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The map label for this gene is cheB

Identifier: 21227428

GI number: 21227428

Start: 1581695

End: 1582798

Strand: Reverse

Name: cheB

Synonym: MM_1326

Alternate gene names: 21227428

Gene position: 1582798-1581695 (Counterclockwise)

Preceding gene: 21227429

Following gene: 21227427

Centisome position: 38.64

GC content: 44.38

Gene sequence:

>1104_bases
TTGTCAAACCAACATCCGAAGAAAGTGCTTGAAATGGTTATTCGTGCGCTCATAGTGGATGATTCTGCTTTGATTCGCAA
AGTCCTTTCCGATATTCTTAATCAGGATCCTAAAATCGCTGTTATCGGAACTGCAATCAATGGAGAGGACGGCCTTGAAA
AAGTCAGAAAACTTAAGCCCGATGTAGTTCTTCTTGATAATATAATGCCTGTTCTTGATGGGCTTAAGACTCTTTCCCAT
ATCATGGAAGAATTTCCGACTCCTGTGGTTATAGTGTCAGCTCTGGGGGAAAAGGCTGAGGAGATTACCCTCACAGCTCT
GGAGTATGGAGCAGTGGACGTAATCGAAAAGCCTTCGGGTATTCTCAGTCAAAGTATGCACGAGATGGCAGAAGAAATTT
GTGCAAAAGTCAGAGCAGTTTCAAAAGCCGATCTTAATAACCTTGGGTGCATCCGGGATTTAGAACACCAGATACCTGAA
AACCACAGGAAAAAAAAGAATAGCCTCCGGGAGAAAACCTCTGTTAGAAATGTGCTGGCTATAGGCGCATCTACAGGAGG
GCCAAGAGCTCTGGAAAAACTTATAGGCTCGTTTCCCGCTGAAATTCCAGCTGCGGTTCTTATAGTACAGCACATGCCTC
CGGGTTTTACAGCATCCCTCTCTAAAAGGCTTGATGCAAAATCAGCCCTCAGGGTAAAAGAAGCACAGGAAGGAGACATA
ATGGAAGAAGGAACTGTGTTTATAGCCCCTGGAGATTATCATATGGAAATTGTACGGAATAAAGTAAACGGCTTTGGAGA
AGATACAGTGCATCTATCCTGCGGACCTAAAGAGCTGGGGTCCAGGCCCTCAGTAAATGTCCTGTTCAGATCAGTTGCCA
GGATTTACGGTCCCAGAGTTATTTCCCTTGTACTTACAGGAATGAACTGCGATGGAGCGGATGGAGCTGAAGAGATTAAA
AAAATGGGCGGTAAAGTGATCGCAGAAGACCAAAGCTCATGTGTGATATACGGAATGCCTGGAGAGATTGTAAGGCGAAA
CCTGGCAGACTTTGTGCTTCCTCTGGATAAAATGGCTGATGAAATTGTCAAAATAGTCAGATGA

Upstream 100 bases:

>100_bases
AGATTTACCCTGCCAAATAAAATAACTTTACCAGATAAGATAATTTTGCCAGACAGGATTAATCAGGTCTAACCAGATCT
ACCTTCTAAACCAGATAATT

Downstream 100 bases:

>100_bases
CCCTGGGTAGATCAATTATTAAATTAATTTTTTAGATCAAATCTGGTTAAATTAATTGTTCAGATTGATTGCCAGATTGG
TTACTCAGATTGGTTACTCA

Product: protein-glutamate methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MSNQHPKKVLEMVIRALIVDDSALIRKVLSDILNQDPKIAVIGTAINGEDGLEKVRKLKPDVVLLDNIMPVLDGLKTLSH
IMEEFPTPVVIVSALGEKAEEITLTALEYGAVDVIEKPSGILSQSMHEMAEEICAKVRAVSKADLNNLGCIRDLEHQIPE
NHRKKKNSLREKTSVRNVLAIGASTGGPRALEKLIGSFPAEIPAAVLIVQHMPPGFTASLSKRLDAKSALRVKEAQEGDI
MEEGTVFIAPGDYHMEIVRNKVNGFGEDTVHLSCGPKELGSRPSVNVLFRSVARIYGPRVISLVLTGMNCDGADGAEEIK
KMGGKVIAEDQSSCVIYGMPGEIVRRNLADFVLPLDKMADEIVKIVR

Sequences:

