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The map label for this gene is yrbE [H]

Identifier: 21227255

GI number: 21227255

Start: 1370062

End: 1371000

Strand: Direct

Name: yrbE [H]

Synonym: MM_1153

Alternate gene names: 21227255

Gene position: 1370062-1371000 (Clockwise)

Preceding gene: 21227254

Following gene: 21227256

Centisome position: 33.45

GC content: 45.47

Gene sequence:

>939_bases
TTGATAAGAGTAGGAGTAATAGGCACGGGTGCCATGGGCCAGAATCACGTCCGAATCTATAGTGAGATGGAAGGCGTGAA
GCTTGCAGGGATATCCGATGTGGACCAGAGTAGAGTCGAAGCCATGGCTGCCCAGTTTAAAACAAAAGCCTTTACAGATT
ATAAAAAAATGTTTGCCGAAGGCCTTGATGCAGTGAGCGTTGTTGTGCCTACAAAACTGCATAAACAGGTAGTCCTTGAT
GCCCTGGAAGCAGGCCTTCATGTCCTTGTTGAAAAACCCATAGCAGATACGGTTGAAAACGCAGACGTTATGATAGAAGC
TGCAAAAAAGGCAGGTAAAGTGCTCATGGTAGGTCATATCGAACGCTTTAACCCTGCAGTCATAAGGCTTAAGGAGATCA
TAAATTCAGGCACTCTGGGTAAAATAGTATCTATCTCTACAAGAAGAGTAGGCCCGTATAACCCGAGGATAAGAGACGTC
GGCGTAATTCTGGATATCGGTGTCCATGATATAGATATCATTTCATATCTCTACGGCAAGAAAATAAAAGGCGTTTACGC
CATTGCAGGTGCAGACATACACTCTTTTGAAGACCATGCCTCAATTATCCTGCGTATGGACCATAACTTTGCAGGAGTTG
TAGAAACAAACTGGCTGACTCCTCATAAGGTCAGGCACCTGACAGCAATAGGGGTCAAGGGTGTGGCGTATCTGGACTAT
ATTGACCAGACAGTAGAACTGCACGACAACGGCTGGATAAGAAAAGCCAAAGTCGAGCCAAGTGAACCCCTGAAAAATGA
ACTCGCCTATTTCATAGACTGTGTTCAGACAGGTAGAGAACCAAACCCCTGCGGTGAAGATGGGAAACATGCCCTTGAAG
TGGCGATGGCAGCAATCAGGTCTTACGAAGAAGAAAGACTGATTGAAGTGGGGAAGTAA

Upstream 100 bases:

>100_bases
AGACGATGTTCTGAAAGTAATAAAGGAAGTCAAAGAATTTTATGCAAGTGCCTGATCAGTTTTCAGGTTGCAATCACATT
CTTAAAAAGGAAGTGAAATT

Downstream 100 bases:

>100_bases
ACCGCATAAAAGATAAAGGGTGAAGGGAAAAAGAAACCGAAAGCCTCGGGAAAATTCAAAAAAATTTTAAAAAACAATAC
CGGAAAGTGATAAGTATGAG

Product: myo-inositol 2-dehydrogenase

Products: 2,4,6/3,5-pentahydroxycyclohexanone; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MIRVGVIGTGAMGQNHVRIYSEMEGVKLAGISDVDQSRVEAMAAQFKTKAFTDYKKMFAEGLDAVSVVVPTKLHKQVVLD
ALEAGLHVLVEKPIADTVENADVMIEAAKKAGKVLMVGHIERFNPAVIRLKEIINSGTLGKIVSISTRRVGPYNPRIRDV
GVILDIGVHDIDIISYLYGKKIKGVYAIAGADIHSFEDHASIILRMDHNFAGVVETNWLTPHKVRHLTAIGVKGVAYLDY
IDQTVELHDNGWIRKAKVEPSEPLKNELAYFIDCVQTGREPNPCGEDGKHALEVAMAAIRSYEEERLIEVGK

Sequences:

>Translated_312_residues
MIRVGVIGTGAMGQNHVRIYSEMEGVKLAGISDVDQSRVEAMAAQFKTKAFTDYKKMFAEGLDAVSVVVPTKLHKQVVLD
ALEAGLHVLVEKPIADTVENADVMIEAAKKAGKVLMVGHIERFNPAVIRLKEIINSGTLGKIVSISTRRVGPYNPRIRDV
GVILDIGVHDIDIISYLYGKKIKGVYAIAGADIHSFEDHASIILRMDHNFAGVVETNWLTPHKVRHLTAIGVKGVAYLDY
IDQTVELHDNGWIRKAKVEPSEPLKNELAYFIDCVQTGREPNPCGEDGKHALEVAMAAIRSYEEERLIEVGK
>Mature_312_residues
MIRVGVIGTGAMGQNHVRIYSEMEGVKLAGISDVDQSRVEAMAAQFKTKAFTDYKKMFAEGLDAVSVVVPTKLHKQVVLD
ALEAGLHVLVEKPIADTVENADVMIEAAKKAGKVLMVGHIERFNPAVIRLKEIINSGTLGKIVSISTRRVGPYNPRIRDV
GVILDIGVHDIDIISYLYGKKIKGVYAIAGADIHSFEDHASIILRMDHNFAGVVETNWLTPHKVRHLTAIGVKGVAYLDY
IDQTVELHDNGWIRKAKVEPSEPLKNELAYFIDCVQTGREPNPCGEDGKHALEVAMAAIRSYEEERLIEVGK

Specific function: Unknown

COG id: COG0673

COG function: function code R; Predicted dehydrogenases and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Escherichia coli, GI1787574, Length=208, Percent_Identity=32.2115384615385, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87082405, Length=204, Percent_Identity=30.3921568627451, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87081947, Length=193, Percent_Identity=28.4974093264249, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1787307, Length=181, Percent_Identity=27.0718232044199, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789848, Length=133, Percent_Identity=32.3308270676692, Blast_Score=61, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24581117, Length=223, Percent_Identity=25.5605381165919, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: 1.1.1.18

Molecular weight: Translated: 34344; Mature: 34344

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRVGVIGTGAMGQNHVRIYSEMEGVKLAGISDVDQSRVEAMAAQFKTKAFTDYKKMFAE
CEEEEEEECCCCCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GLDAVSVVVPTKLHKQVVLDALEAGLHVLVEKPIADTVENADVMIEAAKKAGKVLMVGHI
CCCEEEEEECHHHHHHHHHHHHHHHHHEEECCCHHHHHCCCHHHEEEHHHCCCEEEEECC
ERFNPAVIRLKEIINSGTLGKIVSISTRRVGPYNPRIRDVGVILDIGVHDIDIISYLYGK
CCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEEEEEEEECCCCHHHHHHHHHCC
KIKGVYAIAGADIHSFEDHASIILRMDHNFAGVVETNWLTPHKVRHLTAIGVKGVAYLDY
CCCCEEEEECCCCCCCCCCEEEEEEECCCEECEEEECCCCHHHHHHHHHHCCCHHHHHHH
IDQTVELHDNGWIRKAKVEPSEPLKNELAYFIDCVQTGREPNPCGEDGKHALEVAMAAIR
HHHHEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
SYEEERLIEVGK
HCCHHHHHCCCC
>Mature Secondary Structure
MIRVGVIGTGAMGQNHVRIYSEMEGVKLAGISDVDQSRVEAMAAQFKTKAFTDYKKMFAE
CEEEEEEECCCCCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GLDAVSVVVPTKLHKQVVLDALEAGLHVLVEKPIADTVENADVMIEAAKKAGKVLMVGHI
CCCEEEEEECHHHHHHHHHHHHHHHHHEEECCCHHHHHCCCHHHEEEHHHCCCEEEEECC
ERFNPAVIRLKEIINSGTLGKIVSISTRRVGPYNPRIRDVGVILDIGVHDIDIISYLYGK
CCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEEEEEEEECCCCHHHHHHHHHCC
KIKGVYAIAGADIHSFEDHASIILRMDHNFAGVVETNWLTPHKVRHLTAIGVKGVAYLDY
CCCCEEEEECCCCCCCCCCEEEEEEECCCEECEEEECCCCHHHHHHHHHHCCCHHHHHHH
IDQTVELHDNGWIRKAKVEPSEPLKNELAYFIDCVQTGREPNPCGEDGKHALEVAMAAIR
HHHHEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
SYEEERLIEVGK
HCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: myo-inositol; NAD+

Specific reaction: myo-inositol + NAD+ = 2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 9025289 [H]