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The map label for this gene is flpA

Identifier: 21227696

GI number: 21227696

Start: 1898345

End: 1899028

Strand: Direct

Name: flpA

Synonym: MM_1594

Alternate gene names: 21227696

Gene position: 1898345-1899028 (Clockwise)

Preceding gene: 21227695

Following gene: 21227698

Centisome position: 46.34

GC content: 44.74

Gene sequence:

>684_bases
ATGCCCGAAATAAGGCAGCTTTCCGAAGGGATTTTTGAGGTTACAAAAGACAAAAAACAGCTTTCAACTCTTAACCTTGA
CCCGGGAAAAGTCGTTTACGGAGAAAAGCTGATTTCTGTTGAAGGAGATGAGTACAGGACATGGGACCCCCGCAGAAGCA
AACTCGGAGCCATGGTCCTCAAAAAATTTGATATCCCCCTGAAAAGGAATTCAAAGGTTCTTTATCTCGGGGCTGCTTCA
GGAACAACAGTAAGCCACGTATCGGATATCGTTTCCGAAGGAGCGGTGTATTCTGTTGAATTTGCCCCAAGAAGCATGCG
CGACTTCATAGGGCTTGCTTCCAGACGCAAAAATATTTTCCCTATTCTTGCAGATGCTGGAAAGCCGGACAGTTACGCAC
ATATTGTTGAGCCTGTAGATGTTATCTTTCAGGATGTTGCCCAGCCTAACCAGGCAGAGATAGCTGCAAGAAATGCTGTC
CGTTTTTTGAAAAAGGACGGATATCTCCTGCTTTCTATCAAGGCTCGCAGTATTGATACTGCAGCAAGCCCGAAGGAGAT
CTTCAAAGAAGAGGTAAAAAAGCTTGAACAGGCTTTCGAACCCGGATTCGAAGTTTTGACAGCAAGGGAACTTATGCCTT
ATCATGAAGACCATCTTGGAGTGATGGCCAGATTAAAGAAATAA

Upstream 100 bases:

>100_bases
ACTGGAAGAAAAGATCCGGAAGATCAGGGCTGAAAACCCCAGACCTCCCCAGAAGCGGCAGGAAATCAGGGCAAAGCCAA
AAAAGAAGAGGAGAAAATAA

Downstream 100 bases:

>100_bases
ACCCACTTAAATCTCACTTTTGAATCCTGCTCTTTCAGAAGGCTGAACCATTCCGCAACTTTTCAGGTCAGGACTTTTTG
AATGGTAGTATGAAGTTACT

Product: fibrillarin

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MPEIRQLSEGIFEVTKDKKQLSTLNLDPGKVVYGEKLISVEGDEYRTWDPRRSKLGAMVLKKFDIPLKRNSKVLYLGAAS
GTTVSHVSDIVSEGAVYSVEFAPRSMRDFIGLASRRKNIFPILADAGKPDSYAHIVEPVDVIFQDVAQPNQAEIAARNAV
RFLKKDGYLLLSIKARSIDTAASPKEIFKEEVKKLEQAFEPGFEVLTARELMPYHEDHLGVMARLKK

Sequences:

>Translated_227_residues
MPEIRQLSEGIFEVTKDKKQLSTLNLDPGKVVYGEKLISVEGDEYRTWDPRRSKLGAMVLKKFDIPLKRNSKVLYLGAAS
GTTVSHVSDIVSEGAVYSVEFAPRSMRDFIGLASRRKNIFPILADAGKPDSYAHIVEPVDVIFQDVAQPNQAEIAARNAV
RFLKKDGYLLLSIKARSIDTAASPKEIFKEEVKKLEQAFEPGFEVLTARELMPYHEDHLGVMARLKK
>Mature_226_residues
PEIRQLSEGIFEVTKDKKQLSTLNLDPGKVVYGEKLISVEGDEYRTWDPRRSKLGAMVLKKFDIPLKRNSKVLYLGAASG
TTVSHVSDIVSEGAVYSVEFAPRSMRDFIGLASRRKNIFPILADAGKPDSYAHIVEPVDVIFQDVAQPNQAEIAARNAVR
FLKKDGYLLLSIKARSIDTAASPKEIFKEEVKKLEQAFEPGFEVLTARELMPYHEDHLGVMARLKK

Specific function: Involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the sub

COG id: COG1889

COG function: function code J; Fibrillarin-like rRNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. Fibrillarin family

Homologues:

Organism=Homo sapiens, GI12056465, Length=221, Percent_Identity=51.5837104072398, Blast_Score=198, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17563672, Length=221, Percent_Identity=48.4162895927602, Blast_Score=189, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6320190, Length=228, Percent_Identity=44.7368421052632, Blast_Score=174, Evalue=1e-44,
Organism=Drosophila melanogaster, GI17647425, Length=221, Percent_Identity=51.131221719457, Blast_Score=192, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24646406, Length=198, Percent_Identity=36.3636363636364, Blast_Score=96, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FLPA_METMA (O53133)

Other databases:

- EMBL:   AE008384
- EMBL:   Y16231
- PIR:   T47220
- RefSeq:   NP_633618.1
- ProteinModelPortal:   O53133
- SMR:   O53133
- GeneID:   1479936
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_1594
- NMPDR:   fig|192952.1.peg.1594
- HOGENOM:   HBG297404
- OMA:   CAERENI
- ProtClustDB:   PRK04266
- BioCyc:   MMAZ192952:MM1594-MONOMER
- HAMAP:   MF_00351
- InterPro:   IPR000692
- InterPro:   IPR020813
- PANTHER:   PTHR10335
- PIRSF:   PIRSF006540
- PRINTS:   PR00052

Pfam domain/function: PF01269 Fibrillarin

EC number: NA

Molecular weight: Translated: 25450; Mature: 25318

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: PS00566 FIBRILLARIN

Important sites: BINDING 54-54 BINDING 145-145

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEIRQLSEGIFEVTKDKKQLSTLNLDPGKVVYGEKLISVEGDEYRTWDPRRSKLGAMVL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCEEEEECCCCCCCCCHHHHHHHHHHH
KKFDIPLKRNSKVLYLGAASGTTVSHVSDIVSEGAVYSVEFAPRSMRDFIGLASRRKNIF
HHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCCCC
PILADAGKPDSYAHIVEPVDVIFQDVAQPNQAEIAARNAVRFLKKDGYLLLSIKARSIDT
EEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCC
AASPKEIFKEEVKKLEQAFEPGFEVLTARELMPYHEDHLGVMARLKK
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
PEIRQLSEGIFEVTKDKKQLSTLNLDPGKVVYGEKLISVEGDEYRTWDPRRSKLGAMVL
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCEEEEECCCCCCCCCHHHHHHHHHHH
KKFDIPLKRNSKVLYLGAASGTTVSHVSDIVSEGAVYSVEFAPRSMRDFIGLASRRKNIF
HHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCCCC
PILADAGKPDSYAHIVEPVDVIFQDVAQPNQAEIAARNAVRFLKKDGYLLLSIKARSIDT
EEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCC
AASPKEIFKEEVKKLEQAFEPGFEVLTARELMPYHEDHLGVMARLKK
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824; 10925204