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The map label for this gene is rio1 [H]

Identifier: 21228115

GI number: 21228115

Start: 2398726

End: 2399514

Strand: Reverse

Name: rio1 [H]

Synonym: MM_2013

Alternate gene names: 21228115

Gene position: 2399514-2398726 (Counterclockwise)

Preceding gene: 21228116

Following gene: 21228114

Centisome position: 58.58

GC content: 43.6

Gene sequence:

>789_bases
ATGAGCAGGGATCTGGAAGAGAAAGTAAAGCGCATTGACAGTGCTACAGACAAAGCCCGGGCAAGAGAAAAAGACTCTGA
TCGGCTGAAGGTGGAAGAGAATGTATTTGATGTTCCCACCCTTAAAATCCTTTATACACTTTCAAATAAAGGGATAATAA
AAGCAATGGGAGGAGCCATCAGTACTGGGAAGGAAGCAAATGTTTTCTATGCAGAAGGCGATGAAAAAGAACTGGCAATA
AAGATATACAGGATGGCCAGCAGCACTTTCAAGGCAATGGATGCCTATATTATGAAAGACCCCCGCTTCACGAACATTCG
AAACAACAGGCGAGATATCATTTTTGCATGGACGCGCAAGGAACTGCAGAACCTGAAGCGTGCAAAGAGCGCAGGCGTAA
GGGTGCCTGAACCAATAGTTGCTGAAAAGAATGTCCTTATTATGGAATTCATGGGAGAAGAAAAGAAACCCTATCCCCTG
TTGAAGAACACTCCTCTGGAAGATGATGAGGCAAAGCTTGTTTATAACAAAATCGTTGAATACATGCATCTCCTTTATAA
AGAAGCAAACCTTGTGCATGCTGACCTGAGTGAATATAACATTCTCATTGACCCGGCAGATAAGACCCCGGTCTTTATTG
ATATGGGGCAGTCCGTAACCCTGGAACATCCCAATGCAAGAGAGTTTCTCTACAGAGACGTGCTAAATATACTCAGGTTC
TTTGGCCGCTATGGGATCACGGACAAACCTGAAGAACTGCTTTCAAAAATACAGGCGGAAACAACATGA

Upstream 100 bases:

>100_bases
TGCCCTGGGAATTCCAGAATGAAAAAGCAGATGTGATCTGGAAATACACAAGGCCGCAGGTAGACTGGCTTGAAAGAAAA
GGATACCTTAAAGGATAAAC

Downstream 100 bases:

>100_bases
CTCAGTATGTCAAAATCCCCAGAGAAAGAATCGGAGTAATCATCGGTCCTAAAGGAGAAATAAAAAAACTCATAGAGGAC
AAAACCACATGCCAGCTGGA

Product: Serine/threonine protein kinase

Products: NA

Alternate protein names: AfRio1 [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MSRDLEEKVKRIDSATDKARAREKDSDRLKVEENVFDVPTLKILYTLSNKGIIKAMGGAISTGKEANVFYAEGDEKELAI
KIYRMASSTFKAMDAYIMKDPRFTNIRNNRRDIIFAWTRKELQNLKRAKSAGVRVPEPIVAEKNVLIMEFMGEEKKPYPL
LKNTPLEDDEAKLVYNKIVEYMHLLYKEANLVHADLSEYNILIDPADKTPVFIDMGQSVTLEHPNAREFLYRDVLNILRF
FGRYGITDKPEELLSKIQAETT

Sequences:

>Translated_262_residues
MSRDLEEKVKRIDSATDKARAREKDSDRLKVEENVFDVPTLKILYTLSNKGIIKAMGGAISTGKEANVFYAEGDEKELAI
KIYRMASSTFKAMDAYIMKDPRFTNIRNNRRDIIFAWTRKELQNLKRAKSAGVRVPEPIVAEKNVLIMEFMGEEKKPYPL
LKNTPLEDDEAKLVYNKIVEYMHLLYKEANLVHADLSEYNILIDPADKTPVFIDMGQSVTLEHPNAREFLYRDVLNILRF
FGRYGITDKPEELLSKIQAETT
>Mature_261_residues
SRDLEEKVKRIDSATDKARAREKDSDRLKVEENVFDVPTLKILYTLSNKGIIKAMGGAISTGKEANVFYAEGDEKELAIK
IYRMASSTFKAMDAYIMKDPRFTNIRNNRRDIIFAWTRKELQNLKRAKSAGVRVPEPIVAEKNVLIMEFMGEEKKPYPLL
KNTPLEDDEAKLVYNKIVEYMHLLYKEANLVHADLSEYNILIDPADKTPVFIDMGQSVTLEHPNAREFLYRDVLNILRFF
GRYGITDKPEELLSKIQAETT

