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The map label for this gene is dusB [H]

Identifier: 21227445

GI number: 21227445

Start: 1606714

End: 1607685

Strand: Reverse

Name: dusB [H]

Synonym: MM_1343

Alternate gene names: 21227445

Gene position: 1607685-1606714 (Counterclockwise)

Preceding gene: 21227446

Following gene: 21227444

Centisome position: 39.25

GC content: 45.16

Gene sequence:

>972_bases
ATGAAACTTAAAAAACTGAAAATTGGCAGGACAGAGCTTTCTGGAAACCTCCTTCTCGCACCTATGGCAGATGTAACAAA
TCTGGCTTTCAGGCTGCTTTGCAGGAGGTATGGAGCTGACCTTACATATACCGAAATGATAAATGTAGATGCTTTGCTCA
ATGAAAGCAGAAAGTCTTTCATTAAAGGGCTGAGCTCTGAGGAGGACAGACCTTTTGGAGTTCAACTCGTAGGAGGGTGT
CCTGATAAGTTAAAAAGAGCTGCACTCTTTATTGAAGAAGAGTACAGGCCCGAGATTATTGATGTCAACATGGGCTGCCC
CGCAAGATGTATTACAGGCGCTGGCTGTGGTTCTGCCCTTCTCAACTCTCCGGAACTTGTATATTCAATAATCTTGGAGC
TGACAGAGTCACTTGACACTCCGGTCAGCGCAAAAATCAGACTCCTCGGAAGAGAGGAAAAAACGCTTGAAATTGCCCGC
CTGATAGAAAAGGCGGGGGCATCAGCTGTGACCGTGCACGGCAGGACGGCAGCACAGATGTATTCAGGTAACTCGAATCT
TGAGGGGATAAGAGCTGTCAAAAATGAACTTTCTATCCCGGTCATCGCAAACGGGGATGTAAAAGATGAAGAATCTGCAG
AAAGGACGTTAGAATTAACAGGCTGCGACGGACTCATGATAGGGCGTGCAGCAATGGGCAACCCATTTATATTCAAAAAA
ATCAGGCACTACCTGGAAACCGGCGAAAAGCTTGGAGCAGATAAGCCTTTCAGGCAGCTTGAGGATTTTAACTCTTATAT
TTCACTCCTTGAAGAACACAACCTGCTTTCATCCATAAACCTGAGAATGCACGCACACTGGTTTACCAAAGGACTTCGCG
GCTCGCGGCATGTCAGGGAAAAAATCAATGGTTTGGAAGATGGAAAAGCCATAACTGAACTGATAAGAAGTTTTTGCCAG
GAAATATATTAA

Upstream 100 bases:

>100_bases
TGAATATTAATCCGTAAGTATAAATGTCTCCAGAATGAAGAGAAACCTGAAATAAAAATCAAAGATAGAATATAAAAACC
AGTTCAAAATCGTAAGAAAC

Downstream 100 bases:

>100_bases
AATATCTCTTTTTTGGAGCAAAGCTTTGTTTACGGCAACAAGGAAAAAAAACGTTGTTAATCGGCAGGTTAACCTGAGAA
CAATAATTATAAAACACCTA

Product: NifR3-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MKLKKLKIGRTELSGNLLLAPMADVTNLAFRLLCRRYGADLTYTEMINVDALLNESRKSFIKGLSSEEDRPFGVQLVGGC
PDKLKRAALFIEEEYRPEIIDVNMGCPARCITGAGCGSALLNSPELVYSIILELTESLDTPVSAKIRLLGREEKTLEIAR
LIEKAGASAVTVHGRTAAQMYSGNSNLEGIRAVKNELSIPVIANGDVKDEESAERTLELTGCDGLMIGRAAMGNPFIFKK
IRHYLETGEKLGADKPFRQLEDFNSYISLLEEHNLLSSINLRMHAHWFTKGLRGSRHVREKINGLEDGKAITELIRSFCQ
EIY

Sequences:

>Translated_323_residues
MKLKKLKIGRTELSGNLLLAPMADVTNLAFRLLCRRYGADLTYTEMINVDALLNESRKSFIKGLSSEEDRPFGVQLVGGC
PDKLKRAALFIEEEYRPEIIDVNMGCPARCITGAGCGSALLNSPELVYSIILELTESLDTPVSAKIRLLGREEKTLEIAR
LIEKAGASAVTVHGRTAAQMYSGNSNLEGIRAVKNELSIPVIANGDVKDEESAERTLELTGCDGLMIGRAAMGNPFIFKK
IRHYLETGEKLGADKPFRQLEDFNSYISLLEEHNLLSSINLRMHAHWFTKGLRGSRHVREKINGLEDGKAITELIRSFCQ
EIY
>Mature_323_residues
MKLKKLKIGRTELSGNLLLAPMADVTNLAFRLLCRRYGADLTYTEMINVDALLNESRKSFIKGLSSEEDRPFGVQLVGGC
PDKLKRAALFIEEEYRPEIIDVNMGCPARCITGAGCGSALLNSPELVYSIILELTESLDTPVSAKIRLLGREEKTLEIAR
LIEKAGASAVTVHGRTAAQMYSGNSNLEGIRAVKNELSIPVIANGDVKDEESAERTLELTGCDGLMIGRAAMGNPFIFKK
IRHYLETGEKLGADKPFRQLEDFNSYISLLEEHNLLSSINLRMHAHWFTKGLRGSRHVREKINGLEDGKAITELIRSFCQ
EIY

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40807366, Length=320, Percent_Identity=32.1875, Blast_Score=151, Evalue=9e-37,
Organism=Homo sapiens, GI31742496, Length=230, Percent_Identity=35.2173913043478, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI239788462, Length=276, Percent_Identity=32.2463768115942, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI239788483, Length=263, Percent_Identity=31.9391634980989, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI8923374, Length=245, Percent_Identity=35.9183673469388, Blast_Score=122, Evalue=4e-28,
Organism=Escherichia coli, GI1789660, Length=321, Percent_Identity=38.3177570093458, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1788462, Length=239, Percent_Identity=35.9832635983264, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI145693211, Length=311, Percent_Identity=25.7234726688103, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI25144369, Length=227, Percent_Identity=36.5638766519824, Blast_Score=121, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17507177, Length=277, Percent_Identity=29.9638989169675, Blast_Score=120, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17543114, Length=249, Percent_Identity=32.1285140562249, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17510279, Length=256, Percent_Identity=32.8125, Blast_Score=110, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6323560, Length=291, Percent_Identity=31.6151202749141, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6323437, Length=227, Percent_Identity=29.9559471365639, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6323433, Length=266, Percent_Identity=27.0676691729323, Blast_Score=89, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24580595, Length=268, Percent_Identity=32.8358208955224, Blast_Score=147, Evalue=7e-36,
Organism=Drosophila melanogaster, GI19920448, Length=268, Percent_Identity=32.8358208955224, Blast_Score=147, Evalue=7e-36,
Organism=Drosophila melanogaster, GI19921524, Length=227, Percent_Identity=34.3612334801762, Blast_Score=128, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24585320, Length=258, Percent_Identity=34.4961240310078, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI45549423, Length=247, Percent_Identity=30.7692307692308, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35846; Mature: 35846

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKKLKIGRTELSGNLLLAPMADVTNLAFRLLCRRYGADLTYTEMINVDALLNESRKSF
CCCCCEECCCEECCCCEEEECHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
IKGLSSEEDRPFGVQLVGGCPDKLKRAALFIEEEYRPEIIDVNMGCPARCITGAGCGSAL
HHCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHCCCCCCHHH
LNSPELVYSIILELTESLDTPVSAKIRLLGREEKTLEIARLIEKAGASAVTVHGRTAAQM
HCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEECCCHHHHH
YSGNSNLEGIRAVKNELSIPVIANGDVKDEESAERTLELTGCDGLMIGRAAMGNPFIFKK
HCCCCCCHHHHHHHHHCCCEEEECCCCCCCHHHHHEEEEECCCCEEECHHHCCCHHHHHH
IRHYLETGEKLGADKPFRQLEDFNSYISLLEEHNLLSSINLRMHAHWFTKGLRGSRHVRE
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHCCCCHHHHHH
KINGLEDGKAITELIRSFCQEIY
HHCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLKKLKIGRTELSGNLLLAPMADVTNLAFRLLCRRYGADLTYTEMINVDALLNESRKSF
CCCCCEECCCEECCCCEEEECHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
IKGLSSEEDRPFGVQLVGGCPDKLKRAALFIEEEYRPEIIDVNMGCPARCITGAGCGSAL
HHCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHCCCCCCHHH
LNSPELVYSIILELTESLDTPVSAKIRLLGREEKTLEIARLIEKAGASAVTVHGRTAAQM
HCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEECCCHHHHH
YSGNSNLEGIRAVKNELSIPVIANGDVKDEESAERTLELTGCDGLMIGRAAMGNPFIFKK
HCCCCCCHHHHHHHHHCCCEEEECCCCCCCHHHHHEEEEECCCCEEECHHHCCCHHHHHH
IRHYLETGEKLGADKPFRQLEDFNSYISLLEEHNLLSSINLRMHAHWFTKGLRGSRHVRE
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHCCCCHHHHHH
KINGLEDGKAITELIRSFCQEIY
HHCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]