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The map label for this gene is mtaC [H]

Identifier: 21227175

GI number: 21227175

Start: 1262243

End: 1263019

Strand: Direct

Name: mtaC [H]

Synonym: MM_1073

Alternate gene names: 21227175

Gene position: 1262243-1263019 (Clockwise)

Preceding gene: 21227174

Following gene: 21227176

Centisome position: 30.81

GC content: 46.72

Gene sequence:

>777_bases
ATGTTGGATTTGAAACTGGAAGATGTCGATGGCATATTAGTACGCTACAACGTCGCCCTCGAAAAGGAAATGACACCTGA
CGAAGCAGCAGAAGAGCTTTACCCCAAGGATAAACTCATTTATCCAATTGCAAAAGCTATCTACGAGGGAGAAGAAGACG
ATGTTGTAGATGGTCTTCAGGCAGCAATCGATGCAGGAAAGAACCCGATTGCCCTTATTGACGATGCCCTTATGGTAGGA
ATGGGAGTTGTAACCCAGCTTTATGATGATGGTATCATTTTCCTCCCCAACGTTATGATGTCTGCTGACGCTATGCTTGC
AGGTATCGAATATTGTAAGACCAAGACCACAGAAGTTCCCGAGCCAAAGGGCAAGGTTGTCTGCCATGTCGCAGAAGGTG
ACGTCCATGACATCGGAAAGAACATTGTTGTTGCCCTCCTGAGAGCAAACGGCTATGATGTAGTTGACCTCGGAAGAGAT
GTTCCTGTAGATGAGGTCATTGAATGTGTTACAAAAGAGAAGCCGATACTGCTTACAGGTACTGCCCTCATGACAACCAC
CATGTATGCATTCAAGGAAATCAATGACAAACTCCTTGAGAAGGGAATCAAACTGCCCTTTGCATGCGGTGGCGGTGCTG
TGAACCAGGACTTCGTGTCCCAGTATGACCTCGGAGTTTATGGTGAAGAAGCTGCTGATGCCCCCAAGATTGCTGACGCA
ATCGTAGCAGGTACCACAGATATCGCAGCATTAAGAGAGGAATTCCACAAGCACTAA

Upstream 100 bases:

>100_bases
TTTCGGGATGAAATACCTGAAAATTCAGGAAGATTCAACTTGCAGGATCAAAGATCTGAGCAATCTGAGATCTCTTCATT
ATTAAAATGGAGGTTAAAAT

Downstream 100 bases:

>100_bases
GGAGGGAGCAAAATGGTAAAGAAATACACTTCAATGGCTTACGCTAACGCAGATGAACTTATTTTCGGGCAATGTAAATT
CCCGGTAAAGGCAGGCCTTG

Product: methanol corrinoid protein

Products: L-Methionine; 5,6,7,8-Tetrahydrofolate [C]

Alternate protein names: TCP 2 [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLDLKLEDVDGILVRYNVALEKEMTPDEAAEELYPKDKLIYPIAKAIYEGEEDDVVDGLQAAIDAGKNPIALIDDALMVG
MGVVTQLYDDGIIFLPNVMMSADAMLAGIEYCKTKTTEVPEPKGKVVCHVAEGDVHDIGKNIVVALLRANGYDVVDLGRD
VPVDEVIECVTKEKPILLTGTALMTTTMYAFKEINDKLLEKGIKLPFACGGGAVNQDFVSQYDLGVYGEEAADAPKIADA
IVAGTTDIAALREEFHKH

Sequences:

>Translated_258_residues
MLDLKLEDVDGILVRYNVALEKEMTPDEAAEELYPKDKLIYPIAKAIYEGEEDDVVDGLQAAIDAGKNPIALIDDALMVG
MGVVTQLYDDGIIFLPNVMMSADAMLAGIEYCKTKTTEVPEPKGKVVCHVAEGDVHDIGKNIVVALLRANGYDVVDLGRD
VPVDEVIECVTKEKPILLTGTALMTTTMYAFKEINDKLLEKGIKLPFACGGGAVNQDFVSQYDLGVYGEEAADAPKIADA
IVAGTTDIAALREEFHKH
>Mature_258_residues
MLDLKLEDVDGILVRYNVALEKEMTPDEAAEELYPKDKLIYPIAKAIYEGEEDDVVDGLQAAIDAGKNPIALIDDALMVG
MGVVTQLYDDGIIFLPNVMMSADAMLAGIEYCKTKTTEVPEPKGKVVCHVAEGDVHDIGKNIVVALLRANGYDVVDLGRD
VPVDEVIECVTKEKPILLTGTALMTTTMYAFKEINDKLLEKGIKLPFACGGGAVNQDFVSQYDLGVYGEEAADAPKIADA
IVAGTTDIAALREEFHKH

Specific function: Acts probably as a methyl group carrier between mttB and either mtbA or mtaA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=197, Percent_Identity=28.9340101522843, Blast_Score=78, Evalue=9e-15,
Organism=Escherichia coli, GI1790450, Length=182, Percent_Identity=32.4175824175824, Blast_Score=97, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17535405, Length=183, Percent_Identity=33.3333333333333, Blast_Score=81, Evalue=5e-16,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR003759
- InterPro:   IPR012741 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2 [H]

EC number: 2.1.1.13 [C]

Molecular weight: Translated: 27920; Mature: 27920

Theoretical pI: Translated: 4.11; Mature: 4.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLKLEDVDGILVRYNVALEKEMTPDEAAEELYPKDKLIYPIAKAIYEGEEDDVVDGLQ
CCCCEEECCCCEEEEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHH
AAIDAGKNPIALIDDALMVGMGVVTQLYDDGIIFLPNVMMSADAMLAGIEYCKTKTTEVP
HHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCC
EPKGKVVCHVAEGDVHDIGKNIVVALLRANGYDVVDLGRDVPVDEVIECVTKEKPILLTG
CCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHCCCCCEEEEC
TALMTTTMYAFKEINDKLLEKGIKLPFACGGGAVNQDFVSQYDLGVYGEEAADAPKIADA
HHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHH
IVAGTTDIAALREEFHKH
HHCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MLDLKLEDVDGILVRYNVALEKEMTPDEAAEELYPKDKLIYPIAKAIYEGEEDDVVDGLQ
CCCCEEECCCCEEEEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHH
AAIDAGKNPIALIDDALMVGMGVVTQLYDDGIIFLPNVMMSADAMLAGIEYCKTKTTEVP
HHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCC
EPKGKVVCHVAEGDVHDIGKNIVVALLRANGYDVVDLGRDVPVDEVIECVTKEKPILLTG
CCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHCCCCCEEEEC
TALMTTTMYAFKEINDKLLEKGIKLPFACGGGAVNQDFVSQYDLGVYGEEAADAPKIADA
HHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHH
IVAGTTDIAALREEFHKH
HHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP; Cobinamide; S-adenosyl-L-methionine [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 500-780 [C]

Specific activity: 3.8

Km value (mM): 0.035 {S-methyltetrahydrofolate}} 0.0016 {S-adenosyl-L-methionine}} 0.00065 {S-adenosyl-L-methionine}} 0.06 {methyltetrahydrofolate}} 0.03 {methyltetrahydrofolate}} 0.016 {homocysteine}} [C]

Substrates: 5-Methyltetrahydrofolate; L-Homocysteine [C]

Specific reaction: 5-Methyltetrahydrofolate + L-Homocysteine --> L-Methionine + 5,6,7,8-Tetrahydrofolate [C]

General reaction: Methyl group transfer [C]

Inhibitor: 5, 6-Dimethyl benzimidazole; alpha-Ribazole 3'-phosphate; Be2+; Cobinamide; Propyl iodide; Sr2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]