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The map label for this gene is gatE [H]

Identifier: 21226152

GI number: 21226152

Start: 66148

End: 68049

Strand: Reverse

Name: gatE [H]

Synonym: MM_0050

Alternate gene names: 21226152

Gene position: 68049-66148 (Counterclockwise)

Preceding gene: 21226154

Following gene: 21226151

Centisome position: 1.66

GC content: 47.11

Gene sequence:

>1902_bases
ATGGAAAAATACGATTACAGTGAACTTGGGCTGAAAGCCGGGCTTGAAATTCACCAGCAACTGGATTCTAAAGAGAAACT
TTTTTGCAGGTGTCCGACCCTTATAAGGGACATTGAGGATTCGGACTTTGAATTTTTCAGGTACCTCAGGGCTACGGAAA
GTGAGATGGGAGAGAAGGACAGGGCTGCGGTGGAGCAGACCAAAATCAGAAGGAAGTATATTTACAAAGCTTATGACACT
ACCTGCCTTGTCGAAAACGATGAGGAACCGCCAGGGGAACTCAACAGGGAAGCTCTGGACATCTCCCTCGGGGTTGCAAA
GCTTTTCAATATGAAACCCGTAGACCAGATGCATGTGATGAGAAAGATCGTGGTGGACGGGTCAAACACCAGCGGCTTTC
AGAGGACTGCGTTTCTTGCGAGCGACGGAAATATTGAGACTTCGGAAGGGCGGTGCAGAATTGACAGTCTCTGTGTGGAA
GAGGAAGCTGCCCAGAAAATAGAGGAAATCGGAGACTCAATCATTTATTCTCTGGACAGGCTTGGAATCCCTCTCGTTGA
AATCGCCACCGCTCCCGATATTAAATCCCCAAGGCATGCACGTGAGGTTGCGGAATATATAGGAATGGTTCTCAGGTCTA
CAGGGAAGGTAAAAAGAGGGCTGGGCACAATCAGGCAGGATGTCAATATCTCAATTGCCAGAGGCGCAAGGGTTGAAATC
AAAGGGGTTCAGGCTCTTGACCTTATAGAAGACATTGTCCGCAAGGAAGTTGAAAGGCAGTTAAACCTTCTCTTTATCAG
GCAGGAACTTCTTGAAAGAAAAGCCTTTGTCTGTGAAGAAATCTATGATGCAACAGGGCTTTTTGTGGATACCAAATCCA
AAGTCCTGCAGAAAGGAATAAAAAAGGGAGCGATCCTGGCTGCCCGCCTGAGGAAATTCAACGGGCTTGTGGGCAGGGAG
GTCCAGCCTGGAAGAAGGCTCGGGACTGAGTTTTCGGACAGGGCAAAAACTGCAGGTGTTGGGGGAATCTTCCATACAGA
CGAACTTCCTAATTACGGGATAACAGAAAAGGAAGTGCAGGCTTTAAGGGACGCTATAGGTGCAAACCCGGACGATGCAA
TTATCATGGTTGCGGATGAGCCCGAAAAATCCAGGCTTGCAATCGAAGCGGTGATTCTCAGAGCAAAAGAAGCTATCGAG
GGAATCCCCGAGGAGACCAGAAAAGCCCTGCCTGATGGAAACACTGCTTACATGCGTCCCCTGCCAGGTGCAGCAAGAAT
GTACCCTGAAACCGATGTTCCTCAGATAGAGATCTCGCAGGAATACTTTGATTCCATAGAAGTCCCGGAGCTCCTTACGG
AAAGGGCAAAGAGGTTTGCTTCCGAAAGCGGCCTGAATAAAGAACTTGCTGAAAAGATAGCTTATTCGAAATACCTTCCT
CTTTTCGAGGCTTTACTGGATACCTACAGCGATAATGAACACGTTAATTCAACACTGATTGCCAGGACACTTGTAGGAAT
TGTCCCTGAGATCAGAAGAAACGGGGCTGAAACTGACAACCTTACGGATGAGCACTTCAGTGGGGTTTTTGCAGCAATAT
CAAATCAGGAAATCGCAAAAGAAGCCATCCAGGATCTTTTGACCGCACTTGCAAAGGAGCCTGAAATAACAGTCCAGCAG
GCGATTTCAAATCTCGGCCTGAGCGCCTTTGATCCCGAAGAAATCGAAAATTTCATTAAAAATATGGTCCGGGAGAAAGG
AGATTTCATTAAAGAAAAAGGCCCTGCAGCCCTTGGCCCTCTTATGGGCATTGTCATGAAAGAGTTCAGAGGAAAGGTTG
ACGGAAAGATCCTCAGCCATATGCTGAAAAGAGAGATAGATAGCTTTATTAATCAGGGGTAA