Specific function: Autophosphorylation of the rio1 protein is not necessary for maintenance of kinase activity. Prefers ATP over GTP. The yeast ortholog is involved in ribosome biogenesis [H]

COG id: COG1718

COG function: function code TD; Serine/threonine protein kinase involved in cell cycle control

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 protein kinase domain [H]

Homologues:

Organism=Homo sapiens, GI23510356, Length=245, Percent_Identity=39.5918367346939, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI22325377, Length=246, Percent_Identity=37.8048780487805, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI23510358, Length=134, Percent_Identity=41.044776119403, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI229331967, Length=206, Percent_Identity=28.6407766990291, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI229331971, Length=206, Percent_Identity=28.6407766990291, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI115532073, Length=240, Percent_Identity=40.4166666666667, Blast_Score=178, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17556977, Length=237, Percent_Identity=37.5527426160338, Blast_Score=160, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI71989043, Length=166, Percent_Identity=38.5542168674699, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI32564178, Length=209, Percent_Identity=26.7942583732057, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324693, Length=275, Percent_Identity=36, Blast_Score=150, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6324122, Length=237, Percent_Identity=25.7383966244726, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24662811, Length=234, Percent_Identity=41.025641025641, Blast_Score=173, Evalue=1e-43,
Organism=Drosophila melanogaster, GI21356879, Length=234, Percent_Identity=41.025641025641, Blast_Score=173, Evalue=1e-43,
Organism=Drosophila melanogaster, GI19920700, Length=251, Percent_Identity=39.4422310756972, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24649986, Length=232, Percent_Identity=25.8620689655172, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011009
- InterPro:   IPR000719
- InterPro:   IPR018934
- InterPro:   IPR000687
- InterPro:   IPR018935 [H]

Pfam domain/function: PF01163 RIO1 [H]

EC number: =2.7.11.1 [H]

Molecular weight: Translated: 30202; Mature: 30071

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: PS00109 PROTEIN_KINASE_TYR ; PS50011 PROTEIN_KINASE_DOM ; PS01245 RIO1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRDLEEKVKRIDSATDKARAREKDSDRLKVEENVFDVPTLKILYTLSNKGIIKAMGGAI
CCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCEEEEEEECCCCEEEECCCCC
STGKEANVFYAEGDEKELAIKIYRMASSTFKAMDAYIMKDPRFTNIRNNRRDIIFAWTRK
CCCCCCEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECHH
ELQNLKRAKSAGVRVPEPIVAEKNVLIMEFMGEEKKPYPLLKNTPLEDDEAKLVYNKIVE
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
YMHLLYKEANLVHADLSEYNILIDPADKTPVFIDMGQSVTLEHPNAREFLYRDVLNILRF
HHHHHHHHCCEEEECCCCCEEEECCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHH
FGRYGITDKPEELLSKIQAETT
HHCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
SRDLEEKVKRIDSATDKARAREKDSDRLKVEENVFDVPTLKILYTLSNKGIIKAMGGAI
CCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCEEEEEEECCCCEEEECCCCC
STGKEANVFYAEGDEKELAIKIYRMASSTFKAMDAYIMKDPRFTNIRNNRRDIIFAWTRK
CCCCCCEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECHH
ELQNLKRAKSAGVRVPEPIVAEKNVLIMEFMGEEKKPYPLLKNTPLEDDEAKLVYNKIVE
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
YMHLLYKEANLVHADLSEYNILIDPADKTPVFIDMGQSVTLEHPNAREFLYRDVLNILRF
HHHHHHHHCCEEEECCCCCEEEECCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHH
FGRYGITDKPEELLSKIQAETT
HHCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]