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The map label for this gene is nifD2 [H]

Identifier: 21226824

GI number: 21226824

Start: 867704

End: 869302

Strand: Direct

Name: nifD2 [H]

Synonym: MM_0722

Alternate gene names: 21226824

Gene position: 867704-869302 (Clockwise)

Preceding gene: 21226823

Following gene: 21226825

Centisome position: 21.18

GC content: 47.22

Gene sequence:

>1599_bases
ATGGGAGCGGAAATTAACTCTGAGATTGGAGACAAACAGCAGCTTGTTGAAAATATGATGAAAGCGCTCCCGGAAAAGGC
TGCAAGAAACAGGAGAAAACACATAGTTGTCAGGGACTGTTCCACCGAACAGCACATTGAAGCCGACGATAAGGTAATCC
CCGGAATCCTTACAAACCGCGGATGTGCTTTTGCTGGCACCAAAGGTGTGGTTTTCGGGCCGATCAAAGACATGGTGCAT
ATCGTCCACGGGCCCATAGGCTGCGCATTTTTCACCTGGGGGACCAGGCGAAACTTTGCAAAGGCAGAAGAGGGCGAAGA
CAACTACATGAATTACTGCGTATGCACGGATATGAAGGAGACCGATATTGTCTTTGGAGGAGAAAAAAAGCTAAAGAAAG
CCATTGACGAAGTTGTAAAAATCTTCAATCCCGAAGCAGTTACCATCTGCGCAACCTGTCCTGTCGGGCTTATAGGAGAC
GACATTGAAGCAGTTTCAAGGGAAGCTGAAAAAGAACACGGTATAAAAGTAATTCCTGCCCGATGTGAAGGGTATAGGGG
AGTCAGCCAGTCAGCCGGACACCATATTGCGAGCAACGCCCTGATGGAGAACCTTATCGGGACCGAGGATATCAAAAACC
CCACCCCGTTTGACATCAACATATTCGGAGAATACAATATCGGAGGAGACCTCTGGGAAATTAAGCCGATCCTTGAAAAA
ATAGGGTACAGGATTGTCTCAACCTTTACCGGAGACGGGTCATTCCATAAACTTGCACAGGCCCATAGAGCAAAACTCAG
CATCCTGCTCTGCCACCGCTCCATCAACTACACAAACCGCATGATGGAAGAGAAATACGGGATTCCCTGGCTGAAGGTCA
ATTACATAGGTACAAAAGCTACTGAAAAGTCACTCAAGAAAATGGCAGAGTTCTTTGACGACCCTGAAATCACCAGAAAA
ACTGAAGAAATCATTGCCGAAGAAACTGCAAAGTACAGAGACGAGATAGAGAAATACAGAAAGAAGCTCCAGGGAAAAAC
CGCCTTTATCTATGCAGGAGGTTCAAGAAGCCATCACTACACGAACCTCTTTGAAGAGCTCGGCATGAAAGTGGTTGTTG
CAGGCTACCAGTTCGCTCACAGGGACGACTATGAGGGCAGACAGATCATTCCCCACATGCAGGAAAAAGCCCTTGGTTCG
ATCCTTGAAGATATACACTACGAAAAGGATGAAAATGTCAAACCGGCAGTCAGTCCTGAAAGGATTGAGGAGCTGAAAAA
GAAAATCGGTCTCATGGACTATGAAGGGCTCTTCCCGGAAATGAAAGATGGGACCATTGTTATAGATGACCTCAACCACC
ACGAAACCGAAGCCCTCCTGAAAACCCTTAAACCTGACATTTTCTGCTCAGGGATCAAGGATAAGTACTGGGCTCAAAAG
CACGGGATCCCTTCAAGGCAGATCCATTCCTATGACTACAGCGGACGCTATACCGGTTTTTCCGGAGTTGTAAATTTCGC
AAGGGACATAGACATGGCTATGCACAGCCCCACCTGGAGGTTCATACGCCCGCCCTGGAAAGGAGAAGGAGAAGAATAA

Upstream 100 bases:

