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The map label for this gene is mscS [H]

Identifier: 21228739

GI number: 21228739

Start: 3145209

End: 3146321

Strand: Reverse

Name: mscS [H]

Synonym: MM_2637

Alternate gene names: 21228739

Gene position: 3146321-3145209 (Counterclockwise)

Preceding gene: 21228740

Following gene: 21228733

Centisome position: 76.81

GC content: 43.94

Gene sequence:

>1113_bases
ATGGAAACCCTTGAAACCCTGGATACATTAAATAACAGCACCCTGGATGCGATAGAAACCCCCGATTCTATTGCAGAAAA
TATATCCTATTTAGATAGAATACTGCAGAATATATTCACCTGGTTCTCGGAAAACCTGGATACGATGTTATTCGTTATTT
TTATAGGAATGGCAACCATCCTTGCCGCAAGAACCATAAACAACTTTTTGACGAGATATTATGAGAGAGCAAGCAGCCGA
CTGCATATTGATATCACAACTTTCAGGATGTTCAGACACATAGTGGTTGCAGCAGTCTATTTTATAGGACTGGTTGTTGT
AATATTCCATATCCCAAGCCTGCGCAGTCTCTCGATAGCGATGTTATCAGGTGCCGGGCTCGCTGGAATAGTAATCGGTT
TTGCAGCCCAGAACACTCTGAGCAATATTATTGCAGGGATTGCCCTTGCTCTATTCCAACCTTTCAGGGTCGGAGACAGG
CTAACTATTATGAACGAATTCGGAAAGGTTACGGACATTAACCTCAGACATACTGTAATTCTAACATGGGATAACAGGCG
CCTTATCATCCCCAATTCAGTAATCAGCAGCGAGGCAATAGTTAACTGGACAATAGAAGACCCTGCAGTTATCTGGCCAT
TAGATGTAGGGATCAGCTATGACTCGGACATCAGCCTGGCAAAGAAAATAATGGTCGAAGAAGCCAGAAAGCATCCCAAG
GTTATGACTCCCCAGATCCTGGAGTACAGCGTTGTGAAACCCGGTTTACTTAAATCCGAGACTTTTGCAAACGGGCTCAT
GAACCCGTCCGTACTCCATGCTGTAGACCCGGATTTCAGGGAAAGAGGGCAGGTTAAAGTATACGTGACAGAGTTTGGAG
ATTCTGCAATTAATCTTCGCGTGAATATGTGGTTTAAGGACAGGAGTGACGCATACGGTGCGGGGTGCGAAATCAGGGAA
GCAATCAAAAAGCGTTTTGACGATGAAGGCATTGAAATTCCCTATCCTTACAGGACCATTGTGTACAAAACAGATATTGA
AAATGAAAAGATCTCTAAAAAACCTTCCCCGGCAGAAGAAGCCAAAAAGGCCTCGGGAGAACAGGCTAACTGA

Upstream 100 bases:

>100_bases
TATCTCTGTTAATTCCCATTACAAAACTTATTTACATTCTTACAGTTTAGATATTTTGGAATCAATATTTATTTAAGTCA
TCCTGCCAAAGGTTGAGGGC

Downstream 100 bases:

>100_bases
AACCGAAGAGAAAAAACCACCAGCAGCTCAATAAAAGGCTTGATGAACTGGAATGTATTACGAGTCAATTATGAGCTGAA
ATTCAGGCAATCATACAGGG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
METLETLDTLNNSTLDAIETPDSIAENISYLDRILQNIFTWFSENLDTMLFVIFIGMATILAARTINNFLTRYYERASSR
LHIDITTFRMFRHIVVAAVYFIGLVVVIFHIPSLRSLSIAMLSGAGLAGIVIGFAAQNTLSNIIAGIALALFQPFRVGDR
LTIMNEFGKVTDINLRHTVILTWDNRRLIIPNSVISSEAIVNWTIEDPAVIWPLDVGISYDSDISLAKKIMVEEARKHPK
VMTPQILEYSVVKPGLLKSETFANGLMNPSVLHAVDPDFRERGQVKVYVTEFGDSAINLRVNMWFKDRSDAYGAGCEIRE
AIKKRFDDEGIEIPYPYRTIVYKTDIENEKISKKPSPAEEAKKASGEQAN

Sequences:

