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The map label for this gene is tgtA [H]

Identifier: 21227203

GI number: 21227203

Start: 1294441

End: 1295913

Strand: Direct

Name: tgtA [H]

Synonym: MM_1101

Alternate gene names: 21227203

Gene position: 1294441-1295913 (Clockwise)

Preceding gene: 21227202

Following gene: 21227204

Centisome position: 31.6

GC content: 47.11

Gene sequence:

>1473_bases
ATGTCAGCAATATTTGAAATTCTCGATAAGGACGCAGGCGGCAGGATAGGAAAACTCAGGACTCCTCACGGAGTGGTTGA
GACCCCTACAGTCATGCCTGTTATCAATCCGAACATCCAGCTTATTTCCCCGAAAGAAATGAGAAGTTTCGGGGCAGAGA
TTCTGATCACCAATTCCTACATTATTTACAGGAAAGAAGAACTGCGTACTGTCGCCCTTGAAAAAGGGTTACACGAACTC
CTGGGTTTTGACGGCCCGATTATGACAGACTCCGGCTCTTTCCAGCTTTCAGTATATGGATCTGTGGAAGTTACAAATGA
AGAGATCCTCGGGTTCCAGGAAAAAATAGGAAGCGACATTATAGTCCCACTTGATATCCCGACCCCTCCGGATGTCCATT
TTAGAAGGGCGGAAGAAGAACTTGCGACCACAGCCGAGCGCCTTGAAGCTGCCCGCAAATTCATCCAGAGCAAACAGCTC
CTCGCAGGTCCTGTCCAGGGTTCAACATATCCGGAACTCAGGGAAAAGGCTGCATCCCACCTTAAAGATTTGAATTTTGA
AGTTTATCCCCTGGGAGCAGTTGTGCCTCTCATGGAATCCTACCGTTATGCAGAGCTTGTTGATGTAATTGCAGCATCCA
AAAAAGGACTTTCTCCAACCTCACCTGTTCATCTTTTCGGGGCAGGGCACCCGATGATGTTTGCCCTTGCAGTTGCCCTT
GGCTGCGACCTTTTTGATTCGGCAGCTTATGCCCTTTACGCAAAAGATGGTCGTTATATTACCTCAAACGGGACATACCA
CCTGGAGAAACTGAACTACCTGCCCTGTTCCTGTCCTGTTTGCTCCAGATATACTGCCGAAGAATTAAGAAAAGCTAAAA
ATAAAGAAGAACTTCTCGGGCGGCATAACCTTTATGCGACTTTTGCGGAAATCCGGCTTATTAAACAGTCCATTAAGGAC
GGAAAACTGCTTGAGCTTGTTGAGCAGCGCTGCCGTGCCCACCCGAAACTCCTTGACGGTCTGAAGAGGCTCTATACTCA
CTCTGCATGGCTGGAACAGTTTGACCCTGCAACTAAAGGCACTTATTTCTACTGTGGGCCGGAGTCTGCTTCCAGACCTG
AGGTTCTACGTTTCGGGAAGCGTCTGGAAAGGTTTAGCATTGAAGGTTCGGCGATCATACGCACATCTCCGGTTAAAGGA
GAAAAGGACTATGACCGGATTCTGACATTTAAAGCTCCATTCGGGACCTTCCCGGCAGAAATGGAAGAAGTGTATCCCTT
CAATGCCGAGGTTCCAAAATTTCCGGACTACGAAGCTCTCAGCACGTCCCTGTCAAATACAATTAAACTGATGGATCTGA
ATCCCGGGGCAGAATTTACCTTTATCTGCGAAAAAGAGTTCCAGCATCCTTTAATTGAAGAAATCGGAAAAAAGGCAAAG
CTTGTATACAGGGAAGCCTGGAAAAAAGAATAA

Upstream 100 bases:

>100_bases
AAAAAAGGAGATAAGGAAGACAGATGCAGCTTTTTATCTGTCCCGAACTCCTTCCAATCCCCAAAGATAAATATATCTGA
GCGGCATCTATAACCGCGGC

Downstream 100 bases:

>100_bases
GCAGGCTTAAAGACTGCAACTTGAATAGAACAGACTTCTATTAAGTTTTTGAGAATTGAAGGGCGGAATATCGGTTAGGT
TTAGAACCCTGCTCAATAAC

Product: 7-cyano-7-deazaguanine tRNA-ribosyltransferase

Products: tRNAqueuine; guanine [C]

Alternate protein names: Archaeal tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MSAIFEILDKDAGGRIGKLRTPHGVVETPTVMPVINPNIQLISPKEMRSFGAEILITNSYIIYRKEELRTVALEKGLHEL
LGFDGPIMTDSGSFQLSVYGSVEVTNEEILGFQEKIGSDIIVPLDIPTPPDVHFRRAEEELATTAERLEAARKFIQSKQL
LAGPVQGSTYPELREKAASHLKDLNFEVYPLGAVVPLMESYRYAELVDVIAASKKGLSPTSPVHLFGAGHPMMFALAVAL
GCDLFDSAAYALYAKDGRYITSNGTYHLEKLNYLPCSCPVCSRYTAEELRKAKNKEELLGRHNLYATFAEIRLIKQSIKD
GKLLELVEQRCRAHPKLLDGLKRLYTHSAWLEQFDPATKGTYFYCGPESASRPEVLRFGKRLERFSIEGSAIIRTSPVKG
EKDYDRILTFKAPFGTFPAEMEEVYPFNAEVPKFPDYEALSTSLSNTIKLMDLNPGAEFTFICEKEFQHPLIEEIGKKAK
LVYREAWKKE

Sequences:

>Translated_490_residues
MSAIFEILDKDAGGRIGKLRTPHGVVETPTVMPVINPNIQLISPKEMRSFGAEILITNSYIIYRKEELRTVALEKGLHEL
LGFDGPIMTDSGSFQLSVYGSVEVTNEEILGFQEKIGSDIIVPLDIPTPPDVHFRRAEEELATTAERLEAARKFIQSKQL
LAGPVQGSTYPELREKAASHLKDLNFEVYPLGAVVPLMESYRYAELVDVIAASKKGLSPTSPVHLFGAGHPMMFALAVAL
GCDLFDSAAYALYAKDGRYITSNGTYHLEKLNYLPCSCPVCSRYTAEELRKAKNKEELLGRHNLYATFAEIRLIKQSIKD
GKLLELVEQRCRAHPKLLDGLKRLYTHSAWLEQFDPATKGTYFYCGPESASRPEVLRFGKRLERFSIEGSAIIRTSPVKG
EKDYDRILTFKAPFGTFPAEMEEVYPFNAEVPKFPDYEALSTSLSNTIKLMDLNPGAEFTFICEKEFQHPLIEEIGKKAK
LVYREAWKKE
>Mature_489_residues
SAIFEILDKDAGGRIGKLRTPHGVVETPTVMPVINPNIQLISPKEMRSFGAEILITNSYIIYRKEELRTVALEKGLHELL
GFDGPIMTDSGSFQLSVYGSVEVTNEEILGFQEKIGSDIIVPLDIPTPPDVHFRRAEEELATTAERLEAARKFIQSKQLL
AGPVQGSTYPELREKAASHLKDLNFEVYPLGAVVPLMESYRYAELVDVIAASKKGLSPTSPVHLFGAGHPMMFALAVALG
CDLFDSAAYALYAKDGRYITSNGTYHLEKLNYLPCSCPVCSRYTAEELRKAKNKEELLGRHNLYATFAEIRLIKQSIKDG
KLLELVEQRCRAHPKLLDGLKRLYTHSAWLEQFDPATKGTYFYCGPESASRPEVLRFGKRLERFSIEGSAIIRTSPVKGE
KDYDRILTFKAPFGTFPAEMEEVYPFNAEVPKFPDYEALSTSLSNTIKLMDLNPGAEFTFICEKEFQHPLIEEIGKKAKL
VYREAWKKE

Specific function: Exchanges the guanine residue with 7-cyano-7- deazaguanine (preQ0) at position 15 in the dihydrouridine loop (D- loop) of archaeal tRNAs [H]

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the archaeosine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=349, Percent_Identity=27.2206303724928, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1786607, Length=363, Percent_Identity=27.5482093663912, Blast_Score=128, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI17544680, Length=364, Percent_Identity=24.4505494505494, Blast_Score=104, Evalue=8e-23,
Organism=Drosophila melanogaster, GI19920510, Length=351, Percent_Identity=26.2108262108262, Blast_Score=99, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004804
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: 2.4.2.29 [C]

Molecular weight: Translated: 55033; Mature: 54902

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIFEILDKDAGGRIGKLRTPHGVVETPTVMPVINPNIQLISPKEMRSFGAEILITNSY
CCHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEECHHHHHHCCCEEEEECCE
IIYRKEELRTVALEKGLHELLGFDGPIMTDSGSFQLSVYGSVEVTNEEILGFQEKIGSDI
EEEEHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEEEEEECHHHHHHHHHHHCCCE
IVPLDIPTPPDVHFRRAEEELATTAERLEAARKFIQSKQLLAGPVQGSTYPELREKAASH
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
LKDLNFEVYPLGAVVPLMESYRYAELVDVIAASKKGLSPTSPVHLFGAGHPMMFALAVAL
HHHCCEEEEECHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHH
GCDLFDSAAYALYAKDGRYITSNGTYHLEKLNYLPCSCPVCSRYTAEELRKAKNKEELLG
CCHHHHCCEEEEEECCCEEEECCCCEEEEECCCCCCCCCHHCCCCHHHHHHHCCHHHHHC
RHNLYATFAEIRLIKQSIKDGKLLELVEQRCRAHPKLLDGLKRLYTHSAWLEQFDPATKG
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
TYFYCGPESASRPEVLRFGKRLERFSIEGSAIIRTSPVKGEKDYDRILTFKAPFGTFPAE
CEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCHH
MEEVYPFNAEVPKFPDYEALSTSLSNTIKLMDLNPGAEFTFICEKEFQHPLIEEIGKKAK
HHHHCCCCCCCCCCCCHHHHHHHHHCEEEEEEECCCCCEEEEECHHCCCHHHHHHCHHHH
LVYREAWKKE
HHHHHHHCCC
>Mature Secondary Structure 
SAIFEILDKDAGGRIGKLRTPHGVVETPTVMPVINPNIQLISPKEMRSFGAEILITNSY
CHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEECHHHHHHCCCEEEEECCE
IIYRKEELRTVALEKGLHELLGFDGPIMTDSGSFQLSVYGSVEVTNEEILGFQEKIGSDI
EEEEHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEEEEEECHHHHHHHHHHHCCCE
IVPLDIPTPPDVHFRRAEEELATTAERLEAARKFIQSKQLLAGPVQGSTYPELREKAASH
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
LKDLNFEVYPLGAVVPLMESYRYAELVDVIAASKKGLSPTSPVHLFGAGHPMMFALAVAL
HHHCCEEEEECHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHH
GCDLFDSAAYALYAKDGRYITSNGTYHLEKLNYLPCSCPVCSRYTAEELRKAKNKEELLG
CCHHHHCCEEEEEECCCEEEECCCCEEEEECCCCCCCCCHHCCCCHHHHHHHCCHHHHHC
RHNLYATFAEIRLIKQSIKDGKLLELVEQRCRAHPKLLDGLKRLYTHSAWLEQFDPATKG
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
TYFYCGPESASRPEVLRFGKRLERFSIEGSAIIRTSPVKGEKDYDRILTFKAPFGTFPAE
CEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCHH
MEEVYPFNAEVPKFPDYEALSTSLSNTIKLMDLNPGAEFTFICEKEFQHPLIEEIGKKAK
HHHHCCCCCCCCCCCCHHHHHHHHHCEEEEEEECCCCCEEEEECHHCCCHHHHHHCHHHH
LVYREAWKKE
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Zn; Mg [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.000014 {7-(aminomethyl)-7-deazaguanine}} 0.00006 {guanine}} 0.000053 {guanine}} [C]

Substrates: tRNAguanine; queuine [C]

Specific reaction: tRNAguanine + queuine = tRNAqueuine + guanine [C]

General reaction: Pentosyl group transfer [C]

Inhibitor: Bisimidoester; Dimethyl suberimidate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]