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The map label for this gene is rfk [H]

Identifier: 21227811

GI number: 21227811

Start: 2051522

End: 2052199

Strand: Direct

Name: rfk [H]

Synonym: MM_1709

Alternate gene names: 21227811

Gene position: 2051522-2052199 (Clockwise)

Preceding gene: 21227803

Following gene: 21227812

Centisome position: 50.08

GC content: 45.43

Gene sequence:

>678_bases
GTGCCGGACATTGAATACCTGAAGAAACTTGCCCTTATAGGGGCAGTAAATAAAACTGTTAAAGTCTCTTCGAGTGAGTT
TCACAAGCATACGGGAGACAGTTCCAAAACCGCAGCTCGAAAGTTAAAACAGCTTGAGAAAGAGCGGTTGATCGAAAGAG
AACTGGTGCCCGGTGGCCAGTTGATAAAAATGACAGAAAAAGGGATTGAAATCCTGAAGAACGAATACGTCGAATACAGC
AGGATCTTTTCTTCAGAGCCCGATACTCTGGAGCTTGAAGGAAACGTCCTCAAAGGTCTTGGTGAAGGTCAGTATTATAT
AAATATCCCGGGGTACAGGACTCAATTTGAAGAAAAACTGCACTTCATCCCCTTCCCCGGAACCCTTAACGTGCAGCTCT
CGGAAAACAGTTCAGCTCTCAGGAACAGGCTTCGAGAAATGCCTTCTGTCCGGATAGACGGTTTTAATGATGGAGAACGC
ACTTTCGGGGGTGGAAATTGTTATCCTGTCAGAGTAGAGGGCATAGATGCTGCTGTTGTAGTCCCTGACAGGACTCATTA
CCCTTCGGATTTAATTGAGATTATTGCACCGGTAAAACTCAGGGATGCCCTGAAATTGAAAGATGGAGACAGAGTTGTGA
CACAGCTGAAAAAGCAGGGTATGGAGGGACAGAAATGA

Upstream 100 bases:

>100_bases
TGCCTCGCTCGATATCAAAAGTTTTATTATATTGAAATGCGTAGTTTTCTATCTCATAATTGAGACGCATCTCAAAATTA
AGGTAACATGGGTGCCGACA

Downstream 100 bases:

>100_bases
GCGAGAACATGGCCTATGAATGTCTGAAATATAGCAATGAAAATATTAACAGGGCTCTGGAAGCCCTGCGTGCCGGAAAA
ATGATCCAGATCTATGACTC

Product: winged helix-turn-helix domain-containing protein/riboflavin kinase

Products: NA

Alternate protein names: RFK; CTP-dependent riboflavin kinase; CTP:riboflavin 5'-phosphotransferase; Flavokinase [H]

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MPDIEYLKKLALIGAVNKTVKVSSSEFHKHTGDSSKTAARKLKQLEKERLIERELVPGGQLIKMTEKGIEILKNEYVEYS
RIFSSEPDTLELEGNVLKGLGEGQYYINIPGYRTQFEEKLHFIPFPGTLNVQLSENSSALRNRLREMPSVRIDGFNDGER
TFGGGNCYPVRVEGIDAAVVVPDRTHYPSDLIEIIAPVKLRDALKLKDGDRVVTQLKKQGMEGQK

Sequences:

>Translated_225_residues
MPDIEYLKKLALIGAVNKTVKVSSSEFHKHTGDSSKTAARKLKQLEKERLIERELVPGGQLIKMTEKGIEILKNEYVEYS
RIFSSEPDTLELEGNVLKGLGEGQYYINIPGYRTQFEEKLHFIPFPGTLNVQLSENSSALRNRLREMPSVRIDGFNDGER
TFGGGNCYPVRVEGIDAAVVVPDRTHYPSDLIEIIAPVKLRDALKLKDGDRVVTQLKKQGMEGQK
>Mature_224_residues
PDIEYLKKLALIGAVNKTVKVSSSEFHKHTGDSSKTAARKLKQLEKERLIERELVPGGQLIKMTEKGIEILKNEYVEYSR
IFSSEPDTLELEGNVLKGLGEGQYYINIPGYRTQFEEKLHFIPFPGTLNVQLSENSSALRNRLREMPSVRIDGFNDGERT
FGGGNCYPVRVEGIDAAVVVPDRTHYPSDLIEIIAPVKLRDALKLKDGDRVVTQLKKQGMEGQK

Specific function: Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) [H]

COG id: COG1339

COG function: function code KH; Transcriptional regulator of a riboflavin/FAD biosynthetic operon

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the archaeal riboflavin kinase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015865
- InterPro:   IPR002834
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01982 CTP-dep_RFKase [H]

EC number: =2.7.1.161 [H]

Molecular weight: Translated: 25317; Mature: 25186

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDIEYLKKLALIGAVNKTVKVSSSEFHKHTGDSSKTAARKLKQLEKERLIERELVPGGQ
CCCHHHHHHHHHHHHCCCEEEECCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
LIKMTEKGIEILKNEYVEYSRIFSSEPDTLELEGNVLKGLGEGQYYINIPGYRTQFEEKL
EEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEECCCCCEEEEECCCCHHHHHCCC
HFIPFPGTLNVQLSENSSALRNRLREMPSVRIDGFNDGERTFGGGNCYPVRVEGIDAAVV
EEEECCCEEEEEECCCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEEEECCCCEEEE
VPDRTHYPSDLIEIIAPVKLRDALKLKDGDRVVTQLKKQGMEGQK
CCCCCCCHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PDIEYLKKLALIGAVNKTVKVSSSEFHKHTGDSSKTAARKLKQLEKERLIERELVPGGQ
CCHHHHHHHHHHHHCCCEEEECCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
LIKMTEKGIEILKNEYVEYSRIFSSEPDTLELEGNVLKGLGEGQYYINIPGYRTQFEEKL
EEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEECCCCCEEEEECCCCHHHHHCCC
HFIPFPGTLNVQLSENSSALRNRLREMPSVRIDGFNDGERTFGGGNCYPVRVEGIDAAVV
EEEECCCEEEEEECCCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEEEECCCCEEEE
VPDRTHYPSDLIEIIAPVKLRDALKLKDGDRVVTQLKKQGMEGQK
CCCCCCCHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]