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The map label for this gene is cbiHC [H]

Identifier: 21227097

GI number: 21227097

Start: 1170895

End: 1171692

Strand: Reverse

Name: cbiHC [H]

Synonym: MM_0995

Alternate gene names: 21227097

Gene position: 1171692-1170895 (Counterclockwise)

Preceding gene: 21227098

Following gene: 21227096

Centisome position: 28.6

GC content: 48.37

Gene sequence:

>798_bases
ATGGCAGAGTCACAATCGCAATCGCAAGATAAGGCATCCGGAAAACTCTATGTAGTAGGGATAGGACCGGGCTCAGTTGA
ACAGTTAACCCTCAAAGCCCGGGATGTAATCCTCAATGCCGATTATGTTCTTGGAAACAGCACTTACCTGGACCAGATGG
AAAGCCTTCTCGGCTCTCAGGAAGTGATCCGCAGTTATATGGGAAAAGAGGTCGAAAGGGCACGAAAAGCTGTGGAACTT
GCAAAAACCGCAAATGTCGTCATGATCAGTGGTGGGGACACCAATGTCTACGGCATGGCAGGAATAGTGCTTGAGGTAGC
AGAACACGAGAACCTTGATGTGGACATTGAAATCCTTCCAGGGGTCACAGCGGTCCTTGCCGGGGCAAGCCTCCTGGGCG
CACCAGTTGTTACGGATTTTGCGGTGATCAGTTTAAGCGACCTCTTAACTCCATGGGAAGTAATTGAAAAGCGGCTTAAT
ATGGCTGCGGATGCCGACTTTGTGATAGGTCTTTACAACCCGAAGAGCCGCAAGAGAAAATCCAATTTTGCAAGGGCAAT
TGAAATTATTCGCAGGTACAAAGCCGATTCCGTACCTGTAGGGCTTGTAAAGAACGCCATGAGAGGCGAGGAAGAAGACC
AGATCGTGACGACCCTTGGGGAAGTCATGAATTATGAGGACTGGGTGGACATGAGCACTGCTATTCTCATAGGCAACGGA
GAGTCCAGGATCTGGAAGTCTCCGAAAAAGGATATAATAATAACTCCCAGGGGGTATCATAAGAAATATGACTACTGA

Upstream 100 bases:

>100_bases
TCATCCTCCCAGGCTTCGCGCTTCGGATTAAAAGGGGTTGCAGAGCCTGCTGCTCTGGCTCTCTCGGAAAAACACGAATT
GATTTGCAGGAAGAAAGTCT

Downstream 100 bases:

>100_bases
ACAAAAAGCCAATGAAAATGCAGGAAACCTTGAAAACCTCGAAGAATTTACCGAGCTTACAGTGGAAGTAGACCCCGAAC
TTGTAAAGATCTGCAATGAC

Product: precorrin-3B C17-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 4

Alternate protein names: Cobalt-precorrin-3B C(17)-methyltransferase; Cobalt-precorrin-3 methylase; Cobalt-precorrin-3 methyltransferase; Cobalt-precorrin-8X methylmutase; Cobalt-precorrin isomerase [H]

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MAESQSQSQDKASGKLYVVGIGPGSVEQLTLKARDVILNADYVLGNSTYLDQMESLLGSQEVIRSYMGKEVERARKAVEL
AKTANVVMISGGDTNVYGMAGIVLEVAEHENLDVDIEILPGVTAVLAGASLLGAPVVTDFAVISLSDLLTPWEVIEKRLN
MAADADFVIGLYNPKSRKRKSNFARAIEIIRRYKADSVPVGLVKNAMRGEEEDQIVTTLGEVMNYEDWVDMSTAILIGNG
ESRIWKSPKKDIIITPRGYHKKYDY

Sequences:

>Translated_265_residues
MAESQSQSQDKASGKLYVVGIGPGSVEQLTLKARDVILNADYVLGNSTYLDQMESLLGSQEVIRSYMGKEVERARKAVEL
AKTANVVMISGGDTNVYGMAGIVLEVAEHENLDVDIEILPGVTAVLAGASLLGAPVVTDFAVISLSDLLTPWEVIEKRLN
MAADADFVIGLYNPKSRKRKSNFARAIEIIRRYKADSVPVGLVKNAMRGEEEDQIVTTLGEVMNYEDWVDMSTAILIGNG
ESRIWKSPKKDIIITPRGYHKKYDY
>Mature_264_residues
AESQSQSQDKASGKLYVVGIGPGSVEQLTLKARDVILNADYVLGNSTYLDQMESLLGSQEVIRSYMGKEVERARKAVELA
KTANVVMISGGDTNVYGMAGIVLEVAEHENLDVDIEILPGVTAVLAGASLLGAPVVTDFAVISLSDLLTPWEVIEKRLNM
AADADFVIGLYNPKSRKRKSNFARAIEIIRRYKADSVPVGLVKNAMRGEEEDQIVTTLGEVMNYEDWVDMSTAILIGNGE
SRIWKSPKKDIIITPRGYHKKYDY

Specific function: Bifunctional enzyme with a methyltransferase domain that catalyzes the methylation of C-17 in cobalt-precorrin-3B to form cobalt-precorrin-4 and an isomerase domain that catalyzes the conversion of cobalt-precorrin-8X to cobyrinic acid; a methyl migration

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the CobH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014422
- InterPro:   IPR003722
- InterPro:   IPR006363 [H]

Pfam domain/function: PF02570 CbiC; PF00590 TP_methylase [H]

EC number: 2.1.1.131

Molecular weight: Translated: 29088; Mature: 28956

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAESQSQSQDKASGKLYVVGIGPGSVEQLTLKARDVILNADYVLGNSTYLDQMESLLGSQ
CCCCCCCCHHHCCCEEEEEEECCCCCHHHEEEHHHEEEECCEEECCCHHHHHHHHHHCHH
EVIRSYMGKEVERARKAVELAKTANVVMISGGDTNVYGMAGIVLEVAEHENLDVDIEILP
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHEEECCCCCCCEEEEECC
GVTAVLAGASLLGAPVVTDFAVISLSDLLTPWEVIEKRLNMAADADFVIGLYNPKSRKRK
CHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHH
SNFARAIEIIRRYKADSVPVGLVKNAMRGEEEDQIVTTLGEVMNYEDWVDMSTAILIGNG
HHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCHHHCCCEEEEEEECC
ESRIWKSPKKDIIITPRGYHKKYDY
CHHHCCCCCCCEEECCCCCCCCCCC
>Mature Secondary Structure 
AESQSQSQDKASGKLYVVGIGPGSVEQLTLKARDVILNADYVLGNSTYLDQMESLLGSQ
CCCCCCCHHHCCCEEEEEEECCCCCHHHEEEHHHEEEECCEEECCCHHHHHHHHHHCHH
EVIRSYMGKEVERARKAVELAKTANVVMISGGDTNVYGMAGIVLEVAEHENLDVDIEILP
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHEEECCCCCCCEEEEECC
GVTAVLAGASLLGAPVVTDFAVISLSDLLTPWEVIEKRLNMAADADFVIGLYNPKSRKRK
CHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHH
SNFARAIEIIRRYKADSVPVGLVKNAMRGEEEDQIVTTLGEVMNYEDWVDMSTAILIGNG
HHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCHHHCCCEEEEEEECC
ESRIWKSPKKDIIITPRGYHKKYDY
CHHHCCCCCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 3B

Specific reaction: S-adenosyl-L-methionine + precorrin-3B = S-adenosyl-L-homocysteine + precorrin-4

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]