>Translated_367_residues
MSNQHPKKVLEMVIRALIVDDSALIRKVLSDILNQDPKIAVIGTAINGEDGLEKVRKLKPDVVLLDNIMPVLDGLKTLSH
IMEEFPTPVVIVSALGEKAEEITLTALEYGAVDVIEKPSGILSQSMHEMAEEICAKVRAVSKADLNNLGCIRDLEHQIPE
NHRKKKNSLREKTSVRNVLAIGASTGGPRALEKLIGSFPAEIPAAVLIVQHMPPGFTASLSKRLDAKSALRVKEAQEGDI
MEEGTVFIAPGDYHMEIVRNKVNGFGEDTVHLSCGPKELGSRPSVNVLFRSVARIYGPRVISLVLTGMNCDGADGAEEIK
KMGGKVIAEDQSSCVIYGMPGEIVRRNLADFVLPLDKMADEIVKIVR
>Mature_366_residues
SNQHPKKVLEMVIRALIVDDSALIRKVLSDILNQDPKIAVIGTAINGEDGLEKVRKLKPDVVLLDNIMPVLDGLKTLSHI
MEEFPTPVVIVSALGEKAEEITLTALEYGAVDVIEKPSGILSQSMHEMAEEICAKVRAVSKADLNNLGCIRDLEHQIPEN
HRKKKNSLREKTSVRNVLAIGASTGGPRALEKLIGSFPAEIPAAVLIVQHMPPGFTASLSKRLDAKSALRVKEAQEGDIM
EEGTVFIAPGDYHMEIVRNKVNGFGEDTVHLSCGPKELGSRPSVNVLFRSVARIYGPRVISLVLTGMNCDGADGAEEIKK
MGGKVIAEDQSSCVIYGMPGEIVRRNLADFVLPLDKMADEIVKIVR

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=335, Percent_Identity=38.5074626865672, Blast_Score=218, Evalue=5e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB2_METMA (Q8PX96)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_633350.1
- ProteinModelPortal:   Q8PX96
- SMR:   Q8PX96
- GeneID:   1479668
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_1326
- NMPDR:   fig|192952.1.peg.1326
- HOGENOM:   HBG705324
- OMA:   NCDGADG
- ProtClustDB:   CLSK280978
- BioCyc:   MMAZ192952:MM1326-MONOMER
- BRENDA:   3.1.1.61
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 39889; Mature: 39758

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 184-184 ACT_SITE 211-211 ACT_SITE 311-311

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQHPKKVLEMVIRALIVDDSALIRKVLSDILNQDPKIAVIGTAINGEDGLEKVRKLKP
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHCCC
DVVLLDNIMPVLDGLKTLSHIMEEFPTPVVIVSALGEKAEEITLTALEYGAVDVIEKPSG
CEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCHHHHEEEEHHHCCCHHHHCCCCH
ILSQSMHEMAEEICAKVRAVSKADLNNLGCIRDLEHQIPENHRKKKNSLREKTSVRNVLA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHE
IGASTGGPRALEKLIGSFPAEIPAAVLIVQHMPPGFTASLSKRLDAKSALRVKEAQEGDI
ECCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHCCCCCC
MEEGTVFIAPGDYHMEIVRNKVNGFGEDTVHLSCGPKELGSRPSVNVLFRSVARIYGPRV
EECCEEEEECCCHHHHHHHHHHCCCCCCEEEEECCHHHHCCCCCHHHHHHHHHHHHCCCE
ISLVLTGMNCDGADGAEEIKKMGGKVIAEDQSSCVIYGMPGEIVRRNLADFVLPLDKMAD
EHHHHCCCCCCCCCCHHHHHHCCCEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHH
EIVKIVR
HHHHHHC
>Mature Secondary Structure 
SNQHPKKVLEMVIRALIVDDSALIRKVLSDILNQDPKIAVIGTAINGEDGLEKVRKLKP
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHCCC
DVVLLDNIMPVLDGLKTLSHIMEEFPTPVVIVSALGEKAEEITLTALEYGAVDVIEKPSG
CEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCHHHHEEEEHHHCCCHHHHCCCCH
ILSQSMHEMAEEICAKVRAVSKADLNNLGCIRDLEHQIPENHRKKKNSLREKTSVRNVLA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHE
IGASTGGPRALEKLIGSFPAEIPAAVLIVQHMPPGFTASLSKRLDAKSALRVKEAQEGDI
ECCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHCCCCCC
MEEGTVFIAPGDYHMEIVRNKVNGFGEDTVHLSCGPKELGSRPSVNVLFRSVARIYGPRV
EECCEEEEECCCHHHHHHHHHHCCCCCCEEEEECCHHHHCCCCCHHHHHHHHHHHHCCCE
ISLVLTGMNCDGADGAEEIKKMGGKVIAEDQSSCVIYGMPGEIVRRNLADFVLPLDKMAD
EHHHHCCCCCCCCCCHHHHHHCCCEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHH
EIVKIVR
HHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824