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The map label for this gene is leuA [H]

Identifier: 21227104

GI number: 21227104

Start: 1175568

End: 1176890

Strand: Direct

Name: leuA [H]

Synonym: MM_1002

Alternate gene names: 21227104

Gene position: 1175568-1176890 (Clockwise)

Preceding gene: 21227094

Following gene: 21227106

Centisome position: 28.7

GC content: 44.97

Gene sequence:

>1323_bases
ATGAAAATATATGAATCATATGAGGACCTCCCCAAGCTGAAGCTGCCCTATGGGAACGAGGTATTCATAAGTGACAGCAC
CATTCGCGACGGTTCGCAGATGCCGGGAATAGTCCTGAGCAGAGAACACAAGATTCAAATTTATGAATACCTCCACGAAA
TAGGGATCGAGAAACTTGAAGCCTTTGTATTCAACAAAAGAGACAGGGACGCTGTAGACCTGATGTTCGATAAGGGATAC
GAATTCCCCGAAATTACAGGATGGGCGAGGTCTTCAAGAGCAGACATAGATAAAATTCTGGAAGTTGACGGAATTGAAGA
AACCGGGATTTTGATGTCGGTCTCGGATACGCACATTCATTCCAAGATGAGACTTTCAGGCAGGGAAGAGGCGGAAGAAA
GATATCTCGATGCCCTTCAATACGCTGTTGACCACGGGCTGAAGACAAGGGCTCACCTTGAGGATATGACAAGAGCTGAT
AACTATGGCTTTGTTTTCCCCCTTGTAAAAAAGATAATGGAAATCGACCCTGACTGTATAATAAGAGTCTGCGATACCGT
TGGGTACGGGATGCCGTTCATGAATATCGATGAGCCATACGGAATCCCGAAAATTATCCAGCACCTTAAAAAAGAAATTG
GGGTCAAAAATATAGAAACCCACATCCATGACGATTACGGGTTCGGTTCCGCAAGTTCGATTGCAGGTTTCTGGCATGGA
GCCAACTGGACAAGTGTGACTTTCCTTGGAATAGGAGAACGTGCAGGAAACAGCGAGATGGAAAAAATCCTTCTCTTCCT
GGCTGACAGGGTTGAAGGTTTTGACAAATATAACCTTGAGCCTGTAACCCGTTTTGCGGAATTTATGGAAAAAGAGCTCG
GACTCAGAGTCCCGAGAAATAAAGCTGTTGTCGGGAAAAATATCTTTGCCCATGAGTCAGGCATCCACGCCGCAGGCGTC
CTGAAAAATCCATTTAACTATGAGCCCTACCCTCCTGAACTGGTCGGGGGCACAAGGCTTCTCCTCATAGGTGATTCATC
GGGCCTCGAAGTAATACGCTATAAGGTTCAGGAAACCTTAAATAACCTCCTTGATGTCGAAACCGTAGTAGAAAAAGACG
ACAGGAGACTGCTTAAGATCCAGAAAGAGATCCAGAAGCTTTACGATAAAGAAGAGAGGGTTTCCTGTATCTCAGATGAA
GAGCTCCTTGCTTACGTTGAAAAGTACTTCCTGTATCAGCCAATCTCTGATCCTGCACGAACAGGCAGAGGAAAACTCAA
AAACAGAGGGAAAATTCAGGAACCTGTAGAAGAAAAAGAATGA

Upstream 100 bases:

>100_bases
TTATACATAACAGCATCTTTTTCGAAGCAAAAATCCAGCAGAGTAAGCCCACTCTCTCGACACAGGGCCATGCATCCTGA
AAAAGATAGGAGACGAATAT

Downstream 100 bases:

>100_bases
AGTCACAAAAAAGGTAAAAAGAAATATCAGATTAAATGGTGTGAGGTTTTTCAGACCTGATATCAACCTTATTTAACCTT
ACACAAATTAATCTCTTATT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MKIYESYEDLPKLKLPYGNEVFISDSTIRDGSQMPGIVLSREHKIQIYEYLHEIGIEKLEAFVFNKRDRDAVDLMFDKGY
EFPEITGWARSSRADIDKILEVDGIEETGILMSVSDTHIHSKMRLSGREEAEERYLDALQYAVDHGLKTRAHLEDMTRAD
NYGFVFPLVKKIMEIDPDCIIRVCDTVGYGMPFMNIDEPYGIPKIIQHLKKEIGVKNIETHIHDDYGFGSASSIAGFWHG
ANWTSVTFLGIGERAGNSEMEKILLFLADRVEGFDKYNLEPVTRFAEFMEKELGLRVPRNKAVVGKNIFAHESGIHAAGV
LKNPFNYEPYPPELVGGTRLLLIGDSSGLEVIRYKVQETLNNLLDVETVVEKDDRRLLKIQKEIQKLYDKEERVSCISDE
ELLAYVEKYFLYQPISDPARTGRGKLKNRGKIQEPVEEKE

