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The map label for this gene is amt [H]

Identifier: 21226835

GI number: 21226835

Start: 881714

End: 882913

Strand: Direct

Name: amt [H]

Synonym: MM_0733

Alternate gene names: 21226835

Gene position: 881714-882913 (Clockwise)

Preceding gene: 21226834

Following gene: 21226836

Centisome position: 21.52

GC content: 50.67

Gene sequence:

>1200_bases
TTGAAAATGGCTATAAACGGAGCAGACACTGTATGGGTATTGCTTTCATCGGCATTGGTATTATTAATGGTTCCTGCGCT
GGCACTGTTCTACGGAGGGCTTGTCCAGCGTAAAAACGTCCTGAGCAGCATGATGCATTCATTCGTTGCAATGGGCGTCC
TGGCTCTTGAATGGATAATAATAGGTTACTCCCTTGCGTTTTCAGAAGGCAACTGGTTTATTGGCGGCCTTGAGAATGTG
TTTCTGAGCGGGATTGATCCTTACTCCGTAACAGGTACGATTCCCACCTATGCATTTGTTGCATTCCAGGGAATGTTCGC
AATAATTACTCCTGCACTTATATCCGGTGCCATAGTAGGGCGTGTCAAGTTCAAGTCTTACATCGCGTTCATCCTGCTCT
GGGGCCTTATTGTCTATGCCCCGGTATGTCACTGGGTTTGGGGCGGCGGATTCCTTACCGGAGAAGCCCTTGACTTTGCA
GGCGGTACGGTTGTCCACATAACTGCAGGTATGTCTTCACTTGCAATACTGGCATACCTCGGCAAGAGAAGGGGATATGG
TGTTGATTCCCTGAAGCCGCACAACGTTACCCTGACCCTCCTCGGAGCCGGACTTCTCTGGTTCGGGTGGTTCGGGTTCA
ACGCAGGCTCGGCTCTTGCTGCAAACGAAATAGCAGCCCTTGCATTCATGACCACGCTCGTTGCTCCCGCTGCAGCAGGT
TTTGTCTGGATGGCGCTTGAATGGGCGCACCTTGGACGCCCCACCGCACTCGGGTTTGCAACCGGCATCCTGGCAGGGCT
TGTAGGGATAACTCCTGCTGCCGGATTTGTCACTCCAGTAGCCGCGATTGCCATAGGTGCGATTGCAAGCTTCATATGTT
ACCAGGCTGTGATGTTCAAAAACAGGCTAGGATACGACGATTCACTTGATGCATTTGGAGTTCACGGAGTCGGCGGAATA
GTCGGTGCGATATTAACAGGCGTATATGCAAGTGTAGGCGGAGAAGGCCTACTTCTCGGGAACTCTGCCCAGTTGATGAT
TCAGCTTCAGGGCGTGGTTATCACAATTGTATACGCAATGGCCTGCACCCTGCTGATAGGATTTGTACTCCATAAAACGA
TAGGGCTCAAAGTCAGTGAAAGCGAAGAAATCATAGGGCTTGACAAAACCCAGCATGGTGAAGCAGCATACAACATTTGA

Upstream 100 bases:

>100_bases
AGTGACGGTTACGTTGGAAGCGGAAAGATCTTCGTGCTGCCACTTGAAAACGTGATAAGGATCCGCACGAACGAAACCGG
ACCTGAGGCAATTTGAGGGG

Downstream 100 bases:

>100_bases
GTTCGGGTGAATCTTCACCCTGGCTCTCAAACAGATTTTTTTATTTCTTAACTGTTTTTTATTTCTTAACTGTTTTTTAT
TTCTTAACTGTTTTATATTT

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MKMAINGADTVWVLLSSALVLLMVPALALFYGGLVQRKNVLSSMMHSFVAMGVLALEWIIIGYSLAFSEGNWFIGGLENV
FLSGIDPYSVTGTIPTYAFVAFQGMFAIITPALISGAIVGRVKFKSYIAFILLWGLIVYAPVCHWVWGGGFLTGEALDFA
GGTVVHITAGMSSLAILAYLGKRRGYGVDSLKPHNVTLTLLGAGLLWFGWFGFNAGSALAANEIAALAFMTTLVAPAAAG
FVWMALEWAHLGRPTALGFATGILAGLVGITPAAGFVTPVAAIAIGAIASFICYQAVMFKNRLGYDDSLDAFGVHGVGGI
VGAILTGVYASVGGEGLLLGNSAQLMIQLQGVVITIVYAMACTLLIGFVLHKTIGLKVSESEEIIGLDKTQHGEAAYNI

Sequences:

