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The map label for this gene is aspC [H]

Identifier: 21226176

GI number: 21226176

Start: 99386

End: 100720

Strand: Reverse

Name: aspC [H]

Synonym: MM_0074

Alternate gene names: 21226176

Gene position: 100720-99386 (Counterclockwise)

Preceding gene: 21226180

Following gene: 21226175

Centisome position: 2.46

GC content: 48.84

Gene sequence:

>1335_bases
ATGTCTTTAGCAAATCTCAGAACGCATTATACAGCCGATGTCAGACCGGATAAGGTTGAGAACGGTCAGAAAGTAACCCT
TGCAGGCTGGGTCCATGAGGTCAGAGACCTCGGAGGGATCTGTTTTGTCGTGCTCCGGGACAGGGAAGGAAAGGCTCAGG
TCACACTTGTAAAGAAAAAGATCGATAAAGATCTCTTTGATGCAGCCAGAAGGCTCGTTCGCGAGTCCGTAATTTCCGTG
ACCGGCTCTGTCAAGTTTGAAGAAAAAGCACCGAACGGCTATGAACTTCTCCCTGACGAGATCAATGTCCTGAACGTCTC
AGGTTCTCCTCTTCCAATGGACACCACCGGAAAGGTTGAAGCAGAACTCGACACAAGGCTTGACTCCCGCTTCATCGATC
TGAGAAGAGCAGAGACAACTGCGGTCTTCAAGATAAGACACGAATCCCTCCGGGCTATAAGGGAATACTTTGTAAAATAC
AACTTTATAGAAACCGCAACTCCAAAGGTCGTTGCTACAGCAACAGAAGGCGGAACTGCCCTTTTCCCGATAACCTATTT
TGACAGGGAAGCTTTTCTCAACCAGAGCCCACAGCTTTTCAAGCAGATCCTTATGAGCGGCGGGTTTGACAGGGTCTTTG
AAATAGGGCCGATTTTCAGGGCAGAAGAACATGATACACGCAGGCACTTAAACGAAGCCACTTCTATTGACGTGGAGGTC
AGTTTTGCAGACCATTTTGATGTAATGGAGCTCCTGGAAAACCTTGTGGCTTATGTTTACACGCGAGTGATCGAGAACTG
CAAATCCTCCCTTGAAGTTCTCGGGGTTGACCTGAAGGTTCCTAAGACTCCTTTCCTCAAGCTCACCTATGATGAAGTGA
TAGAGATAATAAACAGCCGCTGTGAAGAGAAAATGCACTGGGGAGATGACCTGGGCACACTTGGAGAGCACACCGTCGGA
GACCACGTTTACGAGACCACAGGTGAGTCCCATTACTTCATTATCGACTGGCCCACGGAAATCAAACCTTTCTATGCCAT
GCCTTACGAAGACAGGCCTGAATTCAGCAAGTCCTTTGATATGATGCACCGCACAATGGAGCTCTCTTCAGGAGCCCAGC
GTATTCACATCCCTTCCCTGCTTAAGAACAGGATTGAGTCCCAGGGTTTAAACCCTGATGGTTTTGAGTTCTACCTCAAA
GCTTTCGAATTCGGGATGCCTCCGCATGCAGGATGGGGAATGGGCTGTGAACGTTTTGTCATGACCATGCTGGGGACCGA
GAATATCCGGGATACCGTACTCTTCCCGAGGGACAGGAGAAGACTTTCTCCCTGA

Upstream 100 bases:

>100_bases
GGTGCAACAAATACATTAATAATGAGTTATGACTATTCACCTACGTTTCAGCTTCATTCTTATATTTACGTTTAATTTAT
CCCCTAATTAAGGTGAATTT

Downstream 100 bases:

>100_bases
TCCCCTCCAATTTTTCATTTAAACTTGGAAGGACTTACTGTTTCTGAAAACATCTGTTGAATATGTTAACTTTTTCAGTT
AACTTTTTTAGAGCTGAAAG

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MSLANLRTHYTADVRPDKVENGQKVTLAGWVHEVRDLGGICFVVLRDREGKAQVTLVKKKIDKDLFDAARRLVRESVISV
TGSVKFEEKAPNGYELLPDEINVLNVSGSPLPMDTTGKVEAELDTRLDSRFIDLRRAETTAVFKIRHESLRAIREYFVKY
NFIETATPKVVATATEGGTALFPITYFDREAFLNQSPQLFKQILMSGGFDRVFEIGPIFRAEEHDTRRHLNEATSIDVEV
SFADHFDVMELLENLVAYVYTRVIENCKSSLEVLGVDLKVPKTPFLKLTYDEVIEIINSRCEEKMHWGDDLGTLGEHTVG
DHVYETTGESHYFIIDWPTEIKPFYAMPYEDRPEFSKSFDMMHRTMELSSGAQRIHIPSLLKNRIESQGLNPDGFEFYLK
AFEFGMPPHAGWGMGCERFVMTMLGTENIRDTVLFPRDRRRLSP

Sequences:

>Translated_444_residues
MSLANLRTHYTADVRPDKVENGQKVTLAGWVHEVRDLGGICFVVLRDREGKAQVTLVKKKIDKDLFDAARRLVRESVISV
TGSVKFEEKAPNGYELLPDEINVLNVSGSPLPMDTTGKVEAELDTRLDSRFIDLRRAETTAVFKIRHESLRAIREYFVKY
NFIETATPKVVATATEGGTALFPITYFDREAFLNQSPQLFKQILMSGGFDRVFEIGPIFRAEEHDTRRHLNEATSIDVEV
SFADHFDVMELLENLVAYVYTRVIENCKSSLEVLGVDLKVPKTPFLKLTYDEVIEIINSRCEEKMHWGDDLGTLGEHTVG
DHVYETTGESHYFIIDWPTEIKPFYAMPYEDRPEFSKSFDMMHRTMELSSGAQRIHIPSLLKNRIESQGLNPDGFEFYLK
AFEFGMPPHAGWGMGCERFVMTMLGTENIRDTVLFPRDRRRLSP
>Mature_443_residues
SLANLRTHYTADVRPDKVENGQKVTLAGWVHEVRDLGGICFVVLRDREGKAQVTLVKKKIDKDLFDAARRLVRESVISVT
GSVKFEEKAPNGYELLPDEINVLNVSGSPLPMDTTGKVEAELDTRLDSRFIDLRRAETTAVFKIRHESLRAIREYFVKYN
FIETATPKVVATATEGGTALFPITYFDREAFLNQSPQLFKQILMSGGFDRVFEIGPIFRAEEHDTRRHLNEATSIDVEVS
FADHFDVMELLENLVAYVYTRVIENCKSSLEVLGVDLKVPKTPFLKLTYDEVIEIINSRCEEKMHWGDDLGTLGEHTVGD
HVYETTGESHYFIIDWPTEIKPFYAMPYEDRPEFSKSFDMMHRTMELSSGAQRIHIPSLLKNRIESQGLNPDGFEFYLKA
FEFGMPPHAGWGMGCERFVMTMLGTENIRDTVLFPRDRRRLSP

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI45439306, Length=451, Percent_Identity=35.2549889135255, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI4758762, Length=434, Percent_Identity=29.9539170506912, Blast_Score=190, Evalue=3e-48,
Organism=Homo sapiens, GI226491502, Length=444, Percent_Identity=28.6036036036036, Blast_Score=165, Evalue=7e-41,
Organism=Homo sapiens, GI194272210, Length=457, Percent_Identity=25.8205689277899, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI5031815, Length=457, Percent_Identity=25.8205689277899, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI40789249, Length=265, Percent_Identity=30.9433962264151, Blast_Score=106, Evalue=4e-23,
Organism=Escherichia coli, GI1787161, Length=466, Percent_Identity=24.8927038626609, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1788173, Length=281, Percent_Identity=35.9430604982206, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1789256, Length=451, Percent_Identity=27.7161862527716, Blast_Score=131, Evalue=9e-32,
Organism=Escherichia coli, GI1790571, Length=450, Percent_Identity=27.3333333333333, Blast_Score=128, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17551876, Length=465, Percent_Identity=33.5483870967742, Blast_Score=255, Evalue=4e-68,
Organism=Caenorhabditis elegans, GI71984122, Length=434, Percent_Identity=31.1059907834101, Blast_Score=192, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI71994340, Length=486, Percent_Identity=26.7489711934156, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17535925, Length=486, Percent_Identity=26.7489711934156, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17535927, Length=486, Percent_Identity=26.7489711934156, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32566633, Length=303, Percent_Identity=29.3729372937294, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17556372, Length=422, Percent_Identity=24.4075829383886, Blast_Score=101, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323011, Length=455, Percent_Identity=34.9450549450549, Blast_Score=262, Evalue=8e-71,
Organism=Saccharomyces cerevisiae, GI6321807, Length=437, Percent_Identity=31.350114416476, Blast_Score=199, Evalue=6e-52,
Organism=Saccharomyces cerevisiae, GI6319872, Length=465, Percent_Identity=24.7311827956989, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6320242, Length=464, Percent_Identity=25.4310344827586, Blast_Score=106, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6325153, Length=256, Percent_Identity=28.125, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17136276, Length=446, Percent_Identity=34.0807174887892, Blast_Score=261, Evalue=5e-70,
Organism=Drosophila melanogaster, GI19921528, Length=432, Percent_Identity=30.787037037037, Blast_Score=179, Evalue=4e-45,
Organism=Drosophila melanogaster, GI19922416, Length=477, Percent_Identity=24.9475890985325, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24640849, Length=482, Percent_Identity=24.896265560166, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24640851, Length=482, Percent_Identity=24.4813278008299, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24584738, Length=299, Percent_Identity=30.4347826086957, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020780
- InterPro:   IPR004523
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 50809; Mature: 50677

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLANLRTHYTADVRPDKVENGQKVTLAGWVHEVRDLGGICFVVLRDREGKAQVTLVKKK
CCCCCCCCEEECCCCCCCCCCCCEEEEHHHHHHHHHCCCEEEEEEECCCCCEEEEHHHHH
IDKDLFDAARRLVRESVISVTGSVKFEEKAPNGYELLPDEINVLNVSGSPLPMDTTGKVE
HCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEECCCCCEEEEECCCCCCCCCCCCEE
AELDTRLDSRFIDLRRAETTAVFKIRHESLRAIREYFVKYNFIETATPKVVATATEGGTA
HHHHHHHHHHHHHHHHCCHHEEEEEHHHHHHHHHHHHHHHCEEECCCCCEEEEECCCCCE
LFPITYFDREAFLNQSPQLFKQILMSGGFDRVFEIGPIFRAEEHDTRRHLNEATSIDVEV
EEEEEEECHHHHHCCCHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHHCEEEEEE
SFADHFDVMELLENLVAYVYTRVIENCKSSLEVLGVDLKVPKTPFLKLTYDEVIEIINSR
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCEEEECHHHHHHHHHHH
CEEKMHWGDDLGTLGEHTVGDHVYETTGESHYFIIDWPTEIKPFYAMPYEDRPEFSKSFD
HHHHHHCCCCCCCCCCCCCCCCHHHCCCCCEEEEEECCCCCCCEEECCCCCCCCHHHHHH
MMHRTMELSSGAQRIHIPSLLKNRIESQGLNPDGFEFYLKAFEFGMPPHAGWGMGCERFV
HHHHHHHHCCCCCEEECHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHH
MTMLGTENIRDTVLFPRDRRRLSP
HHHHCCCCCCCCEECCCHHCCCCC
>Mature Secondary Structure 
SLANLRTHYTADVRPDKVENGQKVTLAGWVHEVRDLGGICFVVLRDREGKAQVTLVKKK
CCCCCCCEEECCCCCCCCCCCCEEEEHHHHHHHHHCCCEEEEEEECCCCCEEEEHHHHH
IDKDLFDAARRLVRESVISVTGSVKFEEKAPNGYELLPDEINVLNVSGSPLPMDTTGKVE
HCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEECCCCCEEEEECCCCCCCCCCCCEE
AELDTRLDSRFIDLRRAETTAVFKIRHESLRAIREYFVKYNFIETATPKVVATATEGGTA
HHHHHHHHHHHHHHHHCCHHEEEEEHHHHHHHHHHHHHHHCEEECCCCCEEEEECCCCCE
LFPITYFDREAFLNQSPQLFKQILMSGGFDRVFEIGPIFRAEEHDTRRHLNEATSIDVEV
EEEEEEECHHHHHCCCHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHHCEEEEEE
SFADHFDVMELLENLVAYVYTRVIENCKSSLEVLGVDLKVPKTPFLKLTYDEVIEIINSR
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCEEEECHHHHHHHHHHH
CEEKMHWGDDLGTLGEHTVGDHVYETTGESHYFIIDWPTEIKPFYAMPYEDRPEFSKSFD
HHHHHHCCCCCCCCCCCCCCCCHHHCCCCCEEEEEECCCCCCCEEECCCCCCCCHHHHHH
MMHRTMELSSGAQRIHIPSLLKNRIESQGLNPDGFEFYLKAFEFGMPPHAGWGMGCERFV
HHHHHHHHCCCCCEEECHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHH
MTMLGTENIRDTVLFPRDRRRLSP
HHHHCCCCCCCCEECCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]