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The map label for this gene is wrbA

Identifier: 21228326

GI number: 21228326

Start: 2650713

End: 2651342

Strand: Direct

Name: wrbA

Synonym: MM_2224

Alternate gene names: 21228326

Gene position: 2650713-2651342 (Clockwise)

Preceding gene: 21228319

Following gene: 21228327

Centisome position: 64.71

GC content: 49.52

Gene sequence:

>630_bases
ATGGTTAAGGTAAATATAATATTTCACAGTGTTCACGCGCACATTTACAGGATGGCAGAAGCTGTCGCTGCAGGCGCAAG
GGAAGTTGAGGGGGCAGAGGTTGGGATTTATCAGGTCCCGGAAACCCTGCCGGAGGACGTTCTTGAGAAAATGGGAGCAA
TAGAAACCAAAAAACTTTTTGCCCACATTCCGGTCGTGACGCGGGATATGTATGAAGATGTCCTTGCTGGAGCCGATGCA
TTGATATTCGGTACTCCCACACGTTACGGGATGATGACTGCCCAGATGCGTGCGGTCCTTGACGGGCTTGGAAAACTGTG
GAGTGAGGACGCTTTTGTAGGAAAGGTGGGGAGCGTATTTACTTCCAGCGGAACGCAGCATGGCGGACAGGAATCTACTA
TACTCTCATTTCATGTTACCCTGCTTCACCTTGGAATGGTTATTGTCGGTCTTCCCTATGCAGAAAAGCGGCAGACAATA
ATGAACGAAATTACAGGCGGCAGCCCATATGGGGCTTCAACGATTGCAGGTGGGGACGGGAGCCGCCAGCCTTCAGAGAA
TGAACTTGAGATGGCACGTTACCAGGGCAGGCATGTAACTCAGATAGCAAAAAAAATTGCTGGAAAATAA

Upstream 100 bases:

>100_bases
AGGGGTTCTGGAGTTTTCAGGAGCTTAAATCAGGCTATCTTATTTTTGGGACCTCAATCACATCTCAGTATAATTTTAAC
GGCTTTTTGAAGGTGATTGG

Downstream 100 bases:

>100_bases
TATCTCTAAAAATAAAAAGGGCGGAAAAACATTCCCTTCTCCCGGGTGTTTTCCCTTTCAGTTTTTGAGTTATTTTTGCC
TGAAATAAGAATGCTTAGCT

Product: TrpR binding protein WrbA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MVKVNIIFHSVHAHIYRMAEAVAAGAREVEGAEVGIYQVPETLPEDVLEKMGAIETKKLFAHIPVVTRDMYEDVLAGADA
LIFGTPTRYGMMTAQMRAVLDGLGKLWSEDAFVGKVGSVFTSSGTQHGGQESTILSFHVTLLHLGMVIVGLPYAEKRQTI
MNEITGGSPYGASTIAGGDGSRQPSENELEMARYQGRHVTQIAKKIAGK

Sequences:

>Translated_209_residues
MVKVNIIFHSVHAHIYRMAEAVAAGAREVEGAEVGIYQVPETLPEDVLEKMGAIETKKLFAHIPVVTRDMYEDVLAGADA
LIFGTPTRYGMMTAQMRAVLDGLGKLWSEDAFVGKVGSVFTSSGTQHGGQESTILSFHVTLLHLGMVIVGLPYAEKRQTI
MNEITGGSPYGASTIAGGDGSRQPSENELEMARYQGRHVTQIAKKIAGK
>Mature_209_residues
MVKVNIIFHSVHAHIYRMAEAVAAGAREVEGAEVGIYQVPETLPEDVLEKMGAIETKKLFAHIPVVTRDMYEDVLAGADA
LIFGTPTRYGMMTAQMRAVLDGLGKLWSEDAFVGKVGSVFTSSGTQHGGQESTILSFHVTLLHLGMVIVGLPYAEKRQTI
MNEITGGSPYGASTIAGGDGSRQPSENELEMARYQGRHVTQIAKKIAGK

Specific function: Seems To Enhance The Formation And/Or Stability Of Noncovalent Complexes Between The Trp Repressor Protein And Operator-Bearing DNA. However, Wrba Does Not Specifically Influence The Affinity Or Mode Of Binding Of Trpr To Its Operator. It Seems Possible T

COG id: COG0655

COG function: function code R; Multimeric flavodoxin WrbA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain

Homologues:

Organism=Escherichia coli, GI1787239, Length=208, Percent_Identity=50.9615384615385, Blast_Score=191, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6319849, Length=208, Percent_Identity=40.8653846153846, Blast_Score=143, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6320235, Length=208, Percent_Identity=39.9038461538462, Blast_Score=134, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6319526, Length=207, Percent_Identity=31.8840579710145, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): WRBA_METMA (Q8PUV4)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_634248.1
- ProteinModelPortal:   Q8PUV4
- GeneID:   1480566
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_2224
- NMPDR:   fig|192952.1.peg.2224
- HOGENOM:   HBG686708
- OMA:   PYSHQGQ
- ProtClustDB:   PRK03767
- BioCyc:   MMAZ192952:MM2224-MONOMER
- HAMAP:   MF_01017
- InterPro:   IPR008254
- InterPro:   IPR010089
- InterPro:   IPR005025
- TIGRFAMs:   TIGR01755

Pfam domain/function: PF03358 FMN_red

EC number: NA

Molecular weight: Translated: 22532; Mature: 22532

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKVNIIFHSVHAHIYRMAEAVAAGAREVEGAEVGIYQVPETLPEDVLEKMGAIETKKLF
CEEEEEEHHHHHHHHHHHHHHHHHCHHHCCCCEECEECCCCCCHHHHHHHHCCHHHHHHH
AHIPVVTRDMYEDVLAGADALIFGTPTRYGMMTAQMRAVLDGLGKLWSEDAFVGKVGSVF
HHCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
TSSGTQHGGQESTILSFHVTLLHLGMVIVGLPYAEKRQTIMNEITGGSPYGASTIAGGDG
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEECCCC
SRQPSENELEMARYQGRHVTQIAKKIAGK
CCCCCHHHHHHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure
MVKVNIIFHSVHAHIYRMAEAVAAGAREVEGAEVGIYQVPETLPEDVLEKMGAIETKKLF
CEEEEEEHHHHHHHHHHHHHHHHHCHHHCCCCEECEECCCCCCHHHHHHHHCCHHHHHHH
AHIPVVTRDMYEDVLAGADALIFGTPTRYGMMTAQMRAVLDGLGKLWSEDAFVGKVGSVF
HHCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
TSSGTQHGGQESTILSFHVTLLHLGMVIVGLPYAEKRQTIMNEITGGSPYGASTIAGGDG
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEECCCC
SRQPSENELEMARYQGRHVTQIAKKIAGK
CCCCCHHHHHHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824