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The map label for this gene is eif2a [H]

Identifier: 21227909

GI number: 21227909

Start: 2157380

End: 2158195

Strand: Reverse

Name: eif2a [H]

Synonym: MM_1807

Alternate gene names: 21227909

Gene position: 2158195-2157380 (Counterclockwise)

Preceding gene: 21227910

Following gene: 298205309

Centisome position: 52.69

GC content: 47.92

Gene sequence:

>816_bases
GTGGTACGAATGGGAAACGATAACTGGCCTGAAGTCGGAGAGTTTGTTGTCTGTACGGTGAAGAATGTAACTGATTTCGG
AGCCTACGTCGAGCTTGAGGAATTCGGAGGAAGGGAGGGTTTCATCCATATCTCCGAAATCAAAGCAGGCTGGGTCAAAT
ACGTCAGGGACTACGTCAGGGAAGGGCAGAAGATTGTCTGCAAGGTTCTGAACGTGGACCCTTCCCGCGGCCACATAGAC
CTTTCATTGAAAGACGTGAACGAGCACCAGCGGCGGGCTAAAATCCAGGAATGGAAGAATGAGCAGAAAGCCGCCAAGTG
GCTCCAGTTCGTGGCCGAAGAAACCAAGACAGATGAAAACGGCCTGCAGGCTCTTCACGAGAGACTTGTTGAAGATTTCG
GAAGCGCATATTCAGCCTTTGAAGAAGCAGCTATCGAAGGGGAGAGGGCTTTTAAAGGGCTCAAAGTCAACAAGAAATAC
CTGAAGAGCATAGTCAAAATCGCAGGAGAAAATATCAAACTGCCCTTCGTGGATATCGCAGGGTATGTTGATCTGACCTG
TAATCTTCCAAACGGCATAGAGGTCATAAAACAATCCCTCAATGCTGCCAATTCCATCAGCGATGTGGATGGAAAAGATA
TCAGGCTCGAAATAACTTACACAGGAGCCCCAAGATACAGAATCAAGGTAATAGCTCCTGACTATAAAAAAGCCGAATCA
GTCCTTAAGAAATCCGCCCAGACGGCAGTAGACACTATTTCCAAACTTGGCGGACATGGGATCTTCAAACGGCATATCGA
ATCGGCAAAGGCGTGA

Upstream 100 bases:

>100_bases
TACAATTACCACTCACATCCTGGAAGTGCTTGAATAACCGGACAGGTAAAAGCACTGCACAGGAGTACAGTTGAGAGTAA
TAAAAGACAAAAATTGAGAA

Downstream 100 bases:

>100_bases
TACCCTTTGGGACAAAAGATCCGCAAATGCAAAAATTGCGGCAGGTACACTTTAAGGGAAAAATGTCCGGTTTGCGGGGA
AGTAACTTTACCCGCTATTC

Product: translation initiation factor IF-2 subunit alpha

Products: RNAn; a nucleoside diphosphate [C]

Alternate protein names: aIF2-alpha; eIF-2-alpha [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MVRMGNDNWPEVGEFVVCTVKNVTDFGAYVELEEFGGREGFIHISEIKAGWVKYVRDYVREGQKIVCKVLNVDPSRGHID
LSLKDVNEHQRRAKIQEWKNEQKAAKWLQFVAEETKTDENGLQALHERLVEDFGSAYSAFEEAAIEGERAFKGLKVNKKY
LKSIVKIAGENIKLPFVDIAGYVDLTCNLPNGIEVIKQSLNAANSISDVDGKDIRLEITYTGAPRYRIKVIAPDYKKAES
VLKKSAQTAVDTISKLGGHGIFKRHIESAKA

Sequences:

>Translated_271_residues
MVRMGNDNWPEVGEFVVCTVKNVTDFGAYVELEEFGGREGFIHISEIKAGWVKYVRDYVREGQKIVCKVLNVDPSRGHID
LSLKDVNEHQRRAKIQEWKNEQKAAKWLQFVAEETKTDENGLQALHERLVEDFGSAYSAFEEAAIEGERAFKGLKVNKKY
LKSIVKIAGENIKLPFVDIAGYVDLTCNLPNGIEVIKQSLNAANSISDVDGKDIRLEITYTGAPRYRIKVIAPDYKKAES
VLKKSAQTAVDTISKLGGHGIFKRHIESAKA
>Mature_271_residues
MVRMGNDNWPEVGEFVVCTVKNVTDFGAYVELEEFGGREGFIHISEIKAGWVKYVRDYVREGQKIVCKVLNVDPSRGHID
LSLKDVNEHQRRAKIQEWKNEQKAAKWLQFVAEETKTDENGLQALHERLVEDFGSAYSAFEEAAIEGERAFKGLKVNKKY
LKSIVKIAGENIKLPFVDIAGYVDLTCNLPNGIEVIKQSLNAANSISDVDGKDIRLEITYTGAPRYRIKVIAPDYKKAES
VLKKSAQTAVDTISKLGGHGIFKRHIESAKA

Specific function: eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA [H]

COG id: COG1093

COG function: function code J; Translation initiation factor 2, alpha subunit (eIF-2alpha)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI4758256, Length=274, Percent_Identity=23.3576642335766, Blast_Score=75, Evalue=7e-14,
Organism=Escherichia coli, GI145693187, Length=81, Percent_Identity=41.9753086419753, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17509871, Length=236, Percent_Identity=27.1186440677966, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322466, Length=258, Percent_Identity=25.968992248062, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011488
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR022964 [H]

Pfam domain/function: PF07541 EIF_2_alpha; PF00575 S1 [H]

EC number: 2.7.7.8 [C]

Molecular weight: Translated: 30416; Mature: 30416

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRMGNDNWPEVGEFVVCTVKNVTDFGAYVELEEFGGREGFIHISEIKAGWVKYVRDYVR
CCCCCCCCCCCCCCEEEEEECCCCCCCCEEEHHHCCCCCCEEEHHHHHHHHHHHHHHHHH
EGQKIVCKVLNVDPSRGHIDLSLKDVNEHQRRAKIQEWKNEQKAAKWLQFVAEETKTDEN
CCHHEEHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
GLQALHERLVEDFGSAYSAFEEAAIEGERAFKGLKVNKKYLKSIVKIAGENIKLPFVDIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEEEC
GYVDLTCNLPNGIEVIKQSLNAANSISDVDGKDIRLEITYTGAPRYRIKVIAPDYKKAES
CEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCEEEEEECCCHHHHHH
VLKKSAQTAVDTISKLGGHGIFKRHIESAKA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure
MVRMGNDNWPEVGEFVVCTVKNVTDFGAYVELEEFGGREGFIHISEIKAGWVKYVRDYVR
CCCCCCCCCCCCCCEEEEEECCCCCCCCEEEHHHCCCCCCEEEHHHHHHHHHHHHHHHHH
EGQKIVCKVLNVDPSRGHIDLSLKDVNEHQRRAKIQEWKNEQKAAKWLQFVAEETKTDEN
CCHHEEHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
GLQALHERLVEDFGSAYSAFEEAAIEGERAFKGLKVNKKYLKSIVKIAGENIKLPFVDIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEEEC
GYVDLTCNLPNGIEVIKQSLNAANSISDVDGKDIRLEITYTGAPRYRIKVIAPDYKKAES
CEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCEEEEEECCCHHHHHH
VLKKSAQTAVDTISKLGGHGIFKRHIESAKA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 4500 [C]

Specific activity: NA

Km value (mM): NA

Substrates: RNAn+1; phosphate [C]

Specific reaction: RNAn+1 + phosphate = RNAn + a nucleoside diphosphate [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA