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The map label for this gene is 21227454

Identifier: 21227454

GI number: 21227454

Start: 1620523

End: 1621563

Strand: Direct

Name: 21227454

Synonym: MM_1352

Alternate gene names: NA

Gene position: 1620523-1621563 (Clockwise)

Preceding gene: 21227452

Following gene: 21227455

Centisome position: 39.56

GC content: 43.04

Gene sequence:

>1041_bases
ATGCAGGAGATCATTTTTATCGATGGAGGCAGTCTCCCAACACCTGAAGGTTTTACGAGGGAATGGGTGAAAGCTGCAGC
CGAGAGCCGAACTGAGGACCAAAAACTCTTTTCTCTGGTAAGTGACGCTTTTCAGAGAAAAATAGACGCTGGAGTTCATG
TTCCCACTTATCCGCAGTTTAGAGATATGATAGGACAGTTTCTTGACATTATCAGAGATGAGAAAAACTGTTATGAGCCC
TACGTCTTAAAAGAAGAGAACGCAAAAATCCTTGAGCTGGAGATAATTGATGAGGTTGCAAAACAGTACAGAGAAAGGAC
AGGCAAGACTCTGGAGGTTAGAGTTTGTGTTGCCGGTCCAACTGACCTTTACCTTCAGGCTTTCGGGGCAACGGCTTTCT
CAGATGCATACCATATCATGGCTCTGAATATTGAGAACTTCATAAAGCAGGCGTTTAAAGCTGCGAAAAACTTCAAAATT
AAGGTTATCGCCCTGGATGAACCAAGCCTTGGAATGAACGATAAAATTCAGTTTTCTGACTTAGATATTGTTTCTGCCCT
TACTCTGGCTTCCACTTACGCGCGGAAACAGGGGGCTGATGTGGAGATCCACCTGCACTCTCCTTTAAAGTACAAACTCA
TTTGCGAGACCCCTATCAACGTTATGGGGTTTGAATACGCTGCAACTCCTTCTTATATAGACCTTATGGACAAAAAAATT
CTGGAAGATTCGAATACTTATATAAGGCTCGGGGTCTCACGGACTGATATTTCAAGCCTCATAGGCATGGTTAACGAGAC
CTATGGAGTTAACGCCTGGAAAGAAAAAGAATATATGCAAAAAATCGTCACGGAACTTGAAACTCCTGATATAATACAAA
AAAGGCTTGAAAAAGCCTGCTCTATTCTTGGGGACCGTATAAAATACGCAAGTCCTGACTGCGGGCTAGCATTCTGGCCG
GACCAGGAGCTTGCTTTCAGATTGCTTGAAAATACGGCAAAAGGCATCAATGCATTTAATGCAGAGATGAAAGACCTCTA
A

Upstream 100 bases:

>100_bases
TATTATTCTTTTAAAAGTTTTATTTTATTATCTGTTCCTTAAGTTTATTATTCGGAGTAATTCCAATACTTATAATACTC
GATCAATCTTACATACAAAT

Downstream 100 bases:

>100_bases
TAGCTCCGGAAAGTTACAATCCAGGTAAATGAACAACTCCGGAAAATGGACAATTCAGGCAAATTAACAGCCCGGGTAGA
TGAATAACTCCGGAAGGGGG

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase

Products: tetrahydropteroyltri-L-glutamate; L-methionine

Alternate protein names: Methionine Synthase Vitamin-B12 Independent

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MQEIIFIDGGSLPTPEGFTREWVKAAAESRTEDQKLFSLVSDAFQRKIDAGVHVPTYPQFRDMIGQFLDIIRDEKNCYEP
YVLKEENAKILELEIIDEVAKQYRERTGKTLEVRVCVAGPTDLYLQAFGATAFSDAYHIMALNIENFIKQAFKAAKNFKI
KVIALDEPSLGMNDKIQFSDLDIVSALTLASTYARKQGADVEIHLHSPLKYKLICETPINVMGFEYAATPSYIDLMDKKI
LEDSNTYIRLGVSRTDISSLIGMVNETYGVNAWKEKEYMQKIVTELETPDIIQKRLEKACSILGDRIKYASPDCGLAFWP
DQELAFRLLENTAKGINAFNAEMKDL

Sequences:

>Translated_346_residues
MQEIIFIDGGSLPTPEGFTREWVKAAAESRTEDQKLFSLVSDAFQRKIDAGVHVPTYPQFRDMIGQFLDIIRDEKNCYEP
YVLKEENAKILELEIIDEVAKQYRERTGKTLEVRVCVAGPTDLYLQAFGATAFSDAYHIMALNIENFIKQAFKAAKNFKI
KVIALDEPSLGMNDKIQFSDLDIVSALTLASTYARKQGADVEIHLHSPLKYKLICETPINVMGFEYAATPSYIDLMDKKI
LEDSNTYIRLGVSRTDISSLIGMVNETYGVNAWKEKEYMQKIVTELETPDIIQKRLEKACSILGDRIKYASPDCGLAFWP
DQELAFRLLENTAKGINAFNAEMKDL
>Mature_346_residues
MQEIIFIDGGSLPTPEGFTREWVKAAAESRTEDQKLFSLVSDAFQRKIDAGVHVPTYPQFRDMIGQFLDIIRDEKNCYEP
YVLKEENAKILELEIIDEVAKQYRERTGKTLEVRVCVAGPTDLYLQAFGATAFSDAYHIMALNIENFIKQAFKAAKNFKI
KVIALDEPSLGMNDKIQFSDLDIVSALTLASTYARKQGADVEIHLHSPLKYKLICETPINVMGFEYAATPSYIDLMDKKI
LEDSNTYIRLGVSRTDISSLIGMVNETYGVNAWKEKEYMQKIVTELETPDIIQKRLEKACSILGDRIKYASPDCGLAFWP
DQELAFRLLENTAKGINAFNAEMKDL

Specific function: Unknown

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.1.1.14

Molecular weight: Translated: 39201; Mature: 39201

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEIIFIDGGSLPTPEGFTREWVKAAAESRTEDQKLFSLVSDAFQRKIDAGVHVPTYPQF
CCEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
RDMIGQFLDIIRDEKNCYEPYVLKEENAKILELEIIDEVAKQYRERTGKTLEVRVCVAGP
HHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEHHHHHHHHHHHHHHCCCEEEEEEEEECC
TDLYLQAFGATAFSDAYHIMALNIENFIKQAFKAAKNFKIKVIALDEPSLGMNDKIQFSD
HHHHHHHHCCHHHCCCEEEEEECHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEEECC
LDIVSALTLASTYARKQGADVEIHLHSPLKYKLICETPINVMGFEYAATPSYIDLMDKKI
HHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHHH
LEDSNTYIRLGVSRTDISSLIGMVNETYGVNAWKEKEYMQKIVTELETPDIIQKRLEKAC
HCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
SILGDRIKYASPDCGLAFWPDQELAFRLLENTAKGINAFNAEMKDL
HHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCHHHCC
>Mature Secondary Structure
MQEIIFIDGGSLPTPEGFTREWVKAAAESRTEDQKLFSLVSDAFQRKIDAGVHVPTYPQF
CCEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
RDMIGQFLDIIRDEKNCYEPYVLKEENAKILELEIIDEVAKQYRERTGKTLEVRVCVAGP
HHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEHHHHHHHHHHHHHHCCCEEEEEEEEECC
TDLYLQAFGATAFSDAYHIMALNIENFIKQAFKAAKNFKIKVIALDEPSLGMNDKIQFSD
HHHHHHHHCCHHHCCCEEEEEECHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEEECC
LDIVSALTLASTYARKQGADVEIHLHSPLKYKLICETPINVMGFEYAATPSYIDLMDKKI
HHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHHH
LEDSNTYIRLGVSRTDISSLIGMVNETYGVNAWKEKEYMQKIVTELETPDIIQKRLEKAC
HCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
SILGDRIKYASPDCGLAFWPDQELAFRLLENTAKGINAFNAEMKDL
HHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5-methyltetrahydropteroyltri-L-glutamate; L-homocysteine

Specific reaction: 5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA