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The map label for this gene is mtbC [H]

Identifier: 21228154

GI number: 21228154

Start: 2447692

End: 2448360

Strand: Reverse

Name: mtbC [H]

Synonym: MM_2052

Alternate gene names: 21228154

Gene position: 2448360-2447692 (Counterclockwise)

Preceding gene: 21228155

Following gene: 21228153

Centisome position: 59.77

GC content: 48.58

Gene sequence:

>669_bases
TTGAAATGGGGGTTGAGAATGACAAGCAAAGAGGAGTTACTTCAGGAGCTTTCGGAAGCAATAATCTCCTGCAAAAAGGA
TGCAGTGCTCGCTGCAGTTGAAAAAGCAAAGCAGGTTATGGAGCCTGCGGAAATAATCGAAAATGGGCTTGCTGCCGGCA
TGAACCAGGTGGGAGTTTTATTTGAGAGAGGAAAACTCTTCCTGCCGCATGTGATGATGGCAGCCGATGCAATGACTGCC
GGGGTCAAAGTCCTTGAGGCGGATATGCCTGCAGGGACAGAGACAAAGAAACTTGGAGTCATTGTAAATGGGACAGTTGA
AGGAGACGTTCATGATATAGGGAAATCAATCGTCTCAACAATGCTGCAGTCTGCAGGCTTTGAAGTCCACGACATAGGCA
GGGACGTTCCCATAAAGAACTTTGTCGAGAAAGCAAAAGAAGTTAATGCAAACATGATAGGGATCTCCGCGCTTATGACC
ACAACTCTGCAGGGCCAGAGAGAGGTTATTGAACTTCTTAAAGAAGAAGGCATGAGGGAGAAGGTGAAAGTGATGGTTGG
CGGTGCCCCTGCAACCCAGGCGTGGGCTGACAAGATAGGTGCGGACTGCTACGCTGAGAATGCAAGCGAAGCTGTAGCAA
AGGCAAAAGAACTGCTTGTGGGGAAGTGA

Upstream 100 bases:

>100_bases
ATATCCCGGATATCGGTTTTTCTGAAAAAGTGTTTTATTTTTATACAGTGAGTAAGGAATTTTCTGGGCGTGAAGCATTT
ATCCTGTTTTTAAATATGAC

Downstream 100 bases:

>100_bases
AAAGAAATGGCAACTGAATATGCTTTGAGAATGGGAGACGGAAAGAGGGTTTTCCTTGCCAGGGAAAAAATTATGGAGGA
GATTGAGGCAGGTACTGCAA

Product: dimethylamine corrinoid protein

Products: L-Methionine; 5,6,7,8-Tetrahydrofolate [C]

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MKWGLRMTSKEELLQELSEAIISCKKDAVLAAVEKAKQVMEPAEIIENGLAAGMNQVGVLFERGKLFLPHVMMAADAMTA
GVKVLEADMPAGTETKKLGVIVNGTVEGDVHDIGKSIVSTMLQSAGFEVHDIGRDVPIKNFVEKAKEVNANMIGISALMT
TTLQGQREVIELLKEEGMREKVKVMVGGAPATQAWADKIGADCYAENASEAVAKAKELLVGK

Sequences:

>Translated_222_residues
MKWGLRMTSKEELLQELSEAIISCKKDAVLAAVEKAKQVMEPAEIIENGLAAGMNQVGVLFERGKLFLPHVMMAADAMTA
GVKVLEADMPAGTETKKLGVIVNGTVEGDVHDIGKSIVSTMLQSAGFEVHDIGRDVPIKNFVEKAKEVNANMIGISALMT
TTLQGQREVIELLKEEGMREKVKVMVGGAPATQAWADKIGADCYAENASEAVAKAKELLVGK
>Mature_222_residues
MKWGLRMTSKEELLQELSEAIISCKKDAVLAAVEKAKQVMEPAEIIENGLAAGMNQVGVLFERGKLFLPHVMMAADAMTA
GVKVLEADMPAGTETKKLGVIVNGTVEGDVHDIGKSIVSTMLQSAGFEVHDIGRDVPIKNFVEKAKEVNANMIGISALMT
TTLQGQREVIELLKEEGMREKVKVMVGGAPATQAWADKIGADCYAENASEAVAKAKELLVGK

Specific function: Acts as a methyl group carrier between mtbB and mtbA [H]

COG id: COG5012

COG function: function code R; Predicted cobalamin binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=170, Percent_Identity=37.0588235294118, Blast_Score=86, Evalue=2e-17,
Organism=Escherichia coli, GI1790450, Length=193, Percent_Identity=38.860103626943, Blast_Score=118, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17535405, Length=195, Percent_Identity=34.3589743589744, Blast_Score=93, Evalue=1e-19,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR003759
- InterPro:   IPR012741 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2 [H]

EC number: 2.1.1.13 [C]

Molecular weight: Translated: 23752; Mature: 23752

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.9 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKWGLRMTSKEELLQELSEAIISCKKDAVLAAVEKAKQVMEPAEIIENGLAAGMNQVGVL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCHHHHHHH
FERGKLFLPHVMMAADAMTAGVKVLEADMPAGTETKKLGVIVNGTVEGDVHDIGKSIVST
HHCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHH
MLQSAGFEVHDIGRDVPIKNFVEKAKEVNANMIGISALMTTTLQGQREVIELLKEEGMRE
HHHHCCCCHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCHHH
KVKVMVGGAPATQAWADKIGADCYAENASEAVAKAKELLVGK
HHHEEECCCCCHHHHHHHHCCHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKWGLRMTSKEELLQELSEAIISCKKDAVLAAVEKAKQVMEPAEIIENGLAAGMNQVGVL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCHHHHHHH
FERGKLFLPHVMMAADAMTAGVKVLEADMPAGTETKKLGVIVNGTVEGDVHDIGKSIVST
HHCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHH
MLQSAGFEVHDIGRDVPIKNFVEKAKEVNANMIGISALMTTTLQGQREVIELLKEEGMRE
HHHHCCCCHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCHHH
KVKVMVGGAPATQAWADKIGADCYAENASEAVAKAKELLVGK
HHHEEECCCCCHHHHHHHHCCHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP; Cobinamide; S-adenosyl-L-methionine [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 500-780 [C]

Specific activity: 3.8

Km value (mM): 0.035 {S-methyltetrahydrofolate}} 0.0016 {S-adenosyl-L-methionine}} 0.00065 {S-adenosyl-L-methionine}} 0.06 {methyltetrahydrofolate}} 0.03 {methyltetrahydrofolate}} 0.016 {homocysteine}} [C]

Substrates: 5-Methyltetrahydrofolate; L-Homocysteine [C]

Specific reaction: 5-Methyltetrahydrofolate + L-Homocysteine --> L-Methionine + 5,6,7,8-Tetrahydrofolate [C]

General reaction: Methyl group transfer [C]

Inhibitor: 5, 6-Dimethyl benzimidazole; alpha-Ribazole 3'-phosphate; Be2+; Cobinamide; Propyl iodide; Sr2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824 [H]