>100_bases
CCGGGAGACGGAAAAATATTCGTATCAAGTATCAGCGAAGCATACCGCATAAGGACCGGAGAAAGCGGAGAAGCAACCGT
GAATAAGGAGATGGTGTGAT

Downstream 100 bases:

>100_bases
GGAGGGACGAAAAATGTTAGACTATACCCCATGTGAAGAGATAACCAGAGAAGCAGTAACCATCAATCCTGCAAAGATCT
GCCAGCCAATAGGTGCGGTA

Product: nitrogenase molybdenum-iron protein subunit alpha

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MGAEINSEIGDKQQLVENMMKALPEKAARNRRKHIVVRDCSTEQHIEADDKVIPGILTNRGCAFAGTKGVVFGPIKDMVH
IVHGPIGCAFFTWGTRRNFAKAEEGEDNYMNYCVCTDMKETDIVFGGEKKLKKAIDEVVKIFNPEAVTICATCPVGLIGD
DIEAVSREAEKEHGIKVIPARCEGYRGVSQSAGHHIASNALMENLIGTEDIKNPTPFDINIFGEYNIGGDLWEIKPILEK
IGYRIVSTFTGDGSFHKLAQAHRAKLSILLCHRSINYTNRMMEEKYGIPWLKVNYIGTKATEKSLKKMAEFFDDPEITRK
TEEIIAEETAKYRDEIEKYRKKLQGKTAFIYAGGSRSHHYTNLFEELGMKVVVAGYQFAHRDDYEGRQIIPHMQEKALGS
ILEDIHYEKDENVKPAVSPERIEELKKKIGLMDYEGLFPEMKDGTIVIDDLNHHETEALLKTLKPDIFCSGIKDKYWAQK
HGIPSRQIHSYDYSGRYTGFSGVVNFARDIDMAMHSPTWRFIRPPWKGEGEE

Sequences:

>Translated_532_residues
MGAEINSEIGDKQQLVENMMKALPEKAARNRRKHIVVRDCSTEQHIEADDKVIPGILTNRGCAFAGTKGVVFGPIKDMVH
IVHGPIGCAFFTWGTRRNFAKAEEGEDNYMNYCVCTDMKETDIVFGGEKKLKKAIDEVVKIFNPEAVTICATCPVGLIGD
DIEAVSREAEKEHGIKVIPARCEGYRGVSQSAGHHIASNALMENLIGTEDIKNPTPFDINIFGEYNIGGDLWEIKPILEK
IGYRIVSTFTGDGSFHKLAQAHRAKLSILLCHRSINYTNRMMEEKYGIPWLKVNYIGTKATEKSLKKMAEFFDDPEITRK
TEEIIAEETAKYRDEIEKYRKKLQGKTAFIYAGGSRSHHYTNLFEELGMKVVVAGYQFAHRDDYEGRQIIPHMQEKALGS
ILEDIHYEKDENVKPAVSPERIEELKKKIGLMDYEGLFPEMKDGTIVIDDLNHHETEALLKTLKPDIFCSGIKDKYWAQK
HGIPSRQIHSYDYSGRYTGFSGVVNFARDIDMAMHSPTWRFIRPPWKGEGEE
>Mature_531_residues
GAEINSEIGDKQQLVENMMKALPEKAARNRRKHIVVRDCSTEQHIEADDKVIPGILTNRGCAFAGTKGVVFGPIKDMVHI
VHGPIGCAFFTWGTRRNFAKAEEGEDNYMNYCVCTDMKETDIVFGGEKKLKKAIDEVVKIFNPEAVTICATCPVGLIGDD
IEAVSREAEKEHGIKVIPARCEGYRGVSQSAGHHIASNALMENLIGTEDIKNPTPFDINIFGEYNIGGDLWEIKPILEKI
GYRIVSTFTGDGSFHKLAQAHRAKLSILLCHRSINYTNRMMEEKYGIPWLKVNYIGTKATEKSLKKMAEFFDDPEITRKT
EEIIAEETAKYRDEIEKYRKKLQGKTAFIYAGGSRSHHYTNLFEELGMKVVVAGYQFAHRDDYEGRQIIPHMQEKALGSI
LEDIHYEKDENVKPAVSPERIEELKKKIGLMDYEGLFPEMKDGTIVIDDLNHHETEALLKTLKPDIFCSGIKDKYWAQKH
GIPSRQIHSYDYSGRYTGFSGVVNFARDIDMAMHSPTWRFIRPPWKGEGEE