Sequences:

>Translated_440_residues
MKIYESYEDLPKLKLPYGNEVFISDSTIRDGSQMPGIVLSREHKIQIYEYLHEIGIEKLEAFVFNKRDRDAVDLMFDKGY
EFPEITGWARSSRADIDKILEVDGIEETGILMSVSDTHIHSKMRLSGREEAEERYLDALQYAVDHGLKTRAHLEDMTRAD
NYGFVFPLVKKIMEIDPDCIIRVCDTVGYGMPFMNIDEPYGIPKIIQHLKKEIGVKNIETHIHDDYGFGSASSIAGFWHG
ANWTSVTFLGIGERAGNSEMEKILLFLADRVEGFDKYNLEPVTRFAEFMEKELGLRVPRNKAVVGKNIFAHESGIHAAGV
LKNPFNYEPYPPELVGGTRLLLIGDSSGLEVIRYKVQETLNNLLDVETVVEKDDRRLLKIQKEIQKLYDKEERVSCISDE
ELLAYVEKYFLYQPISDPARTGRGKLKNRGKIQEPVEEKE
>Mature_440_residues
MKIYESYEDLPKLKLPYGNEVFISDSTIRDGSQMPGIVLSREHKIQIYEYLHEIGIEKLEAFVFNKRDRDAVDLMFDKGY
EFPEITGWARSSRADIDKILEVDGIEETGILMSVSDTHIHSKMRLSGREEAEERYLDALQYAVDHGLKTRAHLEDMTRAD
NYGFVFPLVKKIMEIDPDCIIRVCDTVGYGMPFMNIDEPYGIPKIIQHLKKEIGVKNIETHIHDDYGFGSASSIAGFWHG
ANWTSVTFLGIGERAGNSEMEKILLFLADRVEGFDKYNLEPVTRFAEFMEKELGLRVPRNKAVVGKNIFAHESGIHAAGV
LKNPFNYEPYPPELVGGTRLLLIGDSSGLEVIRYKVQETLNNLLDVETVVEKDDRRLLKIQKEIQKLYDKEERVSCISDE
ELLAYVEKYFLYQPISDPARTGRGKLKNRGKIQEPVEEKE

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=368, Percent_Identity=29.0760869565217, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320019, Length=372, Percent_Identity=25.2688172043011, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320071, Length=354, Percent_Identity=25.4237288135593, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011830
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 50397; Mature: 50397

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYESYEDLPKLKLPYGNEVFISDSTIRDGSQMPGIVLSREHKIQIYEYLHEIGIEKLE
CCCHHCHHCCCCCCCCCCCEEEEECCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHH
AFVFNKRDRDAVDLMFDKGYEFPEITGWARSSRADIDKILEVDGIEETGILMSVSDTHIH
HHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCHHHH
SKMRLSGREEAEERYLDALQYAVDHGLKTRAHLEDMTRADNYGFVFPLVKKIMEIDPDCI
HHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHH
IRVCDTVGYGMPFMNIDEPYGIPKIIQHLKKEIGVKNIETHIHDDYGFGSASSIAGFWHG
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHEEC
ANWTSVTFLGIGERAGNSEMEKILLFLADRVEGFDKYNLEPVTRFAEFMEKELGLRVPRN
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
KAVVGKNIFAHESGIHAAGVLKNPFNYEPYPPELVGGTRLLLIGDSSGLEVIRYKVQETL
CEEECCCHHHCCCCCCCHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHH
NNLLDVETVVEKDDRRLLKIQKEIQKLYDKEERVSCISDEELLAYVEKYFLYQPISDPAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHH
TGRGKLKNRGKIQEPVEEKE
CCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKIYESYEDLPKLKLPYGNEVFISDSTIRDGSQMPGIVLSREHKIQIYEYLHEIGIEKLE
CCCHHCHHCCCCCCCCCCCEEEEECCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHH
AFVFNKRDRDAVDLMFDKGYEFPEITGWARSSRADIDKILEVDGIEETGILMSVSDTHIH
HHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCHHHH
SKMRLSGREEAEERYLDALQYAVDHGLKTRAHLEDMTRADNYGFVFPLVKKIMEIDPDCI
HHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHH
IRVCDTVGYGMPFMNIDEPYGIPKIIQHLKKEIGVKNIETHIHDDYGFGSASSIAGFWHG
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHEEC
ANWTSVTFLGIGERAGNSEMEKILLFLADRVEGFDKYNLEPVTRFAEFMEKELGLRVPRN
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
KAVVGKNIFAHESGIHAAGVLKNPFNYEPYPPELVGGTRLLLIGDSSGLEVIRYKVQETL
CEEECCCHHHCCCCCCCHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHH
NNLLDVETVVEKDDRRLLKIQKEIQKLYDKEERVSCISDEELLAYVEKYFLYQPISDPAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHH
TGRGKLKNRGKIQEPVEEKE
CCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA