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The map label for this gene is hisF [H]

Identifier: 21227806

GI number: 21227806

Start: 2047680

End: 2048501

Strand: Reverse

Name: hisF [H]

Synonym: MM_1704

Alternate gene names: 21227806

Gene position: 2048501-2047680 (Counterclockwise)

Preceding gene: 21227807

Following gene: 21227805

Centisome position: 50.01

GC content: 49.03

Gene sequence:

>822_bases
ATGCTCACCAAAAGAATCATACCATGCCTTGATGTAACCCTTGACAGAGCAGGCGGCTGTGTCGTCAAGGGCGTGGAGTT
TGTGGACCTGAAAGAAGCCGGAGACCCTGTGGAGCTCGCCAAGCGTTATAATGAAGACGGTGCAGACGAGCTTGTTTTTC
TGGACATCACAGCTTCAGCTCACGGCAGGGAAACCATGATCGATGTAATAGAAAGGACTGCAGACGAGGTATTCATCCCC
CTTACAGTGGGGGGCGGCATCAGCTCGATTGAAGCTATCCGCCAGATCCTCAGAGCAGGTGCTGATAAGGTTTCTGTTAA
TACATCAGCAGTTAAAAATCCTGAATTTATCAAAGAATCTTCCGACATATTCGGAGCCCAGTGCATTGTTACTGCCATTG
ACTGCAAGCGAAATACCAATGTAAAGGACAATCCAGATAAGACCATTCTGGAGCTGGAAGACGGGACACCTGCCTGGTAT
GAAGTGGTAATTTACGGAGGCAGGAAAAACACGGGTATTGATGCAGTCCAGTGGGCAAAAAGAGCAGAGGAGCTGGGTTC
AGGGGAAATCCTGCTCACAAGTATGGACAGGGACGGAACCTGTGCAGGGTATGACATCCCTATCACAAGAAAACTCTCAG
AAGAACTTGATATCCCGATTATCGCCTCCGGAGGAGTGGGAAACCCCCAGCACATCTATGAAGGCTTTTCGGATGGAAAA
GCCGATGCCGCCCTTGCAGCAAGTATCTTCCACTTCGGGGAATATTCGATACAGGAAGTAAAAGAGTTCCTCAAGGAGAG
AAAAATCCCTGTCAGGCTCTGA

Upstream 100 bases:

>100_bases
TTCAGCAATTTCTGCCCCGTAAGCAGCCTGACCCTTTCCGGATTTCTGCCTGTTTAATTTCAATAAGCTTTTAATAACAG
TTAATTTCATTATCCCTTAG

Downstream 100 bases:

>100_bases
AATCCGAAAAACAGAATTTTTAGGATCTATCCGAACAGATTTCGGCCTGGCAGACTTATCCAGAATGGAGATTTCCAGAC
AGGAATTTTGTCCGATCCTA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MLTKRIIPCLDVTLDRAGGCVVKGVEFVDLKEAGDPVELAKRYNEDGADELVFLDITASAHGRETMIDVIERTADEVFIP
LTVGGGISSIEAIRQILRAGADKVSVNTSAVKNPEFIKESSDIFGAQCIVTAIDCKRNTNVKDNPDKTILELEDGTPAWY
EVVIYGGRKNTGIDAVQWAKRAEELGSGEILLTSMDRDGTCAGYDIPITRKLSEELDIPIIASGGVGNPQHIYEGFSDGK
ADAALAASIFHFGEYSIQEVKEFLKERKIPVRL

Sequences:

>Translated_273_residues
MLTKRIIPCLDVTLDRAGGCVVKGVEFVDLKEAGDPVELAKRYNEDGADELVFLDITASAHGRETMIDVIERTADEVFIP
LTVGGGISSIEAIRQILRAGADKVSVNTSAVKNPEFIKESSDIFGAQCIVTAIDCKRNTNVKDNPDKTILELEDGTPAWY
EVVIYGGRKNTGIDAVQWAKRAEELGSGEILLTSMDRDGTCAGYDIPITRKLSEELDIPIIASGGVGNPQHIYEGFSDGK
ADAALAASIFHFGEYSIQEVKEFLKERKIPVRL
>Mature_273_residues
MLTKRIIPCLDVTLDRAGGCVVKGVEFVDLKEAGDPVELAKRYNEDGADELVFLDITASAHGRETMIDVIERTADEVFIP
LTVGGGISSIEAIRQILRAGADKVSVNTSAVKNPEFIKESSDIFGAQCIVTAIDCKRNTNVKDNPDKTILELEDGTPAWY
EVVIYGGRKNTGIDAVQWAKRAEELGSGEILLTSMDRDGTCAGYDIPITRKLSEELDIPIIASGGVGNPQHIYEGFSDGK
ADAALAASIFHFGEYSIQEVKEFLKERKIPVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=277, Percent_Identity=42.2382671480144, Blast_Score=198, Evalue=4e-52,
Organism=Escherichia coli, GI87082028, Length=195, Percent_Identity=27.1794871794872, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=36.624203821656, Blast_Score=186, Evalue=3e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 29691; Mature: 29691

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTKRIIPCLDVTLDRAGGCVVKGVEFVDLKEAGDPVELAKRYNEDGADELVFLDITASA
CCCCHHCHHHHHHHCCCCCEEEECCEEEECCCCCCHHHHHHHCCCCCCCCEEEEEEECCC
HGRETMIDVIERTADEVFIPLTVGGGISSIEAIRQILRAGADKVSVNTSAVKNPEFIKES
CCHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEECHHHCCCCHHHHCC
SDIFGAQCIVTAIDCKRNTNVKDNPDKTILELEDGTPAWYEVVIYGGRKNTGIDAVQWAK
CCCCCCEEEEEEEECCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHH
RAEELGSGEILLTSMDRDGTCAGYDIPITRKLSEELDIPIIASGGVGNPQHIYEGFSDGK
HHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHCCCEEEECCCCCCHHHHHCCCCCCC
ADAALAASIFHFGEYSIQEVKEFLKERKIPVRL
HHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLTKRIIPCLDVTLDRAGGCVVKGVEFVDLKEAGDPVELAKRYNEDGADELVFLDITASA
CCCCHHCHHHHHHHCCCCCEEEECCEEEECCCCCCHHHHHHHCCCCCCCCEEEEEEECCC
HGRETMIDVIERTADEVFIPLTVGGGISSIEAIRQILRAGADKVSVNTSAVKNPEFIKES
CCHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEECHHHCCCCHHHHCC
SDIFGAQCIVTAIDCKRNTNVKDNPDKTILELEDGTPAWYEVVIYGGRKNTGIDAVQWAK
CCCCCCEEEEEEEECCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHH
RAEELGSGEILLTSMDRDGTCAGYDIPITRKLSEELDIPIIASGGVGNPQHIYEGFSDGK
HHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHCCCEEEECCCCCCHHHHHCCCCCCC
ADAALAASIFHFGEYSIQEVKEFLKERKIPVRL
HHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]