATGTVGSLVTQGLADAGAQVKALVRQHGKRPFPAGVTEVVADLTDVASMRAAL
EEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
ASVRTLFLLNAVTPDEVTQALIALNLAKEAGVERIVYLSVIHADTYTNVPHFTGKHTVER
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHEEEEECCCCCCCCCCCCHHHHHH
MIESLEIPATILRPAYFMQNEGMVQQTITDYDVYPMPIGAQGIAMIDARDIADIAVVELL
HHHHHCCCHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHH
RRDRADGPLPRVTLELVGPRALTGTDVAKVWSAALGREIAYAGDDVAAFEAQLAKYGPPW
HHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHCCCCH
LAYDMRLMMGGIQRFGMHAAEGTVAKLKAILGRPLRTYEDFVREATAKA
HHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12147495 [H]