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The map label for this gene is pdxJ

Identifier: 21240786

GI number: 21240786

Start: 13649

End: 14416

Strand: Reverse

Name: pdxJ

Synonym: XAC0012

Alternate gene names: 21240786

Gene position: 14416-13649 (Counterclockwise)

Preceding gene: 21240787

Following gene: NA

Centisome position: 0.28

GC content: 65.23

Gene sequence:

>768_bases
ATGACCACCCAGCTCAGCGTCAACGTCAACAAGATCGCAGTCCTGCGCAACTCGCGCGGCGGCGCCGACCCGGATGTGGT
GCAGGCCGCACGCGCCTGTATTGCCGCCGGCGCGCACGGCATCACCGTGCATCCGCGCCCGGACCAGCGGCATATCCGCG
CCGATGATGTGCTGGCGCTGAGCGCGCTGACACGCGAGCACGGAGTCGAATTCAATATCGAAGGCAACCCGTTTGCGCCG
CCGCGCGGCGATTATCCGGGTCTGCTGCAGTTATGTCGTGCCACGCGCCCCGAGCAGGTCACCCTGGTACCGGATGGCGA
TGGACAACTGACGTCCGATCACGGTTTCGATTTTGCGCAGGACACCGTCCAACTCGCCGAGCTGATCACAGCGTTCAAGC
GGCTGGGCAGCCGTGTCAGCCTGTTCGTCGATGCAGGTAACCCGGACATCGCCCGCGCGGCCACGCTCGGTGCGGACCGT
ATCGAGCTGTACACCGGCCCGTATGCCCACGCGCATGCCAGCGGCCAGGCCGATACCGCCTTGGCGCTGTTCGCCAACGC
CGCGCAGCGTGCCAGTGCCGCAGGCCTGGGCATCAATGCCGGGCACGATTTGTCGCAAGCCAACCTGGGCGATTTTCTCG
CCGCTGTGCCGGGTGTGCTGGAAGTCTCCATCGGCCACGCCTTGATCAGCGAAGCCCTGTATCAGGGTTTGGATGCAAGC
GTGCGCGCGTATGTCGACATTCTGCGTAGCGGCCACGTTAGCGCTTAA

Upstream 100 bases:

>100_bases
GACCCGGCCGAGCTGCGCCGCCTGCGCGAGCTGGCAGAAGCACGCAAGTCGGCTGCCCTGGCCGCCGCACGCGACAGCGA
GCCGCAGGCCTGATCGCCGC

Downstream 100 bases:

>100_bases
GTTCATGCGGTAACGTCGGCATCGATGATCGATGCCTGGGTTGGCGACAACTACGTTCGCGTAAATGGATGCTGCGGTGG
CATTGATCGACAGTCTTTCA

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MTTQLSVNVNKIAVLRNSRGGADPDVVQAARACIAAGAHGITVHPRPDQRHIRADDVLALSALTREHGVEFNIEGNPFAP
PRGDYPGLLQLCRATRPEQVTLVPDGDGQLTSDHGFDFAQDTVQLAELITAFKRLGSRVSLFVDAGNPDIARAATLGADR
IELYTGPYAHAHASGQADTALALFANAAQRASAAGLGINAGHDLSQANLGDFLAAVPGVLEVSIGHALISEALYQGLDAS
VRAYVDILRSGHVSA

Sequences:

>Translated_255_residues
MTTQLSVNVNKIAVLRNSRGGADPDVVQAARACIAAGAHGITVHPRPDQRHIRADDVLALSALTREHGVEFNIEGNPFAP
PRGDYPGLLQLCRATRPEQVTLVPDGDGQLTSDHGFDFAQDTVQLAELITAFKRLGSRVSLFVDAGNPDIARAATLGADR
IELYTGPYAHAHASGQADTALALFANAAQRASAAGLGINAGHDLSQANLGDFLAAVPGVLEVSIGHALISEALYQGLDAS
VRAYVDILRSGHVSA
>Mature_254_residues
TTQLSVNVNKIAVLRNSRGGADPDVVQAARACIAAGAHGITVHPRPDQRHIRADDVLALSALTREHGVEFNIEGNPFAPP
RGDYPGLLQLCRATRPEQVTLVPDGDGQLTSDHGFDFAQDTVQLAELITAFKRLGSRVSLFVDAGNPDIARAATLGADRI
ELYTGPYAHAHASGQADTALALFANAAQRASAAGLGINAGHDLSQANLGDFLAAVPGVLEVSIGHALISEALYQGLDASV
RAYVDILRSGHVSA

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family

Homologues:

Organism=Escherichia coli, GI1788917, Length=241, Percent_Identity=39.8340248962656, Blast_Score=134, Evalue=6e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXJ_XANAC (Q8PRF1)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_640368.1
- ProteinModelPortal:   Q8PRF1
- SMR:   Q8PRF1
- GeneID:   1154083
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC0012
- NMPDR:   fig|190486.1.peg.12
- HOGENOM:   HBG299314
- OMA:   AGHGLNY
- ProtClustDB:   PRK05265
- BioCyc:   XAXO190486:XAC0012-MONOMER
- BRENDA:   2.6.99.2
- GO:   GO:0005737
- HAMAP:   MF_00279
- InterPro:   IPR013785
- InterPro:   IPR004569
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00559

Pfam domain/function: PF03740 PdxJ; SSF63892 PyrdxlP_synth_PdxJ

EC number: =2.6.99.2

Molecular weight: Translated: 26665; Mature: 26534

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: ACT_SITE 44-44 ACT_SITE 74-74 ACT_SITE 202-202 BINDING 8-8 BINDING 19-19 BINDING 46-46 BINDING 51-51 BINDING 111-111 BINDING 203-203

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQLSVNVNKIAVLRNSRGGADPDVVQAARACIAAGAHGITVHPRPDQRHIRADDVLAL
CCCEEEECCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHH
SALTREHGVEFNIEGNPFAPPRGDYPGLLQLCRATRPEQVTLVPDGDGQLTSDHGFDFAQ
HHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHH
DTVQLAELITAFKRLGSRVSLFVDAGNPDIARAATLGADRIELYTGPYAHAHASGQADTA
HHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHCCCCEEEEEECCCHHCCCCCCCHHH
LALFANAAQRASAAGLGINAGHDLSQANLGDFLAAVPGVLEVSIGHALISEALYQGLDAS
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCCHH
VRAYVDILRSGHVSA
HHHHHHHHHCCCCCC
>Mature Secondary Structure 
TTQLSVNVNKIAVLRNSRGGADPDVVQAARACIAAGAHGITVHPRPDQRHIRADDVLAL
CCEEEECCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHH
SALTREHGVEFNIEGNPFAPPRGDYPGLLQLCRATRPEQVTLVPDGDGQLTSDHGFDFAQ
HHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHH
DTVQLAELITAFKRLGSRVSLFVDAGNPDIARAATLGADRIELYTGPYAHAHASGQADTA
HHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHCCCCEEEEEECCCHHCCCCCCCHHH
LALFANAAQRASAAGLGINAGHDLSQANLGDFLAAVPGVLEVSIGHALISEALYQGLDAS
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCCHH
VRAYVDILRSGHVSA
HHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217