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The map label for this gene is leuA2

Identifier: 21227386

GI number: 21227386

Start: 1524905

End: 1526455

Strand: Direct

Name: leuA2

Synonym: MM_1284

Alternate gene names: 21227386

Gene position: 1524905-1526455 (Clockwise)

Preceding gene: 21227385

Following gene: 21227387

Centisome position: 37.23

GC content: 46.36

Gene sequence:

>1551_bases
ATGTACACACTGAAAGAAGGGATCGATTTTTACATCGAACCCATGCAGAGTAAAAAAGTAACTGTTTTTGACACAACACT
GCGTGACGGAGAACAAACCCCTGGAGTATCCCTGACTTCTACACAGAAGCTGGAAATTGCTCATCAACTTGACAAACTCG
GGGTAGACATAATAGAAGCCGGATTTCCCATCTCTTCAGAAGGAGATAAAGAATCCGTAAAATCGATTTCTAATGCCGGG
CTTGACACCACAGTTTGCGGACTGGCTCGTGTGCTGAAAAAAGATATCGATGCCTGTTTTGAAAGTGATGTTGGCCTTGT
GCACACCTTCGTCCCTACCTCGGATGTACAGCGGATCTATACCATCAAAAAGAGCCGGGAAGAAGTCATTCAACTGGCTG
TAGAAGCTGTCCAGTACATCAAAGACCACGGGCTGAAGTGCATGTTTTCCGCAATGGACGCCACCAGAACAGACCCTGAA
TACCTCATAGAGGTTTTCAAAGCGGTTCAGGAAGCAGGCTGCGACATCATCAACGTGCCGGATACTGTTGGCGTGATGGT
CCCGTCTGCAATGTACAGGCAGATAAAAGGCATTGCAGCTGAAATAACGATTCCTATAGACGTGCACTGTCATAATGACT
TCGGGCTTGCTGTGGCAAATAGCCTTATGGCTGTTGAAGCCGGGGCATCTCAGGTACAGGTCACCATAAACGGCATAGGA
GAAAGGGCAGGAAATGCTGATCTTTCCCAGACAGTAATGAGCCTGACATCAATCTACGGTGCAAAAACCAATATCCGGAC
AGAATACCTTGTAGAAACCTCGAAAATGGTTGAAAACTATACAGGAATCAGGCTCCCTCCAAACACGCCTGTGGTGGGGC
AAAACGCATTTTCCCATGAGTCAGGAATTCACAGTCAGGGAGTGCTTGAAAAATCCGACACCTTCGAACCAGGAATTATG
ACTCCGGAAATGGTTGGGCACAGACGGCGCATTGTCCTTGGTAAGCATACCGGCAAACATGCGGTCAAACAGTCCCTTGA
GTCTGCCGGGATAAAAACCAATGATAATCAACTCGATGAAATTGTGATCAGGATTAAAGAAATCGCAAACAAAGGTAAAC
AGATAACAGATGCTGATCTTTATGCAGTTGCCTCTGCAGTTCTCGGTAAAGCGAGTTCTGAGGAAGAGCTGATCAAACTC
AAAGAAGTATCCGTCATGACAGGAAACATCCTTACACCTACGGCTGTGGTTAAGGCAGATATTGAAGGAAAAGAAATCAT
TGCAGCAAGAACAGGAGTCGGTCCTGTGGACGCAGCCCTCCATGCCGTAAGAGACATTCTTGGCGAGAGTAATCATTTCA
GACTCCAGGATTTCAGGATCGATGCTATCACAGGTGGGGCAGATGCCCTTGCCGATGTATACATAGGTCTGGAAAATGAA
AAGGGAAGGATTGTAACAGCCCGGTCCGCAAATCCGGACATAGTAATGGCTTCCGTGGAAGCCCTTGTAAATGCTATGAA
CCTTCTCTATAAAAAAGAAAACAAAAGCTGA

Upstream 100 bases:

>100_bases
CCATTGAAAACTCAAAAAAGTAAAATTTCAGTTAAACATTTAAAAATATACATGATGTTTATATACCAGAACTGTACATC
CATGTAGATGATTACTATGG

Downstream 100 bases:

>100_bases
AAGTAAAGCCCTGAAGAAAAAGTAAAGGATAAGAAAAACTGAAGGGCAAAAAAGGCTGAAAAACCGGAATAATATACCGG
AAACCCGGAAAGAAAAACCG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MYTLKEGIDFYIEPMQSKKVTVFDTTLRDGEQTPGVSLTSTQKLEIAHQLDKLGVDIIEAGFPISSEGDKESVKSISNAG
LDTTVCGLARVLKKDIDACFESDVGLVHTFVPTSDVQRIYTIKKSREEVIQLAVEAVQYIKDHGLKCMFSAMDATRTDPE
YLIEVFKAVQEAGCDIINVPDTVGVMVPSAMYRQIKGIAAEITIPIDVHCHNDFGLAVANSLMAVEAGASQVQVTINGIG
ERAGNADLSQTVMSLTSIYGAKTNIRTEYLVETSKMVENYTGIRLPPNTPVVGQNAFSHESGIHSQGVLEKSDTFEPGIM
TPEMVGHRRRIVLGKHTGKHAVKQSLESAGIKTNDNQLDEIVIRIKEIANKGKQITDADLYAVASAVLGKASSEEELIKL
KEVSVMTGNILTPTAVVKADIEGKEIIAARTGVGPVDAALHAVRDILGESNHFRLQDFRIDAITGGADALADVYIGLENE
KGRIVTARSANPDIVMASVEALVNAMNLLYKKENKS

Sequences:

>Translated_516_residues
MYTLKEGIDFYIEPMQSKKVTVFDTTLRDGEQTPGVSLTSTQKLEIAHQLDKLGVDIIEAGFPISSEGDKESVKSISNAG
LDTTVCGLARVLKKDIDACFESDVGLVHTFVPTSDVQRIYTIKKSREEVIQLAVEAVQYIKDHGLKCMFSAMDATRTDPE
YLIEVFKAVQEAGCDIINVPDTVGVMVPSAMYRQIKGIAAEITIPIDVHCHNDFGLAVANSLMAVEAGASQVQVTINGIG
ERAGNADLSQTVMSLTSIYGAKTNIRTEYLVETSKMVENYTGIRLPPNTPVVGQNAFSHESGIHSQGVLEKSDTFEPGIM
TPEMVGHRRRIVLGKHTGKHAVKQSLESAGIKTNDNQLDEIVIRIKEIANKGKQITDADLYAVASAVLGKASSEEELIKL
KEVSVMTGNILTPTAVVKADIEGKEIIAARTGVGPVDAALHAVRDILGESNHFRLQDFRIDAITGGADALADVYIGLENE
KGRIVTARSANPDIVMASVEALVNAMNLLYKKENKS
>Mature_516_residues
MYTLKEGIDFYIEPMQSKKVTVFDTTLRDGEQTPGVSLTSTQKLEIAHQLDKLGVDIIEAGFPISSEGDKESVKSISNAG
LDTTVCGLARVLKKDIDACFESDVGLVHTFVPTSDVQRIYTIKKSREEVIQLAVEAVQYIKDHGLKCMFSAMDATRTDPE
YLIEVFKAVQEAGCDIINVPDTVGVMVPSAMYRQIKGIAAEITIPIDVHCHNDFGLAVANSLMAVEAGASQVQVTINGIG
ERAGNADLSQTVMSLTSIYGAKTNIRTEYLVETSKMVENYTGIRLPPNTPVVGQNAFSHESGIHSQGVLEKSDTFEPGIM
TPEMVGHRRRIVLGKHTGKHAVKQSLESAGIKTNDNQLDEIVIRIKEIANKGKQITDADLYAVASAVLGKASSEEELIKL
KEVSVMTGNILTPTAVVKADIEGKEIIAARTGVGPVDAALHAVRDILGESNHFRLQDFRIDAITGGADALADVYIGLENE
KGRIVTARSANPDIVMASVEALVNAMNLLYKKENKS