>Translated_370_residues
METLETLDTLNNSTLDAIETPDSIAENISYLDRILQNIFTWFSENLDTMLFVIFIGMATILAARTINNFLTRYYERASSR
LHIDITTFRMFRHIVVAAVYFIGLVVVIFHIPSLRSLSIAMLSGAGLAGIVIGFAAQNTLSNIIAGIALALFQPFRVGDR
LTIMNEFGKVTDINLRHTVILTWDNRRLIIPNSVISSEAIVNWTIEDPAVIWPLDVGISYDSDISLAKKIMVEEARKHPK
VMTPQILEYSVVKPGLLKSETFANGLMNPSVLHAVDPDFRERGQVKVYVTEFGDSAINLRVNMWFKDRSDAYGAGCEIRE
AIKKRFDDEGIEIPYPYRTIVYKTDIENEKISKKPSPAEEAKKASGEQAN
>Mature_370_residues
METLETLDTLNNSTLDAIETPDSIAENISYLDRILQNIFTWFSENLDTMLFVIFIGMATILAARTINNFLTRYYERASSR
LHIDITTFRMFRHIVVAAVYFIGLVVVIFHIPSLRSLSIAMLSGAGLAGIVIGFAAQNTLSNIIAGIALALFQPFRVGDR
LTIMNEFGKVTDINLRHTVILTWDNRRLIIPNSVISSEAIVNWTIEDPAVIWPLDVGISYDSDISLAKKIMVEEARKHPK
VMTPQILEYSVVKPGLLKSETFANGLMNPSVLHAVDPDFRERGQVKVYVTEFGDSAINLRVNMWFKDRSDAYGAGCEIRE
AIKKRFDDEGIEIPYPYRTIVYKTDIENEKISKKPSPAEEAKKASGEQAN

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=308, Percent_Identity=25.974025974026, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786670, Length=201, Percent_Identity=28.8557213930348, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI2367355, Length=109, Percent_Identity=32.1100917431193, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787591, Length=303, Percent_Identity=25.0825082508251, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 41614; Mature: 41614

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METLETLDTLNNSTLDAIETPDSIAENISYLDRILQNIFTWFSENLDTMLFVIFIGMATI
CCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LAARTINNFLTRYYERASSRLHIDITTFRMFRHIVVAAVYFIGLVVVIFHIPSLRSLSIA
HHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEE
MLSGAGLAGIVIGFAAQNTLSNIIAGIALALFQPFRVGDRLTIMNEFGKVTDINLRHTVI
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHCCCEEEECEEEEEE
LTWDNRRLIIPNSVISSEAIVNWTIEDPAVIWPLDVGISYDSDISLAKKIMVEEARKHPK
EEECCCEEECCCCHHCCCCEEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCC
VMTPQILEYSVVKPGLLKSETFANGLMNPSVLHAVDPDFRERGQVKVYVTEFGDSAINLR
CCCCHHHHHHHCCCCCCCHHHHHHCCCCCCEEECCCCCHHHCCCEEEEEEECCCCEEEEE
VNMWFKDRSDAYGAGCEIREAIKKRFDDEGIEIPYPYRTIVYKTDIENEKISKKPSPAEE
EEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHH
AKKASGEQAN
HHHCCCCCCC
>Mature Secondary Structure
METLETLDTLNNSTLDAIETPDSIAENISYLDRILQNIFTWFSENLDTMLFVIFIGMATI
CCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LAARTINNFLTRYYERASSRLHIDITTFRMFRHIVVAAVYFIGLVVVIFHIPSLRSLSIA
HHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEE
MLSGAGLAGIVIGFAAQNTLSNIIAGIALALFQPFRVGDRLTIMNEFGKVTDINLRHTVI
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHCCCEEEECEEEEEE
LTWDNRRLIIPNSVISSEAIVNWTIEDPAVIWPLDVGISYDSDISLAKKIMVEEARKHPK
EEECCCEEECCCCHHCCCCEEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCC
VMTPQILEYSVVKPGLLKSETFANGLMNPSVLHAVDPDFRERGQVKVYVTEFGDSAINLR
CCCCHHHHHHHCCCCCCCHHHHHHCCCCCCEEECCCCCHHHCCCEEEEEEECCCCEEEEE
VNMWFKDRSDAYGAGCEIREAIKKRFDDEGIEIPYPYRTIVYKTDIENEKISKKPSPAEE
EEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHH
AKKASGEQAN
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]