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The map label for this gene is purP

Identifier: 21227595

GI number: 21227595

Start: 1780598

End: 1781668

Strand: Reverse

Name: purP

Synonym: MM_1493

Alternate gene names: 21227595

Gene position: 1781668-1780598 (Counterclockwise)

Preceding gene: 21227598

Following gene: 21227593

Centisome position: 43.49

GC content: 46.5

Gene sequence:

>1071_bases
ATGATCACAAAACAGCAGGTTCTTGAATTTCTGAAAAATTACGATTTAGATAACATCACTATTGCAACAGTCTGTTCTCA
CTCAAGCCTCCAGATCTTTGATGGGGCGAGAAAAGAAGGCTTCAGAACTATGGGGATCTGCGTTGGTAAACCTCCCAAGT
TCTATGAGGCATTCCCCAGAGCAAAACCTGATGAGTACCTTATTGTCGAAAGCTATGAAGACATAATGAACAAAGTTGAA
GAGCTCAGGAAAAAGAATGTAATAATTATTCCTCACGGTTCATTTGTTGCGTATCTGGGTACGGAAAATTTCGCAGAGAT
GGCTGTACCTACCTTCGGGAACCGGGCTGTACTGGAGTGGGAATCAGACAGGAGCAAGGAACGGGAATGGCTGCTTGGAG
CAGGGATCCATATGCCCGGAAAAATAGATGACCCTCGAGACATCAACGGACCTGTAATGGTCAAATACGACGGGGCAAAA
GGAGGAAAAGGATTCTTCGTTGCGAAAACCTATGAAGAGTTCGATGAGCTCGTAGACCGCACCCAGAAATATACGATTCA
GGAATTCATTACCGGAACCCGCTACTATCTTCACTACTTCTACTCCCCTATCAGGAACGAAGGGTATACATTAAGCGAAG
GCAGCCTTGAGCTCCTCAGCATGGACCGCAGGGTAGAGTCCAATGCTGATGAAATCTTCAGGCTCGGTTCCCCCAGAGAA
CTCATAGAAGCAGGAATTCGCCCGACATACGTGGTTACAGGAAACGTGCCCCTTGTTGCGAGGGAATCCCTCCTGCCCCT
GATCTTCTCTCTTGGAGAAAGAGTGGTCGAAGAGTCCCTTGGCCTTTTCGGCGGGATGATAGGGGCTTTCTGCCTTGAAA
CTGTTTTTACTGACGAGCTTGAAATTAAGGTATTTGAGATTTCCGCCAGAATTGTTGCAGGAACGAACCTTTACATTTCA
GGTTCCCCCTATGCAGATCTTATTCAGGAAGACCTCTCAACCGGAAGGAGAATAGCGCAGGAAATCAAAGAGGCAGTCCG
GAAAAACCAGCTGGATAAAATAATATCCTGA

Upstream 100 bases:

>100_bases
GTTATTATAGATCCACTACTATATTTATATATAGAATCCTGCCCTTAAGCTTAAATATAGCATTGTATATTCTAATCTAA
ATTTTTCGCTGGTAGCAATC

Downstream 100 bases:

>100_bases
GCTTCCGGGTGTCCTGAATCTGCTGAAAAATCCTCAGCAGCTTTCAGGGAATTGATTTTTTGTAAATTCGGATCTGACTG
ACTCAGATTTGATTTTTAGG

Product: 5-formaminoimidazole-4-carboxamide-1-(beta)-D- ribofuranosyl 5'-monophosphate synthetase

Products: NA

Alternate protein names: 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl 5'-monophosphate--formate ligase 1

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MITKQQVLEFLKNYDLDNITIATVCSHSSLQIFDGARKEGFRTMGICVGKPPKFYEAFPRAKPDEYLIVESYEDIMNKVE
ELRKKNVIIIPHGSFVAYLGTENFAEMAVPTFGNRAVLEWESDRSKEREWLLGAGIHMPGKIDDPRDINGPVMVKYDGAK
GGKGFFVAKTYEEFDELVDRTQKYTIQEFITGTRYYLHYFYSPIRNEGYTLSEGSLELLSMDRRVESNADEIFRLGSPRE
LIEAGIRPTYVVTGNVPLVARESLLPLIFSLGERVVEESLGLFGGMIGAFCLETVFTDELEIKVFEISARIVAGTNLYIS
GSPYADLIQEDLSTGRRIAQEIKEAVRKNQLDKIIS

Sequences:

>Translated_356_residues
MITKQQVLEFLKNYDLDNITIATVCSHSSLQIFDGARKEGFRTMGICVGKPPKFYEAFPRAKPDEYLIVESYEDIMNKVE
ELRKKNVIIIPHGSFVAYLGTENFAEMAVPTFGNRAVLEWESDRSKEREWLLGAGIHMPGKIDDPRDINGPVMVKYDGAK
GGKGFFVAKTYEEFDELVDRTQKYTIQEFITGTRYYLHYFYSPIRNEGYTLSEGSLELLSMDRRVESNADEIFRLGSPRE
LIEAGIRPTYVVTGNVPLVARESLLPLIFSLGERVVEESLGLFGGMIGAFCLETVFTDELEIKVFEISARIVAGTNLYIS
GSPYADLIQEDLSTGRRIAQEIKEAVRKNQLDKIIS
>Mature_356_residues
MITKQQVLEFLKNYDLDNITIATVCSHSSLQIFDGARKEGFRTMGICVGKPPKFYEAFPRAKPDEYLIVESYEDIMNKVE
ELRKKNVIIIPHGSFVAYLGTENFAEMAVPTFGNRAVLEWESDRSKEREWLLGAGIHMPGKIDDPRDINGPVMVKYDGAK
GGKGFFVAKTYEEFDELVDRTQKYTIQEFITGTRYYLHYFYSPIRNEGYTLSEGSLELLSMDRRVESNADEIFRLGSPRE
LIEAGIRPTYVVTGNVPLVARESLLPLIFSLGERVVEESLGLFGGMIGAFCLETVFTDELEIKVFEISARIVAGTNLYIS
GSPYADLIQEDLSTGRRIAQEIKEAVRKNQLDKIIS

Specific function: Catalyzes the ATP- and formate-dependent formylation of 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'- monophosphate (AICAR) to 5-formaminoimidazole-4-carboxamide-1- beta-d-ribofuranosyl 5'-monophosphate (FAICAR) in the absence of folates

COG id: COG1759

COG function: function code R; ATP-utilizing enzymes of ATP-grasp superfamily (probably carboligases)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURP1_METMA (Q8PWT3)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_633517.1
- ProteinModelPortal:   Q8PWT3
- SMR:   Q8PWT3
- GeneID:   1479835
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_1493
- NMPDR:   fig|192952.1.peg.1493
- HOGENOM:   HBG392202
- OMA:   VFEISAR
- ProtClustDB:   PRK13278
- BioCyc:   MMAZ192952:MM1493-MONOMER
- HAMAP:   MF_01163
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR009720
- InterPro:   IPR010672
- InterPro:   IPR013817
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20

Pfam domain/function: PF06849 DUF1246; PF06973 DUF1297

EC number: NA

Molecular weight: Translated: 40283; Mature: 40283

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 27-27 BINDING 94-94 BINDING 226-226 BINDING 255-255

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITKQQVLEFLKNYDLDNITIATVCSHSSLQIFDGARKEGFRTMGICVGKPPKFYEAFPR
CCCHHHHHHHHHCCCCCCEEEEEEECCCCEEEECCHHHCCCEEEEEEECCCCHHHHHCCC
AKPDEYLIVESYEDIMNKVEELRKKNVIIIPHGSFVAYLGTENFAEMAVPTFGNRAVLEW
CCCCCEEEEECHHHHHHHHHHHHHCCEEEEECCCEEEEECCCCHHHHCCCCCCCEEEEEE
ESDRSKEREWLLGAGIHMPGKIDDPRDINGPVMVKYDGAKGGKGFFVAKTYEEFDELVDR
CCCCCCCHHEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEHHHHHHHHHHH
TQKYTIQEFITGTRYYLHYFYSPIRNEGYTLSEGSLELLSMDRRVESNADEIFRLGSPRE
HHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCHHHHHCCCCCHH
LIEAGIRPTYVVTGNVPLVARESLLPLIFSLGERVVEESLGLFGGMIGAFCLETVFTDEL
HHHCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
EIKVFEISARIVAGTNLYISGSPYADLIQEDLSTGRRIAQEIKEAVRKNQLDKIIS
EEEEEEEEEEEEECCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MITKQQVLEFLKNYDLDNITIATVCSHSSLQIFDGARKEGFRTMGICVGKPPKFYEAFPR
CCCHHHHHHHHHCCCCCCEEEEEEECCCCEEEECCHHHCCCEEEEEEECCCCHHHHHCCC
AKPDEYLIVESYEDIMNKVEELRKKNVIIIPHGSFVAYLGTENFAEMAVPTFGNRAVLEW
CCCCCEEEEECHHHHHHHHHHHHHCCEEEEECCCEEEEECCCCHHHHCCCCCCCEEEEEE
ESDRSKEREWLLGAGIHMPGKIDDPRDINGPVMVKYDGAKGGKGFFVAKTYEEFDELVDR
CCCCCCCHHEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEHHHHHHHHHHH
TQKYTIQEFITGTRYYLHYFYSPIRNEGYTLSEGSLELLSMDRRVESNADEIFRLGSPRE
HHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCHHHHHCCCCCHH
LIEAGIRPTYVVTGNVPLVARESLLPLIFSLGERVVEESLGLFGGMIGAFCLETVFTDEL
HHHCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
EIKVFEISARIVAGTNLYISGSPYADLIQEDLSTGRRIAQEIKEAVRKNQLDKIIS
EEEEEEEEEEEEECCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12125824