Upstream 100 bases:

>100_bases
GGAAAGCCAGGGTAAAGCTGAGGGAAATTAAAGAAATCAATGAAAAACCAGAGAGAGTTAAAAGGATCACGGCGCTTCCT
GCTTAGATTCGGGTGAAATC

Downstream 100 bases:

>100_bases
TCCCTGATCTTTTTTCCCATTTTTTCCGCTATTTTTTGTTATTTTTTCTTCTATTTTTTCTGCTACTTTTTGTTATTTTT
TCTTCTATTTTTTCCGCTAT

Product: glutamyl-tRNA(Gln) amidotransferase subunit E

Products: NA

Alternate protein names: Glu-ADT subunit E [H]

Number of amino acids: Translated: 633; Mature: 633

Protein sequence:

>633_residues
MEKYDYSELGLKAGLEIHQQLDSKEKLFCRCPTLIRDIEDSDFEFFRYLRATESEMGEKDRAAVEQTKIRRKYIYKAYDT
TCLVENDEEPPGELNREALDISLGVAKLFNMKPVDQMHVMRKIVVDGSNTSGFQRTAFLASDGNIETSEGRCRIDSLCVE
EEAAQKIEEIGDSIIYSLDRLGIPLVEIATAPDIKSPRHAREVAEYIGMVLRSTGKVKRGLGTIRQDVNISIARGARVEI
KGVQALDLIEDIVRKEVERQLNLLFIRQELLERKAFVCEEIYDATGLFVDTKSKVLQKGIKKGAILAARLRKFNGLVGRE
VQPGRRLGTEFSDRAKTAGVGGIFHTDELPNYGITEKEVQALRDAIGANPDDAIIMVADEPEKSRLAIEAVILRAKEAIE
GIPEETRKALPDGNTAYMRPLPGAARMYPETDVPQIEISQEYFDSIEVPELLTERAKRFASESGLNKELAEKIAYSKYLP
LFEALLDTYSDNEHVNSTLIARTLVGIVPEIRRNGAETDNLTDEHFSGVFAAISNQEIAKEAIQDLLTALAKEPEITVQQ
AISNLGLSAFDPEEIENFIKNMVREKGDFIKEKGPAALGPLMGIVMKEFRGKVDGKILSHMLKREIDSFINQG

Sequences:

>Translated_633_residues
MEKYDYSELGLKAGLEIHQQLDSKEKLFCRCPTLIRDIEDSDFEFFRYLRATESEMGEKDRAAVEQTKIRRKYIYKAYDT
TCLVENDEEPPGELNREALDISLGVAKLFNMKPVDQMHVMRKIVVDGSNTSGFQRTAFLASDGNIETSEGRCRIDSLCVE
EEAAQKIEEIGDSIIYSLDRLGIPLVEIATAPDIKSPRHAREVAEYIGMVLRSTGKVKRGLGTIRQDVNISIARGARVEI
KGVQALDLIEDIVRKEVERQLNLLFIRQELLERKAFVCEEIYDATGLFVDTKSKVLQKGIKKGAILAARLRKFNGLVGRE
VQPGRRLGTEFSDRAKTAGVGGIFHTDELPNYGITEKEVQALRDAIGANPDDAIIMVADEPEKSRLAIEAVILRAKEAIE
GIPEETRKALPDGNTAYMRPLPGAARMYPETDVPQIEISQEYFDSIEVPELLTERAKRFASESGLNKELAEKIAYSKYLP
LFEALLDTYSDNEHVNSTLIARTLVGIVPEIRRNGAETDNLTDEHFSGVFAAISNQEIAKEAIQDLLTALAKEPEITVQQ
AISNLGLSAFDPEEIENFIKNMVREKGDFIKEKGPAALGPLMGIVMKEFRGKVDGKILSHMLKREIDSFINQG
>Mature_633_residues
MEKYDYSELGLKAGLEIHQQLDSKEKLFCRCPTLIRDIEDSDFEFFRYLRATESEMGEKDRAAVEQTKIRRKYIYKAYDT
TCLVENDEEPPGELNREALDISLGVAKLFNMKPVDQMHVMRKIVVDGSNTSGFQRTAFLASDGNIETSEGRCRIDSLCVE
EEAAQKIEEIGDSIIYSLDRLGIPLVEIATAPDIKSPRHAREVAEYIGMVLRSTGKVKRGLGTIRQDVNISIARGARVEI
KGVQALDLIEDIVRKEVERQLNLLFIRQELLERKAFVCEEIYDATGLFVDTKSKVLQKGIKKGAILAARLRKFNGLVGRE
VQPGRRLGTEFSDRAKTAGVGGIFHTDELPNYGITEKEVQALRDAIGANPDDAIIMVADEPEKSRLAIEAVILRAKEAIE
GIPEETRKALPDGNTAYMRPLPGAARMYPETDVPQIEISQEYFDSIEVPELLTERAKRFASESGLNKELAEKIAYSKYLP
LFEALLDTYSDNEHVNSTLIARTLVGIVPEIRRNGAETDNLTDEHFSGVFAAISNQEIAKEAIQDLLTALAKEPEITVQQ
AISNLGLSAFDPEEIENFIKNMVREKGDFIKEKGPAALGPLMGIVMKEFRGKVDGKILSHMLKREIDSFINQG

Specific function: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-ph

COG id: COG2511

COG function: function code J; Archaeal Glu-tRNAGln amidotransferase subunit E (contains GAD domain)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatE subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004115
- InterPro:   IPR023168
- InterPro:   IPR004414
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02938 GAD; PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 70805; Mature: 70805

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKYDYSELGLKAGLEIHQQLDSKEKLFCRCPTLIRDIEDSDFEFFRYLRATESEMGEKD
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHCCCHH
RAAVEQTKIRRKYIYKAYDTTCLVENDEEPPGELNREALDISLGVAKLFNMKPVDQMHVM
HHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHCHHHHHHHHHHHHHHCCCCHHHHHHH
RKIVVDGSNTSGFQRTAFLASDGNIETSEGRCRIDSLCVEEEAAQKIEEIGDSIIYSLDR
HHHHHCCCCCCCCEEEEEEECCCCEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGIPLVEIATAPDIKSPRHAREVAEYIGMVLRSTGKVKRGLGTIRQDVNISIARGARVEI
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEECCCEEEE
KGVQALDLIEDIVRKEVERQLNLLFIRQELLERKAFVCEEIYDATGLFVDTKSKVLQKGI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHH
KKGAILAARLRKFNGLVGREVQPGRRLGTEFSDRAKTAGVGGIFHTDELPNYGITEKEVQ
HHHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHCCCCCCEECCCCCCCCCCHHHHH
ALRDAIGANPDDAIIMVADEPEKSRLAIEAVILRAKEAIEGIPEETRKALPDGNTAYMRP
HHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEECC
LPGAARMYPETDVPQIEISQEYFDSIEVPELLTERAKRFASESGLNKELAEKIAYSKYLP
CCCHHHCCCCCCCCCEEECHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LFEALLDTYSDNEHVNSTLIARTLVGIVPEIRRNGAETDNLTDEHFSGVFAAISNQEIAK
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHH
EAIQDLLTALAKEPEITVQQAISNLGLSAFDPEEIENFIKNMVREKGDFIKEKGPAALGP
HHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LMGIVMKEFRGKVDGKILSHMLKREIDSFINQG
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKYDYSELGLKAGLEIHQQLDSKEKLFCRCPTLIRDIEDSDFEFFRYLRATESEMGEKD
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHCCCHH
RAAVEQTKIRRKYIYKAYDTTCLVENDEEPPGELNREALDISLGVAKLFNMKPVDQMHVM
HHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHCHHHHHHHHHHHHHHCCCCHHHHHHH
RKIVVDGSNTSGFQRTAFLASDGNIETSEGRCRIDSLCVEEEAAQKIEEIGDSIIYSLDR
HHHHHCCCCCCCCEEEEEEECCCCEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGIPLVEIATAPDIKSPRHAREVAEYIGMVLRSTGKVKRGLGTIRQDVNISIARGARVEI
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEECCCEEEE
KGVQALDLIEDIVRKEVERQLNLLFIRQELLERKAFVCEEIYDATGLFVDTKSKVLQKGI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHH
KKGAILAARLRKFNGLVGREVQPGRRLGTEFSDRAKTAGVGGIFHTDELPNYGITEKEVQ
HHHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHCCCCCCEECCCCCCCCCCHHHHH
ALRDAIGANPDDAIIMVADEPEKSRLAIEAVILRAKEAIEGIPEETRKALPDGNTAYMRP
HHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEECC
LPGAARMYPETDVPQIEISQEYFDSIEVPELLTERAKRFASESGLNKELAEKIAYSKYLP
CCCHHHCCCCCCCCCEEECHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LFEALLDTYSDNEHVNSTLIARTLVGIVPEIRRNGAETDNLTDEHFSGVFAAISNQEIAK
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHH
EAIQDLLTALAKEPEITVQQAISNLGLSAFDPEEIENFIKNMVREKGDFIKEKGPAALGP
HHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LMGIVMKEFRGKVDGKILSHMLKREIDSFINQG
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]