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The map label for this gene is vhoA [H]

Identifier: 21228415

GI number: 21228415

Start: 2769124

End: 2770899

Strand: Reverse

Name: vhoA [H]

Synonym: MM_2313

Alternate gene names: 21228415

Gene position: 2770899-2769124 (Counterclockwise)

Preceding gene: 21228416

Following gene: 21228414

Centisome position: 67.64

GC content: 47.8

Gene sequence:

>1776_bases
ATGGTAAACGTTACAGTTGATCCCTTAACCAGAATTGAAGGACACCAGAGAATTTCAACAGAAGTAGATGCAAACGGTGT
TATTACAGATGCACAGTCATCGTCCCTTATATTCAGGGGATTTGAACGCATACTGCAGCACCAGGACCCGAGAGACGCAG
CTTTTCTTACACAGAGGATTTGTGGAGTATGCCCGCTTTCTCATGGGTTAACAGCAACAAACGCACTTGATGAATTGTAT
GGCGTGGCTGAACATGTCCCAAAAGATGCCCTTGTTATGAGGAATATTTTCCAGGGGCTTAACATGGCTGCAAGCCATGC
AACTCATATATATGTCCTCTGGGCACCTGACCTTGCAAACCCGGCATACAAAAAGGTTCTTACACCACTCGGAGACACAG
GAAACGCAGTCTGGGATGAAATGCTTGGAAGGTTTGCACCAATAAGCTACAAGATGGATGGAGTGCCTATACCTGCAGGA
AGTTCATACCTGGCAGCAATTCCTGAAAAGAAACGCCTTCAGGAAATGATTGCACTTATCGCAGGAAGGATGCCAGGTCC
GTCAGCCCTGTATCCGGGAGGATACACATACCCGGCAACTGTTGCAGATATTACAAAACTGTCAACATACTATCTGCAGA
TTATGGACTTCATATCCGCGCACACTCTGAGAGTCCCATTCGATACATGGATCGAAAATACATACAAGGCAAGCTCCCCT
ACGAAAGCTGTAAGCTTCGTTACCGAACACCTGACTGACCTTATCAACAAGTCAACAACTTCAAATGACTTCTCAAAAGA
AGCAGGATGGGGAGATACCGAATTCTATGCAGCCTTTGGTTCGGAACTGGTAGGAGAGAAACTTCTTGGCCTTCCGGCAA
GCCTGAAACATGATACTATCGGAGGGTACCAGGACCCCTCAAAGATATGCTTCGTTGCATACGGAGGGTATTACAAGCCA
ACAGACGGATACGACCCGAGGAGCCCGGCAGGAGACAGGATTTTCACATCAGGTGTGGTATCAGGAAACCTGGAATACCT
GAAATTTGATCCGGACAAGATTACAGAGAGCACGGCACACTCATTCTACCAGAACAGTGTAAACGACCTCCCGCCAGTAA
AAGGTGAAACAGTTCCATTCACCGATCCTGAGAAAATCGTATACACAGGAGGCTCGGACAGCCAGTACAGCTGGGACAAG
GCTCCGAGATATGACGGAATTGCAGGTGAAGTCGGGCCTCTTGCACGCATGCTGAACATAAAAGAGCCGCTTGTAACAGG
ACTTGCTCTGGCACTGGCTGAAAATGGATATTCGCCAGCCAATGTTTATACAAGGATGCTGGCTCGTATACAGGAAACCG
CAATTCTCGCATATGAGCTTCTCAACTGGGTTACTGTTGACTACGAACCCGGAGGAAAGATTTCCGTGCCTCTTGACTTC
AATGCAGCTAAAGACAGTCAGGGAATGGGCCTGTGGGAAGCGCCACGTGGGGCTCTTGGCCACTGGATTTCTACAAACGG
AAGTGGCAAGGTTGCAAACTACCAGTGCATAGTTCCGGGTTCATGGTTGATGTCTCCAAGGGACAGCAATGGAATACCTG
GACCGCTTGAACAGTCTCTTATCGGCTCAAAAATTAACCCGGTTGGAGAGGTAGATTACACAAATCCAGTTGGAATATTC
CACATGGGCAGGTCATACGACCCGTGCATTTCATGCGCAGTCCACACGATAGACCTTACAGGGAAATGCGCTCCAAATAC
TCTAAGGATACTCTAA

Upstream 100 bases:

>100_bases
GCAAATATTGACACAATAGCAAAGGTAGCTGTAGGCGCAGCCGCAGTTGCTGCAGGTGTGCATGCAGTAAGGAGAATGGG
AAAAGGAGAGTGAGTGAAAA

Downstream 100 bases:

>100_bases
GTACAGTACAGGGAGATGACCCACGATGAAATCTAACAACAACCCGAAGGCGATGGTTGTTGAGCGCTATACTATAACGG
ACAGGATAGCTCATACGGTA

Product: F420-nonreducing hydrogenase I, large subunit

Products: NA

Alternate protein names: NiFe hydrogenlyase large chain [H]

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MVNVTVDPLTRIEGHQRISTEVDANGVITDAQSSSLIFRGFERILQHQDPRDAAFLTQRICGVCPLSHGLTATNALDELY
GVAEHVPKDALVMRNIFQGLNMAASHATHIYVLWAPDLANPAYKKVLTPLGDTGNAVWDEMLGRFAPISYKMDGVPIPAG
SSYLAAIPEKKRLQEMIALIAGRMPGPSALYPGGYTYPATVADITKLSTYYLQIMDFISAHTLRVPFDTWIENTYKASSP
TKAVSFVTEHLTDLINKSTTSNDFSKEAGWGDTEFYAAFGSELVGEKLLGLPASLKHDTIGGYQDPSKICFVAYGGYYKP
TDGYDPRSPAGDRIFTSGVVSGNLEYLKFDPDKITESTAHSFYQNSVNDLPPVKGETVPFTDPEKIVYTGGSDSQYSWDK
APRYDGIAGEVGPLARMLNIKEPLVTGLALALAENGYSPANVYTRMLARIQETAILAYELLNWVTVDYEPGGKISVPLDF
NAAKDSQGMGLWEAPRGALGHWISTNGSGKVANYQCIVPGSWLMSPRDSNGIPGPLEQSLIGSKINPVGEVDYTNPVGIF
HMGRSYDPCISCAVHTIDLTGKCAPNTLRIL

Sequences:

>Translated_591_residues
MVNVTVDPLTRIEGHQRISTEVDANGVITDAQSSSLIFRGFERILQHQDPRDAAFLTQRICGVCPLSHGLTATNALDELY
GVAEHVPKDALVMRNIFQGLNMAASHATHIYVLWAPDLANPAYKKVLTPLGDTGNAVWDEMLGRFAPISYKMDGVPIPAG
SSYLAAIPEKKRLQEMIALIAGRMPGPSALYPGGYTYPATVADITKLSTYYLQIMDFISAHTLRVPFDTWIENTYKASSP
TKAVSFVTEHLTDLINKSTTSNDFSKEAGWGDTEFYAAFGSELVGEKLLGLPASLKHDTIGGYQDPSKICFVAYGGYYKP
TDGYDPRSPAGDRIFTSGVVSGNLEYLKFDPDKITESTAHSFYQNSVNDLPPVKGETVPFTDPEKIVYTGGSDSQYSWDK
APRYDGIAGEVGPLARMLNIKEPLVTGLALALAENGYSPANVYTRMLARIQETAILAYELLNWVTVDYEPGGKISVPLDF
NAAKDSQGMGLWEAPRGALGHWISTNGSGKVANYQCIVPGSWLMSPRDSNGIPGPLEQSLIGSKINPVGEVDYTNPVGIF
HMGRSYDPCISCAVHTIDLTGKCAPNTLRIL
>Mature_591_residues
MVNVTVDPLTRIEGHQRISTEVDANGVITDAQSSSLIFRGFERILQHQDPRDAAFLTQRICGVCPLSHGLTATNALDELY
GVAEHVPKDALVMRNIFQGLNMAASHATHIYVLWAPDLANPAYKKVLTPLGDTGNAVWDEMLGRFAPISYKMDGVPIPAG
SSYLAAIPEKKRLQEMIALIAGRMPGPSALYPGGYTYPATVADITKLSTYYLQIMDFISAHTLRVPFDTWIENTYKASSP
TKAVSFVTEHLTDLINKSTTSNDFSKEAGWGDTEFYAAFGSELVGEKLLGLPASLKHDTIGGYQDPSKICFVAYGGYYKP
TDGYDPRSPAGDRIFTSGVVSGNLEYLKFDPDKITESTAHSFYQNSVNDLPPVKGETVPFTDPEKIVYTGGSDSQYSWDK
APRYDGIAGEVGPLARMLNIKEPLVTGLALALAENGYSPANVYTRMLARIQETAILAYELLNWVTVDYEPGGKISVPLDF
NAAKDSQGMGLWEAPRGALGHWISTNGSGKVANYQCIVPGSWLMSPRDSNGIPGPLEQSLIGSKINPVGEVDYTNPVGIF
HMGRSYDPCISCAVHTIDLTGKCAPNTLRIL

