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The map label for this gene is rffG [C]

Identifier: 21227264

GI number: 21227264

Start: 1381246

End: 1382250

Strand: Reverse

Name: rffG [C]

Synonym: MM_1162

Alternate gene names: 21227264

Gene position: 1382250-1381246 (Counterclockwise)

Preceding gene: 21227267

Following gene: 21227261

Centisome position: 33.74

GC content: 45.17

Gene sequence:

>1005_bases
ATGTCTAAAATAGTGATCACAGGCGGTGCGGGTTTTATAGGCTCCCATATTGCAGAAAACCTCGCAAAAGATGGTCATGA
GATTGTTATTGTCGATAATCTTGATCCTTACTATTCTGTTGACCTGAAAAAGAAAAATCTGAATATTGCCCTTAACAGCG
GGGATGCCACTTTTATTAATGCTGATGTCACCGACCTTTCAGGAATAAAAGATGTCATTGACAGCACAGTAGATTACGTT
TACCACGAAGCTGCCCAGGCAGGCGTCAGGATTTCGGTAGAGGACCCTTTTAAGCCAAACGATGTAAACGTGCATGGCAC
TCTGAACGTGCTCAAAGCCTCTCTTGACGCGGGCGTAAAAAAGGTCATCAATGCTTCCTCATCTTCCGTCTACGGCAAAG
TAAAATACCTGCCCTTCGACGAACAGCACCCAACAGAGCCAGTATCCCCATACGGCGTCAGTAAATTGGCTGCAGAGCAC
TACTGCAGGGTATTTTACGAAGTATACGGCCTCCCGACCACCTCATTACGCTACTTCACAGTATACGGTCCAAGAATGAG
GCCAGACCTTGCAATCTCAATCTTCACCCGGAAAATGCTTGCAAACGAACCCATCACCGTCTTTGGTGACGGGGAACAGA
CACGGGACTTCACATACATCGAAGACGTCGTGGAAGCTAACAAAAGACTCCTTTACAACAGGGCAACCGACGGTAAAGTC
CTGAACATCGGAGGCGGAAACCGGATCAGTGTAAACAACCTGATAGAAAACTTAAGGTTTATCACCGGCTCAACCTCTGA
AATCATAAACGCCGACAAGCAAAAAGGAGACACAGAAGACACACTTGCAAATGTTGACCTTGGAAATAATATGATAGGAT
ACACACCATTATTCAACATCAATAAAGGCCTGAACAAATTCGTAGACTGGTTTAAAACCGAAAATCAATGCCGTGATCCG
GAACAATCCCTTTTAAGTCAGTCCGCTCCGCAACTTCTAAAGTAA

Upstream 100 bases:

>100_bases
GGAAAAACGGAAGAAAAACGTAACTGAGAATCAACAATTGATGATCCTTCTGAAAAGTTGCCGTAAAAAAACGGTCCAGC
TTCATAACGAGTTGATTTAA

Downstream 100 bases:

>100_bases
TATTCTTAATTTCATCTCCATCTCCAGCTAAAATTAGTCCTCTACTAGTCCTCTTGAGCGAGCAAAGCGAGCGAAAAGGA
CACCGTCCTCCCGAGACGGG

Product: UDP-N-acetylglucosamine 4-epimerase

Products: NA

Alternate protein names: Galactowaldenase; UDP-galactose 4-epimerase [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MSKIVITGGAGFIGSHIAENLAKDGHEIVIVDNLDPYYSVDLKKKNLNIALNSGDATFINADVTDLSGIKDVIDSTVDYV
YHEAAQAGVRISVEDPFKPNDVNVHGTLNVLKASLDAGVKKVINASSSSVYGKVKYLPFDEQHPTEPVSPYGVSKLAAEH
YCRVFYEVYGLPTTSLRYFTVYGPRMRPDLAISIFTRKMLANEPITVFGDGEQTRDFTYIEDVVEANKRLLYNRATDGKV
LNIGGGNRISVNNLIENLRFITGSTSEIINADKQKGDTEDTLANVDLGNNMIGYTPLFNINKGLNKFVDWFKTENQCRDP
EQSLLSQSAPQLLK

Sequences:

>Translated_334_residues
MSKIVITGGAGFIGSHIAENLAKDGHEIVIVDNLDPYYSVDLKKKNLNIALNSGDATFINADVTDLSGIKDVIDSTVDYV
YHEAAQAGVRISVEDPFKPNDVNVHGTLNVLKASLDAGVKKVINASSSSVYGKVKYLPFDEQHPTEPVSPYGVSKLAAEH
YCRVFYEVYGLPTTSLRYFTVYGPRMRPDLAISIFTRKMLANEPITVFGDGEQTRDFTYIEDVVEANKRLLYNRATDGKV
LNIGGGNRISVNNLIENLRFITGSTSEIINADKQKGDTEDTLANVDLGNNMIGYTPLFNINKGLNKFVDWFKTENQCRDP
EQSLLSQSAPQLLK
>Mature_333_residues
SKIVITGGAGFIGSHIAENLAKDGHEIVIVDNLDPYYSVDLKKKNLNIALNSGDATFINADVTDLSGIKDVIDSTVDYVY
HEAAQAGVRISVEDPFKPNDVNVHGTLNVLKASLDAGVKKVINASSSSVYGKVKYLPFDEQHPTEPVSPYGVSKLAAEHY
CRVFYEVYGLPTTSLRYFTVYGPRMRPDLAISIFTRKMLANEPITVFGDGEQTRDFTYIEDVVEANKRLLYNRATDGKVL
NIGGGNRISVNNLIENLRFITGSTSEIINADKQKGDTEDTLANVDLGNNMIGYTPLFNINKGLNKFVDWFKTENQCRDPE
QSLLSQSAPQLLK

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=321, Percent_Identity=33.0218068535826, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI42516563, Length=319, Percent_Identity=30.4075235109718, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI56237023, Length=336, Percent_Identity=25.8928571428571, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI56118217, Length=336, Percent_Identity=25.8928571428571, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI189083684, Length=336, Percent_Identity=25.8928571428571, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI4504031, Length=277, Percent_Identity=29.2418772563177, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI48994969, Length=340, Percent_Identity=29.4117647058824, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1788353, Length=359, Percent_Identity=28.133704735376, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1786974, Length=350, Percent_Identity=26.5714285714286, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788589, Length=275, Percent_Identity=27.2727272727273, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788366, Length=274, Percent_Identity=29.5620437956204, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1790049, Length=318, Percent_Identity=27.9874213836478, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17539532, Length=321, Percent_Identity=30.5295950155763, Blast_Score=134, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17568069, Length=332, Percent_Identity=27.4096385542169, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71982038, Length=351, Percent_Identity=24.5014245014245, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71982035, Length=352, Percent_Identity=24.1477272727273, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI133901790, Length=268, Percent_Identity=29.1044776119403, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17539422, Length=268, Percent_Identity=29.1044776119403, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI133901788, Length=268, Percent_Identity=29.1044776119403, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI133901786, Length=268, Percent_Identity=29.1044776119403, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17539424, Length=268, Percent_Identity=29.1044776119403, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17507723, Length=268, Percent_Identity=28.7313432835821, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI115532424, Length=251, Percent_Identity=25.8964143426295, Blast_Score=68, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=249, Percent_Identity=30.1204819277108, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21356223, Length=319, Percent_Identity=28.2131661442006, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI19923002, Length=337, Percent_Identity=28.486646884273, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24158427, Length=268, Percent_Identity=27.6119402985075, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.2 [H]

Molecular weight: Translated: 36853; Mature: 36722

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIVITGGAGFIGSHIAENLAKDGHEIVIVDNLDPYYSVDLKKKNLNIALNSGDATFIN
CCEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEECCEEEEEECCCEEEEE
ADVTDLSGIKDVIDSTVDYVYHEAAQAGVRISVEDPFKPNDVNVHGTLNVLKASLDAGVK
CCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEHHHHHHHHHHHHHH
KVINASSSSVYGKVKYLPFDEQHPTEPVSPYGVSKLAAEHYCRVFYEVYGLPTTSLRYFT
HHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEE
VYGPRMRPDLAISIFTRKMLANEPITVFGDGEQTRDFTYIEDVVEANKRLLYNRATDGKV
EECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHEEEECCCCCEE
LNIGGGNRISVNNLIENLRFITGSTSEIINADKQKGDTEDTLANVDLGNNMIGYTPLFNI
EECCCCCEEEHHHHHHHHHHHCCCHHHHHCCHHCCCCCHHHEEECCCCCCEECCCCCCCC
NKGLNKFVDWFKTENQCRDPEQSLLSQSAPQLLK
HHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHCC
>Mature Secondary Structure 
SKIVITGGAGFIGSHIAENLAKDGHEIVIVDNLDPYYSVDLKKKNLNIALNSGDATFIN
CEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEECCEEEEEECCCEEEEE
ADVTDLSGIKDVIDSTVDYVYHEAAQAGVRISVEDPFKPNDVNVHGTLNVLKASLDAGVK
CCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEHHHHHHHHHHHHHH
KVINASSSSVYGKVKYLPFDEQHPTEPVSPYGVSKLAAEHYCRVFYEVYGLPTTSLRYFT
HHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEE
VYGPRMRPDLAISIFTRKMLANEPITVFGDGEQTRDFTYIEDVVEANKRLLYNRATDGKV
EECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHEEEECCCCCEE
LNIGGGNRISVNNLIENLRFITGSTSEIINADKQKGDTEDTLANVDLGNNMIGYTPLFNI
EECCCCCEEEHHHHHHHHHHHCCCHHHHHCCHHCCCCCHHHEEECCCCCCEECCCCCCCC
NKGLNKFVDWFKTENQCRDPEQSLLSQSAPQLLK
HHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]