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The map label for this gene is opuE [H]

Identifier: 21227651

GI number: 21227651

Start: 1846662

End: 1848581

Strand: Reverse

Name: opuE [H]

Synonym: MM_1549

Alternate gene names: 21227651

Gene position: 1848581-1846662 (Counterclockwise)

Preceding gene: 21227653

Following gene: 21227649

Centisome position: 45.13

GC content: 47.81

Gene sequence:

>1920_bases
ATGGATGGTTATAATATCTTTTTGGCTTTGCTTGCAGTCTATATAATCGGACTTGTGACTGTTGGCTGGTACTTCAATAA
AAAACAGAAATCTATTACCGACTTCTGGCTTGCAGGACGAAAAGTAGGTCCCGGAGCACTTGGATTTTCAGCTGCAGCAT
CATGGCTTACAGCTGGAGGAATTCTTGCGGTTATCGGGTATTTCATACTGCAGGGAATGGGCTCCATATGGGCTTTTGTA
GCTCCAAATATTGTCGCCCTCCTTGTCATCGCTGTACTTGTGGGCAAGATCAAAAACCTGCCTGCAATTACACAGCCTGA
ACTTCTGGAACAGCGTTATGGGAGCTCAGTCCGGTGGCCTGTTGCTCTGATCATTACCGTAGTGATGATTCTTTTTGCAG
TAGCAGATGTTACAGGTCTCTCCCTCGTGCTTCAGGTATTTTACGGGCTTGATCCGCTCTATGCTGCGGCTATTGTTGCA
ATTGCAGTATCCCTTTACGTAACCCTCGGCGGCCTTTCGGCAGTAGTCTGGACAGATGTCATCCAGTTCTCGTTCCTCTC
TCTTTTTACAATAGCAATGGCTTTTGCTGCAGTAGGGACAGTGGCAGATGGAGGTCTTAATACTCCTGCAATAAGCAGCT
CTGAACTCTTCGGGAGCCTTCCCGATGTATGGTGGAACCCCTTGTCAATCGGCCTCCCCATGGTTCTGATCTTCTGCCTT
GCCATTATTCCGGGATGGATTACAGAACAGGACCCCTGGCAGAAGGTATGGGCTGCAAGAGATGCAAAATCCGCCCGTAC
AGGGATGATCATAGGGTCTCTTATGATCACCATAGTTTTCTCGGCATGTGCTGTAATAGCAATAGCTCTCAGTTCCATAT
ACCCGGAAATCCCGCAAATGGGCTTCCCTGCAGGCATGGCACAGGCAGAGCCTGCACTTCTTGCTTTTGTCAGTGAGCGT
TTTGCTTCTGCTCCCCTGATTGTAGCTATAAGTGCAATAGGGCTTGCAGCAGCTTCCATGTCCTGCGCTGACACCTTTGC
AACATCTGGAGCTTCCTGCATTTCAAGGGACATTTATCAAAGGTACATAAAGCCCGATGCTACAATGAAGGAGATGCTCA
TTGTAAACAGGGTGAGTGTGCTAATTATCGTACTTGCAGCAACAGCTGGCTCATTTATTATCCCCAACATTATAGATGCA
ATTCATATTGCTACCTTTATTGCAAGCGCTTCTTACTTCTTTCCTCTTATGGGAGGGCTCTACTGGAAAAGAGCGACAAA
AGAAGGAGCTTTTGCAGGGATGGTAATCGGATTTATTGTCCAGGTAGCCCTTGTAGTTCTGGACCTTGTAAAAACCCCTC
CCCTGGCTCCAAGCTACCTTGAGACCATCCATCCCGTTCTGATGAGCCACGGTGTTATTGTGGGAATGAGCCTGAGTGCA
ATTGCATTCTTTGGGGTTTCCCTGATGACAAAACCCTCAAGCAGGGTTAACCTTGCACCCTTTTTCGAAGAAGAAGCAGA
AGAGCTTGCCCGCTATGAGACAGGTGAGATCAACGAAGCAGATCCATCGTACAGCGCATTCCTCAAAAACGTTAATGAGA
AAGTTGCAGGGGAGCGTACTCACCTTCAGCTTAACCTCAGGACTTCAGGAACCCTCAGCTGGCACGAGCTTGTAGAAAAG
CTGAAAAGCAGTTCTCCTGCATGGGTTACTCCTACGGGGCTTGACTCCGTATACAGGTTGACCCACGGAGACATGCTCGC
CTGCGTGGCTGTTACCAGAGGAAGCGGAGAACAGGACATCTGGCTCAAAGCCGAACCAAAAATAGAAACCGTAGGAAGGC
AGAGAAAAGAGCTTTTTACAGCCTATGAAGAGCTGAAGAAAGTGCTTGACCAGAAAGGAGTTAAACTGGATTTTTACTGA

Upstream 100 bases:

>100_bases
TATAAAAACTAAGTACTCCTGAATAATTGTATTAATTTCGGAAAAATTTCTATAATCAGATACTACTCATACACATTATG
ATTCTTTTAAAGGTGTCCTT

Downstream 100 bases:

>100_bases
CTTAAATTTGAATACTTAAGACCGGGGTTCCTTACAGAACCCTGCCTTAATTTCTTTTTTTGGAACTTAACATTTCAGGA
ATACAGAGCTTTTTCAATGA

Product: sodium/proline symporter

Products: NA

Alternate protein names: Sodium/proline symporter [H]

Number of amino acids: Translated: 639; Mature: 639

Protein sequence:

>639_residues
MDGYNIFLALLAVYIIGLVTVGWYFNKKQKSITDFWLAGRKVGPGALGFSAAASWLTAGGILAVIGYFILQGMGSIWAFV
APNIVALLVIAVLVGKIKNLPAITQPELLEQRYGSSVRWPVALIITVVMILFAVADVTGLSLVLQVFYGLDPLYAAAIVA
IAVSLYVTLGGLSAVVWTDVIQFSFLSLFTIAMAFAAVGTVADGGLNTPAISSSELFGSLPDVWWNPLSIGLPMVLIFCL
AIIPGWITEQDPWQKVWAARDAKSARTGMIIGSLMITIVFSACAVIAIALSSIYPEIPQMGFPAGMAQAEPALLAFVSER
FASAPLIVAISAIGLAAASMSCADTFATSGASCISRDIYQRYIKPDATMKEMLIVNRVSVLIIVLAATAGSFIIPNIIDA
IHIATFIASASYFFPLMGGLYWKRATKEGAFAGMVIGFIVQVALVVLDLVKTPPLAPSYLETIHPVLMSHGVIVGMSLSA
IAFFGVSLMTKPSSRVNLAPFFEEEAEELARYETGEINEADPSYSAFLKNVNEKVAGERTHLQLNLRTSGTLSWHELVEK
LKSSSPAWVTPTGLDSVYRLTHGDMLACVAVTRGSGEQDIWLKAEPKIETVGRQRKELFTAYEELKKVLDQKGVKLDFY

Sequences:

>Translated_639_residues
MDGYNIFLALLAVYIIGLVTVGWYFNKKQKSITDFWLAGRKVGPGALGFSAAASWLTAGGILAVIGYFILQGMGSIWAFV
APNIVALLVIAVLVGKIKNLPAITQPELLEQRYGSSVRWPVALIITVVMILFAVADVTGLSLVLQVFYGLDPLYAAAIVA
IAVSLYVTLGGLSAVVWTDVIQFSFLSLFTIAMAFAAVGTVADGGLNTPAISSSELFGSLPDVWWNPLSIGLPMVLIFCL
AIIPGWITEQDPWQKVWAARDAKSARTGMIIGSLMITIVFSACAVIAIALSSIYPEIPQMGFPAGMAQAEPALLAFVSER
FASAPLIVAISAIGLAAASMSCADTFATSGASCISRDIYQRYIKPDATMKEMLIVNRVSVLIIVLAATAGSFIIPNIIDA
IHIATFIASASYFFPLMGGLYWKRATKEGAFAGMVIGFIVQVALVVLDLVKTPPLAPSYLETIHPVLMSHGVIVGMSLSA
IAFFGVSLMTKPSSRVNLAPFFEEEAEELARYETGEINEADPSYSAFLKNVNEKVAGERTHLQLNLRTSGTLSWHELVEK
LKSSSPAWVTPTGLDSVYRLTHGDMLACVAVTRGSGEQDIWLKAEPKIETVGRQRKELFTAYEELKKVLDQKGVKLDFY
>Mature_639_residues
MDGYNIFLALLAVYIIGLVTVGWYFNKKQKSITDFWLAGRKVGPGALGFSAAASWLTAGGILAVIGYFILQGMGSIWAFV
APNIVALLVIAVLVGKIKNLPAITQPELLEQRYGSSVRWPVALIITVVMILFAVADVTGLSLVLQVFYGLDPLYAAAIVA
IAVSLYVTLGGLSAVVWTDVIQFSFLSLFTIAMAFAAVGTVADGGLNTPAISSSELFGSLPDVWWNPLSIGLPMVLIFCL
AIIPGWITEQDPWQKVWAARDAKSARTGMIIGSLMITIVFSACAVIAIALSSIYPEIPQMGFPAGMAQAEPALLAFVSER
FASAPLIVAISAIGLAAASMSCADTFATSGASCISRDIYQRYIKPDATMKEMLIVNRVSVLIIVLAATAGSFIIPNIIDA
IHIATFIASASYFFPLMGGLYWKRATKEGAFAGMVIGFIVQVALVVLDLVKTPPLAPSYLETIHPVLMSHGVIVGMSLSA
IAFFGVSLMTKPSSRVNLAPFFEEEAEELARYETGEINEADPSYSAFLKNVNEKVAGERTHLQLNLRTSGTLSWHELVEK
LKSSSPAWVTPTGLDSVYRLTHGDMLACVAVTRGSGEQDIWLKAEPKIETVGRQRKELFTAYEELKKVLDQKGVKLDFY