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=511, Percent_Identity=38.3561643835616, Blast_Score=298, Evalue=4e-82,
Organism=Saccharomyces cerevisiae, GI6320071, Length=368, Percent_Identity=30.7065217391304, Blast_Score=192, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6320019, Length=364, Percent_Identity=30.4945054945055, Blast_Score=190, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6324682, Length=311, Percent_Identity=30.2250803858521, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6324225, Length=517, Percent_Identity=26.3056092843327, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24582381, Length=289, Percent_Identity=24.9134948096886, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU12_METMA (P58968)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_633308.1
- ProteinModelPortal:   P58968
- SMR:   P58968
- GeneID:   1479626
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_1284
- NMPDR:   fig|192952.1.peg.1284
- HOGENOM:   HBG701974
- OMA:   ENSFAHE
- ProtClustDB:   PRK00915
- BioCyc:   MMAZ192952:MM1284-MONOMER
- BRENDA:   2.3.3.13
- HAMAP:   MF_01025_A
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011830
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR02090

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 55799; Mature: 55799

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTLKEGIDFYIEPMQSKKVTVFDTTLRDGEQTPGVSLTSTQKLEIAHQLDKLGVDIIEA
CCCHHCCCCEEECCCCCCEEEEEECCCCCCCCCCCCEECCCHHHHHHHHHHHHCHHHHHC
GFPISSEGDKESVKSISNAGLDTTVCGLARVLKKDIDACFESDVGLVHTFVPTSDVQRIY
CCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHH
TIKKSREEVIQLAVEAVQYIKDHGLKCMFSAMDATRTDPEYLIEVFKAVQEAGCDIINVP
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHCCCCCCHHHHHHHHHHHHHCCCCEEECC
DTVGVMVPSAMYRQIKGIAAEITIPIDVHCHNDFGLAVANSLMAVEAGASQVQVTINGIG
CHHHHHCCHHHHHHHCCCEEEEEEEEEEEECCCCCHHHHHHHHEEECCCCEEEEEEEECC
ERAGNADLSQTVMSLTSIYGAKTNIRTEYLVETSKMVENYTGIRLPPNTPVVGQNAFSHE
CCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
SGIHSQGVLEKSDTFEPGIMTPEMVGHRRRIVLGKHTGKHAVKQSLESAGIKTNDNQLDE
CCCCCCCCCCCCCCCCCCCCCHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHH
IVIRIKEIANKGKQITDADLYAVASAVLGKASSEEELIKLKEVSVMTGNILTPTAVVKAD
HHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHEEECCCCCCCCEEEEC
IEGKEIIAARTGVGPVDAALHAVRDILGESNHFRLQDFRIDAITGGADALADVYIGLENE
CCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCHHHHHHEEEEEECC
KGRIVTARSANPDIVMASVEALVNAMNLLYKKENKS
CCCEEEECCCCCCEEHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MYTLKEGIDFYIEPMQSKKVTVFDTTLRDGEQTPGVSLTSTQKLEIAHQLDKLGVDIIEA
CCCHHCCCCEEECCCCCCEEEEEECCCCCCCCCCCCEECCCHHHHHHHHHHHHCHHHHHC
GFPISSEGDKESVKSISNAGLDTTVCGLARVLKKDIDACFESDVGLVHTFVPTSDVQRIY
CCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHH
TIKKSREEVIQLAVEAVQYIKDHGLKCMFSAMDATRTDPEYLIEVFKAVQEAGCDIINVP
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHCCCCCCHHHHHHHHHHHHHCCCCEEECC
DTVGVMVPSAMYRQIKGIAAEITIPIDVHCHNDFGLAVANSLMAVEAGASQVQVTINGIG
CHHHHHCCHHHHHHHCCCEEEEEEEEEEEECCCCCHHHHHHHHEEECCCCEEEEEEEECC
ERAGNADLSQTVMSLTSIYGAKTNIRTEYLVETSKMVENYTGIRLPPNTPVVGQNAFSHE
CCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
SGIHSQGVLEKSDTFEPGIMTPEMVGHRRRIVLGKHTGKHAVKQSLESAGIKTNDNQLDE
CCCCCCCCCCCCCCCCCCCCCHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHH
IVIRIKEIANKGKQITDADLYAVASAVLGKASSEEELIKLKEVSVMTGNILTPTAVVKAD
HHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHEEECCCCCCCCEEEEC
IEGKEIIAARTGVGPVDAALHAVRDILGESNHFRLQDFRIDAITGGADALADVYIGLENE
CCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCHHHHHHEEEEEECC
KGRIVTARSANPDIVMASVEALVNAMNLLYKKENKS
CCCEEEECCCCCCEEHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12125824