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The map label for this gene is mtmC [H]

Identifier: 21227540

GI number: 21227540

Start: 1718688

End: 1719494

Strand: Reverse

Name: mtmC [H]

Synonym: MM_1438

Alternate gene names: 21227540

Gene position: 1719494-1718688 (Counterclockwise)

Preceding gene: 21227541

Following gene: 21227539

Centisome position: 41.98

GC content: 47.46

Gene sequence:

>807_bases
ATGCGAACGCTTTTTTACTTAGCGGAGCTTGGGCTTGTGTCCAGCATAAACCTTTGCAATCTATTGTCTATTGAAGCTCT
GCGTACATTGTACTGCCCAACGATACTGCAAAGAAAAGCCGTTTTCGACAGAAACGGAGGTTTAGTAAAAATGGCAAACC
AAGAGATTTTTGACAAGTTAACCAATGCGATCGTAACTCAGGACATCGCAGGCTGCGCAAAGCTGACTCAAGAAGCCCTG
GATGCAGGAATTTCCCCTCTTGACATTATCACAAAGGGCCTTTCCCCAGGAATGAAGATTATCGGTGACAAGTTCGAAGC
TGCAGAGATCTTTCTGCCTCAGATCATGATGTCCGGAAAAGCCATGAGCAGCGCAATGGAAATCCTTACCCCCGAGCTTG
AGAAGACCAAAGTAGAAGGAGAGGAAGGAACCGGGCTTGCAATCACCTTTGTTGCAGAAGGAGACATCCACGATATCGGT
CACCGCCTTGTTACAACCATGCTCGGAGCAAACGGGTTTGATATCCTTGACCTTGGAGTAGACGTCCTTAACGAGACTGT
TATTGAAGAGGCTGCAAAGCGCAAGGGACAGAAAATCATTCTTGTTGGCTCAGCCCTTATGACCACCTCCATGCTCGGCC
AGAAAGACCTCATGGACAGGCTCAGGGAAGAAAACCTCAGGGACAGCGTAAAATGCATGTTCGGAGGAGCACCTGTATCC
GACAAATGGATTGATGAAATCGGTGCGGACGCAACCGCAGAAAACGCTGCAGAAGCTGCAAAAGTTGCACTTAACATTAT
GAAATAA

Upstream 100 bases:

>100_bases
TGGATAATTTCCGATATAGACAATCCAGAATACTGTGAAGGCTTTCCCGCGTTAAATGGCTGAATGAAAGCGCAGGACCT
GCACATAAACAAAGAACCCC

Downstream 100 bases:

>100_bases
ACCTGGGAGGCAAAAGCACATGACATTCAGAAAATCATTTGATTGCTACGACTTCTACGACAGGGCAAAAGTAGGAGAGA
AGTGCACCCAAGACGACTGG

Product: monomethylamine corrinoid protein

Products: L-Methionine; 5,6,7,8-Tetrahydrofolate [C]

Alternate protein names: MMCP 2 [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MRTLFYLAELGLVSSINLCNLLSIEALRTLYCPTILQRKAVFDRNGGLVKMANQEIFDKLTNAIVTQDIAGCAKLTQEAL
DAGISPLDIITKGLSPGMKIIGDKFEAAEIFLPQIMMSGKAMSSAMEILTPELEKTKVEGEEGTGLAITFVAEGDIHDIG
HRLVTTMLGANGFDILDLGVDVLNETVIEEAAKRKGQKIILVGSALMTTSMLGQKDLMDRLREENLRDSVKCMFGGAPVS
DKWIDEIGADATAENAAEAAKVALNIMK

Sequences:

>Translated_268_residues
MRTLFYLAELGLVSSINLCNLLSIEALRTLYCPTILQRKAVFDRNGGLVKMANQEIFDKLTNAIVTQDIAGCAKLTQEAL
DAGISPLDIITKGLSPGMKIIGDKFEAAEIFLPQIMMSGKAMSSAMEILTPELEKTKVEGEEGTGLAITFVAEGDIHDIG
HRLVTTMLGANGFDILDLGVDVLNETVIEEAAKRKGQKIILVGSALMTTSMLGQKDLMDRLREENLRDSVKCMFGGAPVS
DKWIDEIGADATAENAAEAAKVALNIMK
>Mature_268_residues
MRTLFYLAELGLVSSINLCNLLSIEALRTLYCPTILQRKAVFDRNGGLVKMANQEIFDKLTNAIVTQDIAGCAKLTQEAL
DAGISPLDIITKGLSPGMKIIGDKFEAAEIFLPQIMMSGKAMSSAMEILTPELEKTKVEGEEGTGLAITFVAEGDIHDIG
HRLVTTMLGANGFDILDLGVDVLNETVIEEAAKRKGQKIILVGSALMTTSMLGQKDLMDRLREENLRDSVKCMFGGAPVS
DKWIDEIGADATAENAAEAAKVALNIMK

Specific function: Acts as a methyl group carrier between mtmB and mtbA [H]

COG id: COG5012

COG function: function code R; Predicted cobalamin binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=149, Percent_Identity=36.241610738255, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1790450, Length=198, Percent_Identity=30.3030303030303, Blast_Score=84, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17535405, Length=136, Percent_Identity=35.2941176470588, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR003759
- InterPro:   IPR012741 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2 [H]

EC number: 2.1.1.13 [C]

Molecular weight: Translated: 28893; Mature: 28893

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTLFYLAELGLVSSINLCNLLSIEALRTLYCPTILQRKAVFDRNGGLVKMANQEIFDKL
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHH
TNAIVTQDIAGCAKLTQEALDAGISPLDIITKGLSPGMKIIGDKFEAAEIFLPQIMMSGK
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHCCH
AMSSAMEILTPELEKTKVEGEEGTGLAITFVAEGDIHDIGHRLVTTMLGANGFDILDLGV
HHHHHHHHHCCCHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHH
DVLNETVIEEAAKRKGQKIILVGSALMTTSMLGQKDLMDRLREENLRDSVKCMFGGAPVS
HHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCC
DKWIDEIGADATAENAAEAAKVALNIMK
HHHHHHHCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRTLFYLAELGLVSSINLCNLLSIEALRTLYCPTILQRKAVFDRNGGLVKMANQEIFDKL
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHH
TNAIVTQDIAGCAKLTQEALDAGISPLDIITKGLSPGMKIIGDKFEAAEIFLPQIMMSGK
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHCCH
AMSSAMEILTPELEKTKVEGEEGTGLAITFVAEGDIHDIGHRLVTTMLGANGFDILDLGV
HHHHHHHHHCCCHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHH
DVLNETVIEEAAKRKGQKIILVGSALMTTSMLGQKDLMDRLREENLRDSVKCMFGGAPVS
HHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCC
DKWIDEIGADATAENAAEAAKVALNIMK
HHHHHHHCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP; Cobinamide; S-adenosyl-L-methionine [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 500-780 [C]

Specific activity: 3.8

Km value (mM): 0.035 {S-methyltetrahydrofolate}} 0.0016 {S-adenosyl-L-methionine}} 0.00065 {S-adenosyl-L-methionine}} 0.06 {methyltetrahydrofolate}} 0.03 {methyltetrahydrofolate}} 0.016 {homocysteine}} [C]

Substrates: 5-Methyltetrahydrofolate; L-Homocysteine [C]

Specific reaction: 5-Methyltetrahydrofolate + L-Homocysteine --> L-Methionine + 5,6,7,8-Tetrahydrofolate [C]

General reaction: Methyl group transfer [C]

Inhibitor: 5, 6-Dimethyl benzimidazole; alpha-Ribazole 3'-phosphate; Be2+; Cobinamide; Propyl iodide; Sr2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824 [H]