>Translated_399_residues
MKMAINGADTVWVLLSSALVLLMVPALALFYGGLVQRKNVLSSMMHSFVAMGVLALEWIIIGYSLAFSEGNWFIGGLENV
FLSGIDPYSVTGTIPTYAFVAFQGMFAIITPALISGAIVGRVKFKSYIAFILLWGLIVYAPVCHWVWGGGFLTGEALDFA
GGTVVHITAGMSSLAILAYLGKRRGYGVDSLKPHNVTLTLLGAGLLWFGWFGFNAGSALAANEIAALAFMTTLVAPAAAG
FVWMALEWAHLGRPTALGFATGILAGLVGITPAAGFVTPVAAIAIGAIASFICYQAVMFKNRLGYDDSLDAFGVHGVGGI
VGAILTGVYASVGGEGLLLGNSAQLMIQLQGVVITIVYAMACTLLIGFVLHKTIGLKVSESEEIIGLDKTQHGEAAYNI
>Mature_399_residues
MKMAINGADTVWVLLSSALVLLMVPALALFYGGLVQRKNVLSSMMHSFVAMGVLALEWIIIGYSLAFSEGNWFIGGLENV
FLSGIDPYSVTGTIPTYAFVAFQGMFAIITPALISGAIVGRVKFKSYIAFILLWGLIVYAPVCHWVWGGGFLTGEALDFA
GGTVVHITAGMSSLAILAYLGKRRGYGVDSLKPHNVTLTLLGAGLLWFGWFGFNAGSALAANEIAALAFMTTLVAPAAAG
FVWMALEWAHLGRPTALGFATGILAGLVGITPAAGFVTPVAAIAIGAIASFICYQAVMFKNRLGYDDSLDAFGVHGVGGI
VGAILTGVYASVGGEGLLLGNSAQLMIQLQGVVITIVYAMACTLLIGFVLHKTIGLKVSESEEIIGLDKTQHGEAAYNI

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=403, Percent_Identity=46.8982630272953, Blast_Score=328, Evalue=5e-91,
Organism=Caenorhabditis elegans, GI32564042, Length=418, Percent_Identity=29.1866028708134, Blast_Score=130, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI133931100, Length=435, Percent_Identity=27.5862068965517, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17550180, Length=426, Percent_Identity=28.169014084507, Blast_Score=121, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17550182, Length=441, Percent_Identity=28.7981859410431, Blast_Score=114, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6324187, Length=417, Percent_Identity=37.8896882494005, Blast_Score=238, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6325396, Length=404, Percent_Identity=34.6534653465347, Blast_Score=221, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6321559, Length=415, Percent_Identity=33.0120481927711, Blast_Score=212, Evalue=8e-56,
Organism=Drosophila melanogaster, GI161078314, Length=410, Percent_Identity=31.9512195121951, Blast_Score=143, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 41772; Mature: 41772

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMAINGADTVWVLLSSALVLLMVPALALFYGGLVQRKNVLSSMMHSFVAMGVLALEWII
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGYSLAFSEGNWFIGGLENVFLSGIDPYSVTGTIPTYAFVAFQGMFAIITPALISGAIVG
HHHHEEECCCCEEECCHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RVKFKSYIAFILLWGLIVYAPVCHWVWGGGFLTGEALDFAGGTVVHITAGMSSLAILAYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHCCCCEEEEEECCHHHHHHHHHH
GKRRGYGVDSLKPHNVTLTLLGAGLLWFGWFGFNAGSALAANEIAALAFMTTLVAPAAAG
HHCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FVWMALEWAHLGRPTALGFATGILAGLVGITPAAGFVTPVAAIAIGAIASFICYQAVMFK
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRLGYDDSLDAFGVHGVGGIVGAILTGVYASVGGEGLLLGNSAQLMIQLQGVVITIVYAM
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEHHHHHHHHHHH
ACTLLIGFVLHKTIGLKVSESEEIIGLDKTQHGEAAYNI
HHHHHHHHHHHHHHCCEECCCCCEEECCCCCCCCCCCCC
>Mature Secondary Structure
MKMAINGADTVWVLLSSALVLLMVPALALFYGGLVQRKNVLSSMMHSFVAMGVLALEWII
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGYSLAFSEGNWFIGGLENVFLSGIDPYSVTGTIPTYAFVAFQGMFAIITPALISGAIVG
HHHHEEECCCCEEECCHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RVKFKSYIAFILLWGLIVYAPVCHWVWGGGFLTGEALDFAGGTVVHITAGMSSLAILAYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHCCCCEEEEEECCHHHHHHHHHH
GKRRGYGVDSLKPHNVTLTLLGAGLLWFGWFGFNAGSALAANEIAALAFMTTLVAPAAAG
HHCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FVWMALEWAHLGRPTALGFATGILAGLVGITPAAGFVTPVAAIAIGAIASFICYQAVMFK
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRLGYDDSLDAFGVHGVGGIVGAILTGVYASVGGEGLLLGNSAQLMIQLQGVVITIVYAM
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEHHHHHHHHHHH
ACTLLIGFVLHKTIGLKVSESEEIIGLDKTQHGEAAYNI
HHHHHHHHHHHHHHCCEECCCCCEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]