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The map label for this gene is purF [H]

Identifier: 21226443

GI number: 21226443

Start: 448215

End: 449672

Strand: Direct

Name: purF [H]

Synonym: MM_0341

Alternate gene names: 21226443

Gene position: 448215-449672 (Clockwise)

Preceding gene: 21226442

Following gene: 21226451

Centisome position: 10.94

GC content: 48.83

Gene sequence:

>1458_bases
TTGAAAGAAGAGTGCGGGGTTGCAGGCATAATTTTGCCTGATGACAGACCCGCGTCAAATGCTGTCGCATTCAAGCTTTA
TTATGCCCTTTATGCCCTTCAGCACAGAGGACAGGAATCGACAGGCATAATGGTGCACAACGGCTCATGTCCGCTTTCCA
TCAAAGGAATGGGGCTTGTACCTGATGTATATAACAAAGATTCCCTCGGGCGTCTGGTCGGTAATGCAGGAGTCGGGCAT
GTCCGTTACTCCACAACTGGCGGGTCAAAGATTGAAAACTGTCAGCCTTTTATCCTGAACTTCAAGGGCGGAACAGTTGC
CATTGCTCATAATGGGAATCTGGTAAATGCAAGGGAACTGAAGGATGAGCTGGAAAATGAAGGCAGAATTTTTATCAGTG
ATTCAGATACTGAAGTTATTGGCCATCTGCTTGTAAAAGAACTGATAAAACACGAACCTATCGAGTCTATCAGGACTGTA
ATGCGCAAGCTTGTTGGCTCTTACTCTCTTGTCATTTTCATTAACGGTGTTATCTACGCCGTAAGGGATCCTTTCGGTTT
TAAGCCACTCTGTTTTGGAGAAGTTGACGGCGGATACGGAGTATTTTCCGAGAGCGTAGCCATTGACACCCTGAACGGCA
CCCTTATAAGGGACGTCAAACCAGGCGAGGTCATGGTCTTTACAGGCTCAGGTTTTGAAAGCCACCAGCTCGCAAATGAA
CCGCATCCGGCACATTGCGTTTTTGAGTTCATTTACTTTGCAAGGCCAGACTCCGTTATAGACGGCAAGCTTGTCTATAA
AGTAAGGGAAAGAATCGGAAGGGAGCTTGCCCGCGAACATCATGTAGACGCAGACATCGTGTCTCCTGTCCCTGACTCCG
GAATCACTTCCGCAATCGGCTATGCAAGGGAATCAGGTATAATGTACCTGGAAGGGCTCATGAAGAACCGTTATATCGGG
CGCACCTTCATTCTTCCGGGACAGGACCTGCGTGAAACTGCAGTTCGGCTTAAGATGAACGCTATTCAGGACAATATCAA
AGGCAAACGTGTTGTCCTGGTAGATGACAGCATTGTCCGCGGGACTACCTCGAGGCGTATCATAGATATGGTCAGGAAGG
CAGGCGCGTCAGAAGTCCACGCAAGGGTCGGAAGCCCGGCAATTATTGCCCCCTGCTACCTGGGAATCGATATGGCAACC
CGGCAGGAACTTATCGCTTCATACAAGACCATAAAAGAGGTCGAAGGGCTGATAAGTGCTGATTCTCTCGGCTACCTCAG
CATCGACGGGCTTATGAGAGCACTTGAGTGCGACAAGAACGACATGTGCATTGGCTGCCTTACAGGAGAATATCCTGTAG
AGATCCCGGGCGAGAAATGCAGAAAGAAACAGACCCGTCTGGACGATTTCAGTAAGGCTGAAAGATCCTCTCAGGCAGTA
TCTCCGGACAGCCTTTAA

Upstream 100 bases:

>100_bases
AAGACATCCCGTATGAGAACTTACAAGTGGCAGGCAAAGTGCAAATACTAAACCTGCCTGCTCAATTTTCTCTATTTTTT
ACCTGCACCGGAGGTCAAAA

Downstream 100 bases:

>100_bases
GGATTTTTTCACATTTCTTAATTTCTTCTTTCTTAATTTTTTATATTTTTATTTTTTATTCCTTAATTTTTTTACTCAAT
TTTTTCTGCCTTTATTCAGT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MKEECGVAGIILPDDRPASNAVAFKLYYALYALQHRGQESTGIMVHNGSCPLSIKGMGLVPDVYNKDSLGRLVGNAGVGH
VRYSTTGGSKIENCQPFILNFKGGTVAIAHNGNLVNARELKDELENEGRIFISDSDTEVIGHLLVKELIKHEPIESIRTV
MRKLVGSYSLVIFINGVIYAVRDPFGFKPLCFGEVDGGYGVFSESVAIDTLNGTLIRDVKPGEVMVFTGSGFESHQLANE
PHPAHCVFEFIYFARPDSVIDGKLVYKVRERIGRELAREHHVDADIVSPVPDSGITSAIGYARESGIMYLEGLMKNRYIG
RTFILPGQDLRETAVRLKMNAIQDNIKGKRVVLVDDSIVRGTTSRRIIDMVRKAGASEVHARVGSPAIIAPCYLGIDMAT
RQELIASYKTIKEVEGLISADSLGYLSIDGLMRALECDKNDMCIGCLTGEYPVEIPGEKCRKKQTRLDDFSKAERSSQAV
SPDSL

Sequences:

>Translated_485_residues
MKEECGVAGIILPDDRPASNAVAFKLYYALYALQHRGQESTGIMVHNGSCPLSIKGMGLVPDVYNKDSLGRLVGNAGVGH
VRYSTTGGSKIENCQPFILNFKGGTVAIAHNGNLVNARELKDELENEGRIFISDSDTEVIGHLLVKELIKHEPIESIRTV
MRKLVGSYSLVIFINGVIYAVRDPFGFKPLCFGEVDGGYGVFSESVAIDTLNGTLIRDVKPGEVMVFTGSGFESHQLANE
PHPAHCVFEFIYFARPDSVIDGKLVYKVRERIGRELAREHHVDADIVSPVPDSGITSAIGYARESGIMYLEGLMKNRYIG
RTFILPGQDLRETAVRLKMNAIQDNIKGKRVVLVDDSIVRGTTSRRIIDMVRKAGASEVHARVGSPAIIAPCYLGIDMAT
RQELIASYKTIKEVEGLISADSLGYLSIDGLMRALECDKNDMCIGCLTGEYPVEIPGEKCRKKQTRLDDFSKAERSSQAV
SPDSL
>Mature_485_residues
MKEECGVAGIILPDDRPASNAVAFKLYYALYALQHRGQESTGIMVHNGSCPLSIKGMGLVPDVYNKDSLGRLVGNAGVGH
VRYSTTGGSKIENCQPFILNFKGGTVAIAHNGNLVNARELKDELENEGRIFISDSDTEVIGHLLVKELIKHEPIESIRTV
MRKLVGSYSLVIFINGVIYAVRDPFGFKPLCFGEVDGGYGVFSESVAIDTLNGTLIRDVKPGEVMVFTGSGFESHQLANE
PHPAHCVFEFIYFARPDSVIDGKLVYKVRERIGRELAREHHVDADIVSPVPDSGITSAIGYARESGIMYLEGLMKNRYIG
RTFILPGQDLRETAVRLKMNAIQDNIKGKRVVLVDDSIVRGTTSRRIIDMVRKAGASEVHARVGSPAIIAPCYLGIDMAT
RQELIASYKTIKEVEGLISADSLGYLSIDGLMRALECDKNDMCIGCLTGEYPVEIPGEKCRKKQTRLDDFSKAERSSQAV
SPDSL

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=508, Percent_Identity=40.9448818897638, Blast_Score=365, Evalue=1e-101,
Organism=Escherichia coli, GI1788651, Length=462, Percent_Identity=41.3419913419913, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI1790167, Length=171, Percent_Identity=29.8245614035088, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17554892, Length=481, Percent_Identity=41.5800415800416, Blast_Score=340, Evalue=7e-94,
Organism=Saccharomyces cerevisiae, GI6323958, Length=473, Percent_Identity=36.7864693446089, Blast_Score=270, Evalue=4e-73,
Organism=Drosophila melanogaster, GI28573187, Length=494, Percent_Identity=43.5222672064777, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24659598, Length=491, Percent_Identity=42.3625254582485, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24659604, Length=462, Percent_Identity=43.0735930735931, Blast_Score=368, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 53118; Mature: 53118

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEECGVAGIILPDDRPASNAVAFKLYYALYALQHRGQESTGIMVHNGSCPLSIKGMGLV
CCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEECCCCCC
PDVYNKDSLGRLVGNAGVGHVRYSTTGGSKIENCQPFILNFKGGTVAIAHNGNLVNAREL
CCCCCCCHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEEECCCEEEEEECCCCCCHHHH
KDELENEGRIFISDSDTEVIGHLLVKELIKHEPIESIRTVMRKLVGSYSLVIFINGVIYA
HHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEEEEEEEEE
VRDPFGFKPLCFGEVDGGYGVFSESVAIDTLNGTLIRDVKPGEVMVFTGSGFESHQLANE
EECCCCCCCEEEEECCCCCCEEECCEEEEECCCEEEEECCCCEEEEEECCCCCHHHCCCC
PHPAHCVFEFIYFARPDSVIDGKLVYKVRERIGRELAREHHVDADIVSPVPDSGITSAIG
CCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCHHHHHH
YARESGIMYLEGLMKNRYIGRTFILPGQDLRETAVRLKMNAIQDNIKGKRVVLVDDSIVR
HHHHCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHC
GTTSRRIIDMVRKAGASEVHARVGSPAIIAPCYLGIDMATRQELIASYKTIKEVEGLISA
CCCHHHHHHHHHHCCCHHHHHHCCCCCEEEHHEECCCHHHHHHHHHHHHHHHHHHHHHCC
DSLGYLSIDGLMRALECDKNDMCIGCLTGEYPVEIPGEKCRKKQTRLDDFSKAERSSQAV
CCCCEEEHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCHHHHHHHHHCCCC
SPDSL
CCCCC
>Mature Secondary Structure
MKEECGVAGIILPDDRPASNAVAFKLYYALYALQHRGQESTGIMVHNGSCPLSIKGMGLV
CCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEECCCCCC
PDVYNKDSLGRLVGNAGVGHVRYSTTGGSKIENCQPFILNFKGGTVAIAHNGNLVNAREL
CCCCCCCHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEEECCCEEEEEECCCCCCHHHH
KDELENEGRIFISDSDTEVIGHLLVKELIKHEPIESIRTVMRKLVGSYSLVIFINGVIYA
HHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEEEEEEEEE
VRDPFGFKPLCFGEVDGGYGVFSESVAIDTLNGTLIRDVKPGEVMVFTGSGFESHQLANE
EECCCCCCCEEEEECCCCCCEEECCEEEEECCCEEEEECCCCEEEEEECCCCCHHHCCCC
PHPAHCVFEFIYFARPDSVIDGKLVYKVRERIGRELAREHHVDADIVSPVPDSGITSAIG
CCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCHHHHHH
YARESGIMYLEGLMKNRYIGRTFILPGQDLRETAVRLKMNAIQDNIKGKRVVLVDDSIVR
HHHHCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHC
GTTSRRIIDMVRKAGASEVHARVGSPAIIAPCYLGIDMATRQELIASYKTIKEVEGLISA
CCCHHHHHHHHHHCCCHHHHHHCCCCCEEEHHEECCCHHHHHHHHHHHHHHHHHHHHHCC
DSLGYLSIDGLMRALECDKNDMCIGCLTGEYPVEIPGEKCRKKQTRLDDFSKAERSSQAV
CCCCEEEHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCHHHHHHHHHCCCC
SPDSL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]