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The map label for this gene is ddlB [C]

Identifier: 21227150

GI number: 21227150

Start: 1225914

End: 1228016

Strand: Reverse

Name: ddlB [C]

Synonym: MM_1048

Alternate gene names: 21227150

Gene position: 1228016-1225914 (Counterclockwise)

Preceding gene: 21227151

Following gene: 21227137

Centisome position: 29.98

GC content: 47.74

Gene sequence:

>2103_bases
ATGACCCAGAATAATATTGATTCTAATTCCAGGCGCAAACGCGCTAAAGGTGCCGTACAGACGAAAACCCTCGGGCCTGT
TGAAGACCTTGAAACTCATGTCCGGTCCGACTGGTGGCAGACTCTTTTTAATTCTCTCTATCTCAAGACTGATGCCGATC
TTCTGGATGACATTGAAGTTACAAAGAAAGAAGCTGATCTTGTTGTTTCTATCCTTGGGCTCAACCCTGAAGATGCTGTT
CTTGACCTTTGCTGCGGACAGGGCAGGCATGTCCTGGAACTGGCAAGGAGAGGATTTCCAAATGTTGAAGGCTATGACAG
ATCCCAGTACCTCATCCGAAAAGCAAGGACAAGGGCTCAAAAGGAAAACCTGCAGGTCAGGTTCAGGGAGGGGGACGCAA
GAAAGCTGCCTTATCCATCTGACACCTTTTCTGTGGTTACCATTCTCGGGAACAGCTTTGGATACTTTGATTCCACTCTG
CAGGACAGAAAAGTGCTCGAAGAAGTTTTCCGGGTACTGAAGCCAGGTGGGAGAGTCTTTATTGACGCTGTAGACGGGGA
CCACATTAGAAAGAATTTCCAGCCAAGATCATGGGAATGGCTGGACAGGAAGTATTTTGTATGTAGGGAAAGAGCCCTTT
CTTCAGACTGTGAAAGGTTGATCTGCAGGGAGGTTATATGCCGTATTGATAGAGGGATAATTGCAGACCAGTTTTACGCT
GAAAGGCTGTACAACAGGGAAAGCCTTTTTGAACTGCTTACGGCATCAGGTTTTAGCAGCCCGACCTTCCACACAACCTT
CAGCCCTGTCTCGACAGGCACTCAGGACGCAGGAATGATGGAAAAGAGAATTCTGCTTTCAGCCACTGTTGAGAAAGCCT
GGCCTTCCATGGAGTCTTCCGGTGCGGAAAGCAAAAAGCCCCTTAACGTTGTTGTGGTGCTTGGGGATCCAAGGAAAGAA
GACCAGGTCAAGCCTGCCTGTGTATTTGACGATGATGATTTCGAAACCATTAACCGGATGAAGACGGCTCTTTCTGAAAT
TCCTTCTATAAAATTCACTTTCCTTGACAGACATGAAACCCTTTTCGAAGACCTCAGAAAGAAAGCAGGGAAAACCGACC
TTGTACTCAACCTCTGCGATGAAGGTTTTTACAACGATCCTGTAAAAGAATTACACGTTCCTGCCATGTTTGAACAGCTT
AATATCCCTTACACCGGCGCAGGTCCACAGTGCCTTGCCTACTGCTATGATAAATCTCTTGTAAGGGGAGTAGCTCGTGA
GATGAGGATTCCTGTGGCAAAAGGGGTTCTCGTTACAGATGATTCTGATATCTCAAGGCTTTCTCTCTCTTTTCCCCTGC
TCGTAAAACCCAATTCAGGAGATTCAAGTTTCGGGATCACACAGAAAAGCATAGTCCACAGCAGAGAGGAACTTCTTGAC
ATCATGAAAGAAACACGGGAAAAAATAGGGTCAGACAAGCCTCTCCTTCTGGAGGAATTCCTCCCTGGAAAAGACATCAG
TGTAGGTATAATCGGAAACCCTCCTTCGTGCACCGTGCTGCCCATTACGGAAGAAGATTATTCCGCAGTCCCGGGAGAAC
TGCCAAAGATTTGCGGGTATGAAGCCAAATGGCTCCCGGATTCTTCTTACTGGAAGATTAAATCCGTACCTGCGGACCTT
CCTGAAAAGACCCAGAAGGAAGTCGTAAGGAGCTGCCTTGCTCTCTTTACAAGGCTGGGATGCCGTGATTATGCCCGCTT
TGATTGGAGGCTCGACGCTGAAGGCAGGCCAAGGCTCCTTGAGGTAAACCCCAACCCTGGATGGTGCTGGGACGGTCACC
TTGCCAAAATGGCAGCTTATGCGAACATCTCTTATTCCGGTATGCTTGCGGCAATTATTGGGGCTGCAAAGAAAAGATAT
GGTATCGGGCTGGGCGGCAAGGTTGTGCTTCAAACGAAGGCAGGCCAGTCCACAAGAAAAAGCCCGGCTGAATGTGCTGC
GGAATTCGAGGAAGAAGTTGAGGCTAAAAGCTCAATTGAGCCCGGAAATGGCAGGAAGAGAAATGTTTTCTCAAGCAATT
CCGAAACTATAAAGGTTCTCTAA

Upstream 100 bases:

>100_bases
CTCTATCCAGCTGACCCTTTGAGTATATAAATCTCCCTAACTGTCCGATAAACTTGATATACTGAAAGCACATACATTTT
TTTCGGGAAGATATCTTATC

Downstream 100 bases:

>100_bases
GAGTAGAGGCTCTCTCATAGTGTATGGTTAAAACAATCAAAAATCGGTTAAAAAAATTTGATTTGGAAATAAACCCGGAT
CAAATAAGTGGGTCTGAAAA

Product: D-alanine-D-alanine ligase-like protein

Products: ADP; D-Alanyl-D-alanine; H+; Phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 700; Mature: 699

Protein sequence:

>700_residues
MTQNNIDSNSRRKRAKGAVQTKTLGPVEDLETHVRSDWWQTLFNSLYLKTDADLLDDIEVTKKEADLVVSILGLNPEDAV
LDLCCGQGRHVLELARRGFPNVEGYDRSQYLIRKARTRAQKENLQVRFREGDARKLPYPSDTFSVVTILGNSFGYFDSTL
QDRKVLEEVFRVLKPGGRVFIDAVDGDHIRKNFQPRSWEWLDRKYFVCRERALSSDCERLICREVICRIDRGIIADQFYA
ERLYNRESLFELLTASGFSSPTFHTTFSPVSTGTQDAGMMEKRILLSATVEKAWPSMESSGAESKKPLNVVVVLGDPRKE
DQVKPACVFDDDDFETINRMKTALSEIPSIKFTFLDRHETLFEDLRKKAGKTDLVLNLCDEGFYNDPVKELHVPAMFEQL
NIPYTGAGPQCLAYCYDKSLVRGVAREMRIPVAKGVLVTDDSDISRLSLSFPLLVKPNSGDSSFGITQKSIVHSREELLD
IMKETREKIGSDKPLLLEEFLPGKDISVGIIGNPPSCTVLPITEEDYSAVPGELPKICGYEAKWLPDSSYWKIKSVPADL
PEKTQKEVVRSCLALFTRLGCRDYARFDWRLDAEGRPRLLEVNPNPGWCWDGHLAKMAAYANISYSGMLAAIIGAAKKRY
GIGLGGKVVLQTKAGQSTRKSPAECAAEFEEEVEAKSSIEPGNGRKRNVFSSNSETIKVL

Sequences:

>Translated_700_residues
MTQNNIDSNSRRKRAKGAVQTKTLGPVEDLETHVRSDWWQTLFNSLYLKTDADLLDDIEVTKKEADLVVSILGLNPEDAV
LDLCCGQGRHVLELARRGFPNVEGYDRSQYLIRKARTRAQKENLQVRFREGDARKLPYPSDTFSVVTILGNSFGYFDSTL
QDRKVLEEVFRVLKPGGRVFIDAVDGDHIRKNFQPRSWEWLDRKYFVCRERALSSDCERLICREVICRIDRGIIADQFYA
ERLYNRESLFELLTASGFSSPTFHTTFSPVSTGTQDAGMMEKRILLSATVEKAWPSMESSGAESKKPLNVVVVLGDPRKE
DQVKPACVFDDDDFETINRMKTALSEIPSIKFTFLDRHETLFEDLRKKAGKTDLVLNLCDEGFYNDPVKELHVPAMFEQL
NIPYTGAGPQCLAYCYDKSLVRGVAREMRIPVAKGVLVTDDSDISRLSLSFPLLVKPNSGDSSFGITQKSIVHSREELLD
IMKETREKIGSDKPLLLEEFLPGKDISVGIIGNPPSCTVLPITEEDYSAVPGELPKICGYEAKWLPDSSYWKIKSVPADL
PEKTQKEVVRSCLALFTRLGCRDYARFDWRLDAEGRPRLLEVNPNPGWCWDGHLAKMAAYANISYSGMLAAIIGAAKKRY
GIGLGGKVVLQTKAGQSTRKSPAECAAEFEEEVEAKSSIEPGNGRKRNVFSSNSETIKVL
>Mature_699_residues
TQNNIDSNSRRKRAKGAVQTKTLGPVEDLETHVRSDWWQTLFNSLYLKTDADLLDDIEVTKKEADLVVSILGLNPEDAVL
DLCCGQGRHVLELARRGFPNVEGYDRSQYLIRKARTRAQKENLQVRFREGDARKLPYPSDTFSVVTILGNSFGYFDSTLQ
DRKVLEEVFRVLKPGGRVFIDAVDGDHIRKNFQPRSWEWLDRKYFVCRERALSSDCERLICREVICRIDRGIIADQFYAE
RLYNRESLFELLTASGFSSPTFHTTFSPVSTGTQDAGMMEKRILLSATVEKAWPSMESSGAESKKPLNVVVVLGDPRKED
QVKPACVFDDDDFETINRMKTALSEIPSIKFTFLDRHETLFEDLRKKAGKTDLVLNLCDEGFYNDPVKELHVPAMFEQLN
IPYTGAGPQCLAYCYDKSLVRGVAREMRIPVAKGVLVTDDSDISRLSLSFPLLVKPNSGDSSFGITQKSIVHSREELLDI
MKETREKIGSDKPLLLEEFLPGKDISVGIIGNPPSCTVLPITEEDYSAVPGELPKICGYEAKWLPDSSYWKIKSVPADLP
EKTQKEVVRSCLALFTRLGCRDYARFDWRLDAEGRPRLLEVNPNPGWCWDGHLAKMAAYANISYSGMLAAIIGAAKKRYG
IGLGGKVVLQTKAGQSTRKSPAECAAEFEEEVEAKSSIEPGNGRKRNVFSSNSETIKVL

Specific function: Could be involved in the biosynthesis of a cell wall component [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=250, Percent_Identity=28, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1786579, Length=256, Percent_Identity=21.484375, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011095
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C [H]

EC number: 6.3.2.4 [C]

Molecular weight: Translated: 78681; Mature: 78550

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQNNIDSNSRRKRAKGAVQTKTLGPVEDLETHVRSDWWQTLFNSLYLKTDADLLDDIEV
CCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHH
TKKEADLVVSILGLNPEDAVLDLCCGQGRHVLELARRGFPNVEGYDRSQYLIRKARTRAQ
HHHHHCEEEEEECCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
KENLQVRFREGDARKLPYPSDTFSVVTILGNSFGYFDSTLQDRKVLEEVFRVLKPGGRVF
HCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEE
IDAVDGDHIRKNFQPRSWEWLDRKYFVCRERALSSDCERLICREVICRIDRGIIADQFYA
EECCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ERLYNRESLFELLTASGFSSPTFHTTFSPVSTGTQDAGMMEKRILLSATVEKAWPSMESS
HHHHCHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
GAESKKPLNVVVVLGDPRKEDQVKPACVFDDDDFETINRMKTALSEIPSIKFTFLDRHET
CCCCCCCCEEEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCEEEEEECHHHH
LFEDLRKKAGKTDLVLNLCDEGFYNDPVKELHVPAMFEQLNIPYTGAGPQCLAYCYDKSL
HHHHHHHHCCCCCEEHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
VRGVAREMRIPVAKGVLVTDDSDISRLSLSFPLLVKPNSGDSSFGITQKSIVHSREELLD
HHHHHHHHCCCHHCCEEEECCCCCCEEEECCEEEEECCCCCCCCCCCHHHHHHHHHHHHH
IMKETREKIGSDKPLLLEEFLPGKDISVGIIGNPPSCTVLPITEEDYSAVPGELPKICGY
HHHHHHHHHCCCCCCHHHHCCCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCCHHHCCC
EAKWLPDSSYWKIKSVPADLPEKTQKEVVRSCLALFTRLGCRDYARFDWRLDAEGRPRLL
CCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCEEEEECCCCCCEEE
EVNPNPGWCWDGHLAKMAAYANISYSGMLAAIIGAAKKRYGIGLGGKVVLQTKAGQSTRK
EECCCCCCEECHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCC
SPAECAAEFEEEVEAKSSIEPGNGRKRNVFSSNSETIKVL
CHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCEEEEC
>Mature Secondary Structure 
TQNNIDSNSRRKRAKGAVQTKTLGPVEDLETHVRSDWWQTLFNSLYLKTDADLLDDIEV
CCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHH
TKKEADLVVSILGLNPEDAVLDLCCGQGRHVLELARRGFPNVEGYDRSQYLIRKARTRAQ
HHHHHCEEEEEECCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
KENLQVRFREGDARKLPYPSDTFSVVTILGNSFGYFDSTLQDRKVLEEVFRVLKPGGRVF
HCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEE
IDAVDGDHIRKNFQPRSWEWLDRKYFVCRERALSSDCERLICREVICRIDRGIIADQFYA
EECCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ERLYNRESLFELLTASGFSSPTFHTTFSPVSTGTQDAGMMEKRILLSATVEKAWPSMESS
HHHHCHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
GAESKKPLNVVVVLGDPRKEDQVKPACVFDDDDFETINRMKTALSEIPSIKFTFLDRHET
CCCCCCCCEEEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCEEEEEECHHHH
LFEDLRKKAGKTDLVLNLCDEGFYNDPVKELHVPAMFEQLNIPYTGAGPQCLAYCYDKSL
HHHHHHHHCCCCCEEHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
VRGVAREMRIPVAKGVLVTDDSDISRLSLSFPLLVKPNSGDSSFGITQKSIVHSREELLD
HHHHHHHHCCCHHCCEEEECCCCCCEEEECCEEEEECCCCCCCCCCCHHHHHHHHHHHHH
IMKETREKIGSDKPLLLEEFLPGKDISVGIIGNPPSCTVLPITEEDYSAVPGELPKICGY
HHHHHHHHHCCCCCCHHHHCCCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCCHHHCCC
EAKWLPDSSYWKIKSVPADLPEKTQKEVVRSCLALFTRLGCRDYARFDWRLDAEGRPRLL
CCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCEEEEECCCCCCEEE
EVNPNPGWCWDGHLAKMAAYANISYSGMLAAIIGAAKKRYGIGLGGKVVLQTKAGQSTRK
EECCCCCCEECHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCC
SPAECAAEFEEEVEAKSSIEPGNGRKRNVFSSNSETIKVL
CHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 1018 [C]

Specific activity: 31

Km value (mM): 15 {D-norleucine}} 6 {D-Phe}} 86 {D-Ala}} 70.3 {D-Ala}} 60.4 {D-Ala}} 57.5 {D-Ala}} 43.7 {D-Ala}} 38 {D-Ala}} 25.7 {D-Ala}} 22.9 {D-Ala}} 22.8 {D-Ala}} 20.9 {D-Ala}} 6.4 {D-Ala}} 5.4 {D-Ala}} 1.2 {D

Substrates: D-Alanine; ATP [C]

Specific reaction: (2) D-Alanine + ATP <==> ADP + D-Alanyl-D-alanine + H+ + Phosphate [C]

General reaction: Carboxamide formation; Carboxylic acid amide formation [C]

Inhibitor: [1 (S)-Aminoethyl] [(2RS) 2-carboxy-1-octyl] phosphinic acid; Aminoalkyl phosphinate; D-Ala-D-2-hydroxy; D-Ala-D-Ala; D-Ala-D-Alaphosphinate; D-Cycloserine; Gly; Gly-Gly; Phosphinates; Phosphonate dipeptide analogs [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]