Specific function: Catalyzes the sodium-dependent uptake of extracellular proline [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI109659836, Length=445, Percent_Identity=22.9213483146067, Blast_Score=84, Evalue=6e-16,
Organism=Homo sapiens, GI14140236, Length=640, Percent_Identity=21.875, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI109659839, Length=459, Percent_Identity=23.5294117647059, Blast_Score=79, Evalue=2e-14,
Organism=Homo sapiens, GI206597487, Length=477, Percent_Identity=22.6415094339623, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI206597483, Length=477, Percent_Identity=22.6415094339623, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI17941285, Length=556, Percent_Identity=24.8201438848921, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI11141885, Length=425, Percent_Identity=24.7058823529412, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1790503, Length=467, Percent_Identity=23.5546038543897, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1787251, Length=460, Percent_Identity=25.6521739130435, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI87082237, Length=472, Percent_Identity=21.8220338983051, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17539284, Length=480, Percent_Identity=22.7083333333333, Blast_Score=69, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321771, Length=580, Percent_Identity=23.9655172413793, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24650192, Length=473, Percent_Identity=23.0443974630021, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28573698, Length=447, Percent_Identity=23.7136465324385, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 68780; Mature: 68780

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00457 NA_SOLUT_SYMP_2 ; PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGYNIFLALLAVYIIGLVTVGWYFNKKQKSITDFWLAGRKVGPGALGFSAAASWLTAGG
CCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCC
ILAVIGYFILQGMGSIWAFVAPNIVALLVIAVLVGKIKNLPAITQPELLEQRYGSSVRWP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHH
VALIITVVMILFAVADVTGLSLVLQVFYGLDPLYAAAIVAIAVSLYVTLGGLSAVVWTDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQFSFLSLFTIAMAFAAVGTVADGGLNTPAISSSELFGSLPDVWWNPLSIGLPMVLIFCL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCHHHCCCHHCCHHHHHHHHH
AIIPGWITEQDPWQKVWAARDAKSARTGMIIGSLMITIVFSACAVIAIALSSIYPEIPQM
HHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GFPAGMAQAEPALLAFVSERFASAPLIVAISAIGLAAASMSCADTFATSGASCISRDIYQ
CCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
RYIKPDATMKEMLIVNRVSVLIIVLAATAGSFIIPNIIDAIHIATFIASASYFFPLMGGL
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YWKRATKEGAFAGMVIGFIVQVALVVLDLVKTPPLAPSYLETIHPVLMSHGVIVGMSLSA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHEEHHHHH
IAFFGVSLMTKPSSRVNLAPFFEEEAEELARYETGEINEADPSYSAFLKNVNEKVAGERT
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCE
HLQLNLRTSGTLSWHELVEKLKSSSPAWVTPTGLDSVYRLTHGDMLACVAVTRGSGEQDI
EEEEEECCCCCCHHHHHHHHHHCCCCCEECCCCHHHHHHHCCCCEEEEEEEECCCCCCCE
WLKAEPKIETVGRQRKELFTAYEELKKVLDQKGVKLDFY
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCC
>Mature Secondary Structure
MDGYNIFLALLAVYIIGLVTVGWYFNKKQKSITDFWLAGRKVGPGALGFSAAASWLTAGG
CCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCC
ILAVIGYFILQGMGSIWAFVAPNIVALLVIAVLVGKIKNLPAITQPELLEQRYGSSVRWP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHH
VALIITVVMILFAVADVTGLSLVLQVFYGLDPLYAAAIVAIAVSLYVTLGGLSAVVWTDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQFSFLSLFTIAMAFAAVGTVADGGLNTPAISSSELFGSLPDVWWNPLSIGLPMVLIFCL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCHHHCCCHHCCHHHHHHHHH
AIIPGWITEQDPWQKVWAARDAKSARTGMIIGSLMITIVFSACAVIAIALSSIYPEIPQM
HHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GFPAGMAQAEPALLAFVSERFASAPLIVAISAIGLAAASMSCADTFATSGASCISRDIYQ
CCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
RYIKPDATMKEMLIVNRVSVLIIVLAATAGSFIIPNIIDAIHIATFIASASYFFPLMGGL
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YWKRATKEGAFAGMVIGFIVQVALVVLDLVKTPPLAPSYLETIHPVLMSHGVIVGMSLSA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHEEHHHHH
IAFFGVSLMTKPSSRVNLAPFFEEEAEELARYETGEINEADPSYSAFLKNVNEKVAGERT
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCE
HLQLNLRTSGTLSWHELVEKLKSSSPAWVTPTGLDSVYRLTHGDMLACVAVTRGSGEQDI
EEEEEECCCCCCHHHHHHHHHHCCCCCEECCCCHHHHHHHCCCCEEEEEEEECCCCCCCE
WLKAEPKIETVGRQRKELFTAYEELKKVLDQKGVKLDFY
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377 [H]