Specific function: Catalyzes the reversible oxidoreduction of molecular hydrogen, in conjunction with a specific electron acceptor, cytochrome c3 [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=632, Percent_Identity=28.3227848101266, Blast_Score=191, Evalue=9e-50,
Organism=Escherichia coli, GI1787207, Length=309, Percent_Identity=29.7734627831715, Blast_Score=105, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.2.1 [H]

Molecular weight: Translated: 64243; Mature: 64243

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNVTVDPLTRIEGHQRISTEVDANGVITDAQSSSLIFRGFERILQHQDPRDAAFLTQRI
CCEEECCHHHHHCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHH
CGVCPLSHGLTATNALDELYGVAEHVPKDALVMRNIFQGLNMAASHATHIYVLWAPDLAN
HHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCC
PAYKKVLTPLGDTGNAVWDEMLGRFAPISYKMDGVPIPAGSSYLAAIPEKKRLQEMIALI
HHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEECCCHHHHHHHHHHH
AGRMPGPSALYPGGYTYPATVADITKLSTYYLQIMDFISAHTLRVPFDTWIENTYKASSP
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEECCHHHHHCCCCCCCCC
TKAVSFVTEHLTDLINKSTTSNDFSKEAGWGDTEFYAAFGSELVGEKLLGLPASLKHDTI
HHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GGYQDPSKICFVAYGGYYKPTDGYDPRSPAGDRIFTSGVVSGNLEYLKFDPDKITESTAH
CCCCCCCCEEEEEECCEECCCCCCCCCCCCCCCEEECCEECCCEEEEEECHHHHHHHHHH
SFYQNSVNDLPPVKGETVPFTDPEKIVYTGGSDSQYSWDKAPRYDGIAGEVGPLARMLNI
HHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
KEPLVTGLALALAENGYSPANVYTRMLARIQETAILAYELLNWVTVDYEPGGKISVPLDF
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEECC
NAAKDSQGMGLWEAPRGALGHWISTNGSGKVANYQCIVPGSWLMSPRDSNGIPGPLEQSL
CCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEEEEEEECCCEECCCCCCCCCCCHHHHHH
IGSKINPVGEVDYTNPVGIFHMGRSYDPCISCAVHTIDLTGKCAPNTLRIL
HCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHEEEEEEECCCCCCCCEECC
>Mature Secondary Structure
MVNVTVDPLTRIEGHQRISTEVDANGVITDAQSSSLIFRGFERILQHQDPRDAAFLTQRI
CCEEECCHHHHHCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHH
CGVCPLSHGLTATNALDELYGVAEHVPKDALVMRNIFQGLNMAASHATHIYVLWAPDLAN
HHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCC
PAYKKVLTPLGDTGNAVWDEMLGRFAPISYKMDGVPIPAGSSYLAAIPEKKRLQEMIALI
HHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEECCCHHHHHHHHHHH
AGRMPGPSALYPGGYTYPATVADITKLSTYYLQIMDFISAHTLRVPFDTWIENTYKASSP
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEECCHHHHHCCCCCCCCC
TKAVSFVTEHLTDLINKSTTSNDFSKEAGWGDTEFYAAFGSELVGEKLLGLPASLKHDTI
HHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GGYQDPSKICFVAYGGYYKPTDGYDPRSPAGDRIFTSGVVSGNLEYLKFDPDKITESTAH
CCCCCCCCEEEEEECCEECCCCCCCCCCCCCCCEEECCEECCCEEEEEECHHHHHHHHHH
SFYQNSVNDLPPVKGETVPFTDPEKIVYTGGSDSQYSWDKAPRYDGIAGEVGPLARMLNI
HHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
KEPLVTGLALALAENGYSPANVYTRMLARIQETAILAYELLNWVTVDYEPGGKISVPLDF
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEECC
NAAKDSQGMGLWEAPRGALGHWISTNGSGKVANYQCIVPGSWLMSPRDSNGIPGPLEQSL
CCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEEEEEEECCCEECCCCCCCCCCCHHHHHH
IGSKINPVGEVDYTNPVGIFHMGRSYDPCISCAVHTIDLTGKCAPNTLRIL
HCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHEEEEEEECCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2269874; 9438867 [H]