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The map label for this gene is lacA [C]

Identifier: 21227253

GI number: 21227253

Start: 1368204

End: 1368872

Strand: Direct

Name: lacA [C]

Synonym: MM_1151

Alternate gene names: 21227253

Gene position: 1368204-1368872 (Clockwise)

Preceding gene: 21227251

Following gene: 21227254

Centisome position: 33.4

GC content: 41.26

Gene sequence:

>669_bases
ATGAATTCAAAAAAATTCAAAATCCATGATTCTTCCAAGATTTACGGAAATTCTGTAATCGGAAAAGATACTGTGGTTCT
GGAAAATGTAATACTGGGGTATCCGGAACACAAAATCCTCATGGAAATCCTCAAACAAAATATAAAGATTGAGGACTTTG
ATTTTCCGGGCTGCACTATAGGCGCAAACTCGATAATTAGAGCAGGTTCGACAATATTCTCCAGTGTAAAGACCGGGAAT
AACTTCAAGACCGGACATAATGTAATGATCAGGGAAAATACACAAATAGGAGACAACGTACTTATCGGAACGAACGTTAT
CATAGACGGAAACGTAAAGATAGGTAATAACGTCAGCATTCAGGGGAATGTGTATATTCCAACGAATGTGCTTATCGAAG
ACAATGTATTTATTGGACCTTGTGCTGTTCTTGCCAACGACAAATATCCTATCCGCAAAAAGTACGAGCTTAAAGGCCCT
GTTTTGAGAAGAGGAGCATCAATAGGCGCGAACGCGACTTTACTTCCGGGAGTAGAGATTGGAGAAGGAGCAATGGTTGC
CGGAGGAGCCCTTGTAACAAAAGACGTGCCCCCATGGAAACTGGCTCTGGGTGTGCCTGCTAAAATACAGGAACTCCCGG
AAGATCTCAAAGAATTAAACAGGATATAA

Upstream 100 bases:

>100_bases
AAATAAAATATATACTTTCTAATTCATTAAAAATTTTAAGTATGTCTTTGTTCAATTTGGAATTTATCGAACGGATATAA
AGTATACGAAGGGGAAAGAT

Downstream 100 bases:

>100_bases
GCCTACTGAAAAAGCTAATCGAAATAAAAGGAACATAATTAAGATTATAAGAGGTCTAAAAATGATCCCAATCGCCAGGC
CCCTGCTGGGCAAGGAAGAG

Product: acetyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MNSKKFKIHDSSKIYGNSVIGKDTVVLENVILGYPEHKILMEILKQNIKIEDFDFPGCTIGANSIIRAGSTIFSSVKTGN
NFKTGHNVMIRENTQIGDNVLIGTNVIIDGNVKIGNNVSIQGNVYIPTNVLIEDNVFIGPCAVLANDKYPIRKKYELKGP
VLRRGASIGANATLLPGVEIGEGAMVAGGALVTKDVPPWKLALGVPAKIQELPEDLKELNRI

Sequences:

>Translated_222_residues
MNSKKFKIHDSSKIYGNSVIGKDTVVLENVILGYPEHKILMEILKQNIKIEDFDFPGCTIGANSIIRAGSTIFSSVKTGN
NFKTGHNVMIRENTQIGDNVLIGTNVIIDGNVKIGNNVSIQGNVYIPTNVLIEDNVFIGPCAVLANDKYPIRKKYELKGP
VLRRGASIGANATLLPGVEIGEGAMVAGGALVTKDVPPWKLALGVPAKIQELPEDLKELNRI
>Mature_222_residues
MNSKKFKIHDSSKIYGNSVIGKDTVVLENVILGYPEHKILMEILKQNIKIEDFDFPGCTIGANSIIRAGSTIFSSVKTGN
NFKTGHNVMIRENTQIGDNVLIGTNVIIDGNVKIGNNVSIQGNVYIPTNVLIEDNVFIGPCAVLANDKYPIRKKYELKGP
VLRRGASIGANATLLPGVEIGEGAMVAGGALVTKDVPPWKLALGVPAKIQELPEDLKELNRI

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 23990; Mature: 23990

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSKKFKIHDSSKIYGNSVIGKDTVVLENVILGYPEHKILMEILKQNIKIEDFDFPGCTI
CCCCEEEEECCCEEECCCCCCCCCEEEEHHHHCCCCHHHHHHHHHHCCCEEECCCCCCEE
GANSIIRAGSTIFSSVKTGNNFKTGHNVMIRENTQIGDNVLIGTNVIIDGNVKIGNNVSI
CCCHHHHCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEEEECEEEEECCEEECCEEEE
QGNVYIPTNVLIEDNVFIGPCAVLANDKYPIRKKYELKGPVLRRGASIGANATLLPGVEI
EEEEEECCEEEEECCEEECCEEEEECCCCCCCEEEECCCHHHHCCCCCCCCEEEECCCEE
GEGAMVAGGALVTKDVPPWKLALGVPAKIQELPEDLKELNRI
CCCEEEECCEEEECCCCCEEEEECCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNSKKFKIHDSSKIYGNSVIGKDTVVLENVILGYPEHKILMEILKQNIKIEDFDFPGCTI
CCCCEEEEECCCEEECCCCCCCCCEEEEHHHHCCCCHHHHHHHHHHCCCEEECCCCCCEE
GANSIIRAGSTIFSSVKTGNNFKTGHNVMIRENTQIGDNVLIGTNVIIDGNVKIGNNVSI
CCCHHHHCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEEEECEEEEECCEEECCEEEE
QGNVYIPTNVLIEDNVFIGPCAVLANDKYPIRKKYELKGPVLRRGASIGANATLLPGVEI
EEEEEECCEEEEECCEEECCEEEEECCCCCCCEEEECCCHHHHCCCCCCCCEEEECCCEE
GEGAMVAGGALVTKDVPPWKLALGVPAKIQELPEDLKELNRI
CCCEEEECCEEEECCCCCEEEEECCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]