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR010143
- InterPro:   IPR000318
- InterPro:   IPR005972 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 60306; Mature: 60174

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAEINSEIGDKQQLVENMMKALPEKAARNRRKHIVVRDCSTEQHIEADDKVIPGILTNR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHHCCC
GCAFAGTKGVVFGPIKDMVHIVHGPIGCAFFTWGTRRNFAKAEEGEDNYMNYCVCTDMKE
CCEEECCCCEEECCHHHHHHHHHCCHHHEEEECCCCCCCCCCCCCCCCCEEEEEECCCCC
TDIVFGGEKKLKKAIDEVVKIFNPEAVTICATCPVGLIGDDIEAVSREAEKEHGIKVIPA
CCEEECCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEC
RCEGYRGVSQSAGHHIASNALMENLIGTEDIKNPTPFDINIFGEYNIGGDLWEIKPILEK
CCCCCCCCCHHCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHH
IGYRIVSTFTGDGSFHKLAQAHRAKLSILLCHRSINYTNRMMEEKYGIPWLKVNYIGTKA
CCCEEEEEECCCCHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCHH
TEKSLKKMAEFFDDPEITRKTEEIIAEETAKYRDEIEKYRKKLQGKTAFIYAGGSRSHHY
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHH
TNLFEELGMKVVVAGYQFAHRDDYEGRQIIPHMQEKALGSILEDIHYEKDENVKPAVSPE
HHHHHHCCCEEEEECCHHHCCCCCCCCHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
RIEELKKKIGLMDYEGLFPEMKDGTIVIDDLNHHETEALLKTLKPDIFCSGIKDKYWAQK
HHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEECCCCHHHHHHH
HGIPSRQIHSYDYSGRYTGFSGVVNFARDIDMAMHSPTWRFIRPPWKGEGEE
CCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCC
>Mature Secondary Structure 
GAEINSEIGDKQQLVENMMKALPEKAARNRRKHIVVRDCSTEQHIEADDKVIPGILTNR
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHHCCC
GCAFAGTKGVVFGPIKDMVHIVHGPIGCAFFTWGTRRNFAKAEEGEDNYMNYCVCTDMKE
CCEEECCCCEEECCHHHHHHHHHCCHHHEEEECCCCCCCCCCCCCCCCCEEEEEECCCCC
TDIVFGGEKKLKKAIDEVVKIFNPEAVTICATCPVGLIGDDIEAVSREAEKEHGIKVIPA
CCEEECCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEC
RCEGYRGVSQSAGHHIASNALMENLIGTEDIKNPTPFDINIFGEYNIGGDLWEIKPILEK
CCCCCCCCCHHCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHH
IGYRIVSTFTGDGSFHKLAQAHRAKLSILLCHRSINYTNRMMEEKYGIPWLKVNYIGTKA
CCCEEEEEECCCCHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCHH
TEKSLKKMAEFFDDPEITRKTEEIIAEETAKYRDEIEKYRKKLQGKTAFIYAGGSRSHHY
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHH
TNLFEELGMKVVVAGYQFAHRDDYEGRQIIPHMQEKALGSILEDIHYEKDENVKPAVSPE
HHHHHHCCCEEEEECCHHHCCCCCCCCHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
RIEELKKKIGLMDYEGLFPEMKDGTIVIDDLNHHETEALLKTLKPDIFCSGIKDKYWAQK
HHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEECCCCHHHHHHH
HGIPSRQIHSYDYSGRYTGFSGVVNFARDIDMAMHSPTWRFIRPPWKGEGEE
CCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961410 [H]